• 미생물학회지
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• 제23권2호
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• pp.122-128
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• 1985

Bacillus thuringiensis serovar israelensis 4Q1 contains 8 different covalently closed-circular (CCC) plasmids of molecular weight 204, 267, 109, 103, 16, 7.6, 6.4, and 5.0kb. The three smallest plasmids, designated pBti6, and pBti8 may prove to be useful as cloning vectors because of thier size and ease of isolation. The three plasmids were incubated separately with 9 different restriction enzymes and 7 of the enzymes tested cleaved one or more of the plasmids. Plasmid pBti6 has a single site for Bg1 II, Pst I and Pvu II, two sites for Bc1 I and Eco RI, and five sites for Hind III. Plasmid pBti7 has a single site for Bam HI and Pst I, two sites for Hind III, and three sites for PvuII. Plasmic pBti8 has a single site for Bam HI, BelI and Hind III, two sites for Eco RI, and three sites for Bgl II and Pvu II. Composite restriction enzyme maps for pBti6, pBti7 and pBti8 were constructed. The sites of restriction enzyme cleavage were determined by single, double and partial digests of the plasmid DNA. All the restriction sites were aligned relative to the single Bgl II(pBti6), Pst I(pBti7) or Hind III(pBti8) site, respectively.

• 농업과학연구
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• 제44권3호
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• pp.318-324
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• 2017

With the development of next-generation sequencing (NGS), a cutting-edge technology, genotype-by-sequencing (GBS) became available at a low cost per sample. GBS makes it possible to customize the process of library preparation to obtain high-quality single nucleotide polymorphisms (SNPs) in the most efficient way. However, a GBS library is hard to construct due to fine-tuning of concentration of each reagent and set-up. The major reason for this is the presence of undigested genomic DNA (gDNA) owing to the efficiency of different restriction enzymes for different species with unknown reasons. Therefore, this proof-concept study is to demonstrate the unpredictable patterns of enzyme digestion from various plants in order to make the reader aware of the caution needed when choosing restriction enzymes for their GBS library preparations. Indeed, no pattern was found for the digestibility of gDNA samples and restriction enzymes in the current study. We suggest that more data should be accumulated on this matter to help researchers who want to apply GBS technologies in a variety of genetic approaches.

• Journal of Microbiology
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• 제41권4호
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• pp.312-319
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• 2003

In an attempt to develop a method for rapid and accurate identification of six Vibrio species that are clinically important and most frequently detected in Korea, 16S rDNA restriction fragment length polymorphism (RFLP) of Vibrio type strains, as well as environmental isolates obtained from the Korean coastal area, was analyzed using ten restriction endonucleases. Digestion of the 16S rDNA fragments amplified by polymerase chain reaction (PCR) with the enzymes gave rise to 2~6 restriction patterns for each digestion for 47 Vibrio strains and isolates. An additional 2~3 restriction patterns were observed for five reference species, including Escherichia coli, Aeromonas hydrophila, A. salmonicida, Photobacterium phosphoreum, and Plesiomonas shigelloides. A genetic distance tree based on RFLP of the bacterial species correlated well with that based on 16S rDNA sequences. The very small 16S rDNA sequence difference (0.1%) between V. alginolyticus and V. parahaemolyticus was resolved clearly by RFLP with a genetic distance of more than 2%. RFLP variation within a species was also detected in the cases of V. parahaemolyticus, V. proteolyticus, and V. vulnificus. According to the RFLP analysis, six Vibrio and five reference species were assigned to 12 genotypes. Using three restriction endonucleases to analyze RFLP proved sufficient to identify the six pathogenic Vibrio species.

• 한국식품영양과학회지
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• 제20권2호
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• pp.187-196
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• 1991

Aging is the progressive accumulation of changes with time associated with responsible for the ever-increasing susceptibility to disease and death which accompanies advancing age. Lipid peroxides easily produced in the membrane system by the chain reaction of free radicals which occurred from various environmental factors. The amount of lipid peroxides produced in biological system increased with aging process, and lipid peroxidation damages involved in aging process and pathological disorders. Although lipid peroxides have such deleterious effects on the organisms, there are numerous substances and mechanisms which prevent the reaction of peroxide formation and protect the subject from its toxicity. This review provides an overview of how does lipid peroxidation of unsaturated lipids take place by free radical, and what is the intervention of lipid peroxides in pathogenesis of some human diseases, and also how does food restriction influences the aging process and various pathological disorders. The major focus of this paper is to review the evidence indicating that food restriction retards the aging process, and possible mechanisms of its actions. Therefore, it discussed the effects of age and food restriction on life-span, membrane yield, lipid peroxidation, fatty acid composition and peroxidizability, cholesterol and triglyceride levels, prostaglasndin and thromboxane synthesis, which may be concerned with blood flow, membrane fluidity, homeostasis and glomerular filtration rate in living body.

• 한국응급구조학회지
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• 제24권3호
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• pp.89-106
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• 2020

Purpose: This study attempts to improve the status of emergency care for major trauma patients transferred by 119 paramedics by analyzing the status of emergency care and the obstacles to the spinal motion restriction (SMR) for major trauma patients. Methods: A total of 600 rescue logs were collected from major trauma patients transported by 119 paramedics in the C fire department from Jan. 1, 2015, to Dec. 31, 2017. And then, 280 questionnaires were collected from the 119 paramedics in C fire department from May 3 to Jun. 3, 2019. Data were analyzed using SPSS 24.0 version. Results: Among 499 spinal motion restriction adaptive patients, the spinal motion restriction rate was 51.1% (255 individuals). Lack of human resources and quality control problems were among the obstacles to spinal motion restriction. Conclusion: The 119 paramedics should improve their activeness and skills in performing emergency care, and since training and experience are of crucial importance, they should expand various education systematized according to demand.

• 대한물리치료과학회지
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• 제29권2호
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• pp.48-56
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• 2022

Background: This study was to identify the effects of blood flow restriction on gluteus muscles thickness, density, and white area index for children with cerebral palsy. Design: Randomized controlled trial. Methods: Twenty children with cerebral palsy were assigned randomly to an experimental (n=10) or control (n=10) group. The experimental group performed a bicycle exercise after blood flow restriction. The control group performed a bicycle exercise. The study used an ultrasonic instrument to measure gluteus muscles thickness, density, and WAI. Wilcoxon signed ranks test was used for determination of differences before and after treatment, and a Mannn-whitney U test was used for determination of differences between treatment groups. Results: As a result of comparison within groups, the experimental and control group showed significant difference for gluteus muscles thickness, density, and WAI after the experiment (p<0.05). In comparison between two groups, the experimental group showed more significant difference in gluteus muscles thickness, density, and WAI than the control group (p<0.05). Conclusion: Based on these results, a bicycle exercise after blood flow restriction effectively improved the gluteus muscles thickness, density, and WAI of children with cerebral palsy.

• PNF and Movement
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• 제20권1호
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• pp.1-8
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• 2022

Purpose: The purpose of this study was to investigate the effect of blood flow restriction bridge exercise on leg muscle thickness and balance. In addition, it is to promote blood flow restriction exercise as the basis for early prevention, diagnosis, and treatment of sarcopenia in clinical practice. Methods: Twenty elderly women aged 65 years or older were selected to participate in this study. The subjects were divided into two groups of 10: one with blood flow restriction with bridge exercise (BFRG) and the other with bridge exercise alone (BG). As for the exercise method, the thickness of rectus femoris and vastus medialis and Berg balance scale were investigated by intervention for 30 minutes a day, 3 times a week, for a total of 6 weeks. Results: There was significant difference in the thickness of the rectus femoris and vastus medial and within-group changes in the Berg balance scale (p <0.05) before and after the experiment in the BFRG and BG groups (p<0.05). There was a significant difference in change between the two groups (p <0.05). Conclusion: There was a significant difference in the intragroup change of the Berg balance scale in the BFRG before and after the experiment, but there was no significant difference in the BG, and there was no significant difference in the change between the two groups.

• PNF and Movement
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• 제20권1호
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• pp.31-39
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• 2022

Purpose: The purpose of this study was to identify the effects of general exercise after blood flow restriction on trunk muscles thickness in children with spastic cerebral palsy. Methods: Twenty children with cerebral palsy were assigned randomly to an experimental (n = 10) or a control (n = 10) group. The experimental group performed general exercise after blood flow restriction, while the control group performed general exercise alone. The study used an ultrasonic instrument to measure trunk muscles thickness. The Wilcoxon signed-rank test was used to determine differences before and after treatment, and the Mann-Whitney U test was used to determine differences between treatment groups. Results: From a comparison within the groups, the experimental and control groups showed significant difference in trunk muscle thickness after the experiment (p < 0.05). In a comparison between the two groups, the experimental group showed more significant difference in trunk muscle thickness than the control group (p < 0.05). Conclusion: Based on these results, general exercise after blood flow restriction effectively improves trunk muscle thickness in children with cerebral palsy.

• 대한수학회논문집
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• 제20권1호
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• pp.35-49
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• 2005

For an extension field K of k, a restriction homomorphism on Brauer k-group B(k) maps Brauer k-algebras to Brauer K- algebras by tensor product. A purpose of this work is to study the restriction map that sends radical (Schur) k-algebras to radical (Schur) K-algebras. And we ask an analogous question with respect to corestriction map on Brauer group B(K) that whether the corestriction map sends radical K-algebras to radical k-algebras.

• Journal of applied mathematics & informatics
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• 제13권1_2호
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• pp.457-470
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• 2003

The comparison of two or more Lorenz curves of Pareto distributions of first kind under arbitrary order restriction is studied. The problem is turned out to be a statistical inference problem concerning scale parameters under order restriction. We assume that the location parameters of Palate distributions are completely unknown. In this paper the maximum likelihood estimation and likelihood ratio tests for and against order restriction are proposed.