• Title/Summary/Keyword: restriction

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Restriction Fragment Fingerprint of an Alkaliphilic Micrococcus sp. Y-1 Genome by Pulsed-field Gel Electrophoresis

  • Kim, Cheorl-Ho
    • Journal of Microbiology and Biotechnology
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    • v.5 no.1
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    • pp.1-5
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    • 1995
  • A genomic DNA of alkaliphilic bacterium, Micrococcus sp. Y-l, was analysed using the physical mapping method of pulsed-field gel electrophoresis (PFGE). Five restriction enzymes of Sspl, Hpal, Xbal, Ndel or EcoRI, which recognize the Adenine-Thymine-rich sequences of genomic DNA, were used for the generation of few (7 to 20) distinctly separate fragments, with average sizes in the range of 200~500 kb. However, the sites for Notl and SfiI, 8 base-recognizing enzymes, were highly frequent. The genome size of this strain was determined to be 4 mega base pairs (Mb) from restriction fragments separated by PFGE. This is the first case of restriction mapping in alkaliphilic bacterium.

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A Study on the Estimation Method of Compensation for Restriction in Licensed Fisheries Caused by a Large Scale Coastal Reclamation (대규모 연안매립으로 인한 허가어업제한보상액 산출방식에 관한 일고찰)

  • 강용주;김기수;하강렬
    • The Journal of Fisheries Business Administration
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    • v.28 no.1
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    • pp.71-83
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    • 1997
  • This paper tries to suggest one kind of estimation method of compensation for restriction in licenced fisheries caused by a large scale coastal reclamation under Code 62 of the Fisheries Act. To do so, the paper focuses on estimating the restriction rate of licenced fisheries through introducing several new factors for calculating the amount value of compensation, such as the restriction rate of fishing area, the decreasing rate of fishing time and the variation rate of fishing cost. Finally, the paper tries to apply the method to the case of compensation fer fisheries damage at the Incheon coastal area caused by the construction of the base of LNG of 1992.

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Shape deformation and wear sensation in wearing on support type panty stocking (고탄력 팬티스타킹 착용에 의한 형태변형 및 착용감)

  • 류현혜;성수광
    • Journal of the Ergonomics Society of Korea
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    • v.14 no.1
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    • pp.37-57
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    • 1995
  • The purpose of this study was to investigate the elastic effect of deformation and restriction in wearing a support type panty stocking (PS). The study investigated deformation rate in all surface area, deformation rate in local area, length deformation rate, changes in slip and feeling of restriction under the standard laboratory condition. Two healthy adult females in twenties wore four types of PS. The result were as follows : The deformation rate in all surface area was 117 .approx. 132% in wearing support type PS, 157% in wearing mono type PS, due to the intensities of restriction. The deformation rate in local area was in order, course > oblique > wale direction. The largest deformation rate was observed in hip girth. Between the deformation rates in all surface and local areas was a high correlation acknowledged, especially the course direction showed a high correlation. Support type PSs showed more deformation and changes of slip than wool and mono type PS. Especially, changes of slip at the knee appeared greatly. The responses of restriction evaluated from a paired comparison method were in order, JS 2 > KS 4 > KS 9 > KS 5 > KS 1 > KM 3.

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Restriction Endonuclease DNA Analysis of Leptospiral Field Isolates from Korea (제한효소 DNA 분석법에 의한 국내분리 렙토스피라균의 동정)

  • Chang, Woo-Hyun;Kim, Suck-Yong;Seo, Jeong-Sun
    • The Journal of the Korean Society for Microbiology
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    • v.22 no.4
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    • pp.463-471
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    • 1987
  • The genomes of leptospiral field isolates from Korea belonging to serogroup Icterohaemorrhagiae (21 strains) and serogroup Canicola (1 strain) were analysed and compared by restriction enzyme analysis with EcoRI and HindIII as digesting enzymes. One isolate belonging to serogroup Canicola showed the same pattern as serovar portlandvere. All 21 isolates belonging to serogroup Icterohaemorrhagiae showed almost same patterns as Leptospira serovar lai from China, But with very slight differences 21 isolates could be classified into 8 subtypes and these grouping seems to reflect the differences in epidemiological niche. And also the geographical data consisted with the grouping into 8 subtypes. According to our results, we concluded that the restriction endonuclease analysis of chromosomal DNA will be an accurate and reliable method to compare and classify pathogenic leptospires.

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Numerical Identification of a Streptomyces Strain Producing Restriction Endonuclease Sdi I (제한효소인 Sdi I을 생성하는 Streptomyces 분리 균주의 수리동정)

  • Bae, Moo;Suh, Won-Na;Song, Eun-Sook;Lee, Kye-Joon
    • Microbiology and Biotechnology Letters
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    • v.22 no.2
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    • pp.126-133
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    • 1994
  • Numerical identification was aplied for Streptomyces sp.264, an isolate producing a new restriction endonuclease Sdi I. The restriction enzyme would appear to be an isoschisomer of Xho I. Fifty taxonomic unit characters were tested and the data obtained were analyzed numerically by using the TAXON program. The isolate was identified to be the major cluster 19 of Streptomyces and best matched to S. diastatochromogenes. It was, therefore, concluded that the isolate was identified to be a member of Streptomyces diastatochromogenes.

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Characterization of Tetracycline Resistance Plesmid of Multidrug-resistant Staphylococcus aureus (다제내성 황색포도상구균이 가지고 있는 테트라사이클린 내성 플라스미드의 동정)

  • 이대운;문경호
    • YAKHAK HOEJI
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    • v.39 no.1
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    • pp.6-9
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    • 1995
  • The clinical isolate Staphylococcus aureus SA2 had four kinds of plasmids and was resistant to ampicillin, chloramphenicol, clindamycin, erythromycin, gentamicin, kanamycin, methicillin, streptomycin, tetracycline and tobramycin. Transformation experiment demonstrated that 4.44 kb plasmid(pKH6) encoded resistance to tetracycline. The cleavage map of pKH6 was determined by restriction enzyme mapping techniques. The cleavage map is given for EcoRV, HindIII, HpaI, HpaII, KpnI and Xbal. Restriction endonucleases BamHl, BglI, BGIII, BstEII, EcoRI, HaellI, PstI, PvuII, SalI, Smal, and Xhol have no site on this plasmid. The restriction map revealed extensive structural homology between pKH6 and pT181.

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Definiteness Effect: Definiteness Restriction and Indefiniteness Restriction (한정성 효과: 한정성 제약과 비한정성 제약)

  • 전영철
    • Language and Information
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    • v.6 no.2
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    • pp.83-104
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    • 2002
  • I argue that Definiteness Effect (DE) should include Indefiniteness Restriction (IR) as well as Definiteness Restriction (DR). DR is exhibited by existential constructions, predicate nominals, inalienable possession constructions, and verbs with semantic features like 〔((CAUSE TO) COME TO) EXIST〕. IR is caused by some existence presupposition of aspectual adverbs, aspectual verbs, repetitives, and topic markers. The environments for DR and IR determine the (in)definiteness of Korean bare noun phrases which otherwise can be used either way. The neutralization of DR is also induced by focus which imposes a certain amount of structure on the event quantification. Van der Sandt's (1992) Presuppositions-as- Anaphora-Theory is effectively used to account for those examples from DR, ID, and the neutralization of BR.

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A Study on Chargin and Discharging Characteristics of Variable Volume with Compressed Air (가변체적내의 압축공기 충진 및 방출특성연구)

  • Kim, Dong-Soo;Kim, Hyoung-Eui;Park, Jae-Bum;Kang, Bo-Sig;Sung, Baek-Ju
    • Journal of the Korean Society for Precision Engineering
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    • v.12 no.11
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    • pp.125-131
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    • 1995
  • Pneumatic System has been mainly used as main equipment for actuation and control of fluid force in manufacturing industry. For velocity control of piston, meter-out restriction method is used in many cases. In this systems, meter-out restriction method is adopted for analysing the Dynamic Charging and Discharging Process which is Variable Volume Chamber. Experiments has been conducted for different supply pressure condition. As a experimental result, charge side chamber pressure rises to supply pressure rapidily and discharge side chamber pressure decreases. Also, when the air in the cylinder is discharged, tempdrature of air decreases steeply. Restriction of the Cylinder sometimes freeze and it dose not function. The result will be useful for the analysis of pneumatic system.

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Brief study on Restriction of Hazardous Substances Directive (RoHS) (유해물질(有害物質) 제한지침(制限指針)(Restriction of Hazardous Substances Directive, RoHS)의 현황(現況))

  • Kumar, J. Rajesh;Lee, Jin-Young;Kim, Joon-Soo;Shhn, Jeong-Soo
    • Resources Recycling
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    • v.17 no.6
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    • pp.3-9
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    • 2008
  • The Directive on the Restriction of the Use of Certain Hazardous Substances in Electrical and Electronic Equipment commonly referred to as the Restriction of Hazardous Substances Directive or RoHS was adopted in February 2003 by the European Union. The RoHS directive took effect on 1 July 2006, and is required to be enforced and become law in each member state. This directive restricts the use of six hazardous materials in the manufacture of various types of electronic and electrical equipment. It is closely linked with the waste electrical and electronic equipment directive (WEEE) 2002/96/EC which sets collection, recycling and recovery targets for electrical goods and is part of a legislative initiative to solve the problem of huge amounts of toxic e-waste.