• 한국동물생명공학회지
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• 제34권1호
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• pp.2-9
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• 2019

Because mesenchymal stem cells (MSCs) maintain distinct capacities with respect to self-renewal, differentiation ability and immunomodulatory function, they have been highly considered as the therapeutic agents for cell-based clinical application. Of particular, differentiation condition alters characteristics of MSCs, including cellular morphology, expression of gene/protein and cell surface molecule, immunological property and apoptosis. However, the previous results for differentiation-related apoptosis in MSCs have still remained controversial due to varied outcomes. Therefore, the present study aimed to disclose periodical alterations of pro- and anti-apoptosis in MSCs under differentiation inductions. The human dental pulp-derived MSCs (DP-MSCs) were differentiated into adipocytes and osteoblasts during early (1 week), middle (2 weeks) and late (3 weeks) stages, and were investigated on their apoptosis-related changes by Annexin V assay, qRT-PCR and western blotting. The ratio of apoptotic cell population was significantly (p < 0.05) elevated during the early to middle stages of differentiations but recovered up to the similar level of undifferentiated state at the late stage of differentiation. In the expression of mRNA and protein, whereas expressions of pro-apoptosis-related makers (BAX and BAK) were not altered in any kind and duration of differentiation inductions, anti-apoptosis marker (BCL2) was significantly (p < 0.05) elevated even at the early stage of differentiations. The recovery of apoptotic cell population at the late stage of differentiation is expected to be associated with the response by elevation of anti-apoptotic molecules. The present study may contribute on understanding for cellular mechanism in differentiation of MSCs and provide background data in clinical application of MSCs in the animal biotechnology to develop effective and safe therapeutic strategy.

• 한국안전학회지
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• 제34권1호
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• pp.27-33
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• 2019

Combustion characteristics of gasoline/ethanol fuel were investigated both numerically and experimentally for vehicle fire safety. The numerical simulation was performed on the well-stirred reactor (WSR) to simulate the homogeneous gasoline engine and to clarify the effect of ethanol addition in the gasoline fuel. The simulating cases with three independent variables, i.e. ethanol mole fraction, equivalence ratio and residence time, were designed to predict and optimized systematically based on the response surface method (RSM). The results of stoichiometric gasoline surrogate show that the auto-ignition temperature increases but NOx yields decrease with increasing ethanol mole fraction. This implies that the bioethanol added gasoline is an eco-friendly fuel on engine running condition. However, unburned hydrocarbon is increased dramatically with increasing ethanol content, which results from the incomplete combustion and hence need to adjust combustion itself rather than an after-treatment system. For more tangible understanding of gasoline/ethanol fuel on pollutant emissions, experimental measurements of combustion products were performed in gasoline/ethanol pool fires in the cup burner. The results show that soot yield by gravimetric sampling was decreased dramatically as ethanol was added, but NOx emission was almost comparable regardless of ethanol mole fraction. For soot morphology by TEM sampling, the incipient soot such as a liquid like PAHs was observed clearly on the soot of higher ethanol containing gasoline, and the soot might be matured under the undiluted gasoline fuel.

• 반도체디스플레이기술학회지
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• 제18권1호
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• pp.15-20
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• 2019

Inspection and measurement of surface quality is one of the most critical processes for manufacturing products such as semiconductor wafers, sapphire substrates, and display panels. The vibrations of the inspection and measurement devices are supposed to be the most dominant factors for severe measurement errors and longer measuring time. In this study, dynamic characteristics of an inspection and measurement device are analyzed through frequency response experiment and computer simulation to obtain parameters such as frequencies, magnitudes, mode shapes, and periods of vibrations. And then an active damper which consists of sensor, interface board, and actuator is designed based on the parameters to formulate the most effective reaction signal to suppress the vibrations which is generated by an interface board, and provided by an actuator. If the vibrations are measured by the sensor, the active damper immediately generates and provides the corresponding reaction signal to inspection and measurement device. The result shows that the active damper can suppress structural vibrations effectively and reduce measuring time of the device and enhance the productivity.

• 한국물환경학회지
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• 제34권6호
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• pp.591-601
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• 2018

Optimized BMP plans for controlling water quality using the Pareto trade-off surface curve in upper basin of Namgang Dam is proposed. The proposed alternatives consist of BMP installation scenarios in which the reduction efficiency of non-point pollutants is maximized in a given budget. The multi-objective optimization process for determining the optimal alternatives was performed without direct implementation of a watershed model such as SWAT analysis, thereby reducing the time taken. The shortening of the calculation time further enhances the applicability of the multi-objective optimization technique in preparing regional water quality management alternatives. In this study, different types of BMP are applied depending on the land use conditions. Fertilizer input control and vegetative filter strip are considered as alternatives to applying BMP to the field but only control of fertilizer input can be applied to rice paddies. Fertilizer input control and vegetative filter strip can be installed separately or simultaneously in a hydrologic response unit. Finally, 175 BMP application alternatives were developed for the water quality management of the upper river basin of Namgang dam. The proposed application alternative can be displayed on the map, which has the advantage of clearly defining the BMP installation location.

• 한국음향학회지
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• 제38권3호
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• pp.321-327
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• 2019

본 연구의 목표는 의류 건조기용 원심팬과 덕트 및 하우징 등을 포함한 공기배출 시스템의 유량 성능 향상 및 공력소음을 저감하기 위한 것이다. 전산유체역학과 FW-H(Ffowcs-Williams and Hawkings) 방정식에 기초한 음향상사법을 이용하여 유동 및 소음 특성을 고찰하였다. 유량과 소음성능 최적화 설계를 위해 반응표면기법을 활용하였다. 설계 인자로 원심팬의 날개 입구각, 출구각을 고려한 2인자 중심합성계획법을 채택하였다. 도출된 최적설계안은 덕트와 하우징에서 감소된 난류에너지 분포를 나타냈으며 결과적으로 유량의 증가와 공력소음이 감소됨을 확인하였다. 최종적으로 최적설계안을 기초로 제작한 시제품에 대한 실험을 통하여 4.2 % 유량이 증가함을 확인하였다.

• Tissue Engineering and Regenerative Medicine
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• 제15권6호
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• pp.771-779
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• 2018

BACKGROUND: Mesenchymal stromal cells (MSCs) are multipotent stem cells that can differentiate into several cell types. In addition, many studies have shown that MSCs modulate the immune response. However, little information is currently available regarding the maintenance of immunomodulatory characteristics of MSCs through passages. Therefore, we investigated and compared cytokine and gene expression levels from adipose (AD) and bone marrow (BM)-derived MSCs relevant to immune modulation from early to late passages. METHODS: MSC immunophenotype, growth characteristics, cytokine expressions, and gene expressions were analyzed. RESULTS: AD-MSCs and BM-MSCs had similar cell morphologies and surface marker expressions from passage 4 to passage 10. Cytokines secreted by AD-MSCs and BM-MSCs were similar from early to late passages. AD-MSCs and BM-MSCs showed similar immunomodulatory properties in terms of cytokine secretion levels. However, the gene expressions of tumor necrosis factor-stimulated gene (TSG)-6 and human leukocyte antigen (HLA)-G were decreased and gene expressions of galectin-1 and -3 were increased in both AD- and BM-MSCs with repeated passages. CONCLUSION: Our study showed that the immunophenotype and expression of immunomodulation-related cytokines of AD-MSCs and BM-MSCs immunomodulation through the passages were not significantly different, even though the gene expressions of both MSCs were different.

• 한국재료학회지
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• 제31권10호
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• pp.552-561
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• 2021

For the purpose of manufacturing a high efficiency TiO₂ photocatalyst, B-doped TiO₂ photocatalysts are synthesized using a plasma electrolytic oxidation method in 0.5 M H₂SO₄ electrolyte with different concentrations of H₃BO₃ as additive. For the B doped TiO₂ layer fabricated from sulfuric electrolyte having a higher concentration of H₃BO₃ additive, the main XRD peaks of (101) and (200) anatase phase shift gradually toward the lower angle direction, indicating volume expansion of the TiO₂ anatase lattice by incorporation of boron, when compared with TiO₂ layers formed in sulfuric acid with lower concentration of additive. Moreover, XPS results indicate that the center of the binding energy peak of B1s increases from 191.45 eV to 191.98 eV, which suggests that most of boron atoms are doped interstitially in the TiO₂ layer rather than substitutionally. The B doped TiO₂ catalyst fabricated in sulfuric electrolyte with 1.0 M H₃BO₃ exhibits enhanced photocurrent response, and high efficiency and rate constant for dye degradation, which is ascribed to the synergistic effect of the new impurity energy band induced by introducing boron to the interstitial site and the improvement of charge transfer reaction.

• 한국의상디자인학회지
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• 제23권3호
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• pp.43-55
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• 2021

Unlike nail care, nail art is a field with strong decorative elements that decorate the surface of the nail. In line with the trend of the times, which freely expresses one's personality, nail art is establishing itself as a definite and a key area in the beauty industry. Currently, design creation and technology developments are important, with the release of various nail materials and new trends that spread through mass media. Despite the great developments in the nail beauty industry, there is a lack for nail artists. Due to this fact, it is considered a necessary to combine nail art with the contemporary art styles, which have various motifs of design, and to study nail art design using them. In response, this study selected art from the Rococo era, where delicate and colorful decorative elements were at their peak, to consider the artistic styles of the time and present them in various nail designs. This study would like to expand the scope of new nail designs by suggesting colorful and feminine designs to women, who are the main customers of nail salon. In addition, the purpose of this study is to provide nail artists with basic materials that can be used in creative ideas and expressions, such as 2D and 3D nail designs, and composite nail design art, which are the art areas for nail art.

• 한국해양공학회지
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• 제35권2호
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• pp.141-149
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• 2021

The A60 class deck penetration piece is a fire-resistance apparatus installed on the deck compartment to protect lives and to prevent flame diffusion in the case of a fire accident in a ship or offshore plant. In this study, the sensitivity of the fire-resistance performance and approximation characteristics for the A60 class penetration piece was evaluated by conducting a transient heat-transfer analysis and fire test. The transient heat-transfer analysis was conducted to evaluate the fire-resistance design of the A60 class deck penetration piece, and the analysis results were verified via the fire test. The penetration-piece length, diameter, material type, and insulation density were used as the design factors (DFs), and the output responses were the weight, temperature, cost, and productivity. The quantitative effects of each DF on the output responses were evaluated using the design-of-experiments method. Additionally, an optimum design case was identified to minimize the weight of the A60 class deck penetration piece while satisfying the allowable limits of the output responses. According to the design-of-experiments results, various approximate models, e.g., a Kriging model, the response surface method, and a radial basis function-based neural network (RBFN), were generated. The design-of-experiments results were verified by the approximation results. It was concluded that among the approximate models, the RBFN was able to explore the design space of the A60 class deck penetration piece with the highest accuracy.

• Structural Engineering and Mechanics
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• 제70권6호
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• pp.703-710
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• 2019

While fiber-reinforced plastic (FRP) materials have been largely used in the retrofitting of concrete buildings, its application has been limited because of some problems such as de-bonding of FRP layers from the concrete surface. This paper is the part of a wide experimental and analytical investigation about flexural retrofitting of reinforced concrete (RC) columns using FRP and mechanical fasteners (MF). A new generation of MF is proposed, which is applicable for retrofitting of RC columns. Furthermore, generally, to evaluate a retrofitted structure the nonlinear static and dynamic analyses are the most accurate methods to estimate the performance of a structure. In the nonlinear analysis of a structure, accurate modeling of structural elements is necessary for estimation the reasonable results. So for nonlinear analysis of a structure, modeling parameters for beams, columns, and beam-column joints are essential. According to the concentrated hinge method, which is one of the most popular nonlinear modeling methods, structural members shall be modeled using concentrated or distributed plastic hinge models using modeling parameters. The nonlinear models of members should be capable of representing the inelastic response of the component. On the other hand, in performance based design to make a decision about a structure or design a new one, numerical acceptance should be determined. Modeling parameters and numerical acceptance criteria are different for buildings of different types and for different performance levels. In this paper, a new method was proposed for FRP retrofitted columns to avoid FRP debonding. For this purpose, mechanical fasteners were used to achieve the composite behavior of FRP and concrete columns. The experimental results showed that the use of the new method proposed in this paper increased the flexural strength and lateral load capacity of the columns significantly, and a good composition of FRP and RC column was achieved. Moreover, the modeling parameters and acceptance criteria were presented, which were derived from the experimental study in order to use in nonlinear analysis and performance-based design approach.