• The Korean Journal of Physiology and Pharmacology
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• 제4권6호
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• pp.507-513
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• 2000

ErbB3/HER3 is a cell surface receptor which belongs to the ErbB/HER subfamily of receptor protein tyrosine kinases. When expressed in NIH/3T3 cells, ErbB3 can form heterodimeric coreceptor with endogenous ErbB2. Among known intracellular effectors of the ErbB2/ErbB3 are mitogen-activated protein kinase (MAPK) and phosphoinositide (PI) 3-kinase. In the present study, we studied relative contributions of above two distinct signaling pathways to the heregulin-induced mitogenic response via activated ErbB3. For this, clonal NIH-3T3 cell lines expressing wild-type ErbB3 and ErbB3 mutants were stimulated with $heregulin{\beta}_1$. While cyclin D1 level was markedly high and further increased by treatment of heregulin in cells expressing wild-type ErbB3, the elimination of either Shc binding or PI 3-kinase binding lowered both levels. This result was supported by the reduction of cyclin $D_1$ expression by preteatment with MAPK kinase inhibitor or PI 3-kinase inhibitor before stimulation with heregulin. In accordance with the cyclin $D_1$ expression, elimination of either Shc binding or PI 3-kinase binding reduced the heregulin-induced DNA synthesis and cell growth rate. Our results obtained by the comparison of wild-type and ErbB3 mutants indicate that the full induction of the cell cycle progression through $G_1/S$ phase by ErbB3 activation is dependent on both Shc/MAPK and PI 3-kinase signal transduction pathways.

• The Korean Journal of Physiology and Pharmacology
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• 제12권6호
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• pp.299-306
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• 2008

Sometimes, spinal cord injury (SCI) results in various chronic neuropathic pain syndromes that occur diffusely below the level of the injury. It has been reported that behavioral signs of neuropathic pain are expressed in the animal models of contusive SCI. However, the observation period is relatively short considering the natural course of pain in human SCI patients. Therefore, this study was undertaken to examine the time course of mechanical and cold allodynia in the hindpaw after a spinal cord contusion in rats for a long period of time (30 weeks). The hindpaw withdrawal threshold to mechanical stimulation was applied to the plantar surface of the hindpaw, and the withdrawal frequency to the application of acetone was measured before and after a spinal contusion. The spinal cord contusion was produced by dropping a 10 g weight from a 6.25 and 12.5 mm height using a NYU impactor. After the injury, rats showed a decreased withdrawal threshold to von Frey stimulation, indicating the development of mechanical allodynia which persisted for 30 weeks. The withdrawal threshold between the two experimental groups was similar. The response frequencies to acetone increased after the SCI, but they were developed slowly. Cold allodynia persisted for 30 weeks in 12.5 mm group. The sham animals did not show any significant behavioral changes. These results provide behavioral evidence to indicate that the below-level pain was well developed and maintained in the contusion model for a long time, suggesting a model suitable for pain research, especially in the late stage of SCI or for long term effects of analgesic intervention.

• 동력기계공학회지
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• 제20권2호
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• pp.51-57
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• 2016

Non-esterified biodiesel fuel is cheaper than esterified that because of a simple manufacturing process that only consists of filtering. Applicability of this on diesel engine with electronic control system was accomplished, then optimization adopting a fractional factorial design and response surface methodology was carried out at 25% and 50% of engine load in this study. Pressure of common rail and injection timing mainly effected on responses as specific fuel oil consumption and nitrogen oxides regardless of engine load. Estimations were 310.3 g/kWh of specific fuel oil consumption and 237 ppm of nitrogen oxides at 25% load, and 233.2 g/kWh of specific fuel oil consumption and 730 ppm of nitrogen oxides at 50% load. Tests to verify these estimations were accomplished and as the results, specific fuel oil consumption was 300.4 g/kWh and NOx was 277 ppm at 25% load and 236.8 g/kWh and 573 ppm at 50% load.

• 품질경영학회지
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• 제48권2호
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• pp.269-282
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• 2020

Purpose: The practical design for experiments with mixtures of q components is consisted in the four types of design points, vertex, center of edge, axial, and center points in a (q-1)-dimensional simplex space. We propose a sequential method for the successful construction of the design space in Quality by Design (QbD) by allowing the different number of replicates at the four types of design points in the practical design when the quadratic canonical polynomial model is assumed. Methods: To compare the mixture designs efficiency, fraction of design space (FDS) plot is used. We search for the practical mixture designs whose the minimal half-width of the tolerance interval per a standard deviation, which is denoted as d₂, is less than 4.5 at 0.8 fraction of the design space. They are found by adding the different number of replicates at the four types of the design points in the practical design. Results: The practical efficient mixture designs for the number of components between three and five are listed. The sequential method to establish a design space is illustrated with the two examples based on the simulated data. Conclusion: The designs with the center of edge points replications are more efficient than those with the vertex points replication. We propose the sample size of at least 23 for three components, 28 for four components, and 33 for the five components based on the list of efficient mixture designs.

• 대한방사선기술학회지:방사선기술과학
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• 제29권4호
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• pp.257-260
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• 2006

전신 PET/CT 검사에서 $^{18}F-FDG$의 방사성의약품을 투여한 환자가 방사선원이 되기에 환자로부터 종사자의 피폭선량 예측을 위한 PET 검사실에서 환자 주변의 공간선량률을 측정 분석하였다. 연간 개인피폭선량은 대학병원의 핵의학 분야에 근무하는 종사자가 방사선종양학과와 소규모병원의 진단방사선 분야에 비해 검사 중에 환자로부터 방출되는 공간선량률에 의하여 개인피폭선량이 높게 나타났다. 그리고 PET/CT 검사에서 $^{18}F-FDG$를 이용하는 경우에 $^{99m}Tc$ 보다 공간선량률이 $4{\sim}6$배 정도로 훨씬 높고, 촬영실 전체에 공간선량률이 분포함을 알 수 있었다. 따라서 방사성의약품 투여 후 안정실이나 PET 검사 중에 촬영실 내에서는 항상 방사선 방어의 기본인 시간은 짧게, 거리는 멀리, 차폐를 고려하여 PET 검사를 수행하는 것이 매우 중요하고, 환자로부터의 공간선량률에 따른 종사자의 개인피폭선량을 줄이기 위한 최선의 노력이 필요하다.

• 한국환경복원기술학회지
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• 제18권3호
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• pp.65-77
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• 2015

The urban park has important functions as a habitat for wildlife as well as open space of rest and community for people. This study was carried out to find what factors of structure and vegetation of urban parks could affect forest bird species diversity in Cheonan city. The study surveyed bird and vegetation species in 26 urban parks, Cheonan city. A correlation analysis and multiple linear regressions were performed to test whether habitat structure and vegetation were the major correlate with species diversity. The results showed the Dujeong park was the most high bird species diversity (H' = 2.13), and the Dujeong-8 park (H' = 2.02) and the Cheongsa park (H' = 1.73) were considerably higher than the other urban parks. The variables that were strongly correlated with bird species diversity were park area, number of subtree species, canopy of shrub, number of shrub species, shape index, canopy of subtree, canopy of tree, and impervious surface ratio. The regression of bird species diversity against the environmental variables showed that 3 variables of park area, canopy of subtree, and canopy of tree were included in the best model. Model variable selection was broadly similar for the 5 optimal models. It means park area and multi-layer vegetation were the most consistent and significant predictor of bird species diversity, because urban parks were isolated by built-up areas. Especially the subtree coverage that provides shelter and food for forest birds was an important variable. Therefore, to make parks circular-shaped and abundant multi-layer vegetation, which could be a buffer to external disturbances and improve the quality of habitats, may be used to enhance species diversity in creation and management of urban parks.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2006년도 춘계학술대회 논문집
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• pp.107-108
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• 2006

This paper shows the design optimization of the paper feeding mechanism under dynamic behavior by using commercial codes of RecurDyn/MTT2D and RecurDyn/AutoDesign which are developed by functionBay, Inc. A virtual mockup for dynamics analysis of the paper feeding mechanism is build on RecurDyn/MTT2D and is simulated. Flexible paper is represented as a series of rigid bars connected by revolute joints and rotational spring dampers. Paper is fed by a contact and friction mechanism on rollers or guides. The slip of the paper and nip force of rollers are measured to estimate the system performance. After a simulation, these performances are automatically send to RecurDyn/AutoDesign which is a sequential approximate optimization tool based on the response surface modeling. RecurDyn/AutoDesign makes the approximate objective function and computes the optimized design points of the design variables and gives them to analysis tool. And then the simulation is repeated with the updated design variables. These processes are repeated until finding a tolerable design optimization. In this paper, a paper feeding mechanism is introduced and it is optimized with the proposed algorithms.

• The Plant Pathology Journal
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• 제20권1호
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• pp.58-62
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• 2004

White root rot of wild white clover (Trifoliorum repens) caused by isolate BWC98-105 has been first reported in Korea. Typical symptoms on root include water-soaked and dark-brown rot, resulting in complete blight of the whole plant. The fungus grew well at $20-28^{\circ}C$ and produced abundant sclerotia at 10-15 days after full mycelial growth on potato dextrose agar. Sclerotia were brown to dark-brown in color and 1-3 mm in length. When white clover plants were inoculated with mycelial suspension ($10^5$ cfu/ml) of isolate BWC98-105, the plant shoots were killed within 4-6 days and the roots were completely blighted. Sclerotia were also formed on the surface of the root covered with whitish mycelia within 10-15 days in the field. All nine isolates developed high incidences of white root rot disease on white clover seedlings, of which the symptoms were similar to those observed in the fields. Hence, their pathogenicity was confirmed on white clover. The infection rate of the fungal isolates varied from 78.5% to 95.2%, among which BWC98-105 was the most virulent isolate. The weeding efficacy of the fungus was maintained until the following year, leading to a significant reduction of reshooting. The fungus was specifically parasitic to white clover, but not to four lawn species including zoysiagrass (Zoysia japonica) under greenhouse test. The fungus also had no response to some Gramineae species including rice, but caused little damage to five species of Leguminosae.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2006년도 춘계학술대회 논문집
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• pp.361-362
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• 2006

This paper presents the robust design of gripper part for a high-speed LCD (Liquid Crystal Display) transfer system. In this paper, the 1st DOE (Design of Experiment) is conducted to find out main-effect factors fur the design of gripper part. Thirty-six experiments are performed using $ANSYS^{(R)}$ and their results are statistically analyzed using $MINITAB^{(R)}$, which shows that the factors, i.e., First-width, Second-width, Rec-width, and thickness of gripper part, are more important than other factors. The main effect plots shows that the maximum deflection and mass of gripper part are minimized by increasing First-width, Second-width, Rec-width and thickness. The 2nd DOE is conducted to obtain RSM (Response Surface Method) equation. The CCD (Central Composite Design) technique with four factors is used. Optimum design is conducted using the RSM equation. Genetic algorithm is used for optimal design. Six sigma robust design is conducted to find out a guideline for control range of design parameter. To obtain six sigma level reliability, the standard deviations of design parameters are shown to be controlled within 5% of average design value.

• 대한용접접합학회:학술대회논문집
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• 대한용접접합학회 2009년 추계학술발표대회
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• pp.21-21
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• 2009

현장의 GMA 용접기는 일반적으로 용접기 본체로부터 와이어 송급장치까지의 거리가 약 50m 정도 떨어져 있고 송급장치로부터 토치까지의 거리는 약 3~5m 정도이다. 특히 국산 GMA 용접기의 와이어 송급장치는 DC 모터의 일종인 프린트모터를 사용하고 있으며 제어기는 오픈루프 제어를 하고 있다. 따라서 와이어 송급속도의 변동을 보상할 수 있는 어떤 수단도 장착되어 있지 않다. 또한 송급장치와 토치간 와이어를 안내하는 케이블 도관의 마찰 때문에 토치 끝단에서의 와이어 송급속도가 불규칙해질 수밖에 없다. 용접 시 와이어 송급속도의 순간적인 변동 때문에 용접부의 전류 파형이 매우 불규칙해지고 이 때문에 용융된 용적이 용융풀로 이행하지 못하고 스패터로 비산하는 현상을 발생시킨다. 본 연구에서는 이를 해결하고자 국산 SCR 용접기 및 인버터 용접기와 호환 가능한 디지털 제어형 와이어 송급장치와 Push-Pull 용접 토치를 개발하였다. 또한, 개발된 시스템을 이용하여 정 역 방향 제어기술을 적용하였고 아크 발생 시의 스패터 최소화 공정을 제안하였다. 실험은 SM490A 강재를 사용하여 BOP(Bead On Plate) 용접을 실행하였다. 용접 중 LabVIEW를 이용하여 아크 발생 초기 전류, 전압 그리고 와이어 송급속도를 측정하였고, 고속 카메라를 이용하여 용접현상을 분석하였다. 본 연구를 통해 아크 발생 초기의 스패터를 최소화하는 공정을 도출할 수 있었다. 용접공정 변수인 아크 발생 초기의 와이어 송급속도, 와이어의 역송급 진행거리 그리고 역송급 판단 시점의 용접 전류 값은 실험을 통해 얻을 수 있었다. 또한, 이 연구를 통해 개발된 시스템의 성능을 평가하였다.