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Phylogenetic position of five Korean strains of Alexandrium tamarense(Dinophyceae), based on internal transcribed spacers ITS1 and ITS2 including nuclear-encoded 5.85 rRNA gene sequences (ITS 부위에 근거한 한국산 Alexandrium tamarense 5 클론의 계통분류학적 위치)

  • Cho, Eun-Seob;Lee, Sam-Geun;Kim, Ik-Soo
    • Journal of Life Science
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    • v.12 no.6
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    • pp.821-834
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    • 2002
  • In order to measure the inter- and intraspecific genetic divergences within the genus Alexandrium, the variations within the internal transcribed spacer (ITS1 and ITS2) regions and 5.85 ribosomal RNA gene of eight Alexandrium species were examined for 33 strains from diverse geographical locations by direct sequencing. Five isolates of A. tamarense (AT-2, AT-6, AT-10, AT-A and AT-B) from Jinhae Bay, Korea were found to be completely identical to a Japanese strain OFX151-A. The length of the amplified ITSI-5.85-ITS2 region varied from 481 nucleotides (in A. margalefi) to 528 nucleotides (in A. affine CU1-1). ITS1 and ITS2 nucleotide lengths were negatively correlated, whereas a positive correlation was found between their G+C content. The degree of sequence divergence ranged from 0.3% (1 bp) to a maximum of 53% (305 Up). Pairwise sequence comparisons revealed a small degree of divergence between A. tamarense and A. Pundyense isolates (1.2 - 2.3% = 6-12 bp), but a high degree of divergence between A. tamarense and A. catenella (19.8% = 102 bp), and between A. catenella and A. Pundyense (19.7%). Although most nodes were weakly supported by bootstrap values, some types tend to form independent molecular groups. A. catenella isolates also formed an independent molecular sub-group, with relaticula strong bootstrap values (94% or 85% and 79% or 98%, respectively in PAUP and NJ trees). Interestingly, A. cohorticula and A. frateculus always clustered within the same sub-group, this result being supported by strong bootstrap values. Our results indicate that the ITS regions provide useful informations on hierarchical population genetic structure and a high phylogenetic resolution in intraspecific and interspecific Alexandrium population.

Singular Value Decomposition based Noise Reduction Technique for Dynamic PET I mage : Preliminary study (특이값 분해 기반 Dynamic PET 영상의 노이즈 제거 기법 : 예비 연구)

  • Pyeon, Do-Yeong;Kim, Jung-Su;Baek, Cheol-Ha;Jung, Young-Jin
    • Journal of radiological science and technology
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    • v.39 no.2
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    • pp.227-236
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    • 2016
  • Dynamic positron emission tomography(dPET) is widely used medical imaging modality that can provide both physiological and functional neuro-image for diagnosing various brain disease. However, dPET images have low spatial-resolution and high noise level during spatio-temporal analysis (three-dimensional spatial information + one-dimensional time information), there by limiting clinical utilization. In order to overcome these issues for the spatio-temporal analysis, a novel computational technique was introduced in this paper. The computational technique based on singular value decomposition classifies multiple independent components. Signal components can be distinguished from the classified independent components. The results show that signal to noise ratio was improved up to 30% compared with the original images. We believe that the proposed computational technique in dPET can be useful tool for various clinical / research applications.

Mendelian Inheritance of Inter-Simple Sequence Repeats Markers in Abies Koreans Wilson (구상나무에 있어서 Inter-Simple Sequence Repeats Marker의 유전양식(遺傳樣式))

  • Hong, Yong-Pyo;Cho, Kyung-Jin;Kim, Yong-Yul;Shin, Eun-Kyeong
    • Journal of Korean Society of Forest Science
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    • v.87 no.3
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    • pp.422-428
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    • 1998
  • Polymerase chain reaction(PCR)-based inter-simple sequence repeats(I-SSR) markers were analyzed in 48 megagametophytes of a single tree of Abies koreana $W_{ILS}$. Nineteen of the 35 primers, screened with 6 megagametophyte DNA and produced the clearest amplification products in the preliminary experiment, were used for PCR with 48 megagametophyte DNAs sampled from a single tree. On the basis of the chi-square test, a total of 51 amplicons, amplified by the 19 primers, were revealed to be segregated according to the Mendelian ratio(i.e., 1 : 1 segregation ratio) in the 48 megagametophytes at 5% significance level. Based on the linkage analysis, the observed 51 Mendelian loci turned out to be unlinked each other, which suggested that they are evenly distributed in the genome. However, majority of RAPD markers are known to belong to the independent linkage blocks, which frequently results in the amplification of RAPD markers from the restricted regions of the genome. Owing to the nature of even distribution of the 51 loci observed in this study, the I-SSR markers could give better resolution of estimating genetic diversity from the whole genome than RAPD markers. And I-SSR markers are also more suitable than RAPD markers for reconstructing phylogenetic relationship by a cladistic method which requires to fulfil the assumption of independent evolution of the different characters.

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PRISM-KNU Development and Monthly Precipitation Mapping in South Korea (PRISM-KNU의 개발과 남한 월강수량 분포도 작성)

  • PARK, Jong-Chul;KIM, Man-Kyu
    • Journal of the Korean Association of Geographic Information Studies
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    • v.19 no.2
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    • pp.27-46
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    • 2016
  • In this study, the parameter-elevation regressions on independent slopes model-Kongju National University(PRISM-KNU) system was developed to interpolate monthly precipitation data. One of the features of PRISM-KNU is that it can adjust the allowable range of slope according to the elevation range in the equation representing a linear relationship between the precipitation and elevation. The parameter value of the model was determined by using the optimization technique, and the result was applied to produce monthly precipitation data with a spatial resolution of $1{\times}1km$ from 2000 to 2014 in South Korea. In the result, the Kling-Gupta Efficiency for model evaluation was over 0.7 in 86% of the total cases simulated. In addition, a dramatic change in the spatial pattern of precipitation data was observed in the output of the Modified Korean PRISM, but such a phenomenon did not occur in the output of the PRISM-KNU. This study confirmed the appropriateness of the PRISM-KNU, and the result showed that the spatial consistency of the data produced by the model improved compared with that produced by the Modified Korean PRISM. It is expected that the PRISM-KNU and its output will be utilized in various studies in the future.

Legal Issues on the Franchise Disputes and their Settlement by Arbitration (가맹계약분쟁과 중재에 관한 법적 문제)

  • Choi, Young-Hong
    • Journal of Arbitration Studies
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    • v.17 no.1
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    • pp.57-75
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    • 2007
  • Ever since franchising emerged in the industry of distribution, it has been growing explosively in the U.S.A. and all other countries as well. It is a method of expanding a business by licensing independent businessman to sell the franchiser's products and/or services or to follow a format and trade style created by the franchiser using the franchiser's trade marks and trade names. Franchising is a form of business that touches upon many different areas of law including, but not limited to, general contract law, general principles of commercial law, law of intellectual property, competition law, fair trade practices law and other industry specific laws e.g., the Fair Practices in Franchising Act in Korea. Arbitration is a long established, legally recognized procedure for submitting disputes to an outside person(s), mutually selected by the parties, for a final and binding decision. Despite its merits as an alternative dispute resolution, it has been criticized, on the other hand, particularly by franchisees' attorneys on the ground that even though it is required to protect the franchisees against the enforcement of pre-dispute arbitration agreements because of the franchisees' paucity of bargaining power vis-a-vis the franchiser, arbitration cannot afford it. Until recently, however, little has been written about the legal issues pertaining to franchise agreement and arbitration clause contained therein in Korea. This treatise reviews the cases and arguments in relation to the subject especially of the U.S.A., which have been accumulated for decades. The issues addressed herein are the pre-emption by the FAA, the disputes to be arbitrated, the selection and qualification of arbitrators, the place of arbitration hearings and the evidentiary rules applicable, the expenses of arbitration, theory of fiduciary duty and the like, all of which are relevant to franchise agreement.

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Statistical estimation of crop yields for the Midwestern United States using satellite images, climate datasets, and soil property maps

  • Kim, Nari;Cho, Jaeil;Hong, Sungwook;Ha, Kyung-Ja;Shibasaki, Ryosuke;Lee, Yang-Won
    • Korean Journal of Remote Sensing
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    • v.32 no.4
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    • pp.383-401
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    • 2016
  • In this paper, we described the statistical modeling of crop yields using satellite images, climatic datasets, soil property maps, and fertilizer data for the Midwestern United States during 2001-2012. Satellite images were obtained from the Moderate Resolution Imaging Spectroradiometer (MODIS), and climatic datasets were provided by the Parameter-elevation Regressions on Independent Slopes Model (PRISM) Climate Group. Soil property maps were derived from the Harmonized World Soil Database (HWSD). Our multivariate regression models produced quite good prediction accuracies, with differences of approximately 8-15% from the governmental statistics of corn and soybean yields. The unfavorable conditions of climate and vegetation in 2012 could have resulted in a decrease in yields according to the regression models, but the actual yields were greater than predicted. It can be interpreted that factors other than climate, vegetation, soil, and fertilizer may be involved in the negative biases. Also, we found that soybean yield was more affected by minimum temperature conditions while corn yield was more associated with photosynthetic activities. These two crops can have different potential impacts regarding climate change, and it is important to quantify the degree of the crop sensitivities to climatic variations to help adaptation by humans. Considering the yield decreases during the drought event, we can assume that climatic effect may be stronger than human adaptive capacity. Thus, further studies are demanded particularly by enhancing the data regarding human activities such as tillage, fertilization, irrigation, and comprehensive agricultural technologies.

Association of carotid atherosclerosis and obesity in type 2 diabetic patients (제2형 당뇨병 환자에서 비만과 경동맥 경화증과의 관계)

  • Gang, Se-Hun;Kim, Gyeong-Min;Jo, Dong-Hyeok;Gang, Ho-Cheol;Jeong, Dong-Jin;Jeong, Min-Yeong
    • Journal of Korea Association of Health Promotion
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    • v.4 no.1
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    • pp.12-27
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    • 2006
  • "본 논문은 대한내과학회지 2006년 제70권 제3호에 실렸던 논문으로 대한내과학회 편집위원회의 승인을 득하고 본 협회지에 게재함. Background : Diabetes mellitus is a major independent risk factor for atherosclerosis. In recent years non-invasive high resolution B-mode ultrasound methods have been developed to measure the intima-media thickness(IMT) of the carotid artery as an indicator for early atherosclerosis. Itis known that obesity plays a role in the development of type 2 diabetes and cardiovascular disease, and it has also been reported that not only the amount but also the distribution of body fat is important. This study investigated the relationship between obesity and the development of carotid atherosclerosis in type 2 diabetic patients. Methods: Carotid IMT was measured by ultrasound B-mode imaging in 144 patients with type 2diabetes mellitus. All subjects underwent assessment for the degree and distribution of obesity, the presence of coronary artery disease risk factors, and the presence of diabetic complications. Resuts: Carotid IMT was increased in the abdominal obese group defined by waist circurference. However, there was no significant difference in carotid TMT between the non-obese group and obese group as defined by body mass index, waist to hip ratio, and total body fat percent measured by bio electrical impedance analysis. There were positive correlations between carotid IMT and age, duration of diabetes, systolic blood pressure, and waist circumference. Multiple linear regression analysis revealed the variable that interacted independently with carotid IMT was age in type 2 diabetic patients. Carotid IMT was significantly increased in type 2 diabetic patients with macrovascular complications and microvascvlar complications .Conclusion: This study suggested that abdominal obesity rather than general obesity was associated with carotid atherosclerosis reflected by increment of carotid IMT in type 2 diabetic subjects.

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Evidence for the Luminosity Evolution of Type Ia Supernovae from the Ages of Early-type Host Galaxies

  • Lee, Young-Wook;Kang, Yijung;Kim, Young-Lo;Lim, Dongwook;Chung, Chul
    • The Bulletin of The Korean Astronomical Society
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    • v.38 no.2
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    • pp.56.1-56.1
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    • 2013
  • Supernovae type Ia (SNe Ia) cosmology is providing the only direct evidence for the presence of dark energy. This result is based on the assumption that the look-back time evolution of SNe Ia luminosity, after light-curve shape correction, would be negligible. However, the most recent compilation of SNe Ia data shows systematic difference in the Hubble residual (HR) between the E and Sd/Irr galaxies, indicating that the light-curve fitters used by the SNe Ia community cannot quite correct for a large portion of the population age effect. In order to investigate this possibility more directly, we have obtained low-resolution spectra for 30 nearby early-type host galaxies. This data set is used to estimate the luminosity-weighted mean ages and metallicities of host galaxies by employing the population synthesis models. We found an interesting trend between the host galaxy age and HR, in the sense that younger galaxies have positive residuals (i.e., light-curve corrected SNe Ia luminosity is fainter). This result is rather independent of the choice of the population synthesis models employed. Taken at face value, this age (evolution) effect can mimic a large fraction of the HR used in the discovery of the dark energy. This result is significant at 1.4 - 3 sigma levels, depending on the light curve fitters adopted, and further observations and analyses are certainly required to confirm the trend reported here.

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A Study on The Needs in The Family Life Education Among The Middle Aged Employed Wives and House Wives (중년기 주부의 취업여부에 따른 가족생활교육 요구도 분석)

  • 김명자
    • Journal of Families and Better Life
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    • v.15 no.1
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    • pp.95-110
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    • 1997
  • This study is to find out whether the family life education can help the middle aged wives to resolve their problems and to keep their lives healthy. For this purpose self-administered questionnaires were used for 628 persons whose ages between 40 to 59 residing in the Metropolitan Area of Soul. Deta collected were analyzed by statistical methods such as the factor analysis frequency distribution percentile mean t-test chi-square Anova and Duncan's multiple range test through the SAS program package. Major results obtained are as follows; 1) The most demanding education content in the are of the spousal relationship was the helping program for the role change from parent-child relationship to spousal relationship. Employed wives demanded conflict resolution skill training program between parents and children while house wives showed the highest interest in guiding their children's future. 2) There are significant differences between employed wives and house wives in the areas f parent-child relationship communication and home managment of the need assessment. In detail employed wives showed higher interest in education the children more independent than house wives. Employed wives also showed higher interest than house wives in the education content about the factors for communication problems in the communication area. 3) About the application method in the family life education there was no significant difference between employed wives and house wives in terms of the necessity of the problem solving methods of follow-up meeting in the family life education while there was a significant difference in the past education program participation intention to participate in the future education program and the education setting between employed wives and house wives.

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Development of Digital Radiography System Using by an One Dimensional MWPC (1차원 MWPC를 이용한 디지탈 X-선 사진촬영장치의 개발)

  • Park, Jung-Byung;Moon, Myung-Kook;Goo, Sung-Mo;Cho, Jin-Ho;Kim, Do-Sung;Kang, Hee-Dong
    • Journal of Sensor Science and Technology
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    • v.4 no.4
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    • pp.62-69
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    • 1995
  • We have developed the digital radiography system applied by the one dimensional multiwire proportional chamber. X-ray position signals were obtained from anode wires which were connected to counters through amplifiers and discriminators. The chamber was made of gas flow type and detector gas was P10. The threshold voltage which gives to the discriminator is independent on the neighboring channels. This improved the uniformity of the detector. Then the differential nonlineality is ${\pm}4%$. Increasing the gas pressure, the spatial resolution is about 1.4-mm at which the pitch of the anode wire is 2-mm. The object is scanned in vertical direction to take an image. The number of pixels in the image is $32{\times}32$.

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