• Korean Journal of Food Science and Technology
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• v.32 no.3
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• pp.618-628
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• 2000

The indigestible dextrin I was prepared by hydrolyzing pyrodextrin with thermostable ${\alpha}-amylase$. The mean values of indigestible fraction and dieatry fiber of indigestible dextrin I prepared from yellow dextrin were 50.0% and 25.0%, respectively. Also the indigestible dextrin II was prepared by removing low molecular weight saccharides containing glucose with ethanol from enzyme hydrolysate of pyrodextrins. Over 80% of glucose and maltose in initial enzyme hydrolysate were removed, therefore the indigestible fraction and dietary fiber of the indigestible dextrins increased. The indigestible dextrin from ethanol precipitate of enzyme hydrolysate of yellow dextrin by ${\alpha}-amylase$ and amyloglucosidase showed a higher contents of indigestible fraction and dietary fiber than ethanol precipitates by any other enzyme combination, and its mean values were 83.6% and 62.8%, respectively. Consequently, it was found that the indigestible dextrins which are resistant to starch-hydrolysing enzyme can be easily prepared from pyrodextrin, and presumed that they can perform physiological functions as soluble dietary fiber.

• The Korean Journal of Ecology
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• v.21 no.5_2
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• pp.575-583
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• 1998

Physicochemical factors, microbial population size and the properties of the bacterial isolates were assessed to find out the nature of soil ecosystem of Mt. Paektu. Samples were obtained from the surface layer of soils on which specific plant community is developed. Average content of moisture, organic matter and avaiable phosphate of the soils were 21.6%, 17.3% and 2.48mg/100g, respectively. These values were similar to those of developing forest soils, but were slightly lower than those of climax ecosystem such as Piagol in Mt. Chiri. The population size of soil bacteria ranged from 2.7 to $202.5{\times}10^5$ CFU/g.dry soil, and the size is somewhat dependent on the content of moisture and oranic matter of the forest soil. A large number of bacteria was able to decompose macromolecules such as starch, elastin and gelatin. While the distribution rate of resistant bacteria to antibiotics was high, that to toxic chemicals was low. This means that the competition between microorgani는 predominate over the interference with artificial behaviour such as spread of pesticides in the surveyed region. Bacterial species composition of each soil was comparatively simple. Pseudomonas, Agrobacterium, Flavobacterium and Xanthomonas which are Gram-negative short rods were widely distributed in the forest soils. The endospore forming Bacillus species were also main constituents of the soil microflroa. any one of the strains was not identified as Azospirillum or Micrococcus which are known to be one of major constituents of the forest soil. for the correct identification of isolates chemotaxonomic studies will be proceeded, and the strains are to be stored in the Type collection Center.

• Journal of the Korean Society of Food Culture
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• v.20 no.3
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• pp.331-336
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• 2005

Pysicochemical and sensory characteristics of health concerned funtional cookies using various levels of crude ingredients of resistant starch were investigated in this study. The 10% substituted group had significantly higher pH values compared to those of control. Spread factor of control group had significantly the largest value and the 30% substituted group had the lowest values(p<0.05). Results of sensory characteristics showed significantly higher hardness, crispness and yellowness with 20% substituted sample group at p<0.05. Acceptance test of cookies with 10% substituted sample groups showed higher savory taste, texture, and overall acceptability and they did not show significant differences with the 20% substituted groups.

• Analytical Science and Technology
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• v.35 no.4
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• pp.161-168
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• 2022

Wheat is rich in sources of fiber, oligosaccharides, and resistant starch, simple carbohydrates which may have a protective role against carcinoma. Additionally, Whole wheat/bran as well includes contains phytochemicals such as flavonoids, lignans, folate, phytosterols, phenolic acids, and tocols. The above phytochemicals suitable forms antioxidant and cholesterol-reducing activities. Phytoestrogens are regarded as especially essential in the preventative measures of hormonally dependent malignancies such as breast cancer (BC). In this study lowered BC risk has been associated with whole grain/bran consumption with an odds ratio (OR=0.24 and 95 %CI=0.10-0.56). Wheat/bran appears to have a reliable protective impact against BC. While intake of white bread has been associated with a high risk of BC (OR=2.63 and CI 95 %=1.07-6.48). Also, the E-Selectin (SELE) Single nucleotide polymorphisms (SNPs) rs5353 A/G and rs932307 C/T were investigated using the sanger sequences approach. There was a positive association between genotypes (rs5353 GG+AG) and (rs932307 TT+CT) with rate consumption of wheat-white/bread and these genotypes were more frequent in patients had BC in comparison with a significant difference (P=0.03) (P=0.01), respectively. The genotypes (GG+AG) frequency of the rs5353 polymorphism and (TT+CT) genotypes of the rs932307 polymorphism in the present study had a high risk of cancer with (OR=3.05), (OR=4.17) respectively. While these genotypes showed no significant association with the rate of whole grain consumption in patients and control. Therefore, the type of wheat may associate with increased incidence of disease-related with type of Polymorphism because some present genotypes of SNPs showed high (OR) which may refer to their positive associated with disease, the white wheat consumption may active the risky association between SNPs and BC.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.674-678
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• 2010

A new sweetpotato variety, 'Daeyumi', was developed by Bioenergy Crop Research Center, National Institute of Crop Science (NICS), RDA in 2008. This variety was obtained from the cross between 'Jinhongmi' and 'Xusju 18' in 2000. The seedling and line selections were performed from 2001 to 2003, preliminary and advanced yield trials were carried out from 2004 to 2005, and the regional yield trials were conducted at six locations from 2006 to 2008. 'Daeyumi' has cordate leaf, green vine and petiole, elliptic storage root, red skin and yellow flesh color of storage root. This variety is also resistant to Fusarium wilt and nematode. The starch value was 25.9%, ethanol yield was 418 L/Ton, which was 7% higher than that of 'Yulmi' variety, and the total sugar content was 2.47 g/100g, dry weight. 'Daeyumi's initial temperature of starch gelatinization was lower, 76.2$^{\circ}C$, and the retrogradation process was earlier than 'Yulmi'. The average yield of storage root was 27.8 ton/ha in the regional yield trials, which was 36% higher than that of 'Yulmi' variety. Number of storage roots over 50 gram per plant was 3.0, and the average weight of one storage root was 152 gram. This variety can be used for the production of bioethanol and starch processing.

• Korean journal of applied entomology
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• v.39 no.3
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• pp.157-163
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• 2000

In a previous report, 50 potato cultivars were screened for infestation levels by the coppery click beetle (Selatosomus puncticollis Motschulsky) in the field. Subsequently, we selected 10 potato cultivars (Anco, Atlantic, Bintje, Dejima, Denali, Jopung, Irish Cobbler, Namsuh, Shepody, and Superior) to evaluate feeding preferences by wireworms, and to analyze some factors associated with resistance. The injury rates and number of holes in potato tubers damaged by larvae of S. puncticollis were checked in the field and laboratory. Additionally, some of their chemical characters (contents of glycoalkaloids, total-nitrogen, Ca, K, Mg, sugars, and starch) were quantified. And finally correlation analysis was conducted to see whether there is a possible relationship between these characteristics and the damage level. The tuber injury rates by S. puncticollis larvae were generally high showing 19% to 73% of damage level. The highest number of tuber hole damaged by S. puncticollis larvae was found on cv. Namsuh, but generally fewer on cvs. Anco, Atlantic, Bintje, Denali and Superior. No activities for $\alpha$-chaconine, $\alpha$-solanine and $\alpha$-tomatine at a concentration of 2,500 ppm were found to S. puncticollis larvae. The contents of glycoalkaloids in tuber were different depending on cultivars. In tubers, cv. Superior contained the highest level of 18.8 mg%, but cv. Irish Cobbler had the lowest level of 6.39 mg%. Concentrations of reducing sugars and total free sugars in tubers of cv. Namsuh were 0.71 % and 2.95%, but 0.26% and 1.77% in those of cv. Dejima, respectively. For the content of potato starch, cvs. Bintje, Dejima and Irish Cobbler showed higher level, but cvs. Jopung and Shepody lower. The highest contents of total nitrogen, Ca, K and Mg in tuber were found on cvs. Anco, Atlantic, Anco and Jopung, respectively. From correlation analyses, injury rate by S. puncticollis larvae was correlated with total nitrogen content (r = -0.71435*) and total sugar content in tuber (r = 0.78018*). Such information will become essential in developing integrated pest management programs and also in breeding new potato cultivars resistant to the wireworms.

• Journal of Plant Biotechnology
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• v.40 no.1
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• pp.18-26
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• 2013

Genetically modified (GM) rice Agb0101, which expresses the insecticidal toxin modified cry1Ac (mcry1Ac1) gene, was developed by the Rural Development Administration in Korea. To monitor the probable release of Agb0101 in the future, it is necessary to develop a reliable detection method. Here, we developed the PCR detection method for monitoring and tracing of GM rice. The primer pair (RBEgh-1/-2) from a starch branching enzyme (RBE4) gene was designed as an endogenous reference, giving rise to an expected PCR amplicon of 101 bp. For the qualitative PCR detection, construct- and event-specific primers were designed on the basis of integration sequence of T-DNA. Event-specific PCRs amplified specifically 5'- or 3'-junction region spanning the native genome DNA and the integrated gene construct, while none of amplified product was shown on crops, rice varieties, and other insect-resistant transgenic rice lines. The event-specific real-time PCR method was performed using TaqMan probe and plasmid pRBECrR containing both rice endogenous gene RBE4 sequence and 5'-junction sequence as the reference molecule. The absolute limit of quantification (LOQ) of real-time PCR was established with around 10 copies for one plasmid molecule pRBECrR. Thereafter, the different amounts of transgenic rice (1, 3, 5, and 10%, respectively) were quantified by using the established real-time PCR method, with a range below 19.55% of the accuracy expressed as bias, 0.06-0.40 of standard deviation (SD) and 3.80-7.01% of relative standard deviations (RSD), respectively. These results indicate that the qualitative and quantitative PCR methods could be used effectively to detect the event Agb0101 in monitoring and traceability.

• Korean Journal of Plant Resources
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• v.8 no.2
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• pp.189-193
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• 1995

A new paeony variety 'Euiseongjakyak' was developed by Gyeongbug Provincial Rural Development Administration in 1993. This variety was selected from pure lint selection. It is characterized by red flower of single type, deep white flesh colour, straight and big radixs. When compared with 'Punggi jerye' a check variety, it is more resistant to lodging, disease and insect injury, and outyielded by 32% in total radix and 17% in marketable radix during 3 years regional performance trials. In the radix, the content of starch and sugar was higher by $9.8\sim12.22%$, crude protein and ash was similar and fiber was lower by 1.17% compared to 'Punggi jerye'.

• Proceedings of the Korean Journal of Food and Nutrition Conference
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• 2001.12a
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• pp.124-124
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• 2001

난소화성전분(resistant starch, RS)는 건강한 사람의 소장에서 흡수되지 않는 전분과 분해된 전분의 산물을 총칭하는데. 대장에서 발효와 미생물성장, 식후 혈당, 변부피, 장통과시간, 식품의 열량가 등에 영향을 미치므로 생리적인 이점을 갖는다. 본 연구에서는 고지방식이와 콜레스테롤을 섭취란 흰쥐에서 제조과정이 다른 RS의 섭취가 저 열량원으로서 체중감소나 혈액내 지방성분을 낮추는 효과가 있는지를 알아보는 것을 목적으로 하였다. 연구방법으로 실험동물은 생후 4주된 Sprague-Dawl ey계 수컷 흰쥐로, 지방을 식이 무게의 20%, 콜레스테롤이 식이 무게의 1%로 첨가 된 고지방식이로 6주를 사육시켜 비만과 고콜레스테롤증을 유발시켰다. 고콜레스테롤이 유발된 실험동물은 섭취하는 전분의 형태가 다른 실험식이로 4주간을 더 사육하였고 사육기간이 끝난 실험동물들은 희생하여 생화학적인 실험- 혈액과 간의 총지방양, 중성지방양, 콜레스테롤양을 측정하였고, 면역능력으로 비장세포 증식, 혈장내 IgG, $C_3$양을 측정하였고. 혈장내 leptin 양을 측정하였다. 실험결과는 체중과 체내 저장지방무게가 난소화성전분 혼합군(RSNE)에서 유의적으로 감소하였다. 혈장내 지방성분을 보면, 총 지방의 경우 고지방대조군(HC)이 저지방 대조군(LC)에 비래 유의적으로 높았으며, RS를 섭취하면 유의적으로 낮아지는 경향을 보여 저지방을 섭취한 LC군에 비해서 낮은 수치를 나타내고 있다. 혈장 중성지방은 저지방 즉 고 탄수화물식이를 섭취하는 LC군이 유의적으로 높았으며 고지방을 섭취하는 경우는 실험군에 차이가 없었다. 혈장 총 콜레스테롤은 LC군에 비해 고지방과 콜레스테롤을 섭취하는 HC군이 유의적으로 높았으며. RSNE군에서는 유의적인 감소를 보였다. HDL-콜레스테롤에서는 실험군간에 차이가 없었다. 간내 총지방과 콜레스테롤량, 중성지방량은 LC군이 다른 군에 비해 유의적으로 적었으며 고지방섭취군들은 섭취하는 전분의 종류에 따른 차이가 나타나지 않았다. 면역능력을담당하는 체내 기관인 비장세포의 중식능력은 실험군간에 유의적인 차이가 없으나 혈장의 Ig G 양은 RSNE군이 유의적으로 높고, $C_3$ 양은 난소화성 전분 추출군(RSEX)에서 유의적으로 높게 나타났다. 혈장에서의 leptin 농도는 고지방 대조군인 HC군이 유의적으로 높았으며, RSNE군이 LC군과 같이 유의적으로 낮았다. 결론으로 난소화성 전분(RS)를 식이무게의 10%로 첨가하는 수준에서 순수한 RS보다는 RS제조과정 중 순수한 RS를 분리하기 전 단계인 RS를 포함한 전분이 체중감소, 체내 저장지방감소, 혈액 내 콜레스테롤을 저하시키는 효과를 보였다. 그러므로 RS의 섭취는 섭취형태에 따라 저 열량원으로, 혈액 내 지방성분을 낮추는 효과를 가진 기능성 식품소재로 사용될 수 있을 것이다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.4
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• pp.285-292
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• 2017

We investigated physicochemical properties of puffed snacks with intermediate and high amylose rice varieties. The intermediate amylose rice varieties 'Sindongjin' and high amylose rice varieties newly developed for food processing, 'Dodamssal' and 'Goami4' were tested for this study. The crude fat and crude protein contents of the rice cultivars ranged 1.47-3.08% and 6.30-7.63%, respectively. The resistant starch and amylose contents of Dodamssal and Goami4 were higher than those of Sindongjin. The hardness of rice was the highest in Sindongjin and Dodamssal. Also, Hardness of puffed snacks decreased by 72.07% for Sindongjin, 88.21% for Dodamssal and 66.67% for Goami4 compared to raw rice samples. The sensory evaluation showed that the highest scores in taste, texture and overall acceptability of puffed snacks were obtained in Dodamssal. The results of this study indicate that Dodamssal was suitable varieties for puffed snacks. Also, the physicochemical properties of Dodamssal were improved by the extrusion process. Therefore Dodamssal can be used for the industrial production of puffed snacks.