• 대한임상검사과학회지
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• 제41권1호
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• pp.24-30
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• 2009

Rifampicin (RIF) and isoniazid (INH) are the most important drug for the treatment of Mycobacterium tuberculosis. Mutations correlated to rifampicin and isoniazid-resistance have been detected in rpoB gene and katG gene, respectively. Of the rifampicin-resistant isolates, 90% showed mutations in rpoB gene at codon 507 to 533. Isoniazid-resistant isolates analysed had a mutation in katG at codon 315. The aim of this study is to develop a pyrosequencing-based approach for rapid detection of ripampin or isoniazid resistant M. tuberculosis based on characterization of all possible mutation in the target region. For this study, the DNA selected from 35 cases of MTB PCR positive clinical sample such as bronchial washing, sputum, and pleural fluid. RIF or INH resistant was analyzed by pyrosequencing data of rpoB and katG gene. 28 (80%) and 7 (20%) of 35 MTB PCR positive DNAs were occured rifampicin-sensitivity and resistant, respectively. For INH, 30 (85.7%) and 5 (14.5%) cases were detected isoniazid-sensitivity and resistant, respectively. When pyrosequencing analysis was compared with ABI sequencing analysis, both analysis were presented same result, but pyrosequencing analysis was more rapid than ABI sequencing analysis. In conclusion, we found that pyrosequencing technology offers high accuracy, specificity, short turn around time and a high throughput in detection of rifampicin or isoniazid resistance in M. tuberculosis.

• 대한의생명과학회지
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• 제5권1호
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• pp.95-100
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• 1999

일반적으로 임상검사실에서 vancomycin resistant enterococci (VRE)를 검출하는 일은 어렵고, 시간이 많이 들며, 검체처리 비용도 많이 든다. 따라서 본 실험은 임상검체에서 분리된 세균으로부터 VRE를 신속하게 확인하고, 진단하기 위한 방법으로서 다중 중합효소 연쇄반응을 확립하였다. 본 실험에 사용된 primer는 장구균에 특이 한 유전자인 vanA, vanB, vanC-1, vanC-2/3 각각의 염기서열을 기초로 primer를 제작하고, 다중 중합효소 연쇄 반응을 실시하여 임상검체로부터 분리된 VRE 유전자의 type및 분포율을 조사하고자 하였다. 국내에서 분리된 75주의 장구균을 대상으로 다중 중합효소 연쇄반응을 실시한 결과 36주의 분리균주에서 vancomycin에 대해 높은 저항성을 보이는 vanA 유전자를 가진 것으로 나타났다. 그리고 18주에서는 vancomycin에 낮은 저항성을 내성을 보이는 vanC-1 또는 vanC-2/3 유전자를 보유한 것으로 나타났다. 따라서 본 실험에서 확립한 다중 중합효소 연쇄 반응 기법은 신속한 VRE 진단 방법으로 이용할 수 있을 것이다.

• 센서학회지
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• 제26권5호
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• pp.306-313
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• 2017

An early detection of infiltration in veins is essential to minimize the injuries caused during infusion therapy, which is one of the most important tasks for nurses in clinical settings. We report that bioelectrical impedance analysis is useful in the early detection of infiltration at puncture sites. When infiltration was intentionally induced in the vein of a rabbit's ear, impedance parameters showed significant difference before and after infiltration. In particular, the relative resistance at 20 kHz in the vein of rabbit's ear reduced largely at infiltration, decreased slowly, and then stayed at a constant value. This indicates that the vein in the ear of the rabbit is small, and hence the infiltrated intravenous (IV) solution no longer accumulates after 3 minutes of infiltration. However, when infiltration was induced in the vein of a human's forearm, the relative resistance at 20 kHz decreased gradually over time. In the $R-X_c$ graph, the positions in infiltration induced in the rabbit's ear rapidly shifted before and after infiltration whereas the positions in infiltration induced in the human's forearm changed gradually during infiltration. Our findings suggest that bioelectrical impedance analysis is an effective method to detect the infiltration early in a noninvasive and quantitative manners.

• 비파괴검사학회지
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• 제36권6호
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• pp.483-489
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• 2016

강자성 배관의 대표적인 비파괴검사 방법으로 접촉방식인 초음파탐상(UT)과 비접촉식 검사인 누설자속탐상(MFL), 전자기초음파탐상(EMAT), 원격장 와전류탐상(RFECT) 기법 등이 있다. 특히 원격장 와전류(RFECT) 기법은 배관의 직경보다 작은 시스템 구축 등의 장점이 있다. 이런 장점에도 불구하고 array system을 구성할 경우 coil sensor 각각의 민감도 차이와 유지 보수 등의 문제가 있다. 이런 문제점을 해결하기 위해 크기가 작고 교체성이 우수하며 같은 민감도를 갖는 GMR sensor(giant magneto-resistance)를 적용하였다. 본 연구는 강자성 배관에 GMR sensor의 축 및 반경 방향의 원격장 및 깊이 변화를 가진 표준결함 실험을 통해 원격장 및 결함신호 특성을 확인하였고 강자성 배관에 원격장 와전류를 이용한 GMR sensor의 적용 가능성을 확인하였다.

• 한국구조물진단유지관리공학회 논문집
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• 제27권5호
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• pp.67-74
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• 2023

세계적으로 많은 수의 콘크리트 구조물이 건설되어 사용되고 있다. 구조물을 안전하고 지속적으로 사용하기 위해서는 꾸준한 점검과 유지관리가 필요하다. 인력을 활용한 점검과 유지관리 기법은 효율적이긴 하지만, 현장 점검이 이루어지는 시점에 대한 단속적인 상태에 대한 점검만 가능하다. 이에 구조물의 상태를 연속적으로 모니터링 할 수 있는 시스템의 필요가 높아지고 있다. 따라서, 콘크리트 구조물의 표면에 전도성도막을 설치하여 균열 및 손상의 탐지하는 연구를 수행하였다. 콘크리트 구조물의 구조적 특성을 반영하여 균열의 발생과 진전을 모니터링 할 수 있는 병렬저항 형식의 균열감지패턴을 개발하였고, 이에 대한 실증연구를 수행하였다. 철근콘크리트 보부재를 제작하여 균열감지패턴을 설치하였고, 하중가력실험을 통해 균열 모니터링의 가능성을 검증하였다.

• Journal of Microbiology and Biotechnology
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• 제24권3호
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• pp.427-430
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• 2014

We developed a novel immunochromatographic assay (ICA) (EZ-Step VanA rapid kit; Dinona, Korea) for the detection of VanA ligase from vancomycin-resistant enterococci (VRE). Of eight monoclonal antibodies screened by ELISAs, the VanA ligase ICA constructed with 1H9 plus 3G11 showed the greatest reactivity. The detection limit of the kit was $6.3{\times}10^6$ CFU per test. Of 127 vancomycin-resistant microorganisms, 100 vanA VRE were positive in the VanA ligase ICA, and 27 non-vanA vancomycin-resistant isolates were negative. These results were consistent with those of the PCR analyses. Thus, our ICA is a reliable and easy-to-use immunological assay for detecting VanA-producing VRE in clinical laboratories.

• 로봇학회논문지
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• 제17권2호
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• pp.172-177
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• 2022

In general, detection robots using ultrasonic sensors are equipped with sensors to protrude outward or to contact objects. However, in the case of a screw-propelled robot that detects the inside of a reactor tendon duct, if the ultrasonic sensor protrudes to the outside, resistance due to grease is generated, and thus the propulsion efficiency is reduced. In order to increase the propulsion efficiency, the screw must be sharp, and the sharper the screw, the more difficult it is to apply a high-performance ultrasonic sensor, and the detection efficiency decreases. This paper proposes a screw shape-changing mechanism that can improve both propulsion efficiency and detection efficiency. This mechanism includes an overlapped helical ring (OHR) structure and a magnetic clutch system (MCS), and thus the shape of a screw may be changed to a compact size. As a result, the Screw-propelled robot with this mechanism can reduce the overall length by about 150 mm and change the shape of the screw faster and more accurately than a robot with a linear actuator.

• 대한기계학회논문집A
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• 제22권3호
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• pp.644-653
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• 1998

In resistance spot weld, the assurance of weld quality has been a long-standing problem. Since the weld nuggets if resustance spot welding form between the workpieces, visual detection of defects in usually impossible. Welding quality of resistance spot welding can be verified by non destructive and destructive inspections such as X-Ray inspection and testing of weld strength. But these tests, in addition to being time-consuming and costly, can entail risks due to sampling basis. The purpose of this study is the development of the monitoring system based on fuzzy inference, aimed at diagonosis of quality in resistance spot welding. The fuzzy inference system consists of fuzzy input variables, fuzzy membership functions and fuzzy rules. For inferring the welding quality(strength), the experimental data of the spot welding were acquired in various welding conditions with the monitoring system designed. Some fuzzy input variables-maximum, slop and difference values of electrode movement signals-were extracted from the experimental data. It was confirmed that the fuzzy inference values of strength have a .${\pm}$5% error in comparison with actual values for the selected welding conditions(9-10.5KA, 10-14 cycle, 250-300 $kg_f$). This monitoring system can be useful in improving the quality assurance and reliability of the resistance spot welding process.

• Journal of Microbiology and Biotechnology
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• 제25권7호
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• pp.1154-1162
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• 2015

The emergence of carbapenem resistance among Pseudomonas aeruginosa is an increasing problem in many parts of the world. In particular, metallo-$\beta$-lactamases (MBLs) and AmpC $\beta$lactamases are responsible for high-level resistance to carbapenem and cephalosporin. We studied the diversity and frequency of $\beta$-lactamases and characterized chromosomal AmpC $\beta$lactamase from carbapenem-resistant P. aeruginosa isolates. Sixty-one carbapenem-resistant P. aeruginosa isolates were collected from patients in a tertiary hospital in Daejeon, Korea, from January 2011 to June 2014. Minimum inhibitory concentrations (MICs) of four antimicrobial agents were determined using the agar-dilution method. Polymerase chain reaction and sequencing were used to identify the various $\beta$-lactamase genes, class 1 integrons, and chromosomally encoded and plasmid-mediated ampC genes. In addition, the epidemiological relationship was investigated by multilocus sequence typing. Among 61 carbapenem-resistant P. aeruginosa isolates, 25 isolates (41.0%) were MBL producers. Additionally, 30 isolates producing PDC (Pseudomonas-derived cephalosporinase)-2 were highly resistant to ceftazidime (MIC₅₀ = $256{\mu}g/ml$) and cefepime (MIC₅₀ = $256{\mu}g/ml$). Of all the PDC variants, 25 isolates harboring MBL genes showed high levels of cephalosporin and carbapenem resistance, whereas 36 isolates that did not harbor MBL genes revealed relatively low-level resistance (ceftazidime, p < 0.001; cefepime, p < 0.001; imipenem, p = 0.003; meropenem, p < 0.001). The coexistence of MBLs and AmpC $\beta$-lactamases suggests that these may be important contributing factors for cephalosporin and carbapenem resistance. Therefore, efficient detection and intervention to control drug resistance are necessary to prevent the emergence of P. aeruginosa possessing this combination of $\beta$-lactamases.

• 대한수의학회지
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• 제28권2호
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• pp.331-337
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• 1988

This paper dealt with the distribution of Salmonella (S) infection on 4 herds in Kyungju and Taegu during the period from May to October 1986. Isolated Salmonella were examined for serotypes, antimicrobial drug resistance and detection of R plasmid. The results obtained were summarised as followings: 1. Of total 4.622 samples from 4 herds, 67 Salmonella were isolated from 51 samples(1.1%), and their serovar strains were S typhimurium 6, S derby 5, S infantis 4, S bareilly 4, S dublin 3, S anatum 2, S montevideo 2 and untypable 41. 2. The isolation rate of Salmonella was higher in summer and autumn. 3. Of the 67 strains examined, 45 (67.2%) were resistant to one or more antibiotics, such as ampicillin (Am), cephalothin (Ce), chloramphenicol (Cm), rifampicin (Rf), sulfadimethoxine (Su), and tetracycline (Tc), and higher resistant to Sm (40.2%), Ce (31.3%), Am (23.9%). 4. Of the 45 resistant Salmonella strains, 44 (97.8%) harbored conjugative R plasmids and the transfer frequency of Sm (100%), Ce (95.2%), Tc (91.0%) and Su (80.0%) resistance was much higher than that of the other drug resistance. 5. The most common resistant patterns were Sm, Ce, AmCeCmSmSuTc, and AmCe. 6. In 4 herds, the incidience of drug resistance was 57.7%~100% and transfer frequency of conjugative R plasmid was 96.1%~100%.