• 호남수학학술지
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• 제20권1호
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• pp.45-55
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• 1998

• Journal of Microbiology and Biotechnology
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• 제8권3호
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• pp.214-221
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• 1998

A Brevibacterium ammoniagenes gene coding for glucose/mannose-specific enzyme II ($EII^{Glc}$) of the phosphoenolpyruvate-dependent phosphotransferase system (PTS) was cloned by complementing an Escherichia coli mutation affecting a ptsG gene, and the complete DNA nucleotide sequence was determined. The cloned gene was identified to be a ptsG, which enables the E. coli transportment to use glucose more efficiently than mannose as the sole carbon source in an M9 minimal medium. The ptsG gene of B. ammoniagenes consists of an open reading frame of 1,983 nucleotides putatively encoding a polypeptide of 661 amino acid residues and a TAA stop codon. The deduced amino acid sequence of the B. ammoniagenes $EII^{Glc}$ shows, at $46\%$, the highest degree of sequence similarity with the Corynebacterium glutamicum EII specific for both glucose and mannose. In addition, the $EII^{Glc}$ shares approximately $30\%$ sequence similarities with sucrose-specific and ${\beta}$-glucoside-specific EIIs of the several bacteria belonging to the glucose-PTS class. The 161-amino-acid C-terminal sequence of $EII^{Glc}$ is also similar to that of E. coli enzyme $IIA^{Glc}$, specific for glucose ($EIIA^{Glc}$). The B. ammoniagenes $EII^{Glc}$ consists of three domains; a hydrophobic region (EIIC) and two hydrophilic regions (EIIA, EIIB). The arrangement of structural domains, IIBCA, of the $EII^{Glc}$ is identical to those of EIIs specific for sucrose or ${\beta}$-glucoside. While the domain IIA was removed from the B. ammoniagenes $EII^{Glc}$ the remaining domains IIBC were found to restore the glucose and mannose-utilizing capacity of E. coli mutant lacking $EII^{Glc}$ activity with $EIIA^{Glc}$ of the E. coli mutant. $EII^{Glc}$ contains a histidine residue and a cysteine residue which are putative phosphorylation sites for the protein.

• 한국산림과학회지
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• 제104권2호
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• pp.213-220
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• 2015

본 연구는 부산 및 울산광역시의 토석류 유출 위험지역 총 15개소에서 토석류 유출에 영향을 미치는 산림 환경인자에 대한 인자별 발생분포비율을 분석하였다. 토심은 중(30~99 cm), 방위는 동사면과 북동사면 및 북서사면, 표고는 100~200 m, 산지평균경사는 $20^{\circ}$ 미만, 모암은 화성암, 과거 토석류 유출이 있었던 지역, 최대시우량은 50~100 mm, 1일 최대강우량은 300 m 이상, 수계밀도는 $3{\sim}4km/km^2$, 산사태 발생 가능성이 있는 경사 $20^{\circ}$ 이상의 분포비율은 20~40%와 40~60%, 산사태 위험도 2등급 이상 비율은 40~60%, 유목이 있는 상태, 사방공작물 시설이 없는 지역, 4영급, 임분이 밀한 지역, 혼효림 등의 인자가 토석류 발생에 기여하는 것으로 분석되었다.