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Studies on the Production of Protease by Aspergillus oryzae KC-15 and Characteristics of the Enzymes (Aspergillus oryzae KC-15에 의한 protease의 생산 및 그 효소의 특징에 관한 연구)

  • 이미자;정만재
    • Microbiology and Biotechnology Letters
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    • v.8 no.2
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    • pp.77-85
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    • 1980
  • This experiment was conducted to investigate the conditions for production and the characteristics of pretenses. Aspergillus oryzae KC-15, which is selected as a superior strain for the production of the protease, was used in this study. The results obtained were as follows: 1. The optimum culture time for the production of acid, neutral and alkaline protease on wheat bran medium were about 48, 48 and 72hr, respectively. The protease-produced by the strain were mainly alkaline and neutral one, but the production of acid protease was feeble extremely. 2. The addition of NaH$_2$PO$_4$, Na$_2$HPO$_4$, glucose, rice powder and Na-glutamate respectively to wheat bran media were effective for the production of alkaline and neutral protease, and the addition of (NH$_4$)$_2$HPO$_4$, glucose and rice powder respectively were effective for the production of acid protease. 3. Characteristics of professes(equation omitted) 4. As a heat resistance agent, NaH$_2$PO$_4$was the most effective one. The optimum amount of NaH$_2$PO$_4$was 10mg for alkaline and neutral protease, and 5mg for acid protease. 5. The heat resistance of the Protease by NaH$_2$PO$_4$was not recognized mostly above 6$0^{\circ}C$. 6. After the treatment of enzyme solution with 10mg of NaH$_2$PO$_4$for 30 minutes at 55$^{\circ}C$, the residual activities measured for alkaline, neutral and acid protease were 58, 57 and 55% respectively.

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Investigation of the Adsorption Phenomena of Surfactant on Hydrophilic Surface by Spectroscopic Method (분광학적 방법에 의한 계면활성제의 친수성 표면에 대한 흡착양상 연구)

  • Kim, Dong-Su
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.12
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    • pp.2215-2226
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    • 2000
  • Adsorption characteristics for homologous xanthates on quartz surface has been investigated by FT-IR spectroscopy to understand the adsorption mechanism in relation with verification of the influence of surfactants on the stability of hydrophilic suspended solids in aqueous system. Contact angle measurement for water droplets on quartz surface treated with xanthate was also conducted as a supplementary study for spectroscopic investigation to grant a reliability to the interpretation of experimental results. It has been observed that monolayer of ethyl xanthate was formed on quartz surface as the adsorption progressed and double layer of ethyl xanthate was formed eventually through the reverse oriented adsorption of xanthate molecules on the monolayer. Similar trend of adsorption to the case of ethyl xanthate was found for propyl and butyl xanthates and adsorption was observed to occur more rapidly as the number of carbon atom in alkyl group of xanthate homologs increased. Thermodynamic aspect of adsorption was discussed using Young's equation and the variation in adsorption rate was examined according to the concentration of ethyl xanthate. Based on the result, it has been concluded that the adsorption features of surfactant on substrate in aquatic system is governed by molecular structure and concentration of surfactant and its residual time in aqueous environment as dissolved state also influences greatly the stability of hydrophilic suspended particles.

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Introduction to Geophysical Exploration Data Denoising using Deep Learning (심층 학습을 이용한 물리탐사 자료 잡음 제거 기술 소개)

  • Caesary, Desy;Cho, AHyun;Yu, Huieun;Joung, Inseok;Song, Seo Young;Cho, Sung Oh;Kim, Bitnarae;Nam, Myung Jin
    • Geophysics and Geophysical Exploration
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    • v.23 no.3
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    • pp.117-130
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    • 2020
  • Noises can distort acquired geophysical data, leading to their misinterpretation. Potential noises sources include anthropogenic activity, natural phenomena, and instrument noises. Conventional denoising methods such as wavelet transform and filtering techniques, are based on subjective human investigation, which is computationally inefficient and time-consuming. Recently, many researchers attempted to implement neural networks to efficiently remove noise from geophysical data. This study aims to review and analyze different types of neural networks, such as artificial neural networks, convolutional neural networks, autoencoders, residual networks, and wavelet neural networks, which are implemented to remove different types of noises including seismic, transient electromagnetic, ground-penetrating radar, and magnetotelluric surveys. The review analyzes and summarizes the key challenges in the removal of noise from geophysical data using neural network, while proposes and explains solutions to the challenges. The analysis support that the advancement in neural networks can be powerful denoising tools for geophysical data.

Development of Low Temperature Thermal Desorption System and Remediation of Soil Contaminated with Petroleum Hydrocarbon (열순환식 저온열탈착 정화장치의 개발 및 유류오염 토양 현장 적용)

  • Kim, Guk-Jin;Lee, Sun-Hwa;Park, Kwang-Jin;Kim, Chi-Kyung;Lee, Cheol-Hyo;Kim, Do-Sun;Cho, Seok-Hee;Chang, Youn-Young
    • Journal of Soil and Groundwater Environment
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    • v.13 no.4
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    • pp.62-68
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    • 2008
  • The Low Temperature Thermal Desorption (LTTD) System equipped with a soil transfer unit, a rotary kiln, RTO, cyclones and a bag filter etc. was developed. The LTTD system was designed to be economically operated using LPG as a fuel and recirculating the discharged gas from the LTTD system through RTO. For the performance test of LTTD system the soil contaminated with light and heavy oils (2,690 mg TPH/kg soil) and with particle sizes below 50 mm was fed into the rotary kiln of LTTD system at 7$m^3$/hr with retention time of 15 minutes. Operation temperatures of LTTD system for the removal of soil TPH were $567^{\circ}C$ and $692^{\circ}C$. The residual TPH after treatment was 46 mg/kg and 32mg/kg respectively at each temperature condition, which shows high TPH removal efficiencies of the developed LTTD as 98.3% and 98.9%.

The single institutional outcome of postoperative radiotherapy and concurrent chemoradiotherapy in resected non-small cell lung cancer

  • Lee, Hyo Chun;Kim, Yeon Sil;Oh, Se Jin;Lee, Yun Hee;Lee, Dong Soo;Song, Jin Ho;Kang, Jin Hyung;Park, Jae Kil
    • Radiation Oncology Journal
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    • v.32 no.3
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    • pp.147-155
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    • 2014
  • Purpose: This study was conducted to observe the outcomes of postoperative radiotherapy (PORT) with or without concurrent chemotherapy in resected non-small cell lung cancer (NSCLC) in single institution. Materials and Methods: From 2002 to 2013, 78 patients diagnosed with NSCLC after curative resection were treated with radiotherapy alone (RT, n = 48) or concurrent chemoradiation (CCRT, n = 30). The indications of adjuvant radiation therapy were N2 node positive (n = 31), close or involved resection margin (n = 28), or gross residual disease due to incomplete resection (n = 19). The median radiation dose was 57.6 Gy (range, 29.9 to 66 Gy). Results: Median survival time was 33.7 months (range, 4.4 to 140.3 months). The 5-year overall survival (OS) rate was 49.5% (RT 46% vs. CCRT 55.2%; p = 0.731). The 3-year disease-free survival rate was 45.5% (RT 39.4% vs. CCRT 55.3%; p = 0.130). The 3-year local control rate was 68.1% (RT 64.4% vs. CCRT 77.7%; p = 0.165). The 3-year DMFS rate was 56.1% (RT 52.6% vs. CCRT 61.7%; p = 0.314). In multivariate analysis, age ${\geq}66$ years and pathologic stage III were significant poor prognostic factors for OS. Treatment failure occurred in 40 patients. Four patients had radiologically confirmed grade 3 radiation pneumonitis. Conclusion: In NSCLC, adjuvant RT or CCRT after curative surgery is a safe and feasible modality of treatment. OS gain was seen in patients less than 66 years. Postoperative CCRT showed a propensity of achieving better local control and improved disease-free survival compared to RT alone according to our data.

Wahsing Effect of Micor-Bubbles and Changes in Quality of Lettuce (Lacutuca sativa L.) during Storage (마이크로버블에 의한 상추의 세척효과 및 저장 중 품질변화)

  • Lee, Seon-Ah;Youn, Aye-Ree;Kwon, Ki-Hyun;Kim, Byeong-Sam;Kim, Sang-Hui;Cha, Hwan-Soo
    • Food Science and Preservation
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    • v.16 no.3
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    • pp.321-326
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    • 2009
  • We assessed quality changes in and washing effects (time and method) on lettuce (Lactuca sativa L.) treated with micro-bubbles. Samples were treated with micro-bubbling for 1, 3, or 5 min, and the 5-min treatment yielded the best results in terms of reduced total microorganism counts, sensory aspects, and degree of washing. Total microorganism counts were 4.30 log colony-forming units (CFU)/g in unwashed lettuce(CT), 4.10 log CFU/g in hand-washed lettuce (HW), 3.98 log CFU/g in conventional, bubble-washed lettuce (BW) and 3.25 log CFU/g in micro-bubble-washed lettuce (MW). In comparison, total counts of samples examined after 10 days of storage were 7.00 log CFU/g for CT, 6.19 log CFU/g for HW, 6.02 log CFU/g for BW, and 5.89 log CFU/g for MW. The lowest counts were seen after micro-bubble treatment. BW and MW samples showed significantly higher counts than did CT and HW samples. In general, BW and MW samples did not vary significantly in count numbers. MW showed a 2.3-fold lower residual pesticide level compared with CT, and also had the lowest level of impurities. HW and BW samples were not well washed.

Studies on the Influence of Various Factors on 2, 3, 5-Triphenyl Tetrazolium Chloride Reduction Test(T.T.C. Test) (2, 3, 5-Triphenyl Tetrazolium Chloride 환원시험(還元試驗)(T. T. C. Test)에 미치는 요인(要因)에 관한 연구(硏究))

  • Kim, Kyo-Joon;Kim, Sang Keun
    • Korean Journal of Agricultural Science
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    • v.5 no.2
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    • pp.80-86
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    • 1978
  • This study was carried out in order to know the effect of T. T. C. reaction on antibiotics, cleanser, disinfectants, pH, acidity and station of culture a bacterial Streptococcus thermophilus was used to 2.3.5-triphenyl tetrazolium chloride reduction test for the detection of residual antibiotics in milk were tested. The results obtained were summarized as follows. 1. The T. T. C. reaction was most sensitive in the group of penicillin as 0.02~0.05 IU and also it was less sensitive in Streptomycin and various broad antibiotics except Vibramycin, Synthomycin and Teramycin but it was least sensitive in Streptomycin group. 2. For ther T. T. C. reaction test on cleanser, the positive reaction was appeared above 0.7% of Hi-Ti and 0.1% of NaOH solution respectively. 3. For the T. T. C. test reaction on disinfectants, the positive reaction was occured on 200~500 ppm solution of sodium-hypochlorite and chloride-lime, 0.15~0.35% solution of phenol, cresol and Iode-K. 4. The optimum temperature was $35{\sim}37^{\circ}C$ and proper culturing time was 12 to 18 hours for propagation culturing. 5. The suspect positive reaction was tested above pH 5.0 and the positive reaction was appeared above 0.21% of acidity.

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Changes of Physicochemical Characteristics of Rice Milled by Newly Designed Abrasive Milling Machine (연삭식 도정기에 의한 도정 정도별 쌀의 이화학적 특성 변화)

  • 김용석;이나영;황철승;유미지;백경화;신동화
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.1
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    • pp.152-157
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    • 2004
  • Physicochemical characteristics of rice milled by newly designed abrasive milling machine were studied. Crude protein and crude lipid contents were 7.81∼8.50% and 1.07∼0.95%, respectively, and they showed a tendency to decrease with milling times. However, they showed higher values than that of polished rice. Major fatty acids of crude lipid were palmitic acid, oleic acid, and linoleic acid. Whiteness of tested samples was higher than brown rice but lower than polished rice. The remain rate of rice germ of four pass-through and polished one time sample (FPS) was 39.33%, and it was higher than that of polished rice (4.00%). One thousand grain weight of samples over than three pass-through was significantly different from brown rice (p<0.05). FPS had smooth surface similar to polished rice and it showed some residual rice germ by scanning electron microscopy. Sensory evaluation showed that the taste of three pass-through rice, four pass-through rice, and FPS was not significantly different from polished rice, whereas their color, odor, texture, and overall acceptability had lower score than polished rice but higher than brown rice. Therefore, we estimate that rice ground over three pass-through by newly designed abrasive milling machine might be a good source having nutritive value.

Physical and Sensory Characteristics of Bread Prepared by Substituting Sugar with Yacon Concentrate (설탕을 야콘 농축액으로 대체하여 제조한 빵의 물리적 및 관능적 특성)

  • Kim, Won-Mo;Kim, Mi-Kyung;Byun, Myung-Woo;Lee, Gyu-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.9
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    • pp.1288-1293
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    • 2012
  • Yacon (Smallanthus sonchifolius) contains high amounts of fructooligosaccharides and has been known to promote health of the intestinal tract and to have anti-oxidative and anti-cancer activities. Yacon concentrates were added to make five different pan breads, each with the addition 0% (YE 0), 25% (YE 25), 50% (YE 50), 75% (YE 75), and 100% (YE 100) of yacon concentrates instead of sugar. The higher the yacon concentration in the dough, the more time was needed for the dough to rise. Higher yacon concentration also affected the color of the bread by lowering the L-value and raising the a and b values. The hardness and chewiness of the bread significantly decreased with increasing yacon concentration, while cohesiveness increased. In the bread sensory evaluation, the intensity of crust color, crumb color, yacon flavor, sweetness, yacon taste, moistness, and residual mouth feel were increased with increasing yacon concentrates, while the uniformity of crumb pores slightly decreased. The consumer acceptance of taste, flavor, texture, and overall acceptance were increased with increasing yacon concentrations. As a conclusion, the substitution of yacon concentrates for sugar improved bread quality and increased consumer acceptance.

Atrial Fibrillation Waveform Extraction Algorithm for Holter Systems (홀터 심전계를 위한 심방세동 신호 추출 알고리즘)

  • Lee, Jeon;Song, Mi-Hye;Lee, Kyoung-Joung
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.49 no.3
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    • pp.38-46
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    • 2012
  • Atrial fibrillation is needed to be detected at paroxysmal stage and to be treated. But, paroxysmal atrial fibrillation ECG is hardly obtained with 12-lead electrocardiographs but Holter systems. Presently, the averaged beat subtraction(ABS) method is solely used to estimate atrial fibrillatory waves even with somewhat large residual error. As an alternative, in this study, we suggested an ESAF(event-synchronous adaptive filter) based algorithm, in which the AF ECG was treated as a primary input and event-synchronous impulse train(ESIT) as a reference. And, ESIT was generated so to be synchronized with the ventricular activity by detecting QRS complex. We tested proposed algorithm with simulated AF ECGs and real AF ECGs. As results, even with low computational cost, this ESAF based algorithm showed better performance than the ABS method and comparable performance to algorithm based on PCA(principal component analysis) or SVD(singular value decomposition). We also proposed an expanded version of ESAF for some AF ECGs with multi-morphologic ventricular activities and this also showed reasonable performance. Ultimately, with Holter systems including our proposed algorithm, atrial activity signal can be precisely estimated in real-time so that it will be possible to calculate atrial fibrillatory rate and to evaluate the effect of anti-arrhythmic drugs.