• Mycobiology
• /
• 제51권5호
• /
• pp.360-371
• /
• 2023

Hispidin is an important styrylpyrone produced by Sanghuangporus sanghuang. To analyze hispidin biosynthesis in S. sanghuang, the transcriptomes of hispidin-producing and non-producing S. sanghuang were determined by Illumina sequencing. Five PKSs were identified using genome annotation. Comparative analysis with the reference transcriptome showed that two PKSs (ShPKS3 and ShPKS4) had low expression levels in four types of media. The gene expression pattern of only ShPKS1 was consistent with the yield variation of hispidin. The combined analyses of gene expression with qPCR and hispidin detection by liquid chromatography-mass spectrometry coupled with ion-trap and time-of-flight technologies (LCMS-IT-TOF) showed that ShPKS1 was involved in hispidin biosynthesis in S. sanghuang. ShPKS1 is a partially reducing PKS gene with extra AMP and ACP domains before the KS domain. The domain architecture of ShPKS1 was AMP-ACP-KS-AT-DH-KR-ACP-ACP. Phylogenetic analysis shows that ShPKS1 and other PKS genes from Hymenochaetaceae form a unique monophyletic clade closely related to the clade containing Agaricales hispidin synthase. Taken together, our data indicate that ShPKS1 is a novel PKS of S. sanghuang involved in hispidin biosynthesis.

• 대한영상의학회지
• /
• 제81권1호
• /
• pp.231-236
• /
• 2020

십이지장 정맥류는 간경화증에 의한 문맥압 고혈압 환자에서 발생할 수 있으며, 치명적인 심한 상부 위장관 출혈을 일으킬 수 있으나 아직까지 명확한 치료 지침이 정립되어 있지는 않다. 일차적으로 내시경으로 치료를 시도하고 지혈 실패 시 목정맥경유간속문맥전신순환연결술, 풍선 혹은 혈관 마개를 이용한 역행성 경정맥 폐색술, 피부간경유 폐색술 등과 같은 중재적 치료를 고려할 수 있다. 저자들은 내시경적 치료에 실패한 십이지장 정맥류 출혈 환자에게 저하된 간 기능과 해부학적인 구조의 제한으로 인해 코일을 이용한 역행성 경정맥 폐색술을 시행하여 성공적으로 치료한 1예를 경험하였기에 이를 보고하고자 한다.

• Clinical Endoscopy
• /
• 제56권1호
• /
• pp.38-49
• /
• 2023

Background/Aims: Patients with Barrett's esophagus are at increased risk of developing esophageal adenocarcinoma. Endoscopic therapies aim to eradicate dysplastic and metaplastic tissues. Hybrid argon plasma coagulation (hybrid-APC) utilizes submucosal fluid injection to create a protective cushion prior to ablation that shields the submucosa from injury. We performed a pooled meta-analysis to evaluate the safety and efficacy of hybrid-APC. Methods: We conducted a systematic search of major electronic databases in April 2022. Studies that included patients with dysplastic and non-dysplastic Barrett's esophagus undergoing treatment with hybrid-APC were eligible for inclusion. Outcome measures included complete remission of intestinal metaplasia (CR-IM), stricture formation, serious adverse events, and number of sessions necessary to achieve CR-IM. Results: Overall pooled CR-IM rate for patients undergoing hybrid-APC was 90.8% (95% confidence interval [CI], 0.872-0.939; I²=0%). Pooled stricture rate was 2.0% (95% CI, 0.005-0.042; I²=0%). Overall serious adverse event rate was 2.7% (95% CI, 0.007-0.055; I²=0%). Conclusions: Results of the current meta-analysis suggest that hybrid-APC is associated with high rates of CR-IM and a favorable safety profile. Interpretation of these results is limited by the inclusion of retrospective cohort and case series data. Randomized controlled trials that standardize treatment and outcome evaluation protocols are necessary to understand how this treatment option is comparable to the current standards of care.

• Clinical and Experimental Reproductive Medicine
• /
• 제50권4호
• /
• pp.285-291
• /
• 2023

Objective: Infertility can result from a diminished ovarian reserve, but a potential remedy exists in the form of platelet-rich plasma (PRP) administration. This treatment involves both biological factors and tissue trauma mechanisms, which stimulate folliculogenesis, making it a promising and effective strategy. We assessed the impact of direct PRP injections into the ovaries on the fertility outcomes of women classified as poor responders. Methods: A quasi-experimental study was conducted from April 2021 to December 2022, focusing on patients classified as POSEIDON grade 3 or 4. PRP injections were administered into both ovaries. After 3 months, data were collected on anti-Mullerian hormone (AMH) level, follicle-stimulating hormone (FSH) level, and the numbers of oocytes, mature oocytes, and good-quality embryos following ovarian stimulation. We then compared the data from before and after PRP injection. Results: This study included 50 women, with a mean of 39 years (interquartile range [IQR], 35 to 43) and 4 years (IQR, 2 to 6) for age and infertility duration, respectively. FSH levels decreased after treatment, while AMH levels and the numbers of oocytes, metaphase II oocytes, and high-quality embryos increased. However, only the increase in high-quality embryos was significant. The pregnancy and spontaneous pregnancy rates were 20% and 14%, respectively. Notably, women with secondary infertility exhibited a significantly higher pregnancy rate than those with primary infertility. Conclusion: Ample evidence suggests that PRP can enhance ovarian function. However, further studies are needed to identify the appropriate candidates for this procedure, establish the optimal PRP preparation method, and standardize the procedure for its adjuvant use in assisted reproductive technology cycles.

• 한국동물생명공학회지
• /
• 제39권1호
• /
• pp.31-39
• /
• 2024

Background: The biological information of fish, which include reproduction, is the prerequisite and the basis for the assessment of fisheries. Methods: The aim of this work was to know the reproductive biology with the first sexual maturity (TL₅₀) and the spawning period for 58 mainly fish species in the waters around La Réunion Island (Western Indian Ocean). Twenty families belonging to the Actinopterygii were represented (acanthuridae, berycidae, bramidae, carangidae, cirrhitidae, gempylidae, holocentridae, kyphosidae, labridae, lethrinidae, lutjanidae, malacanthidae, monacanthidae, mullidae, polymixiidae, pomacentridae, scaridae, scorpaenidae, serranidae, sparidae; 56 species; n = 9,751) and two families belonging to the Elasmobranchii (squalidae, centrophoridae; 2 species; n = 781) were sampled. Between 2014 and 2022, 10,532 individuals were sampled covering the maximum months number to follow the reproduction periods of these species. Results: TL₅₀ for the males and the females, respectively, ranged from 103.9 cm (Acanthurus triostegus) to 1,119.3 cm (Thyrsitoides marleyi) and from 111.7 cm (A. triostegus) to 613.1 cm (Centrophorus moluccensis). The reproduction period could be very different between the species from the very tight peak to a large peak covered all months. Conclusions: Most species breed between October and March but it was not the trend for all species around La Réunion Island.

• Journal of Ecology and Environment
• /
• 제48권2호
• /
• pp.182-195
• /
• 2024

Background: Invasive alien plant species (IAP) significantly threaten Nepal's protected areas and local communities. Understanding their distribution, impact, management, and utilization is essential for developing effective management strategies and sustainable utilization practices. The systematic literature review of publications from 2010 to 2023. The search was conducted through the database Nepal Journal online database (NepJOL) and Google Scholar, yielding an initial pool of 4,304 publication. After applying inclusion and exclusion criteria; we meticulously reviewed 43 articles for data extraction. Results: Seventeen IAP are found in protected area, Nepal with the highest prevalence observed in Koshi Tappu Wildlife Reserve, followed by Chitwan and Sukhlaphanta National Park. The most problematic species in terrestrial ecosystems are Mikania micrantha, Lantana camara, and Chromolaena odorata. The grassland ecosystems of wildlife habitats, primarily in the Terai and Siwalik regions, are the most invaded. Various management approaches are employed to mitigate the spread and impact of IAP, including mechanical methods such as uprooting, burning, and cutting. However, these methods are costly, and context-specific interventions are needed. The study also explores the potential use of IAP for economic, ecological, or cultural purposes, such as medicinal properties, energy production potential, and economic viability. Local communities utilize these plants for animal bedding, mulching, green manure, briquette, and charcoal production. Conclusions: Applying silvicultural practices alongside mechanical management is recommended to maintain a healthy terrestrial ecosystem and utilize the removed biomass for valuable products, thereby reducing removal costs and increasing income sources, potentially benefitting both local communities and wildlife in protected areas.

• Clinical and Experimental Reproductive Medicine
• /
• 제51권2호
• /
• pp.158-162
• /
• 2024

Objective: The aim of this study was to compare the outcomes of in vitro fertilization (IVF) in patients with a poor ovarian response who used methyltestosterone, versus those using a placebo, in an infertility clinic setting. Methods: This clinical trial included 120 women who had undergone IVF with intracytoplasmic sperm injection due to poor ovarian reserve and infertility. The study took place at the Yas Infertility Center in Tehran, Iran, between January 1, 2018 and January 1, 2019. In the intervention group, 25 mg of methyltestosterone was administered daily for 2 months prior to the initiation of assisted reproductive treatment. The control group was given placebo tablets for the same duration before starting their cycle. Each group was randomly assigned 60 patients. All analyses were performed using SPSS ver. 23 (IBM Corp.). Results: The endometrial thickness in the intervention group was 7.57±1.22 mm, whereas in the control group, it was 7.11±1.02 (p=0.028). The gonadotropin number was significantly higher in the control group (64.7±13.48 vs. 57.9±9.25, p=0.001). However, there was no significant difference between the two groups in the antral follicular count. The chemical and clinical pregnancy rates in the intervention group were 18.33% and 15% respectively, compared to 8.33% and 6.67% in the control group. The rate of definitive pregnancy was marginally higher in the intervention group (13.3% vs. 3.3%, p=0.05). Conclusion: The findings of this study suggest that pretreatment with methyltestosterone significantly increases endometrium thickness and is associated with an increase in the definitive pregnancy rate.

• Structural Engineering and Mechanics
• /
• 제91권3호
• /
• pp.315-324
• /
• 2024

An expansive soil in 4970 special railway line in Dangyang City, China, has encountered a series of landslides due to the expansion characteristics of expansive soil over the past 50 years. Thereafter, a sheet-pile retaining structure was adopted to fortify the expansive soil slope after a comprehensive discussion. In order to evaluate the efficacy of engineering measure of sheet-pile retaining structure, the field test was carried out to investigate the lateral pressure and pile bending moment subjected to construction and service conditions, and the local daily rainfall was also recorded. It took more than 500 days to carry out the field investigation, and the general change laws of lateral pressure and pile bending moment versus local daily rainfall were obtained. The results show that the effect of rainfall on the moisture content of backfill behind the wall decreases with depth. The performance of sheet-pile retaining structure is sensitive to the intensity of rainfall. The arching effect is reduced significantly by employing a series of sheet behind piles. The lateral pressure behind the sheet exhibits a single-peak distribution. The turning point of the horizontal swelling pressure distribution is correlated with the self-weight pressure distribution of soil and the variation of soil moisture content. The measured pile bending moment is approximately 44% of the ultimate pile capacity, which indicates that the sheet-pile retaining structure is in a stable service condition with enough safety reserve.

• 보건교육건강증진학회지
• /
• 제24권5호
• /
• pp.119-135
• /
• 2007

스텝운동(bench-step aerobics: BSA)이 과체중 및 비만 우리나라 성인 여성을 위한 효과성, 안전성 및 실행 가능성을 갖춘 운동 프로그램이라는 것이 다수 연구자들에 의해 시사되어 왔다. 그러나 그 효과성은 일부 생리적 효과에 한하여 검증되었을 뿐 심리적 효과에 대해서는 거의 연구되지 않았다. 따라서 본 연구는 스텝운동 프로그램이 우리나라 과체중 및 비만(체질량지수 ${\geq}\;2\;kg/m^2$) 성인 여성에게 미치는 심리적 효과를 알아보기 위해 수행되었다. 이러한 목적을 달성하기 위해 보건소 방문자와 지역신문 및 보건소 홈페이지 홍보를 통해 모집된 여성 중 편의 추출한 운동군 15명과 대조군 13명의 분노(anger), 운동관련 자기 효능감(exercise self-efficacy), 운동관련 정서(exericse-related affect), 그리고 신체상(body image)을 운동 프로그램 전후에 측정하여 비교하였다. 자료 수집은 12주 스텝운동 프로그램(2006년 8월${\sim}$11월) 전과 후에 보건소에서 이루어졌다. 운동군에게 적용된 스텝운동 프로그램은 중강도[개인의 예비 심박 수(Heart Rate Reserve : HRR)의 $40/50{\sim}50/60%$]로 $45{\sim}60$분 동안 주 3회 이루어졌으며, 상기 운동 강도 및 운동 시간은 대상자의 적응도에 따라 12주에 걸쳐 점진적으로 증가되었다. 12주간의 스텝운동 후 운동군의 신체상이 유의하게 개선되었으며, 이는 대조군에서의 변화와도 유의한 차이를 보였다. 분노, 운동관련 자기 효능감, 그리고 운동관련 정서 또한 개선되는 경향이었으나 통계적으로 유의한 변화는 보이지 않았다. 이로써 우리나라 과체중 및 비만 성인 여성 대상 스텝운동의 심리적 효과가 부분적으로 지지되고 있으나, 현재까지 이루어진 관련 연구가 거의 없고 본 연구 또한 연구 설계상의 제한점을 갖고 있으므로 연구결과의 일반화를 위한 추후 연구가 요구된다. 특히 Floor effect 및 Ceiling effect 등에 의한 효과를 배제하기 위해 분노 수준이 상대적으로 더욱 높거나 운동관련 정서 수준이 상대적으로 낮은 과체중 및 비만여성을 대상으로 반복 연구를 수행할 필요가 있다.

• 한국도서관정보학회지
• /
• 제5권
• /
• pp.119-168
• /
• 1978

This is a survey of the reserved book system in the pilot universities in Korea. We have surveyed only 22 university libraries among 29 pilot schools as of 1977, because of the differences in the library users, library organization, library facilities, and library materials between universities and colleges. In 1972, the Korean Ministry of Education developed a reformation plan for their higher education based on the teaching method of curriculum-oriented faculty instead of that of the faculty-oriented curriculum. The former puts emphasis on the cultivation of a student's thinking, creativity, and judgement through self-teaching to do a given assignment. The reserved book system in a college or university library is one of the most important methods necessary to accomplish the above educational aim. The survey used a questionnaire with 50 question on 28 items concerning the various aspects of the reserved book system in 22 pilot universities. the survey result discovered many problems needing correction. The following list describes the measures needed to correct the problems found in the pilot universities. 1. The management of a centralized reserved book system is much more effective and economical than the decentralized reserved book system when a university is located on the same campus. 2. In the university library, an independent reserved book department requires to gain the desired educational aims as compared with the reserved book room controlled by any other department in the library. 3. The reserved book system should not be adopted by all the departments at once but enlarged gradually, for it needs the understanding and support of faculty members and the university itself. 4. As competence is essential to the effective operation of the reserved book room, the university library should not place an unqualified person in charge of the reserved book department. 5. The librarian in charge of the reserved book department is required to do more professional works such as analysis of users, collection and analysis of syllabuses, maintenance of faculty member cooperation, establishment of measures to acquire unavailable materials, and drawing up an effective management plan. However, he is spending most of his time in clerical works, that is, non-professional works. 6. Three to five titles of each reserved book are considered reasonable and required materials should be shelved in proportion to the number of students, that is, one copy per eight or ten students if the materials are allowed to lend for two hours at a time. For the supplementary materials, the library needs to place two or three copies per subject. 7. Professors must select reserved books with care so that they can be used year after year. 8. Few universities are asking professors the number of class students and the date when the reserved material will no longer be needed on reserve. 9. The library should gather all the lists of reserved books from every professor at least three to five months before the courses open, because it takes a long time to obtain foreign materials. 10. It is desirable that the reserved book department should collect the lists and prepare the materials with promptness and consistency. 11. Instead of block buying, it is desirable to purchase reserved books at the time the library gets the reserved book list from the professors. The library should also inform faculty members whether it obtained each reserved book or not before the course open. 12. The library should make a copy of materials if a professor requires to reserve an out-of-print book or partial contents of a book, journal, and thesis. 13. An independent budger for reserved books from the budget for general materials is desired. 14. The shelf arrangement of reserved books by courses or professors under the same department is much more preferable than a classified arrangement. 15. While most of the universities adopted the open shelves system for all the reserved books, it is more effective and economical to take a compromise system, that is, closed shelves for requires materials and open shelves for supplementary materials. 18. Circulation of reserved books needs a different system between required materials and supplementary materials: two or three hours and/or overnight loan for the former and two and/or three days loan for the latter. 17. A reserved book room should be open a long time after class so that students can have sufficient time to use the room. 18. The library must take daily and monthly statistic as well as statistics on every aspect of the reserved book system in order that the library ma decide on policy and management of the reserved book room in collaboration with the university. Furthermore, regular reports on the use of the reserved book room should be made to the president and the executive council by the library to acquire their understanding and cooperation for the reserved book system. 19. Cooperation of faculty members is indispensable to the effective management of the reserved book department and it is desirable to make a committee which will fix various decisions about the system. Whenever the director of the library make his decision, he must consult with his staff in order to involve them earnestly in the operation of the system.