• Molecules and Cells
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• 제44권7호
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• pp.458-467
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• 2021

GPR43 (also known as FFAR2 or FFA2) is a G-protein-coupled receptor primarily expressed in immune cells, enteroendocrine cells and adipocytes that recognizes short-chain fatty acids, such as acetate, propionate, and butyrate, likely to be implicated in innate immunity and host energy homeostasis. Activated GPR43 suppresses the cAMP level and induces Ca²⁺ flux via coupling to Gα_i and Gα_q families, respectively. Additionally, GPR43 is reported to facilitate phosphorylation of ERK through G-protein-dependent pathways and interacts with β-arrestin 2 to inhibit NF-κB signaling. However, other G-protein-dependent and independent signaling pathways involving GPR43 remain to be established. Here, we have demonstrated that GPR43 augments Rho GTPase signaling. Acetate and a synthetic agonist effectively activated RhoA and stabilized YAP/TAZ transcriptional coactivators through interactions of GPR43 with Gα_q/11 and Gα_12/13. Acetate-induced nuclear accumulation of YAP was blocked by a GPR43-specific inverse agonist. The target genes induced by YAP/TAZ were further regulated by GPR43. Moreover, in THP-1-derived M1-like macrophage cells, the Rho-YAP/TAZ pathway was activated by acetate and a synthetic agonist. Our collective findings suggest that GPR43 acts as a mediator of the Rho-YAP/TAZ pathway.

• 한국비블리아학회지
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• 제33권1호
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• pp.345-369
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• 2022

본 연구의 목적은 인문학 분야 연구데이터와 연구논문을 연계할 수 있는 '인문학 분야 연구데이터를 위한 연구논문 의미구조 기반 메타데이터'를 개발하는 것이다. 인문학 분야 연구자의 관심 정보를 파악하기 위해 키워드 유형화를 진행하고 이를 일반적인 연구논문의 의미구조를 기준으로 분석함으로써 인문학 분야의 특성을 반영하기 위해 추가될 요소를 확인하였다. 이를 기반으로 인문학 분야 연구논문의 의미구조 기반 메타데이터를 구성하였으며 해당 메타데이터가 인문학 분야 연구데이터의 메타데이터로서 적합한지를 평가하기 위하여 인문학 분야 연구자를 대상으로 설문을 진행하였다. 인문학 분야 연구논문의 의미구조를 기반으로 도출된 17개의 메타데이터 요소 중 인문학 분야 연구데이터 메타데이터로 활용하기에 적합하다고 판단된 요소는 7개이며, 그 외 10개 요소는 사용 가능한 요소로 확인되었다. 평가 결과를 바탕으로 인문학 분야 연구데이터를 위한 연구논문 의미구조기반 메타데이터를 구성하였다.

• 한국IT서비스학회지
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• 제17권2호
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• pp.143-163
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• 2018

The business models has a great impact on the successful management of enterprises. Business environment has been shifting from industrial economy to knowledge-based economy. Enterprises go through numerous trials for successful management in the changing environment. Along with trial tests, research areas have been growing simultaneously. Although many researches have been conducted with regard to business models, it is very insufficient to systematically analyze the knowledge flow of research. Accordingly, successive researchers who want to study the business model may find it difficult to establish the orientation of future application research based on understanding the process of changing the knowledge structure that have accumulated so far. This study is intended to determine the current state of the business model research and to understand the process of knowledge structure changes in keywords that appear in 2,667 business model articles in the SCOPUS database. Identifying the knowledge structure has been completed through social network analysis, a methodology based on the 'relationship', and the changes in the knowledge structure were identified by classifying them into four different periods. The analysis showed that, first, the number of business model co-author increases over time with the need for academic diversity. Second, the 'innovation' keyword has the biggest center in the network, and over time, the lower-rank keyword which was in the former period has emerged as the top-rank keyword. Third, the cohesiveness group decreased from 12 before 2000 to 5 in 2015 and also the modularity decreased as well. Finally, examining characteristics of study area through a cognitive map showed that the relationships between domains increased gradually over time. The study has provided a systematic basis for understanding the current state of the business model research and the process of changing knowledge structure. In addition, considering that no research has ever systematically analyzed the knowledge structure accumulated by individual researches, it is considered as a significant study.

• 천문학회보
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• 제32권2호
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• pp.75.1-75.1
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• 2007

• Nuclear Engineering and Technology
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• 제41권10호
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• pp.1323-1332
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• 2009

The IHX (Intermediate Heat eXchanger) for a pool-type SFR (Sodium-cooled Fast Reactor) system transfers heat from the primary high temperature sodium to the intermediate cold temperature sodium. The upper structure of the IHX is a coaxial structure designed to form a flow path for both the secondary high temperature and low temperature sodium. The coaxial structure of the IHX consists of a central downcomer and riser for the incoming and outgoing intermediate sodium, respectively. The IHX of a pool-type SFR is supported at the upper surface of the reactor head with an IHX support structure that connects the IHX riser cylinder to the reactor head. The reactor head is generally maintained at the low temperature regime, but the riser cylinder is exposed in the elevated temperature region. The resultant complicated temperature distribution of the co-axial structure including the IHX support structure may induce a severe thermal stress distribution. In this study, the structural feasibility of the current upper support structure concept is investigated through a preliminary stress analysis and an alternative design concept to accommodate the IHTS (Intermediate Heat Transport System) piping expansion loads and severe thermal stress is proposed. Through the structural analysis it is found that the alternative design concept is effective in reducing the thermal stress and acquiring structural integrity.

• BMB Reports
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• 제49권10호
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• pp.560-565
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• 2016

Granzyme A (GzmA) was first identified as a cytotoxic T lymphocyte protease protein with limited tissue expression. A number of cellular proteins are known to be cleaved by GzmA, and its function is to induce apoptosis. Histones H1, H2B, and H3 were identified as GzmA substrates during apoptotic cell death. Here, we demonstrated that histone H4 was cleaved by GzmA during staurosporine-induced cell death; however, in the presence of caspase inhibitors, staurosporine-treated Raji cells underwent necroptosis instead of apoptosis. Furthermore, histone H4 cleavage was blocked by the GzmA inhibitor nafamostat mesylate and by GzmA knockdown using siRNA. These results suggest that histone H4 is a novel substrate for GzmA in staurosporine-induced cells.

• Biodesign
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• 제6권4호
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• pp.100-102
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• 2018

Autophagy is a degradation pathway that targets many cellular components and plays a particularly important role in protein degradation and recycling. This process is very complex and several proteins participate in this process. One of them, P62/SQSTM1, is related to the N-end rule and induces protein degradation through autophagy. The P62/SQSTM1 makes a huge oligomer, and this oligomerization is known to play an important role in its mechanism. This oligomerization takes two steps. First, the PB1 domain of P62/SQSTM1 makes the base oligomer, and then, when the ligand binds to the ZZ domain of P62/SQSTM1, it induces a higher oligomer by the disulfide bond of the two cysteines. To understand the oligomerization mechanism of P62/SQSTM1, we need to know the dimerization of the PB1 domain. In this study, crystals of PB1 dimer were made and the crystals were diffracted by X-ray to collect usable data up to 3.2A. We are analyzing the structure using the molecular replacement (MR) method.

• 한국해양공학회지
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• 제34권4호
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• pp.263-271
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• 2020

In this study, we performed a three-dimensional (3D) topology optimization of a fixed offshore structure to enhance its structural stiffness. The proposed topology optimization is based on the solid isotropic material with penalization (SIMP) method, where a volume constraint is applied to utilize an equivalent amount of material as that used for the rule-based scantling design. To investigate the effects of the main legs of the fixed offshore structure on its structural stiffness, the leg region is selectively considered in the design domain of the topology optimization problem. The obtained optimal designs and the rule-based scantling design of the structure are manufactured by 3D metal printing technology to experimentally validate the topology optimization. The behaviors under compressive loading of the obtained optimal designs are compared with those of the rule-based scantling design using a universal testing machine (UTM). Based on the structural experiments, we concluded that by employing the topology optimization method, the structural stiffness of the structure was enhanced compared to that of the rule-based scantling design for an equal amount of the fabrication material. Furthermore, by effectively combining the topology optimization and rule-based scantling methods, we succeeded in enhancing the structural stiffness and improving the breaking load of the fixed offshore structure.

• 한국해양공학회지
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• 제35권3호
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• pp.191-202
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• 2021

Studies on very large offshore structures are increasing owing to the development of deep sea, large-scale energy generation using ocean resources, and so on. The enlargement of offshore structures makes the hydroelastic effect and low natural frequency related responses important. Numerical analyses and model tests for hydroelastic and higher-order springing responses of fixed cylindrical structures are conducted in this study. The panel methods with and without the hydroelastic effect with shell elements, and the Morison analysis method with beam elements are applied. To observe the hydroelastic effect for structural strength, two structures are considered: bottom-fixed cylindrical structures with high and low bending stiffnesses, respectively. The surge motions at the top of the structure and bending stresses on the structure are observed under regular and irregular wave conditions. The regular wave conditions are generated considering the ratios of the cylindrical outer diameter to the wave lengths, and keeping the wave steepness constant. The model tests are performed in the three-dimensional ocean engineering basin in the KRISO (Korea Research Institute of Ships and Ocean Engineering). From the numerical and experimental results, in which the hydroelastic responses are only observed in the case of the structure with a low bending stiffness, it is confirmed that the hydroelastic responses are highly dependent on the structural stiffness. Additionally, the higher-order phenomenon on the specified wave condition is analyzed by observing the higher-order springing responses when the incident wave frequency or its multiples with the high wave height coincides with the natural frequency of the structure.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 1996년도 정기총회 및 학술발표회
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• pp.10-10
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• 1996

High-resolution solution conformation of a novel conotoxin, [Pro 7,13] $\alpha$A-conotoxin $P_{IVA}$, GCCGSYPNAACHPCSCKDROSYCGQ-N $H_2$, has been determined by two-dimensional $^1$H nmr methods in combination with distance geometry calculation to rmsd values of 0.90 $\AA$ and 1.16 $\AA$ for the backbone and heavy atoms, respectively. Total of 324 NOE-derived interproton distance restraints including 33 long-range NOE restraints a well as 11 $\Phi$ and 7 $\chi$$^1$ torsion angle restraints were used for computation of structures. (omitted)d)