• Journal of Microbiology and Biotechnology
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• v.34 no.5
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• pp.1154-1163
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• 2024

Glucosylation is a well-known approach to improve the solubility, pharmacological, and biological properties of flavonoids, making flavonoid glucosides a target for large-scale biosynthesis. However, the low yield of products coupled with the requirement of expensive UDP-sugars limits the application of enzymatic systems for large-scale. C. glutamicum is a Gram-positive and generally regarded as safe (GRAS) bacteria frequently employed for the large-scale production of amino acids and biofuels. Due to the versatility of its cell factory system and its non-endotoxin producing properties, it has become an attractive system for the industrial-scale biosynthesis of alternate products. Here, we explored the cell factory of C. glutamicum for efficient glucosylation of flavonoids using apigenin as a model flavonoid, with the heterologous expression of a promiscuous glycosyltransferase, YdhE from Bacillus licheniformis and the endogenous overexpression of C. glutamicum genes galU1 encoding UDP-glucose pyrophosphorylase and pgm encoding phosphoglucomutase involved in the synthesis of UDP-glucose to create a C. glutamicum cell factory system capable of efficiently glucosylation apigenin with a high yield of glucosides production. Consequently, the production of various apigenin glucosides was controlled under different temperatures yielding almost 4.2 mM of APG1(apigenin-4'-O-β-glucoside) at 25℃, and 0.6 mM of APG2 (apigenin-7-O-β-glucoside), 1.7 mM of APG3 (apigenin-4',7-O-β-diglucoside) and 2.1 mM of APG4 (apigenin- 4',5-O-β-diglucoside) after 40 h of incubation with the supplementation of 5 mM of apigenin and 37℃. The cost-effective developed system could be used to modify a wide range of plant secondary metabolites with increased pharmacokinetic activities on a large scale without the use of expensive UDP-sugars.

• Journal of Bio-Environment Control
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• v.32 no.1
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• pp.48-56
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• 2023

High-pressure sodium (HPS) lamps have been widely used as a useful supplemental light source to emit sufficient photosynthetically active radiation and provide a radiant heat, which contribute the heat requirement in greenhouses. The objective of this study to analyze the thermal characteristics of HPS lamp and thermal behavior in supplemented greenhouse, and evaluate the performance of a horizontal leaf temperature of sweet pepper plants using computational fluid dynamics (CFD) simulation. We simulated horizontal leaf temperature on upper canopy according to three growth stage scenarios, which represented 1.0, 1.6, and 2.2 plant height, respectively. We also measured vertical leaf and air temperature accompanied by heat generation of HPS lamps. There was large leaf to air temperature differential due to non-uniformity in temperature. In our numerical calculation, thermal energy of HPS lamps contributed of 50.1% the total heat requirement on Dec. 2022. The CFD model was validated by comparing measured and simulated data at the same operating condition. Mean absolute error and root mean square error were below 0.5, which means the CFD simulation values were highly accurate. Our result about vertical leaf and air temperature can be used in decision making for efficient thermal energy management and crop growth.

• Journal of radiological science and technology
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• v.26 no.2
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• pp.37-42
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• 2003

Purpose : Computed tomographic equipment is essential for diagnosis by means of radiation. With passage of time and development of science computed tomographic was developed time and again and in future examination by means of this equipment is expected to increase. In this connection these authors measured rate of scatter ray generation at front of lead glass for patients within control room of computed tomographic equipment room and outside of entrance door for exit and entrance of patients and attempted to ind out method for minimizing exposure to scatter ray. Material and Method : From November 2001 twenty five units of computed tomographic equipments which were already installed and operation by 13 general hospitals and university hospitals in Seoul were subjected to this study. As condition of photographing those recommended by manufacturer for measuring exposure to sauter ray was use. At the time objects used DALI CT Radiation Dose Test Phantom fot Head (${\oint}16\;cm$ Plexglas) and Phantom for Stomache(${\oint}32\;cm$ Plexglas) were used. For measurement of scatter ray Reader (Radiation Monitor Controller Model 2026) and G-M Survey were used to Survey Meter of Radical Corporation, model $20{\times}5-1800$, Electrometer/Ion Chamber, S/N 21740. Spots for measurement of scatter ray included front of lead glass for patients within control room of computed tomographic equipment room which is place where most of work by gradiographic personnel are carried out and is outside of entrance door for exit and entrance of patients and their guardians and at spot 100 cm off from isocenter at the time of scanning the object. The results : Work environment within computed tomography room which was installed and under operation by each hospital showed considerable difference depending on circumstances of pertinent hospitals and status of scatter ray was as follows. 1) From isocenter of computed tomographic equipment to lead glass for patients within control room average distance was 377 cm. At that time scatter ray showed diverse distribution from spot where no presence was detected to spot where about 100 mR/week was detected. But it met requirement of weekly tolerance $2.58{\times}10^{-5}\;C/kg$(100 mR/week). 2) From isocenter of computed tomographic equipment to outside of entrance door where patients and their guardians exit and enter was 439 cm in average, At that time scatter ray showed diverse distribution from spot where almost no presence was detected to spot with different level but in most of cases it satisfied requirement of weekly tolerance of $2.58{\times}10^{-6}\;C/kg$(100 mR/week). 3) At the time of scanning object amount of scatter ray at spot with 100 cm distance from isocenter showed considerable difference depending on equipments. Conclusion : Use of computed tomographic equipment as one for generation of radiation for diagnosis is increasing daily. Compared to other general X-ray photographing field of diagnosis is very high but there is a high possibility of exposure to radiation and scatter ray. To be free from scatter ray at computed tomographic equipment room even by slight degree it is essential to secure sufficient space and more effort should be exerted for development of variety of skills to enable maximum photographic image at minimum cost.

• Journal of Soil and Groundwater Environment
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• v.13 no.1
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• pp.32-42
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• 2008

A surfactant recovery and reuse process by selective adsorption with activated carbon was proposed to reduce surfactant cost in a soil washing process. Mathematical model simulation was performed for the whole process, which consists of soil washing, soil recovery, and soil re-washing. The optimal range of surfactant dosage was $6{\sim}10$-fold critical micelle concentration in soil. The efficiency of surfactant reuse process was decreased with increasing the dosage of activated carbon. Effectiveness factor for activated carbon significantly altered the efficiency of the reuse process unlike effectiveness factor for soil. Total requirement of surfactant was reduced to 20-30% with the reuse process compared to the conventional soil washing process. The contamination of wastewater after soil washing was reduced with the reuse process. This mathematical model can be used to estimate performance of the whole process of soil washing including surfactant recovery and to obtain optimal ranges of operating conditions without extra labor-intensive experimental works.

• Journal of Biosystems Engineering
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• v.12 no.2
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• pp.16-27
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• 1987

Greenhouse farming was introduced to the Korean farmers in the middle of 1950's and its area has been increased annually. The plastic greenhouse, which is covered with polyethylene or polyvinyl chloride film, has been rapidly spread in greenhouse farming since 1970. The greenhouse farming greatly contributed to the increase of farm household income and the improvement of crop productivity per unit area. Since the greenhouse farming is generally practiced during winter, from November to March, the thermal environment in the plastic greenhouse should be controlled in order to maintain favorable condition for plant growing. Main factors that influence the thermal environment in the plastic greenhouse are solar radiation, convective and radiative heat transfer among the thermal component of the greenhouse, and the use of heat source. The objective of this study was to develop a simulation model for thermal environment of the plastic greenhouse in order to determine the characteristics of heat flow and effects of various ambient environmental conditions upon thermal environments within the plastic greenhouse. The results obtained are summarized as follows: 1. Simulation model for thermal environment of the plastic greenhouse was developed, resulting in a good agreement between the experimental and predicted data. 2. Solar radiation being absorbed in the plant and soil during the daytime was 75 percent of the total solar radiation and the remainder was absorbed in the plastic cover. 3. About 83 percent of the total heat loss was due to convective and radiative heat transfer through the plastic cover. Air ventilation heat loss was 5 to 6 percent of total heat loss during the daytime and 16 to 17 percent during the night. 4. The effectiveness of thermal curtain for the plastic greenhouse at night was significantly increased by the increase of the inside air temperature of the greenhouse due to the supplementary heat. 5. When the temperature difference between the inside and outside of the greenhouse was small, the variation of ambient wind velocity did not greatly affect on the inside air temperature. 6. The more solar radiation in the plastic greenhouse was, the higher the inside air temperature. Because of low heat storage capacity of the plant and soil inside the greenhouse and a relatively high convective heat loss through the plastic cover, the increase of solar radiation during the daytime could not reduce the supplymentary heat requirement for the greenhouse during the night.

• Culinary science and hospitality research
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• v.22 no.3
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• pp.240-253
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• 2016

The purpose of this study is to predict factors influencing participant demand for the temple stays and to help find alternatives for temple stay marketing strategies. Specifically, the study sought to examine input variables on the visit frequency of temple visitors who partook in temple food. Research subjects were temple stay participants with experience with temple food. Through convenience sampling method, 300 self-administered questionnaires were distributed to participants at 4 temple stays in Seoul. Of the 278 questionnaires collected, 232 (83%) were used for research analysis. Given that the requirement that proper model for analysing the collected data be applied, the Truncated Negative Binomial(TNB) Poisson model, which is useful for analysing count data that are truncated at '0' and overcrowded with a certain value, was selected fort his study. Study results found that, for temple stay food revitalization, the most crucial item for temple food proponents to recognize is natural food ingredients. The degree of affection was higher among respondents over 40 years of groups and with incomes over 40 million won or more than others. In addition, unmarried and male were higher than married and female, and the Christian population in the temple food demand higher impact than Shamanism community. This match should be a priority to establish an in-depth public relations policy of targeted marketing of consumers according to various demographic characteristics. Active and aggressive efforts to expand food inspection are required to promote the healthy image of the temple food to the fragmentation of consumer marketing hierarchy.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.41-41
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• 2015

BSA-seq technologies, combined Bulked Segregant Analysis (BSA) and Next-Generation Sequencing (NGS), are making it faster and more efficient to establish the association of agronomic traits with molecular markers or candidate genes, which is the requirement for marker-assisted selection in molecular breeding. Clubroot disease, caused by Plasmodiophora brassicae, is a serious threat to Brassica crops. Even we have breed new clubroot resistant varieties of Chinese cabbage (B. rapa ssp. pekinesis), the underlying genetic mechanism is unclear. In this study, an $F_2$ population of 340 plants were inoculated with P. brassicae from Xinye (Pathotype 2 on the differentials of Williams). Resistance phenotype segregation ratio for the populations fit a 3:1 (R:S) segregation model, consistent with a single dominant gene model. Super-BSA, using re-sequencing the parents, extremely R and S DNA pools with each 50 plants, revealed 3 potential candidate regions on the chromosome A03, with the most significant region falling between 24.30 Mb and 24.75 Mb. A linkage map with 31 markers in this region was constructed with several closely linked markers identified. A Major QTL for clubroot resistance, CRq, which was identified with the peak LOD score at 169.3, explaining 89.9% of the phenotypic variation. And we developed a new co-segregated InDel marker BrQ-2. Joint BSA-seq and traditional QTL analysis delimited CRq to an 250 kb genomic region, where four TIR-NBS-LRR genes (Bra019409, Bra019410, Bra019412 and Bra019413) clustered. The CR gene CRq and closely linked markers will be highly useful for breeding new resistant Chinese cabbage cultivars.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2010.11a
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• pp.14-17
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• 2010

Following the proliferation of three-dimensional video contents and displays, many terrestrial broadcasting companies prepare for starting stereoscopic 3DTV service. In terrestrial stereoscopic broadcast, it is a difficult task to code and transmit two video sequences while sustaining as high quality as 2DTV broadcast attains due to the limited bandwidth defined by the existing digital TV standards such as ATSC. Thus, a terrestrial 3DTV broadcasting system with heterogeneous video coding systems is considered for terrestrial 3DTV broadcast where the left image and right images are based on MPEG-2 and H.264/AVC, respectively, in order to achieve both high quality broadcasting service and compatibility for the existing 2DTV viewers. Without significant change in the current terrestrial broadcasting systems, we propose a joint rate control scheme for stereoscopic 3DTV service. The proposed joint rate control scheme applies to the MPEG-2 encoder a quadratic rate-quantization model which is adopted in the H.264/AVC. Then the controller is designed for the sum of two bit streams to meet the bandwidth requirement of broadcasting standards while the sum of image distortions is minimized by adjusting quantization parameter computed from the proposed optimization scheme. Besides, we also consider a condition on quality difference between the left and right images in the optimization. Experimental results demonstrate that the proposed bit rate control scheme outperforms the rate control method where each video coding standard uses its own bit rate control algorithm in terms of minimizing the mean image distortion as well as the mean value and the variation of absolute image quality differences.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.48 no.6
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• pp.455-466
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• 2020

In this study, we built a thermal model for SNIPE 6U nano-satellite which has scientific mission for measuring science data in near Earth space environment and described thermal design based on the thermal model. And the validity of the thermal design was verified through the on-orbit thermal analysis. The thermal design was carried out mainly on the passive thermal control techniques such as surface finishes, insulators, and thermal conductors in consideration of the characteristics of the nano-satellite. However, the components with narrow operating temperature range and directly exposed to the orbital thermal environments, such as a battery and thrusters, are accomodated with heaters to satisfy the temperature requirements. On-orbit thermal analysis conditions are based on the basic orbital conditions of the satellite, and thermal analysis was performed for Normal mode, Launch & Early Orbit Phase (LEOP), Safehold mode, and Maneuver mode which are classified by the power consumption and the attitude of the satellite according to the mission scenario. The analysis results for each mode confirmed that every component satisfies the temperature requirement. In addition, the heater capacity and duty cycle of the battery and thruster were calculated through the analysis results of the Safehold mode.

• Journal of the Earthquake Engineering Society of Korea
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• v.14 no.3
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• pp.31-38
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• 2010

The purpose of this study is to evaluate the seismic performance of gravity-designed post tensioned (PT) flat plate frames with and without slab bottom reinforcement passing through the column. In low and moderate seismic regions, buildings are often designed considering only gravity loads. This study focuses on the seismic performance of gravity load designed PT flat plate frames. For this purpose, 3-, 6- and 9-story PT flat plate frames are designed considering only gravity loads. For reinforced concrete flat plate frames, continuous slab bottom reinforcement (integrity reinforcement) passing through the column should be placed to prevent progressive collapse; however, for the PT flat plate frames, the slab bottom reinforcement is often omitted since the requirement for the slab bottom reinforcement for PT flat plates is not clearly specified in ACI 318-08. This study evaluates the seismic performance of the model frames, which was evaluated by conducting nonlinear time history analyses. For conducting nonlinear time history analyses, six sets of ground motions are used as input ground motions, which represent two different hazard levels (return periods of 475 and 2475 years) and three different locations (Boston, Seattle, and L.A.). This study shows that gravity designed PT flat plate frames have some seismic resistance. In addition, the seismic performance of PT flat plate frames is significantly improved by the placement of slab bottom reinforcement passing through the column.