• 한국가축번식학회지
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• 제13권2호
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• pp.93-97
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• 1989

These experiments were carried out to develop the technique of nuclear transplantation necessary for elevating utilization efficiency of high quality embryos and the production of clone animals. Embryos of pronucleus stages were obtained from ICR mice. Removal of pronuclei and their transfer to recipient embryos were carried out by micromanipulation and virus mediation. The results obtained in these experiments were summarized as follows ; 1. Total 337 pairs of pronuclear stage embryos were subjected to nuclear transplantatin and 247 pairs(73.3%) of them were successfully transplanted and the number of fused embryos between transplanted nucleus and cytoplasm was 188 pairs(55.8%). 2. Of the 188 fused embryos cultured in vitro, 174(92.4%), 131(69.7%) and 117(62.2%) embryos were developed to 2-cell, morula and blastocyst stages, respectively. 3. When total 104 nuclear transplanted embryos were transferred to uteri of recipient mice on day 2-3 of pseudopregnancy, 4 of 12 recipient mice were pregnant and the number of embryos developed to young was 28(26.9%).

• Asian-Australasian Journal of Animal Sciences
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• 제10권4호
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• pp.408-415
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• 1997

Data from 5,311 ewes and 13,076 lambing from 1977 through 1994 were used to analyse both annual and cumulative outputs in terms on total number of lambs born, total lamb weight weaned and total wool produced per ewe for ewe longevity 1 to 8 depending on their productive life in the flock. Ewes at Khushab produced 0.08 more lambs per parturition than ewes at Khizerabad; however, 0.39 less lambs were weaned at Khushab than at Khizerabad. Similarly, cumulative number of lambs born was more at Khushab flock than Khizerabad flock (p < .01). However, total weight of lambs weaned was greater at Khizerabad than Khushab flock (p < .01) for each ewe longevity. Most ewes (35%) were sold/replaced just after their first parturition (i. e. ewe longevity 1). The overall mean for annual sale/replacement was 32 and 23% at Khushab and Khizerabad, respectively. Distribution of growth and reproductive traits from 1977-94 did not show upward or downward trend inspite of heavy sale/replacement except yearly variation. Lack of any genetic progress over the year suggested that random breeding was employed without any scientific selection programme. Annual means for lambs born, lambs weaned and weight of lambs weaned per ewe present in the flock were the highest for ewe longevity 2 compared with other ewe longevity groups. Relative efficiency in terms of net income was highest for ewe longevity 5 followed by ewe longevity 4 and 6 in both flocks.

• 한국공간구조학회논문집
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• 제1권1호
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• pp.125-134
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• 2001

The objective of this study is the development of size discrete optimum design algorithm which is based on the GAs(genetic algorithms). The algorithm can perform size discrete optimum designs of space trusses. The developed algorithm was implemented in a computer program. For the optimum design, the objective function is the weight of space trusses and the constraints are limite state design codes(1998) and displacements. The basic search method for the optimum design is the GAs. The algorithm is known to be very efficient for the discrete optimization. This study solves the problem by introducing the GAs. The GAs consists of genetic process and evolutionary process. The genetic process selects the next design points based on the survivability of the current design points. The evolutionary process evaluates the survivability of the design points selected from the genetic process. In the genetic process of the simple GAs, there are three basic operators: reproduction, cross-over, and mutation operators. The efficiency and validity of the developed discrete optimum design algorithm was verified by applying GAs to optimum design examples.

• 한국가축번식학회지
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• 제20권3호
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• pp.225-232
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• 1996

The purpose of this study was to evaluate effects of hormonal treatments on corpus lutea, follicles and development stage of embryos for enhancing the production efficiency of in vivo porcine embryos suitable to introduce fo foreign genes. Hundred and twenty gilts were allocated to 6 experimental group in different combinations of hormones PG 600, PMSG, hCG and altrenogest. When gilts were treated with chorionic gonadotrophin 200 IU and serum gonadotrophin 200 IU(PG 600), altrenogest, serum gonadotrophin (PMSG) 1,000 IU, and chorionic gonadotrophin(hCG) 750 IU (PAPh), the numbers of corpus luteum (30.4) were significantly higher than those of other treatment groups (P<0.05). The number of corpus luteum from ovary in either right (9.1) or left (10.1) side was not significantly changed with hormone treatments. Number of follicles in control was 20.7, which was higher than those of hormonal treatment groups. The average numbers of 1, 2, 4 and 8 cell staged embryos were 8.1, 1.4, 1.6 and 1.0 in control, but the numbers of 1-cell stage in PAPh treatment group was 24.2, which was significantly higher than those of treatment groups (P<0.05). Therefore, these data indicated that hormonal treatment, especially PAPh, enhanced the developments of follicles, corpus lutea and embryos and increased the collection rate of the 1-cell stage embryos to introduce of foreign genes.

• 한국동물생명공학회지
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• 제35권4호
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• pp.323-328
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• 2020

Direct injection of genome editing tools such as CRISPR/Cas9 system into developing embryos has been widely used to generate genetically engineered pigs. The approach allows us to produce pigs carrying targeted modifications at high efficiency without having to apply somatic cell nuclear transfer. However, the targeted modifications during embryogenesis often result in mosaicism, which causes issues in phenotyping founder animals and establishing a group of pigs carrying intended modifications. This study was aimed to establish a genomic PCR and sequencing system of a single blastomere in the four-cell embryos to detect potential mosaicism. We performed genomic PCR in four individual blastomeres from four-cell embryos. We successfully amplified target genomic region from single blastomeres of 4-cell stage embryo by PCR. Sanger sequencing of the PCR amplicons obtained from the blastomeres suggested that PCR-based genotyping of single blastomere was a feasible method to determine mutation type generated by genome editing technology such as CRISPR/Cas9 in early stage embryos. In conclusion, we successfully genotyped single blastomeres in a single 4-cell stage embryo to detect potential mosaicism in porcine embryos. Our approach offers a simple platform that can be used to screen the prevalence of mosaicism from designed CRISPR/Cas9 systems.

• 한국가축번식학회지
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• 제12권2호
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• pp.91-96
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• 1988

A study was conducted to improve breeding efficiency of dairy and beef cows on Cheju Island. Milk progesterone and plasma progesterone profiles were determined by using radioimmunoassay techniques during the oestrus cycle, onset on oestrus and during the gestation period. Progesterone connections in milk were very low during the oestrus with a means value of 2.4ng/ml and 4.6ng/ml at the onset of oestrus but gradually declined at 4 to 9 hr after oestrus. In the cyclic cow, milk progesterone concentraitons in the early part of the cycle, ranged from 3 to 5ng/ml and increased from 6.0 to 11.6ng/ml on day 13 to 21 of cycle. Milk progesterone concentrations of pregnancy cows ranged from 5.0 to 40ng/ml during the gestation and grom basal line to 3.5ng/ml for nonpregnant cows. Plasma progesterone concentrations of beef during pregnancy period ranged form 3.0 to 33ng/ml and basal levels ranged from 0 to less than 2.5ng/ml for non-pregnant beef cows.

• 한국동물생명공학회지
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• 제38권3호
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• pp.121-130
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• 2023

Background: Coating a culture plate with molecules that aid in cell adhesion is a technique widely used to produce animal cell cultures. Extracellular matrix (ECM) is known for its efficiency in promoting adhesion, survival, and proliferation of adherent cells. Gelatin, a cost-effective type of ECM, is widely used in animal cell cultures including feeder-free embryonic stem (ES) cells. However, the optimal concentration of gelatin is a point of debate among researchers, with no studies having established the optimal gelatin concentration. Methods: In this study, we coated plastic plates with gelatin in a concentration-dependent manner and assessed Young's modulus using atomic force microscopy (AFM) to investigate the microstructure of the surface of each plastic plate. The adhesion, proliferation, and differentiation of the ESCs were compared and analyzed revealing differences in surface microstructure dependent on coating concentration. Results: According to AFM analysis, there was a clear difference in the microstructure of the surface according to the presence or absence of the gelatin coating, and it was confirmed that there was no difference at a concentration of 0.5% or more. ES cell also confirmed the difference in cell adhesion, proliferation, and differentiation according to the presence or absence of gelatin coating, and also it showed no difference over the concentration of 0.5%. Conclusions: The optimum gelatin-coating for the maintenance and differentiation of ES cells is 0.5%, and the gelatin concentration-mediated microenvironment and ES cell signaling are closely correlated.

• 한국동물생명공학회지
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• 제39권2호
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• pp.114-120
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• 2024

Background: The Hanwoo industry must develop technologies that can increase the production of preferred cuts to match changing consumer trends. In this study, we aimed to estimate the genetic parameters for carcass traits (carcass weight, eye muscle area, back fat thickness, and intramuscular fat) and primal cut traits (tenderloin, loin, strip loin, neck, clod, top round, bottom round, brisket, shank, and rib) in a Hanwoo population to obtain basic data for improving primal cut productivity. Methods: Data from 1,905 Hanwoo steers, including carcass traits and primal cut weights, were collected. Genetic parameters were estimated using REMLF90 in a multi-trait analysis. Results: High heritability was found for carcass weight (0.52) and strip loin yield (0.63). Genetic correlations between carcass weight and primal cut weights ranged from 0.52 to 0.93. Conclusions: This study demonstrates the significant potential for genetic improvement in Hanwoo cattle through selective breeding, particularly for traits with high heritability and genetic correlations. These findings provide crucial insights into optimizing breeding programs to improve Hanwoo cattle production efficiency.

• International Journal of Internet, Broadcasting and Communication
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• 제7권2호
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• pp.16-27
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• 2015

Holography technology which was developed by Dennis Gabor (1900~1979) in 1948 is a technology to record wave planes of actual 3D objects. It is known as the only technology which can express 3D information most perfectly close to human-friendly. Holography technology is widely used in advertisement, architecture and arts as well as science technology areas. Especially, digital holographic print which is an applied area is greatly used in military map, architecture map and cultural asset restoration by printing and reproducing 3D information. Holography is realized by recording and reproducing the amplitude and phase information on high resolution film using coherent light like laser. Recording materials for digital holographic printer are silver halide, photoresist and photopolymer. Because the materials have different diffraction efficiency according to film characteristics of each manufacturer, appropriate guide lines should be suggested through efficiency analysis of each film. This paper suggests appropriate guide lines through the diffraction efficiency measurement of silver halide which is a holographic printer recording medium. And the objective of this study is to suggest appropriate guide lines through diffraction efficiency analysis of Ultimate 08-C and PFG-03C which are commercially used. The experiment was prepared by self-diffraction efficiency system which measures the strength with the defector by penetrating RGB recording medium and concentrating diffracted beams through collimating lens. The experiment showed Geola's PFG-03C which is a silver halide for full color has price/performance advantage in optical hologram recording, but recording angles and reproduction angles are irregular for digital holographic printer recording. Ultimate's Ultimate08-C for full color shows its diffraction efficiency is relatively stable and high according to recording angles and laser wavelength.

• Asian-Australasian Journal of Animal Sciences
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• 제30권5호
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• pp.666-671
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• 2017

Objective: Aim of the current comparative study was to evaluate production outputs, reproduction efficiency and functional traits in dual-purpose Fleckvieh and Braunvieh cows, reared under temperate European conditions. Methods: A data-set from 414 Fleckvieh and 42 Braunvieh cows and 799 lactations was analysed. ID tag number, milk yield per milking session, number of steps/interval and milk conductivity were recorded and collected daily using AfiMilk 3.076 A-DU software (Afimilk Ltd., Kibbutz, Israel). Production and milk quality data were taken from the results of the official performance recordings and the reproductive outputs of cows were recorded by the research stations veterinarians. Comparisons between the two genotypes were carried out using the one way analysis of variance protocol, with categorical factor being considered the breed of cows. All the statistical inferences were carried out using Statistica software (StatSoft Inc., Tulsa, OK, USA). Results: Fleckvieh cows significantly outperformed ($p{\leq}0.05$) the Braunvieh herd, with average milk yields of $5,252.1{\pm}35.79kg$ and $4,897.6{\pm}128.94kg$, respectively. Age at first calving was significantly ($p{\leq}0.01$) influenced by the breed, with Fleckvieh heifers being more precocious ($32.8{\pm}0.29mo$) compared to those of Braunvieh breed ($35.7{\pm}0.84mo$). Reproduction efficiency as defined by the number of inseminations per gestation, calving interval, dystocia, days dry and days open, was not influenced by genotype (p>0.05). Incidences of sub-clinical mastitis, clinical mastitis, lameness and abortions were not influenced by the breed factor (p>0.05). Stay-ability of cows was significantly ($p{\leq}0.001$) influenced by genotype, with Braunvieh cows having an average age at culling of $117.88{\pm}11.78$ months compared to $90.88{\pm}2.89$ months in Fleckvieh. Conclusion: Overall, results have shown that genotype significantly influenced milk yield, age at first calving and longevity.