• Proceedings of the Microbiological Society of Korea Conference
• /
• 2002.10a
• /
• pp.180-182
• /
• 2002

Substantial genomic diversity has been expected among clinical isolates of H. pylori. We have suggested that the two complete H. pylori genomes already sequenced may be insufficient for providing a discriminatory tool for typing clinical isolates as well as an insight into the genomic diversity, which enable to establish strategy for control of H. pylori infection. In this study, we determine the nucleotide sequence of the entire genome of Korean strain 51 and compare it with two reported genomic sequences to suggest validity for extensive genomic sequencing of H. pylori. The genome of H. pylori 51 consists of a circular chromosome with a size of 1,591,297 bp, which is corresponding to $95.4\%\;and\;96.8\%$ of the 26695 and J99 chromosome length, respectively. We predict that there are 1,454 open reading frames (ORFs) in 51, representing $91.4\%\;and\;97.2\%$ of the reported numbers of ORF of 26695 and J99, respectively. In contrast to 26695 and J99 that have 123 and 65 strain-specific genes, respectively, of the 1,454 genes, only 39 genes are unique to 51. Differences in genomic organization between 51 and each foreign strain were greater than between 2 foreign strains in pair wise entire sequence alignments by BLASTN. Particularly, the extent of genomic rearrangement observed between 51 and 26695 is higher than between 51 and J99. Multiple sequence alignment of orthologous genes among 3 strains showed that 51 is genetically closer to 26695 rather than J99. Phylogenetic analysis of nonsynonymous and synonymous mutation indicated J99 has the longest branch length in the unrooted phylogenetic tree, suggesting that J99 has higher mutation rate than the other 2 strains.

• Horticultural Science & Technology
• /
• v.31 no.5
• /
• pp.598-606
• /
• 2013

Anther is the major organ of flower in responsible to reproduction and outward appearance. From anther-specific cDNA library of Lilium Oriental Hybrid 'Acapulco', 2000 expressed sequence tags were selected randomly. Differential slot blot analysis with cDNA probes from the anther and leaf was used to get anther-expressed clone and 570 non-redundant ESTs were obtained and sequenced. Compared to the GenBank database using BLASTX algorithm, 191 clones showed significant similarity but others (66.5%) did not measured to known sequence. Functional categories according to gene ontology (GO) annotation included sequence representing a significant portion of protein in cell and cell part respectively. A transcriptional analysis at 7 different organs and developmental stage was performed using northern blot with thirty ESTs as putative anther specific gene. This report suggest that selection of anther expressed clone using differential slot blot was considered as very effective tool and our current study can provide fundamental information on the lily anther including pollen furthermore.

• Journal of Ginseng Culture
• /
• v.3
• /
• pp.1-10
• /
• 2021

Panax ginseng (Ginseng or Korean ginseng) is one of the most important medicinal herbs in the world. We made a high-quality whole genome sequence of P. ginseng using 'Chunpoong' cultivar, which is the first cultivar registered in Korea Seed and Variety Service (KSVS) with relatively similar genotypes and superior phenotypes, representing approximately 3 Gbp and 60,000 genes. Genome sequence analyses of P. ginseng and related speciesrevealed the origin of Korean ginseng and the ecological adaptation of 18 Panax species around the world. Korean ginseng and American ginseng (P. quinquefolius) are tetraploid species having 24 chromosome pairs, while the other 16 species are diploid species with 12 chromosome pairs. Panax and Aralia are the closest genera belonging to the Araliaceae family that diverged approximately 8 million years ago (MYA). All Panax species evolved as shade plants adapting to cool climates and low light conditions under the canopy of deep forests from Southeast Asia such as Vietnam to Northeast Asia such as Russia approximately 6 MYA. However, through recurrent ice ages and global warming, most diploid Panax species disappeared due to the freezing winter, while tetraploid P. ginseng may have appeared by allotetraploidization, which contributed to the adaptation to cold temperaturesin Northeast Asian countries including the Korea peninsula approximately 2 MYA. American ginseng evolved by the adaptation of P. ginseng in Northeast America after the intercontinental migration 1 MYA. Meanwhile, most of diploid Panax species survived in high-altitude mountains over 1,600 meters in Southeast Asia because they could not endure the hot temperature and freezing cold. The genome sequence provides good basisto unveil the origin and evolution of ginseng and also supports practical gene chips which is useful for breeding and the ginseng industry.

• 한국HCI학회:학술대회논문집
• /
• 2008.02a
• /
• pp.200-204
• /
• 2008

In this paper, to solve localization problem for out-door navigation of robotic vehicles, a particle filter based 3D object recognition framework that can estimate the pose of a building or its entrance is presented. A particle filter framework of multiple evidence fusion and model matching in a sequence of images is presented for robust recognition and pose estimation of 3D objects. The proposed approach features 1) the automatic selection and collection of an optimal set of evidences 2) the derivation of multiple interpretations, as particles representing possible object poses in 3D space, and the assignment of their probabilities based on matching the object model with evidences, and 3) the particle filtering of interpretations in time with the additional evidences obtained from a sequence of images. The proposed approach has been validated by the stereo-camera based experimentation of 3D object recognition and pose estimation, where a combination of photometric and geometric features are used for evidences.

• Research in Community and Public Health Nursing
• /
• v.7 no.2
• /
• pp.400-409
• /
• 1996

To investigate industrial fatigue and low back pain, the questionaire survey for subjective symptoms of fatigue and low back pain was carried out among 591 male workers aged 20-55 employed in an automobile industry in Korea. Workers participated to this study were divided into low back pain group(LBP) and control group, according to the self-reports by written questionaires. The subjective sysptoms of fatigue comprised three groups of 10 items each, representing dullness and sleepiness(level of cerebral activation), difficulty in concentration(level of motivation) and bodily projection of fatigue. The resultant data were processed for $\chi^2-test$, t-test and a pearson's correlation coefficient to confirm the relationships. The results were as follows: 1. 30 items of fatigue subjective symptoms exeptone item, 'lack in perseverance', were directly associated with low back pain. 2. The percentage of fatigue complaint were sig nificantly higher in LBP group. 3. Of the 30 items of fatigue subjective symptoms, the highest percentage was accounted for 'eye strain'(27.9%), followed by 'whole body feels tired' and 'legs feel heavy'(22.9%), 'feel like lying'(21.4%), 'feel a pain in the low back'(18.7%), 'feel drowsy'(16.4%) and 'feel stiffness in the neck or the shoulders'(16.2%) in the order of sequence. 4. The average weighted score for the first group of fatigue items(dullness and sleepiness) was the largest among three groups and was followed by the second group(difficulty in concentration) and the third group(bodily projection of fatigue) in the order of sequence, suggesting the heavier shift work stress of the workers. 5. In the groups of the aged 30-40, work duration of 5-7yrs, heavy work amount and irregular work speed, significant high fatigue complaints were revealed in terms of eye strain, whole body feels tired, legs feel heavy, feel like lying, feel a pain in the low back and feel stiffness in the neck or the shoulders. 6. A significant negative correlations were shown between age, work duration and eleven subjective symptoms while positive reciprocal correlations were shown between eleven items with one another.

• Journal of Aquaculture
• /
• v.16 no.2
• /
• pp.110-117
• /
• 2003

We have cloned and sequenced the cDNA encoding growth hormone (GH) from pituitary poly(A)$^{+}$ RNA of red-spotted grouper (Epinephelus akaara). The cDNA of red-spotted grouper GH is 883 base pairs (bp) consisting of 21 bp of 5'untranslated region (UTR), 615 Up of an open reading frame (ORF) and 247 Up of 3'UTR. The polyadenylation signal, AATAAA, was 20 bp upsteam of polyadenylation site. Based on the nucleotide sequences, the deduced putative polypeptide contains 204 amino acids (aa), representing 17 aa of a signal and 187 aa of a mature polypeptide. The putative GH cDNA encodes a polypeptide with four cysteine residues and only one N-gly- cosylation site. Comparative sequence alignment shows that red-spotted grouper GH exhibits high similarity with its corresponding other Perciformes species GH cDNAs.

• Journal of Preventive Medicine and Public Health
• /
• v.24 no.3 s.35
• /
• pp.305-313
• /
• 1991

In order to investigate industrial fatigue due to visual display terminal (VDT) work of banking operations the questionnaire survey for subjective symptoms of fatigue was carried out on 470 bank clerks who had been engaged in VDT work for various length of work hours. The questionnaires comprised three groups of 10 items each, representing dullness and sleepiness (level of cerebral activation), difficulty in concentration (level of motivation) and bodily projection of fatigue. The results were as follows : 1. Of the 30 items of questionnaires, the highest percentage was accounted for by 'eye strain' (51.5% ), followed by 'feel stiffness in the neck or the shoulders'(33.4%), 'feel a pain in the low back'(26.8%), 'whole body feels tired'(19.6%) and 'feel headache'(17.9%) in the order of sequence. 2. The average weighted score for the first group of questionnaire items (dullness and sleepiness) was the largest among three groups and was followed by 'the third cup (bodily projection of fatigue) and the second item group (difficulty in concentration) in the order of sequence, suggesting the heavier mental stress of VDT work in banking operations rather than physical burden. 3. In terms of the age and sex of workers, work duration and VDT work percentage, the difference in average weighted score was noted only between sex, the score of female being larger than that of male. 4. The complaint rates of subjective symptoms showed close associations with the subjective optimums of room temperature, ventilation, illumination and noise level. 5. The significant correlation was showed between age, work duration and item of 'whole body feels tired', between VDT work percentage and items of 'eye strain' and 'feel stiffness in the neck or shoulders' and between all items of subjective symptoms.

• The Plant Pathology Journal
• /
• v.29 no.4
• /
• pp.357-363
• /
• 2013

The fungus Venturia nashicola is the causal agent of scab on Asian pears. For the rapid and reliable identification as well as sensitive detection of V. nashicola, a PCR-based technique was developed. DNA fingerprints of three closely related species, V. nashicola, V. pirina, and V. inaequalis, were obtained by random amplified polymorphic DNA (RAPD) analysis. Two RAPD markers specific to V. nashicola were identified by PCR, after which two pairs of sequence characterized amplified region (SCAR) primers were designed from the nucleotide sequences of the markers. The SCAR primer pairs, designated as D12F/D12R and E11F/E11R, amplified 535-bp and 525-bp DNA fragments, respectively, only from genomic DNA of V. nashicola. The specificity of the primer sets was tested on strains representing three species of Venturia and 20 fungal plant pathogens. The nested PCR primer pair specific to V. nashicola was developed based on the sequence of the species-specific 525-bp DNA fragment amplified by primer set E11F/E11R. The internal primer pair Na11F/Na11R amplified a 235-bp fragment from V. nashicola, but not from any other fungal species tested. The nested PCR assay was sensitive enough to detect the specific fragment in 50 fg of V. nashicola DNA.

• Korean Journal of Occupational Health Nursing
• /
• v.8 no.2
• /
• pp.162-168
• /
• 1999

To investigate industrial fatigue and low back pain, the questionaire survey for subjective symptoms of fatigue and low back pain was carried out 305 nurses in general hospital in Korea. Nurses participated to this study were divided into low back pain group(LBP) and control group, according to the self-reports by written questionaires. The subjective sysptoms of fatigue comprised three groups of 10 items each, representing dullness and sleepiness(level of cerebral activation), difficulty in concentration(level of motivation) and bodily projection of fatigue. The resultant data were processed for $x^2$-test, t-test to confirm the associations. The results were as follows: 1. 28 items of fatigue subjective symptoms exept two item, 'feel thirsty and 'feel ill', were directly associated with low back pain. 2. The percentage of fatigue complaint were significantly higher in LBP group than control. 3. Of the 30 items of fatigue subjective symptoms, the highest percentage was accounted for 'legs feel heavy', 'eye strain', 'feel stiffness in the neck or the shoulders' followed by 'feel like lying', 'whole body feels tired', 'feel a pain in the low back', 'feel drowsy' and in the order of sequence. 4. The average weighted score for the first group of fatigue items(dullness and sleepiness) was the largest among three groups and was followed by the second group(difficulty in concentration) and the third group (bodily projection of fatigue) in the order of sequence, suggesting the heavier mental and shift work stress of the female workers.

• KSCI Review
• /
• v.14 no.1
• /
• pp.241-248
• /
• 2006

The recognition of a relatively big and rarely movable object. such as refrigerator and air conditioner, etc. is necessary because these objects can be crucial global stable features of Simultaneous Localization and Map building(SLAM) in the indoor environment. In this paper. we propose a novel method to recognize these big objects using a sequence of 3D scenes. The particles representing an object to be recognized are scattered to the environment and then the probability of each particles is calculated by the matching test with 3D lines of the environment. Based on the probability and degree of convergence of particles, we can recognize the object in the environment and the pose of object is also estimated. The experimental results show the feasibility of incremental object recognition based on particle filtering and the application to SLAM