• Journal of Microbiology
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• v.33 no.3
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• pp.252-259
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• 1995

The PCR was employed to detect and characterize the bovine immunodeficiency-like virus (BIV), which is a newly recognized member of the I entivirinae of the retroviruses. Degenerate primers representing the conserved regions in the pol genes of the Lentivirinae, were used to detect proviral DNA obtained from the bovine embryonic spleen cell cultures infected with BIV. The PCR amplified DNA fragment was molecularly cloned and sequenced. The BIV DNA fragment contained a sequence identical to that reported by Garvey et al. (Garvey et al., 1990. Virology, 175, 391-409). With the degenerate primers, peripheral blood mononuclear cells (PBMCs) of sick cattle and cells cultured with BIV were tested to determine genetic variation of BIV pol conserved sequence. We found the sequence heterogeneity within cultures and most variations occurred at the third base of codons that would not lead to amino acid substitutions. Another change was GAG (Glu) to AAG (Lys) within the BIV isolates. Interestingly, the altered sequence is also found in other lentiviruses such as HIV-2, SIV mac, CAEV and EIAV.

• Journal of Information Management
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• v.2 no.4
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• pp.6-8
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• 1964

This first article in a series of descriptions of devices and methods in the field of automated information processing depicts two machines representing a. certain type of documentation systems which use micro-images and coding of documents in an unalterable sequence, namely on 16mm filmrolls.

• Journal of Aquaculture
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• v.10 no.4
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• pp.403-407
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• 1997

Intraspecific sequence variation was detected by polymerase chain reaction (PCR) and direct sequencing of a 350-nucleotide region of the mitochondrial 12S rRNA gene of two natural populations (Han River and Nakdong River) and one hatchery stock (Jinhae Inland Fisheries Institute) of local strain common carp, one Israeli strain of common carp stock from Pukyong National University (PKU), and one hybrid between Israeli strain of common carp female and local strain common carp male from PKU stock. There is little variation in 350 bases of the mitochondrial 12S rRNA gene sequences among 2 natural and 1 hatchery local strain common carp populatins, representing abut 7 to 20 nucleotide differences (less than 6%). The sequence of specimens from Han River was more similar to that from Nakdong River (identity=98.0%) than to that from Jinhae Inland Fisheries Institute (identity=96.3%). Sequence variation between Israeli strain and wild local strain common carp was higher than the variation within natural stocks. The level of variation was ranged from 15.7 to 17.7%. The hybrid showed very similar nucleotide4 sequence of 12S rRNA gene to the sequence of Israeli strain with the identity of 98.9%.

• The Transactions of the Korea Information Processing Society
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• v.5 no.5
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• pp.1204-1215
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• 1998

In this paper, we propose a scenario representing method, a specification language, and a verification technique for OARTS(Object based Approach for Real-Time Systems). As well as the general modeling method(event trace diagram), this study includes a specification language and a verification technique because there was no study about methodological level technique for scenario development as yet. Centering around the synchronization problem of transition of external modules which are the communication interfaces based on the objects, we lay stress on the representation of sequence of external events and internal action transitions. From the results of practical experiences, it has been ascertained that the proposed method reflect well the requirements in the analysis step, and its validity of the representation has been identified by a conceptual verifier. We support that it can serve as an analyzing tool for representing a general real-time scenarios also.

• Journal of fish pathology
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• v.22 no.3
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• pp.383-389
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• 2009

Analysis of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and development of resources useful for functional genomics studies. As part of studies on the immune system of olive flounder, a total of 251 EST sequences from gill cDNA library were generated to identify and characterize important genes in the immune machanisms of olive flounder. Of the 251 clones, 126 clones (50.2%) were identified as orthologues of known genes from olive flounder and other organisms. Among the 126 EST clones, 16 clones (12.7%) were representing 9 unique genes identified as homologous to the previously reported olive flounder ESTs, 100 clones (79.4%) representing 103unique genes were identified as orthologs of known genes from other organisms. We also identified several kinds of immune associated proteins, indicating EST as a powerful method for identifying immune related genes of fish as well as identifying novel genes. Further studies using cDNA microarrays are needed to identify the differentially expressed transcripts after disease infection.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.36 no.4
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• pp.130-137
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• 2013

In this paper, we consider a two-agent scheduling with sequence-dependent exponential learning effects consideration, where two agents A and B have to share a single machine for processing their jobs. The objective function for agent A is to minimize the total completion time of jobs for agent A subject to a given upper bound on the objective function of agent B, representing the makespan of jobs for agent B. By assuming that the learning ratios for all jobs are the same, we suggest an enumeration-based backward allocation scheduling for finding an optimal solution and exemplify it by using a small numerical example. This problem has various applications in production systems as well as in operations management.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.115.1-115
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• 2003

Post-harvest decay, caused by Penicillium spp. is a serious problem of fruits worldwide. Morphological characteristics and molecular markers were used to characterize 22 Penicillium isolates from apples, 18 isolates from pears, 60 from oranges and 18 from grapes and 23reference isolates representing related Penicillium spp. to assess their diversity and resolve their taxonomy. Based on morphological and physiological characteristics, the isolates were grouped as identical or very similar to P. digitatum, P. italicum, P. ulaiense or very similar to P. crustosum, P. expansum, P. solitum and unidentified Penicillium spp. Based on sequence comparisons of ITS region, variable site were presented within and among the species, but there variation were not correlated with the species. Cluster analyses of AP-PCR fragment patterns using UP and L45 primer and the -tubulin gene sequence, the Penicillium species were segregated into distinct groups. Particularly. the -tubulin partial sequence data provided support for species concepts based on morphological and physiological characteristics.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2016.05a
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• pp.199-200
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• 2016

Accurate fire diagnoses are needed to properly repair and strengthen buildings affected by fire. The current diagnosis method of fire takes time and is ineffective. In previous research, Melting point temperature of each sequence to grasp easily the temperature of the concrete up to 200 ~ 600 ℃ was to estimate the temperature by utilizing a different sequence representing material.But In the form of conventional hydrothermal temperature prediction simple measuring device, it is difficult in the future buried in application to the construction site, there is a problem of damage when concrete pouring, and only the extension of life measured by the zinc has a problem does not distinguish between 400 ℃ and 500 ℃. Therefore this study is conducted by utilizing a titanium metal changes the color depending on the temperature to check for the applicability of the simple apparatus for measuring the temperature prediction sequence.

• Journal of Microbiology and Biotechnology
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• v.9 no.4
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• pp.457-463
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• 1999

The physical map of bacteriophage $\PhiFC1$ DNA was constructed with the restriction endonucleases SalI, BamHI, EcoRI, XbaI, and AvaI. The 40.5-kb DNA restriction map is shown to be circularly permuted representing the headful packaging mechanism of the phage. The DNA restriction fragments containing the packaging initiation site(pac) was localized on the restriction map and the nucleotide sequences of the region were analyzed. Four open reading frames (ORFs), following one another with the same orientation, were found at the region. The 2nd ORF (ORF-ts) has significant amino acid sequence homologies to the previously known terminase small subunits of other bacteriophages. The putative terminase small subunit gene has a presumptive NTP-hydrolysis motif and a helix-turn-helix motif. The cleavage site for the first round of packaging was found to be located at the coding sequence of the putative terminase small subunit gene. The fourth ORF, even if partially sequenced, has a good amino acid sequence homology to the portal vertex proteins of other bacteriophages representing the evolutionarily conserved arrangements of genes near the pac site of this bacteriophage, $\PhiFC1$.

• The Transactions of the Korea Information Processing Society
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• v.7 no.2
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• pp.529-535
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• 2000

In this paper, we propose a new method of designing the vector quantizer which is robustness to coding results and independent of statistical characteristics of an input image in wavelet transformed image coding processes. The most critical drawback of a conventional vector quantizer is the degradation of coding capability resulted from the discordance between quantizer objective image and statistical characteristics of training sequence which is for generating representing vector. In order to resolve the problem of conventional methods, we use independent random-variables and pseudo image to which image correlation and edge component were added, as a training sequence for generating representing vector. We have done a computer simulation in order to compare coding capability between a vector quantizer designed by the proposed method and one with the conventional method using real image as same as that is objective to coding of training sequence used in codebook generation. The results show the superiority of the proposed vector quantizer method at the aspect of coding capability compared to conventional one. They also clarify the problems of conventional methods.