• Korean Journal of Agricultural Science
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• v.43 no.2
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• pp.221-231
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• 2016

Microsatellites, which are sequences of repetitive short nucleotides, are abundant in the genome and have relatively many alleles at a locus. Hence, microsatellite markers are used in various research areas such as medicine, agriculture, and biology. Thanks to recent advanced techniques and databases associated with microsatellite marker development, foreign research relying on microsatellite markers is increasing in various study areas. In this study, by analyzing microsatellites-related articles published during 2000-2014 from eight Korean national journals representing zoology, botany, genetics, ecology and environmental science, breeding science, and forest science ('Animal Cells and Systems', 'Journal of Plant Biology', 'Genes and Genomics', 'Korean Society of Environment and Ecology', 'Korean Journal of Breeding Science', 'Journal of Agricultural Science, Chungnam National University', 'Journal of Korean Forest Society' and 'Forest Science and Technology'), we found that the number of articles and diversity of study subjects and objects have increased considerably. However, there are fewer applications of microsatellites in the national forest science area. During 2000-2014 in 'Journal of Korean Forest Society', the percentage of articles dealing with microsatellite markers was found to be the lowest with 4.2% among articles focusing on PCR-based markers including RAPD, AFLP, and ISSR. However, in 'Canadian Journal of Forest Research' and 'Forest Ecology and Management', microsatellite marker articles were represented at their highest with 69.2% and 76.2%, respectively. Given the advantages of microsatellite markers, the publication of research papers using microsatellites should be increased in Korean forest science journals to the level of studies published in prominent international journals.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.6
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• pp.876-887
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• 2015

Dicer, an ribonuclease type III type endonuclease, is the key enzyme involved in biogenesis of microRNAs (miRNAs) and small interfering RNAs (siRNAs), and thus plays a critical role in RNA interference through post transcriptional regulation of gene expression. This enzyme has not been well studied in the Indian water buffalo, an important species known for disease resistance and high milk production. In this study, the primary coding sequence (5,778 bp) of bubaline dicer (GenBank: AB969677.1) was determined and the bubaline Dicer1 biocomputationally characterized to determine the phylogenetic signature among higher eukaryotes. The evolutionary tree revealed that all the transcript variants of Dicer1 belonging to a specific species were within the same node and the sequences belonging to primates, rodents and lagomorphs, avians and reptiles formed independent clusters. The bubaline dicer1 is closely related to that of cattle and other ruminants and significantly divergent from dicer of lower species such as tapeworm, sea urchin and fruit fly. Evolutionary divergence analysis conducted using MEGA6 software indicated that dicer has undergone purifying selection over the time. Seventeen divergent sequences, representing each of the families/taxa were selected to study the specific regions of positive vis-$\grave{a}$-vis negative selection using different models like single likelihood ancestor counting, fixed effects likelihood, and random effects likelihood of Datamonkey server. Comparative analysis of the domain structure revealed that Dicer1 is conserved across mammalian species while variation both in terms of length of Dicer enzyme and presence or absence of domain is evident in the lower organisms.

• KIPS Transactions on Software and Data Engineering
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• v.9 no.2
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• pp.69-76
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• 2020

In this paper, we introduce a method to visualize the contrast diffusion patterns and the dynamic vascular patterns in a contrast-enhanced ultrasound image sequence. We present an imaging technique to visualize parameters such as contrast arrival time, peak intensity time, and contrast decay time in contrast-enhanced ultrasound data. The contrast flow pattern and its velocity are important for characterizing focal liver lesions. We propose a method for representing the contrast diffusion patterns as an image. In the methods, respiratory motion may degrade the accuracy of the parametric images. Therefore, we present a respiratory motion tracking technique that uses dynamic weights and a momentum factor with respect to the respiration cycle. Through the experiment using 72 CEUS data sets, we show that the proposed method makes it possible to overcome the limitation of analysis by the naked eye and improves the reliability of the parametric images by compensating for respiratory motion in contrast-enhanced ultrasonography.

• The Korean Journal of Quaternary Research
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• v.3 no.1
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• pp.55-68
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• 1989

This paper presents examples of the computer-aided zonation and correlation of pollen data from the Late-glacial to Holocene stratigraphic sequences at four sites in central Iowa, U.S.A. Spearman's rank correlation coefficient matrix and first four components of Principal components analysis plotted in a stratigraphic order are combined to provide an excellent zonation of the pollen data at each site. Correlation of the four pollen sequences are conducted by Principal components analysis of the data sets combined in one. The first and second principal components successfully provide correlation lines that match fairly closely the zone boundaries of each pollen sequence. The third and fourth components, in contrast, are greatly different from site to site, representing the unique pollen assemblages at each site.

• Journal of Microbiology and Biotechnology
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• v.16 no.2
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• pp.193-199
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• 2006

Halotolerant spore-forming Gram-positive bacteria were isolated from the root, rhizosphere, and non-rhizosphere soil of Blutaparon portulacoides. The different isolates were characterized genetically using an amplified ribosomal DNA restriction analysis (ARDRA), and phenotypically based on their colonial morphology, physiology, and nutritional requirements. Three different 16S rRNA gene-based genotypes were observed at a 100% similarity using the enzymes HinfI, MspI, and RsaI, and the phenotypic results also followed the ARDRA groupings. Selected strains, representing the different ARDRA groups, were analyzed by 16S rDNA sequencing, and members of the genera Halobaeillus, Virgibacillus, and Oceanobacillus were found. Two isolates showed low 16S rDNA sequence similarities with the closest related species of Halobacillus, indicating the presence of new species among the isolates. The majority of the strains isolated in this study seemed to belong to the species O. iheyensis and were compared using an AP-PCR to determine whether they had a clonal origin or not. Different patterns allowed the grouping of the strains according to Pearson's coefficient, and the resulting dendrogram revealed the formation of two main clusters, denoted as A and B. All the strains isolated from the soil were grouped into cluster A, whereas cluster B was exclusively composed of the strains associated with the B. portulacoides roots. This is the first report on the isolation and characterization of halotolerant spore-forming Gram-positive bacteria that coexist with B. portulacoides. As such, these new strains may be a potential source for the discovery of bioactive compounds with industrial value.

• Journal of Broadcast Engineering
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• v.4 no.2
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• pp.87-102
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• 1999

We propose an algorithm for augmenting a real video sequence with views of graphics ojbects without metric calibration of the video camera by representing the motion of the video camera in projective space. We define a virtual camera, through which views of graphics objects are generated. attached to the real camera by specifying image locations of the world coordinate system of the virtual world. The virtual camera is decomposed into calibration and motion components in order to make full use of graphics tools. The projective motion of the real camera recovered from image matches has a function of transferring the virtual camera and makes the virtual camera move according to the motion of the real camera. The virtual camera also follows the change of the internal parameters of the real camera. This paper shows theoretical and experimental results of our application of non-metric vision to augmented reality.

• Journal of the Korean Wood Science and Technology
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• v.34 no.1
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• pp.68-78
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• 2006

The purpose of this study was to estimate the identification of thermophilic hacterial with fermentation of pig manure. To identify the characters of thermophilic bacteria related to fermentation at a high temperature condition, we selected 28 different kinds of original settling thermophilic bacteria that were sampled at different 23 areas. They were distributed 1$1{\times}10^5{\sim}10^8CFU$ at medium and the enzyme activity at $55^{\circ}C$ incubation condition, especially cellulase and a-amylase, were higher than those of $30^{\circ}C$. Partial sequencing data for 165 rDNA region were obtained from 28 samples representing 15 different genera. Bacillus subcilis, one of those bacteria, has endodermic spores at high fermented condition.

• Korean Journal of Environmental Agriculture
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• v.29 no.4
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• pp.388-395
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• 2010

In order to decrease level of an organophosphorus fungicide, tolclofos-methyl, from in situ ginseng cultivating soil, we isolated a tolclofos-methyl degrading bacteria from ginseng cultivating soil samples. The bacterial strain removed tolclofos-methyl around 95% after 3 days incubation with complete liquid media. The strain was identified as Sphingomonas sp. by 16S rDNA sequence comparison, and designated as Sphingomonas sp. 224. Through the GC-MS analysis, Sphingomonas sp. 224 was proposed to have an initiative degradation pathway generating the metabolite such as 2,6-dichloro-4-methyl phenol compound from tolclofos-methyl. In addition, Sphingomonas sp. 224 was confirmed representing the effective degrading capability to tolclofosmethyl in situ soil.

• The Transactions of the Korea Information Processing Society
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• v.5 no.12
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• pp.3063-3076
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• 1998

To facilitate presentations of various media and transfer their implicit meaning efficiently, needed are effective development techniques for representing and manipulation temporal and spatial informations between multimedia data. Especially, these emporal and spatial informationsl are so deeply related with each other that it is required to have the integrated mehod for their representation. This paper suggests the Presentation Synchronization Trdd(PST) which represents their temporal and spatial informationsl and the efficient presentation model as traversing this PST. In this model, without knowing the specific user's interface or any script languages, the user can write the presentation scenario only based on allen's tecporal specification. This model has a characteristic of allowing user's inputs that may dynamically modify the presentation sequence in presentation time. As through the renmining steps of consistency checking for their relations. PST generation, and PST traversing step, this model provides users with simple and effective presentation of multimedial data by having them be presented auton\matically.

• BMB Reports
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• v.40 no.1
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• pp.95-99
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• 2007

Bovine coding region single nucleotide polymorphisms located proximal to quantitative trait loci were identified to facilitate bovine QTL fine mapping research. A total of 692,763 bovine SNPs was extracted from 39,432 UniGene clusters, and 53,446 candidate SNPs were found to be a depth >3. In order to validate the in silico SNPs experimentally, 186 animals representing 14 breeds and 100 mixed breeds were analyzed. Genotyping of 40 randomly selected candidate SNPs revealed that 43% of these SNPs ranged in frequency from 0.009 to 0.498. To identify non-synonymous SNPs and to correct for possible frameshift errors in the ESTs at the predicted SNP positions, we designed a program that determines coding regions by protein-sequence referencing, and identified 17,735 nsSNPs. The SNPs and bovine quantitative traits loci informations were integrated into a bovine SNP data: BcSNPdb (http://snugenome.snu.ac.kr/BtcSNP/). Currently there are 43 different kinds of quantitative traits available. Thus, these SNPs would serve as valuable resources for exploiting genomic variation that influence economically and agriculturally important traits in cows.