• 생약학회지
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• 제50권3호
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• pp.165-174
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• 2019

Oregonin is known as a representative active substance of plants of the Alnus species. In this study, we will show the results of establishing a method for extracting trace amounts of useful substances in plants. Additionally, a variety of physiological activity studies were conducted with extracts containing high content of useful substances. In this study, anti-oxidant, anti-inflammatory and anti-allergic activities of supercritical fluid residue 60% EtOH extracts of Alnus sibirica (ASCFR) were investigated in vitro. To explore anti-oxidant and anti-inflammatory characteristics of supercritical extract, DPPH radical scavenging, and ABTS radical scavenging activity, inhibition of nitric oxide production in LPS-stimulated RAW 264.7 cells and ${\beta}$-hexosaminidase release in IgE-stimulated RBL-2H3 cells were examined, respectively. Results showed a concentration-dependent increase in DPPH and ABTS radical scavenging activity. Cells stimulated with LPS produced more nitric oxide than normal control cells. However, cells treated with supercritical fluid residue 60% EtOH extract decreased production in a concentration-dependent manner. In anti-allergic activity studies, ASCFR showed concentration-dependentanti-allergic activity. Based on results and strong antioxidant activity, ASCFR has potential as basic research materials for development of therapeutic supplements-based medicines or functional cosmetics related to chronic inflammatory skin immunity diseases.

• Nuclear Engineering and Technology
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• 제54권1호
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• pp.386-400
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• 2022

Seismic fragility analysis, a part of seismic probabilistic risk assessment (SPRA), is commonly used to establish the relationship between a representative property of earthquakes and the failure probability of a structure, component, or system. Current guidelines on the SPRA of nuclear power plants (NPPs) used worldwide mainly reflect the earthquake characteristics of the western United States. However, different earthquake characteristics may have a significant impact on the seismic fragility of a structure. Given the concern, this study aimed to investigate the effects of earthquake characteristics on the seismic fragility of concrete containments housing the OPR-1000 reactor. Earthquake time histories were created from 30 ground motions (including those of the 2016 Gyeongju earthquake) by spectral matching to the site-specific response spectrum of Hanbit nuclear power plants in South Korea. Fragility curves of the containment structure were determined under the linear response history analysis using a lumped-mass stick model and 30 ground motions, and were compared in terms of earthquake characteristics. The results showed that the median capacity and high confidence of low probability of failure (HCLPF) tended to highly depend on the sustained maximum acceleration (SMA), and increase when using the time histories which have lower SMA compared with the others.

• 한국초지조사료학회지
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• 제42권3호
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• pp.137-145
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• 2022

Aluminum (Al) is one of the major factors adversely affects crop growth and productivity in acidic soils. In this study, the effect of Al on plants in soil was investigated by comparing the protein expression profiles of alfalfa roots exposed to Al stress treatment. Two-week-old alfalfa seedlings were exposed to Al stress treatment at pH 4.0. Total protein was extracted from alfalfa root tissue and analyzed by two-dimensional gel electrophoresis combined with MALDI-TOF/TOF mass spectrometry. A total of 45 proteins differentially expressed in Al stress-treated alfalfa root tissues were identified, of which 28 were up-regulated and 17 were down-regulated. Of the differentially expressed proteins, 7 representative proteins were further confirmed for transcript accumulation by RT-PCR analysis. The identified proteins were involved in several functional categories including disease/defense (24%), energy (22%), protein destination (9%), metabolism (7%), transcription (5%), secondary metabolism (4%), and ambiguous classification (29%). The identification of key candidate genes induced by Al in alfalfa roots will be useful to elucidate the molecular mechanisms of Al stress tolerance in alfalfa plants.

• Journal of the Korean Wood Science and Technology
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• 제51권2호
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• pp.145-156
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• 2023

Many skin diseases are caused by microbial infections. Representative pathogenic fungus and bacterium that cause skin diseases are Candida albicans and Staphylococcus aureus, respectively. Malassezia pachydermatis is a fungus that causes animal skin diseases. In this study, we propose a method for removing pathogenic microorganisms from the skin using relatively safe edible herbal extracts. Herbal extracts were screened for skin health through the removal of pathogenic microorganisms, and combinations for effective utilization of the screened extracts were identified. In this study, among methanol extracts of 240 edible plants, C. albicans, S. aureus, and M. pachydermatis were killed by extracts of 10 plants: Acori Gramineri Rhizoma, Angelicae Tenuissimae Radix, Cinnamomi Cortex, Cinnamomi Ramulus, Impatientis Semen, Magnoliae Cortex, Moutan Cortex Radicis, Phellodendri Cortex, Scutellariae Radix, and Syzygii Flos. By evaluating the synergistic antifungal activities against C. albicans using all 45 possible combinations of these 10 extracts, five new synergistic antifungal combinations, Acori Gramineri Rhizoma with Magnoliae Cortex extracts, Acori Gramineri Rhizoma with Phellodendri Cortex extracts, Angelicae Tenuissimae Radix with Magnoliae Cortex extracts, Magnoliae Cortex with Phellodendri Cortex extracts, and Phellodendri Cortex with Syzygii Flos extracts, were identified. By utilizing the selected extracts and five combinations with synergistic antifungal effects, this work provides materials and methods to develop new and safe methods for treating candidiasis using natural products.

• 한국전통조경학회지
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• 제37권2호
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• pp.30-39
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• 2019

본 연구는 이어(李漁; 1611-1680)의 "한정우기(閑情偶寄)" "종식부(種植部)"에 수록된 68종 식물 중 상징적 의미와 독특한 완상 방식을 지닌 27종의 식물을 추출하여 고찰했다. 해석한 내용을 요약하면 다음과 같다. 첫째, 식물의 상징성이다. 1) 모란은 외적으로 꽃이 아름다울 뿐만 아니라 강직하고 정직한 특성을 갖기 때문에 화왕(花王)으로 삼는다. 모란과 겨룰 수 있는 꽃은 작약밖에 없으나 작약을 화상(花相)으로 비유한다. 이어는 작약도 마땅히 다섯 등급의 제후(諸侯) 대열에 속해야 한다고 했다. 2) 이어는 복사꽃과 동백꽃을 미인의 뺨으로 해당화, 재스민 꽃, 다화장미, 수선화, 개양귀비를 자태가 다양한 여인으로 비유하면서 이에 대한 사랑스러운 감정을 꽃에 투영하였다. 3) 이어는 연꽃을 꽃 중의 군자로, 황양목을 나무 중의 군자로 삼는다. 반대로 서향을 꽃 중의 소인(小人)으로 비유한다. 4) 감탕나무는 풍채가 있으나 절개를 과시하지 않는 까닭에 이어는 감탕나무를 은사로, 멀리서 바라본 능소화를 하늘 끝의 신선으로 비유한다. 둘째, 식물의 완상 방식이다. 1) 식물감상 시 보조 시설을 활용해야 한다. 야외에 나갈 때 천막을 휴대하야 하고, 정원에서 매화를 감상 시 종이 병풍을 활용해야 정취를 더욱 느낄 수 있다고 했다. 매화 이외에도 이어는 난초 옆에 감상할 수 있는 기물을 배치해야 더욱 다채롭게 식물을 향유할 수 있다고 했다. 2) 이어는 백일홍과 살구나무의 예를 들어 식물은 동물과 사람처럼 지각이 있다고 주장했다. 3) 오감으로 식물과 교류하는 것이 필요하다고 했고, 이는 버드나무와 합환을 통해 가능하다고 했다. 연꽃과 매괴의 예를 들어 식물은 관상 가치뿐만 아니라 실용 가치가 있다고 강조했다. 4) 사물의 비유로, 일반 사람들은 계관화를 '닭 벼슬'로 보는 반면에 이어는 하늘의 상서로운 구름과 같다고 했다. 종합하자면 본 연구는 이어가 식물에 의미를 부여하여 인간의 다감각적 세계를 식물에 투영해서 인격화하고 식물에 대한 그의 사랑을 더해 독특한 식물 향유 방식을 표출해 냈다는 것에 의미가 있다. 명말청초에 문인들이 아취(雅趣)의 삶을 추구했는데, 그의 식물의 상징성 및 식물 완상 방식은 그 당시 문인들의 아취의 삶을 반영할 수 있다고 생각한다.

• Molecules and Cells
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• 제25권3호
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• pp.368-375
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• 2008

Approximately 120 UDP-glycosyltransferases (UGTs), which are classified into 14 distinct groups (A to N), have been annotated in the Arabidopsis genome. UGTs catalyze the transfer of sugars to various acceptor molecules including flavonoids. Previously, UGT71C1 was shown to glycosylate the 3-OH of hydroxycinnamates and flavonoids in vitro. Such secondary metabolites are known to play important roles in plant growth and development. To help define the role of UGT71C1 in planta, we investigated its expression patterns, and isolated and characterized a loss-of-function mutation in the UGT71C1 gene (named ugt71c1-1). Our analyses by quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR), microarray data mining, and histochemical detection of GUS activity driven by the UGT71C1 promoter region, revealed the tissue-specific expression patterns of UGT71C1 with highest expression in roots. Interestingly, upon treatment with methyl viologen (MV, paraquat), ugt71c1-1 plants displayed enhanced resistance to oxidative stress, and ROS scavenging activity was higher than normal. Metabolite profiling revealed that the levels of two major glycosides of quercetin and kaempferol were reduced in ugt71c1-1 plants. In addition, when exposed to MV-induced oxidative stress, eight representative ROS response genes were expressed at lower levels in ugt71c1-1 plants, indicating that ugt71c1-1 probably has higher non-enzymatic antioxidant activity. Taken together, our results indicate that ugt71c1-1 has increased resistance to oxidative stress, suggesting that UGT71C1 plays a role in some glycosylation pathways affecting secondary metabolites such as flavonoids in response to oxidative stress.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2002년도 가을 학술발표회 논문집
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• pp.549-554
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• 2002

In Powder System, SCC demands high dosage of superplasticizer and a lage amout of powder for suitable fluidity and viscosity. Okamura's method of most representative mixing design method in SCC of Powder-System is unfavorable economically because of using a large amount of powder. In addition, many ready-mixed concrete plants do not use his mix design method and procedure due to complexity for practical application. Therefore, Nan Su proposed more simple mix design method than Okamura's. It had an advantage in simplicity in practical application and required a smaller amount of powders compared with Okamura's method. This paper proposed an optimal mixture proportion of SCC with consideration of Nan Su's method. The new and modified mix design method required a smaller amount of powder than that of Nan Su's. To check the properties of SCC, considered with the requirements specified by the Japanese Society of Civil Engineering.(JSCE)

• 한국잡초학회지
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• 제8권2호
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• pp.133-140
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• 1988

One hundred and one noxious weeds on cultivated land of Taiwan were investigated for the occurrence of "Kranz" leaf anatomy and activities of PEP carboxylase and $C_4$ acid decarboxylating enzymes : NADP-malic enzyme, NAD-malic enzyme, PEP carboxykinase. Based on the leaf anatomy and a/b chlorophyll ratio, twenty-seven species exhibit "Kranz" type leaf anatomy, and seventy-four species were found without it. Among the species investigated, Digitaria radicosa (Presl) Miq., Leptochloa chinensis (L.) Nees, and Sporobolus fertilis (Steud.) W. D. Clayton in the Gramineae were first recorded as $C_4$ plants. Twenty-sven species of "Kranz" type leaf anatomy, include those of monocotyledon ; sixteen species in Gramineae, six species in Cyperaceae. Those of dicotyledon ; two species each in Euphorbiaceae and Amaranthaceae and one species in Portulacaceae. The subtype of fourteen previously uninvestigated species among twenty-seven species were further determined. The properties of the three decarboxylating enzyme from representative species were also characterized.

• 한국가시화정보학회지
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• 제14권1호
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• pp.27-32
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• 2016

The reduction of $CO_2$ emissions has been a key target in the Marine Industry since the IMO's Marine Environment Protection Committee published its findings in 2009. The representative emission index is termed as the EEDI (Energy Efficiency Design Index) for the new ships. Among various flow control techniques ever proposed, the air lubrication method is the one of most promising one in terms of practical applicability. The present study examines the basic characteristics of the flat plate test with intention of applying the air lubrication technology to the reduction of the resistance of a ship. Image analysis technique is proposed as a tool to quantify the effectiveness of the air lubrication method.

• 한국안전학회지
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• 제32권3호
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• pp.21-27
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• 2017

Even if several chemical accident prevention systems such as PSM(Process Safety Management), RMP(Risk Management Plan), etc. have been carried out, many chemical accidents have still occurred at industrial plants in Korea. We describe the status of chemical industry and the trend of occurrence of chemical accidents in Korea. And this paper analyzes the recent chemical accidents in eight ways. These ways include chemical accident forms, ignition sources, sources of chemical equipment, human vs equipment/material causes, worker's working situation, employee scale, hazardous substances, week & time, fatalities of manufacture & contractor's workers. Finally we proposes the four representative prevention measures brought to result of cause analysis by accident statistics.