• Proceedings of the Korea Database Society Conference
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• 1994.09a
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• pp.71-103
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• 1994

중복데이타 서버(replication server) 는 자주 사용되는 데이타의 부분 또는 전부를 뷰 형태로 여러 지역에 중복하여 저장함으로써 최종 사용자가 원하는 데이터에 빨리 접근할 수 있도록 해준다. 또한 기본 테이블의 변화된 내용을 주기적으로 뷰에 반영함으로써 데이타 동시성의 문제를 완화하며 통신량을 감소시킬 수 있다. 본 연구에서는 기본 테이블에 일어난 변화를 저장뷰(materialized view)에 반영시켜주기 위해 테이블 전체를 읽는 방식을 피하고 일정기간 동안 테이블에 일어난 변화가 기록된 로그(log)를 이용하는 디프런셜 갱신(differential update) 방법을 사용한다. 이 방법은 테이블의 잠금(locking) 을 피함으로 시스템의 성능을 향상시 킬 수 있다. 또한 갱신에 관련된 통신량을 최소화하기 위한 기법들을 제안한다. 위의 방법을 이용하여 분산 상황에서 조인 저장뷰(join materialized view)의 갱신을 효과적으로 지원해 주는 중복데이타 서버(replication server)의 구조에 관해 연구한다.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.25 no.8A
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• pp.1096-1103
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• 2000

The default server strategy is commonly used for location and state managements of mobile host in mobile computing. With this strategy, we can find the cell of destination mobile host to send data by querying the default server. In SDN(single Default Notification) strategy which is a kind of default server strategy, the call is established after the location and state of the callee is acquired to the query server by querying the default server. But the communication cost overhead from the default server is increased if there are large number of cells and query requests, and if it is too far from the default server to a base station. Still more it will be unable to establish any calls to a mobile host when there is a fault in the default server of this host. In this paper, we suggest add evaluate a default server replication strategy to reduce the communication cost overhead and to make the service available.

• Journal of Korea Multimedia Society
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• v.7 no.5
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• pp.689-697
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• 2004

Rapid advances in mobile computing and the availability of wireless communications will soon provide mobile users with the ability to access data regardless of the location of the user or of the data. SRA(Static Replica Allocation) that is traditional scheme has been used for the replication method on the server. This replicates the data on the replica server after a moving host has been transferred to the cell. This strategy is simple and can easily relocate data. However, if a mobile user does not exist in the cell, the replicated data can be deleted in order to maintain data consistency. In addition to, if the mobile host leaves from replicated cell, it is difficult to access data in terms of replication route. Therefore, this paper proposes a new method of relocation based on data consistency strategy called USRAC(User Selection Replica Allocation based on Consistency) and also analyzes access cost according to the moving rate of mobile users, according to the access rate of mobile hosts, and according to the number of cells of mobile users and mobile hosts.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.10 no.10
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• pp.4681-4702
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• 2016

Recently, Massive energy consumption in Cloud Storage System has attracted great attention both in industry and research community. However, most of the solutions utilize single method to reduce the energy consumption only in one aspect. This paper proposed an energy effective gear-shifting mechanism (E2GSM) in Cloud Storage System to save energy consumption from multi-aspects. E2GSM is established on data classification mechanism and data replication management strategy. Data is classified according to its properties and then be placed into the corresponding zones through the data classification mechanism. Data replication management strategies determine the minimum replica number through a mathematical model and make decision on replica placement. Based on the above data classification mechanism and replica management strategies, the energy effective gear-shifting mechanism (E2GSM) can automatically gear-shifting among the nodes. Mathematical analytical model certificates our proposed E2GSM is energy effective. Simulation experiments based on Gridsim show that the proposed gear-shifting mechanism is cost effective. Compared to the other energy-saved mechanism, our E2GSM can save energy consumption substantially at the slight expense of performance loss while meeting the QoS of user.

• Journal of Veterinary Science
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• v.21 no.2
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• pp.33.1-33.15
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• 2020

Bovine ephemeral fever virus (BEFV) causes bovine ephemeral fever, which can produce considerable economic damage to the cattle industry. However, there is limited experimental evidence regarding the underlying mechanisms of BEFV. Annexin A2 (AnxA2) is a calcium and lipid-conjugated protein that binds phospholipids and the cytoskeleton in a Ca²⁺-dependent manner, and it participates in various cellular functions, including vesicular trafficking, organization of membrane domains, and virus proliferation. The role of the AnxA2 gene during virus infection has not yet been reported. In this study, we observed that AnxA2 gene expression was up-regulated in BHK-21 cells infected with the virus. Additionally, overexpression of the AnxA2 gene promoted the release of mature virus particles, whereas BEFV replication was remarkably inhibited after reducing AnxA2 gene expression by using the small interfering RNA (siRNA). For viral proteins, overexpression of the Matrix (M) gene promotes the release of mature virus particles. Moreover, the AnxA2 protein interaction with the M protein of BEFV was confirmed by GST pull-down and co-immunoprecipitation assays. Experimental results indicate that the C-terminal domain (268-334 aa) of AxnA2 contributes to this interaction. An additional mechanistic study showed that AnxA2 protein interacts with M protein and mediates the localization of the M protein at the plasma membrane. Furthermore, the absence of the AnxA2-V domain could attenuate the effect of AnxA2 on BEFV replication. These findings can contribute to elucidating the regulation of BEFV replication and may have implications for antiviral strategy development.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2001.10a
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• pp.233-236
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• 2001

This study explores on the static and dynamic growth patterns of high-tech ventures in Korea. We developed an integrative framework with target market (local vs. global), product/market maturity (existing vs. emerging), and technological capability (follower vs. pioneer). We also identified seven new ventures strategies as follows: i) reactive imitation, ii) proactive localization, iii) import substitution, iv) creative imitation, v) early market-entry, vi) global niche, and vii) global innovation. With five successful Korean new ventures, we found different competitive behaviors and performance among new venture strategic types. This study also observed two different growth patterns: growth through strategic replication and growth through strategic change. It depends on whether they are pursuing similar strategy over time or different strategy within for growth. In addition, we found that creative imitation plays a stepping-stone role in two-step internationalization processes. Although this study is exploratory and needs more empirical studies, it can provide new ventures with meaningful guidelines for growth and internationalization with a dynamic perspective.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.3-3
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• 2021

The ongoing global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has not only influenced over 1.26 billion people but also caused 2.77 million deaths worldwide (as of March 28, 2021). The vaccination could be the most efficient strategy to prevent SARS-CoV-2 infection. However, the continuous emergence of novel variants such as VUI-202012/01 (United Kingdom) and 501.V2 (South Africa) raises huge concerns about the effectiveness of the vaccine designed to target the original virus strain. Since ancient times regardless of the East and West, the plants which refered in this presentation have been consumed not only as food but also as a natural medicine to treat diverse diseases including infectious diseases. Importantly, these plants contain secondary metabolites that display antiviral activity involved in the inhibition of viral adsorption, penetration, and replication. Also, plant-derived natural medicines are expected to have a wider range of efficacy and fewer side effects than synthetic medicine, discovering novel plant-based viral agents would be a promising strategy to fight against SARS-CoV-2.

• BMB Reports
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• v.53 no.3
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• pp.166-171
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• 2020

A chemical library comprising 2,354 drug-like compounds was screened using a transcription and replication-competent viruslike particle (trVLP) system implementing the whole Ebola virus (EBOV) life cycle. Dose-dependent inhibition of Ebola trVLP replication was induced by 15 hit compounds, which primarily target different types of G protein-coupled receptors (GPCRs). Based on the chemical structure, the compounds were divided into three groups, diphenylmethane derivatives, promazine derivatives and chemicals with no conserved skeletons. The third group included sertindole, raloxifene, and ibutamoren showing prominent antiviral effects in cells. They downregulated the expression of viral proteins, including the VP40 matrix protein and the envelope glycoprotein. They also reduced the amount of EBOV-derived tetracistronic minigenome RNA incorporated into progeny trVLPs in the culture supernatant. Particularly, ibutamoren, which is a known agonist of growth hormone secretagogue receptor (GHSR), showed the most promising antiviral activity with a 50% effective concentration of 0.2 μM, a 50% cytotoxic concentration of 42.4 μM, and a selectivity index of 222.8. Here, we suggest a strategy for development of anti-EBOV therapeutics by adopting GHSR agonists as hit compounds.

• Biomolecules & Therapeutics
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• v.30 no.5
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• pp.427-434
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• 2022

The drug repurposing strategy has been applied to the development of emergency COVID-19 therapeutic medicines. Current drug repurposing approaches have been directed against RNA polymerases and viral proteases. Recently, we found that the inhibition of the interaction between the SARS-CoV-2 structural nucleocapsid (N) and spike (S) proteins decreased viral replication. In this study, drug repurposing candidates were screened by in silico molecular docking simulation with the SARS-CoV-2 structural N protein. In the ChEMBL database, 1994 FDA-approved drugs were selected for the in silico virtual screening against the N terminal domain (NTD) of the SARS-CoV-2 N protein. The tyrosine 109 residue in the NTD of the N protein was used as the center of the ligand binding grid for the docking simulation. In plaque forming assays performed with SARS-CoV-2 infected Vero E6 cells, atovaquone, abiraterone acetate, and digoxin exhibited a tendency to reduce the size of the viral plagues without affecting the plaque numbers. Abiraterone acetate significantly decreased the accumulation of viral particles in the cell culture supernatants in a concentration-dependent manner. In addition, abiraterone acetate significantly decreased the production of N protein and S protein in the SARS-CoV-2-infected Vero E6 cells. In conclusion, abiraterone acetate has therapeutic potential to inhibit the viral replication of SARS-CoV-2.

• Journal of Microbiology and Biotechnology
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• v.27 no.2
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• pp.380-387
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• 2017

The foamy viruses are currently considered essential for development as vectors for gene delivery. Previous studies demonstrated that prototype foamy virus (PFV) can infect and replicate prevalently in a variety of cell types for its exclusive replication strategy. However, the virus-host interaction, especially PFV-transportin3 (TNPO3), is still poorly understood. In our investigation of the role of TNPO3 in PFV infection, we found lower virus production in TNPO3 knockdown (KD) cells compared with wild-type 293T cells. PCR analysis revealed that viral DNAs were mostly altered to circular forms: both 1-long terminal repeat (1-LTR) and 2-LTR in TNPO3 KD cells. We therefore suggest that TNPO3 is required for successful PFV replication, at least at/after the nuclear entry step of viral DNA. These findings highlight the obscure mysteries of PFV-host interaction and the requirement of TNPO3 for productive infection of PFV in 293T cells.