• 한국정보통신학회논문지
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• 제24권8호
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• pp.1052-1057
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• 2020

ICT 신기술의 발달과 함께, 차량용 스마트키도 ICT 기술이 적용된 단말기이다. 따라서 차량과 스마트키가 RF통신을 할 때, 사이버 해킹공격이 가능하다. 스마트키에 대한 사이버 공격으로 차량 탈취 및 차량 통제권의 위협을 가져다줄 수 있다. 따라서 자율주행차량용 스마트키에 대한 해킹 공격 및 취약점에 대한 연구가 필요하다. 본 논문에서는 차량과 스마트키에 대한 RF 통신에 대한 사이버공격 사례를 분석한다. 그리고 스마트키에 대한 실제 RF 사이버공격을 시행하고, 동일 주파수대역의 전파 복제에 대한 취약점을 찾는다. 본 논문에서는 차량과 스마트키의 RF통신의 취약점을 분석하고, 사이버보안 대응방안을 제안한다. 향후 자율주행차량의 대중화로 사이버 공격 및 보안성 강화 방안은 인간 및 차량의 안전을 지킬 수 있는 기초자료가 될 것이다.

• 한국미생물·생명공학회지
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• 제50권1호
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• pp.63-70
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• 2022

Two small cryptic plasmids, pHG1 and pHG2, were isolated from Levilactobacillus zymae (formerly Lactobacillus zymae) GU240 and characterized. pHG1 is 1,814 bp in size with a GC content of 37.4% and contains two open reading frames. orf1 can potentially encode a protein of 101 amino acids (aa) with 99% identity with the copy number control protein of Lacticaseibacillus paracasei. orf2 can potentially encode a protein of 230 aa with 99% identity with a replication protein from multiple species. Six inverted repeats (IR I-VI) and six direct repeats (DR I-VI) were found in pHG1. pHG2 is 2,864 bp in size, with a GC content of 39.6%. pHG2 has two orfs. orf1 might encode a protein with 99% identity with the TrsL transmembrane protein. orf2 might encode a protein with 99% identity with plasmid recombination proteins from lactic acid bacteria. Both pHG1 and pHG2 may be useful as frames for constructing lactic acid bacteria-Escherichia coli shuttle vectors.

• Fisheries and Aquatic Sciences
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• 제25권6호
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• pp.320-326
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• 2022

Most of the emerging diseases that threaten humans are caused by RNA viruses which are extremely mutable during evolution. The fish RNA virus, viral hemorrhagic septicemia virus (VHSV) can infect a broad range of aquatic animal hosts, but the transmissibility of VHSV to mammals has not been thoroughly investigated. Therefore, our study aimed to investigate the potential adverse effects of VHSV in mammals. Briefly, the survival of VHSV was determined using only minimum essential media (MEM-2) and mammalian SNU-1411 and hepa-1c1c7s cells at 15℃ and 37℃. Mice (Mus musculus, 27.3 ± 1.9 g) were intravenously injected with VHSV (2.37E+05 TCID₅₀·mice^-1) in triplicate. Clinical signs and survival rates were examined at 14 days post-challenge, and infection was confirmed in the surviving mice. The 50% tissue culture infective dose (TCID₅₀) and polymerase chain reaction analysis were used to determine viral titers and the infection rate, respectively. The titer of VHSV suspended in MEM-2 at 15℃ was reduced by only one log after 8 days, whereas the virus maintained at 37℃ was inactivated 8 days post-inoculation (dpi). There were no recognizable cytopathic effects in either SNU-1411 or hepa-1c1c7s cells inoculated with VHSV at 15℃ and 37℃. VHSV in those cell lines at 37℃ was rapidly decreased and eventually inactivated at 12 dpi, whereas virus at 15℃ remained at low concentrations without replication. In vivo experiment showed that there were no signs of disease, mortality, or infection in VHSV-infected mice. The results of this study indicate that it is highly unlikely that VHSV can infect mammals including humans.

• 한국어병학회지
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• 제35권2호
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• pp.141-155
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• 2022

Rock bream iridovirus (RBIV) causes high mortality and economic losses in the rock bream (Oplegnathus fasciatus) aquaculture industry in Korea. Viral open reading frames (ORFs) expression profiling at different RBIV infection stages was investigated using microarray approaches. Rock bream were exposed to the virus and held for 7 days at 23 ℃ before the water temperature was reduced to 17 ℃. Herein, 28% mortality was observed from 24 to 35 days post infection (dpi), after which no mortality was observed until 70 dpi (end of the experiment). A total of 27 ORFs were significantly up- or down-regulated after RBIV infection. In RBIV-infected rock bream, four viral genes were expressed after 2 dpi. Most RBIV ORFs (26 genes, 96.2%) were significantly elevated between 7 and 20 dpi. Among them, 12 ORF (44.4%) transcripts reached their peak expression intensity at 15 dpi, and 14 ORFs (51.8%) were at peak expression intensity at 20 dpi. Expression levels began to decrease after 25 dpi, and 92.6% of ORFs (25 genes) were expressed below 1-fold at 70 dpi. From the microarray data, in addition to the viral infection, viral gene expression profiles were categorized into three infection stages, namely, early (2 dpi), middle (7 to 20 dpi), and recovery (25 and 70 dpi). RBIV ORFs 009R, 023R, 032L, 049L, and 056L were remarkably expressed during RBIV infection. Furthermore, six ORFs (001L, 013R, 052L, 053L, 058L, and 061L) were significantly expressed only at 20 dpi. To verify the cDNA microarray data, we performed quantitative real-time PCR, and the results were similar to that of the microarray. Our results provide novel observations on broader RBIV gene expression at different stages of infection and the development of control strategies against RBIV infection.

• Journal of Veterinary Science
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• 제21권3호
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• pp.50.1-50.16
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• 2020

Background: Porcine parvovirus (PPV) is a single-stranded DNA virus that causes porcine reproductive failure. It is of critical importance to study PPV pathogenesis for the prevention and control of the disease. NS1, a PPV non-structural protein, is participated in viral DNA replication, transcriptional regulation, and cytotoxicity. Our previous research showed that PPV can activate nuclear factor kappa B (NF-κB) signaling pathway and then up-regulate the expression of interleukin (IL)-6. Objectives: Herein, the purpose of this study is to determine whether the non-structural protein NS1 of PPV also has the same function. Methods: Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), enzyme-linked immunosorbent assay, western blot, immunofluorescence assay and small interfering RNA (siRNA) were used. Results: Our findings demonstrated that PPV NS1 protein can up-regulate the expression levels of IL-6 and tumor necrosis factor-alpha in a dose-dependent manner. Moreover, PPV NS1 protein was found to induce the phosphorylation of IκBα, then leading to the phosphorylation and nuclear translocation of NF-κB. In addition, the NS1 protein activated the upstream pathways of NF-κB. Meanwhile, TLR2-siRNA assay showed TLR2 plays an important role in the activation of NF-κB signaling pathway induced by PPV-NS1. Conclusions: These findings indicated that PPV NS1 protein induced the up-regulated of IL-6 expression through activating the TLR2 and NF-κB signaling pathways. In conclusion, these findings provide a new avenue to study the innate immune mechanism of PPV infection.

• 생물정신의학
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• 제29권2호
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• pp.33-39
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• 2022

Objectives The early growth response 3 (EGR3) gene located in chromosome 8p21.3 is one of the susceptibility loci in many psychiatric disorders. EGR3 gene plays critical roles in signal transduction in the brain, which is involved in neuronal plasticity, neuronal development, learning, memory, and circadian rhythms. Recent studies have suggested EGR3 as a potential susceptibility gene for bipolar disorder (BPD). However, this requires further replication with an independent sample set. Methods To investigate the genetic role of EGR3 in Korean patients, we genotyped six single-nucleotide polymorphisms (SNPs) in the chromosome region of EGR3 in 1076 Korean BPD patients and 773 healthy control subjects. Results Among the six examined SNPs of EGR3 (rs17088531, rs1996147, rs3750192, rs35201266, rs7009708, rs1008949), SNP rs35201266, rs7009708, rs1008949 showed a significant association with BPD (p = 0.0041 for rs35201266 and BPD2, p = 0.0074 for rs1008949 and BPD, p = 0.0052 for rs1008949 and BPD1), which withstand multiple testing correction. In addition, the 'G-C-C-C' and 'G-C-G-C' haplotypes of EGR3 were overrepresented in the patients with BPD (p = 0.0055, < 0.0001, respectively) and the 'G-T-G-C' haplotype of EGR3 was underrepresented in patients with BPD (p = 0.0040). Conclusions In summary, our study supports the association of EGR3 with BPD in Korean population sample, and EGR3 could be suggested as a compelling susceptibility gene in BPD.

• Animal Bioscience
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• 제36권11호
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• pp.1632-1646
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• 2023

Objective: The objective of the present study was to investigate the effect of dietary betaine (BT) supplementation on the hepatic transcriptome profiles in broiler chickens raised under heat stress (HS) conditions. Methods: A total of 180 (21-d-old) Ross 308 male broiler chicks were allotted to 1 of 3 treatment groups with 6 replicated cages in a completely randomized design. One group was kept under thermoneutral conditions at all times and was fed a basal diet (PC). Other 2 groups were exposed to a cyclic heat stress condition. One of the 2 groups under heat stress conditions was fed the basal diet as a negative control (NC), whereas the other group was fed the basal diet supplemented with 0.2% BT. All chickens were provided with diets and water ad libitum for 21 d. Following the experiment, the liver samples were collected for RNA sequencing analysis. Results: Broiler chickens in NC and BT group had decreased (p<0.05) growth performance. In the transcriptome analysis, the number of differentially expressed genes were identified in the liver by HS conditions and dietary BT supplementation. In the comparison between NC and PC treatments, genes related to energy and nucleic acid metabolism, amino acid metabolism, and immune system were altered by HS, which support the reason why heat-stressed poultry had decreased growth performance. In the comparison between NC and BT treatments, genes related to lipid metabolism, carbohydrate metabolism, and immune system were differently expressed under HS conditions. Conclusion: HS negatively impacts various physiological processes, including DNA replication, metabolism of amino acids, lipids, and carbohydrates, and cell cycle progression in broiler chickens. Dietary BT supplementation, however, offers potential counteractive effects by modulating liver function, facilitating gluconeogenesis, and enhancing immune systems. These findings provide a basis for understanding molecular responses by HS and the possible benefits of dietary BT supplementation in broiler chickens exposed to HS.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2002년도 가을 학술발표논문집
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• pp.133-135
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• 2002

DE와 GE의 급여가 어린 병아리의 골격발달에 미치는 영향을 알아보기 위해 1일령된 Hy-Line Brown 수평아리 4처리$\times$4반복$\times$5수/반복 100마리에게 3주간 대조군으로 IF 함량이 매우 낮은 SPC를 급여했다. 실험군은 DE와 GE를 1. 0, 2.0mM 두 수준으로 첨가 급여하였다. 이때 사료의 Ca 수준은 골격발달에 좀더 민감한 반응을 나타낼 수 있도록 요구량의 50% 수준으로 했으며, Phosphate는 정상수준으로 급여하였다. 식이섭취량과 체중증가량은 군간에 차이를 보이지 않았고, ALP activity는 GE군과 대조군에 비해 DE군에서 유의하게 감소하였으나(p〈0.05) 첨가수준간에는 차이가 없었다. 경골의 회분 %는 대조군에 비해 DE, GE군에서 유의하게 증가하였으나(P〈0.05), 두 IF간 및 첨가수준 간에는 차이가 없었다. 경골의 파괴강도는 모든 처리군에서 유의차를 보이지 않았다. 즉 DE의 급여가 GE에 비해 ALP를 유의적으로 감소시켰지만, 경골의 회분 %와 경골 파괴강도에 있어서는 DE와 GE군 간에 급여수준에 따른 차이를 보이지 않아 이에 대한 연구가 더 필요할 것으로 사료된다.

• 한국가금학회지
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• 제28권3호
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• pp.275-281
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• 2001

본 시험은 키토산 및 녹차의 급여가 산란계의 사양성적 및 혈액과 난황의 콜레스테롤 수준에 미치는 영향을 관찰하기 위하여 실시하였다. 시험은 옥수수-밀기울 위주 사료로 처리당 3반복 반복당 30수씩 60주령 갈색 산란계를 총 360수 공시하여 8주간에 걸쳐 키토산을 0.15%를 첨가(T1), 녹차잎을 0.15% 첨가 (T2) 및 키토산과 녹차잎을 각각 0.15% 첨가 (T3)하여 수행하였다. 사료섭취량은 대조구가 약 830g으로 가장 낮았고, 처리구에서는 T2구가 낮은 경향을 보였으나, 산란율은 대조구의 52.6%에 비하여 T1구가 55.7%로 높은 경향을 보였으며, T2(59.3%) 및 T3구 (58.4%)는 유의적 (P<0.05)으로 높았다. 난중은 대조구가 63.8g으로 T1구의 62.3g에 비하여 높은 경향이었으나, T2및 T3구의 65g에 비하여 낮은 경향을 나타냈다. 혈중 콜레스테롤 함량은 시험 개시 시에 비하여 8주후에 처리구 (T1, T2 및 T3)에서 114∼123mg/dl로 유의적으로 감소하였으며 (P<0.05), 처리간 혈중 콜레스테롤 함량은 T2가 다른 처리구에 비하여 낮은 경향을 보였다. 그러나 난황 콜레스테롤 농도에는 키토산과 녹차의 첨가가 영향을 미치지 못하였다.

• 한국가금학회지
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• 제32권3호
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• pp.219-224
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• 2005

본 연구는 산란계에서 장석의 첨가가 난 생산과 난 성분에 미치는 영향을 구명하기 위하여 실시하였다. 공시동물은 갈색테트라 산란계 96수를 4처리 4반복으로 반복당 6수씩 3단 철제 cage에서 사양시험을 실시하였다. 시험사료는 대조구를 기초사료로 이용하였으며, 장석을 수준별로 $0.5\%,\;1.0\%$ 및 $1.5\%$로 첨가하여 배합하였다. 산란율은 대조구와 비교하여 장석 $0.5\%$와 $1.0\%$ 첨가구에서 증가하였으며, 통계적인 유의차를 보였다(P<0.05). 장석 0.5, 1.0및 $1.5\%$첨가구에서 산란계의 난중, 산란량, 사료섭취량, 사료요구율, 난각강도 및 난황색은 대조구에 비해 유의차가 없었다(P>0.05). 계란의 하우 유니트와 혈반은 장석 $1.5\%$ 첨가구에서 유의적으로 증가하였으며(P<0.05), 난백 높이와 잔반은 유의차를 보이지 않았다(P>0.05). 관능검사 결과 다즙성은 대조구가 2.78로 가장 낮았고, 장석 $1.0\%$ 첨가구가 3.57로 가장 높아 통계적 유의차를 보였다(P<0.05).종합적인 기호도에서 장석 $1.0\%$ 첨가구가 3.70으로 가장 높은 기호도를 보였다.