• 대한간호학회지
• /
• 제26권2호
• /
• pp.359-371
• /
• 1996

This study has been done for the purpose of testing the effect of health education on the performance of health promoting behavior in college students, and identifying the factors affecting health promoting behaviors. A Nonequivalent control group posttest research design was used. Two hundred thirty college students at K College in T city were studied. Of them, 114 who attended a systematic health education session for three hours a week during one semester were the experimental group. And 116 college students who were chosen of matched sampling of grade, class and sex were the control group. This study was conducted from March 1 to July 2, 1995. The instruments used for this study included a survey of general characteristics, perceived health status, self-esteem, health promoting behavior and health locus of control. Analysis of data was done by use of mean, 1-test, Pearson correlation coefficient and multiple regression. The results of this study are summarized as follows : 1) The average item score for the health promoting behavior was low at 2.52. In the sub-categories, the highest degree of performance was ‘harmonious relationships’, following ‘sanitary life’, ‘self-esteem’, ‘rest and sleep’, and ‘emotional support’ and the lowest degree was ‘professional health management’. 2) Hypothesis 1 that the college students who get health education will have a higher degree of health promoting behavior than the college students who do not get health education was accepted. There was a statistically significant difference between the average of the experimentalgroup, 2.60, and the average of the control group, 2.45.（t＝11.30, p＝0.0009）. 3) Hypothesis 2 that the college students who get health education will have a higher score of perceived health status than college students who do not get the health education was rejected. （t＝1.13, p＝0.289） 4) Performance of health promoting behavior was positively correlated with self-esteem and grade and negatively correlated with perceived health status. 5) The most important factor affecting performance of health promoting behavior was self-esteem. The following suggestions are made based on the above results : 1) Replication of the research is needed to confirm effects of health education. 2) More effective health education programs need to be developed through by modification of teaching methods and content analysis of health education. 3) Other factors affecting health promoting behavior should be identified. 4) Nursing colleges or departments of nursing should make an effort to develop and carry out various health education programs for the health promotion of all college students.

• 대한간호
• /
• 제37권4호
• /
• pp.72-84
• /
• 1998

This study has been done for the purpose of testing the effects of education for women's health on the performance of health promoting behavior and self esteem of college women. The study was designed as simulated control group pretest-posttest design, the experimental group was composed 182 college women and the control group was composed 151 college women at D University in Seoul. The instruments used for this study were Health Promoting Behavior Scale developed by Walker et al(1987)were modified by Shin(1997) and Self Esteem Scale developed by Rosenberg(1965). The data were analyzed by chi-square test, t-test and ANCOVA using $SPSSIPC^+$ program. The results were as follows: 1. The result of the homogeneity test in terms of the demographic characteristics of two group showed that there were significant difference in major discipline($x^2=155.854$, p=.000), religion($x^2=6.325$, p=.011), and disease experience($x^2=3.949$, p=.046). 2. Hypotheses 1 that the college women who get women's health education will have a higher score of health promoting behavior than the college women who do not get women's health education was supported. The score of health promoting behavior between two group showed statistically difference(t=7.25, p= .000). Mer control of covariates(major discipline, religion, and disease experience), the score of health promoting behavior between two group showed statistically difference(F=31.817, p= .000). 3. Hypotheses 2 that the college women who get women's health education will have a higher score of self esteem than the college women who do not get women's health education was supported. The score of self esteem between two group showed statistically difference(t=4.91, p= .000). After control of covariates(major discipline, religion, disease experience), the score of self esteem between two group showed statistically difference(F=12.688, p= .000). The following suggestions are made based on the above results : 1) Replication of the research is needed to confirm effects of health education including the college man and various demographic differences. 2) More effective health education programs appropriate to subject need to be developed. 3) Nursing college or departments of nursing should make an effort to develop and carry out various health education program for health for all.

• Asian-Australasian Journal of Animal Sciences
• /
• 제21권9호
• /
• pp.1339-1347
• /
• 2008

The objective of this study was to determine the effects of green tea probiotics on growth performance, meat quality and immune response in finishing pigs, and to assess the possibility of substituting green tea probiotics for antibiotics in diets of finishing pigs. This green tea probiotics is made by mixing green tea powder and excipients (defatted rice bran and wheat bran) and fermenting the mixture with beneficial bacteria. A total of 90 crossbreed "Landrace$\times$Yorkshire" finishing pigs with an average body weight of $72.5{\pm}2.5kg$ were assigned to 5 dietary treatments in a completely randomized design. Each treatment had 3 replications with 6 pigs per replication. The five dietary treatments were control, antibiotic (0.003% chlortetracycline added) and 0.1, 0.5 and 1.0% of green tea probiotics. There were no significant differences in final body weight, daily weight gain, daily feed intake and feed conversion ratio in the green tea probiotics and antibiotic treatments (p>0.05). Crude protein content was significantly increased in the 0.1 and 1.0% green tea probiotics treatment groups (p<0.05) and there was no significant difference in crude fat content of the meat among the treatments. The TBA value of meat was significantly lowered with 0.5 and 1.0% green tea probiotics treatments compared to that of controls and statistically similar to the antibiotic treatment after 3 weeks of storage (p<0.05). The growth of spleen cells stimulated with Con A (0.1 and $1.0{\mu}g/ml$) was significantly increased with 1.0% green tea probiotics treatment compared to that of the control treatment (p<0.05). The growth of spleen cells stimulated with LPS (1.0, 3.0 and $10{\mu}g/ml$) was significantly increased in the 0.5% green tea probiotics group compared to the antibiotic group (p<0.05). In Con A ($1.0{\mu}g/ml$) medium, IL-6 production of spleen cells was significantly increased with 1.0% green tea probiotics treatment compared to that of the control (p<0.05). In LPS ($10.0{\mu}g/ml$) medium, TNF-${\alpha}$ production of spleen cells increased significantly in all green tea probiotics treatment groups compared to that of the control (p<0.05). Finally it can be summarized that addition of green tea probiotic has a positive effect similar to antibiotic and 0.5% is the suitable dietary supplementation dose for finishing pig production.

• 유기물자원화
• /
• 제18권4호
• /
• pp.77-85
• /
• 2010

양돈분뇨의 처리는 환경문제 해결과 자원으로서의 이용 등 중요한 의미가 있다. 본 연구에서는 양돈분뇨의 처리 및 자원화 방법으로 개발된 SCB 액비를 토양에 시용했을 때 양분이 작물에 흡수 이용되는 양과 토양중에 잔류하는 양을 조사하여 SCB 시용량 및 시용방법 결정을 위한 기초자료를 제공하고자 하였다. 무비, 화학비료, 저장액비, SCB 액비를 완전임의배치 5반복으로 처리하여 시험을 수행하였다. 벼의 생육은 화학비료구에서 가장 좋았고, 다음으로 저장액비, SCB 액비, 무비구 순이었다. 볏짚 수량 역시 화학비료 처리구에서 가장 많았으며, 다음으로 저장액비, SCB 액비, 무비구 순이었다. 생육상황은 수량까지 이어져 무비구는 처리구보다 감수 경향을 보였으며, 다른 처리구 간에는 대동소이하였다. 식물체의 무기성분 흡수량에서도 화학비료를 비롯한 처리구의 질소, 인산 등 각종 무기성분 흡수량이 무비구에 비하여 많았으나, 이를 제외한 처리 간에는 큰 차이가 없었다.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제1권2호
• /
• pp.149-153
• /
• 2000

We have constructed a recombinant baculovirus, Autographa californica nuclear polyhedrosis virus (AcNPV), containing green fluorescent protein (GFP) gene from the jellyfish, Aequorea victoria, and transferred it into the domestic silkworm Bombyx mori larvae for the production of visible transgenic silkworm of living organism. When one day-old fifth instar female larvae were injected with the recombinant AcNPV of 1x10$^{5}$ plaque forming units, the bright glow of GFP was detected in the recombinant AcNPV-infected larvae and in the newly hatched larvae of the next generation. Our findings demonstrate that the viral replication was detected in the silkworm treated with the recombinant ACNPV and the gfp gene was expressed under the transcriptional control of the polyhedrin gene promoter, Furthermore, the gfp gene was transmitted to the next generation, suggesting that this system can be applied for the development of transgenic silkworms.

• Journal of Acupuncture Research
• /
• 제18권4호
• /
• pp.101-115
• /
• 2001

Objective : To characterize the antitumorigenic potential of Apamin, one of the major components of bee venom, its effects on cell proliferation and the mitogen-activated protein kinase (MAPK) signal transduction pathway were characterized using the human melanoma cell line SK-MEL-2. Methods & Results : Cell counting analysis for cell death demonstrated that consistent with a previous results, SK-MEL-2 cells treated with $0.5-2.0{\mu}g/ml$ of Apamin showed no recognizable cytotoxic effect whereas detectable induction of cell death was identified at concentrations over $5.0{\mu}g/ml$. [3H]thymidine incorporation assay for cell proliferation demonstrated that DNA replication of SK-MEL-2 cells is inhibited by Apamin in a dose- and time-dependent manner. To explore whether Apamin-induced growth suppression is associated with the MAPK signaling pathway, phosphorylation of Erk, a function mediator of MAPK growth-stimulating signal, was examined Western blot assay using a phospho-specific Erkl/2 antibody. A significant increase of Erkl/2 phosphorylation level was observed in Apamin-treated cells compared with untreated control cells. Qantitative RT-PCR analysis revealed that Apamin inhibit expression of MAPK downstream genes such as c-Jun, c-Fos, and cyclin D1 but not expression of MAPK pathway component genes including Ha-Ras, c-Raf-1, MEK1, and Erk. Conclusion : It is strongly suggested that the antitumorigenic activity of Apamin might result in part from its inhibitory effect on the MAPK signaling pathway in human melanoma cells SK-MEL-2.

• IMMUNE NETWORK
• /
• 제15권2호
• /
• pp.91-99
• /
• 2015

Herpes simplex virus (HSV) is a common causative agent of genital ulceration and can lead to subsequent neurological disease in some cases. Here, using a genital infection model, we tested the efficacy of vinegar-processed flos of Daphne genkwa (vp-genkwa) to modulate vaginal inflammation caused by HSV-1 infection. Our data revealed that treatment with optimal doses of vp-genkwa after, but not before, HSV-1 infection provided enhanced resistance against HSV-1 infection, as corroborated by reduced mortality and clinical signs. Consistent with these results, treatment with vp-genkwa after HSV-1 infection reduced viral replication in the vaginal tract. Furthermore, somewhat intriguingly, treatment of vp-genkwa after HSV-1 infection increased the frequency and absolute number of $CD3^-NK1.1^+NKp46^+$ natural killer (NK) cells producing interferon (IFN)-${\gamma}$ and granyzme B, which indicates that vp-genkwa treatment induces the activation of NK cells. Supportively, secreted IFN-${\gamma}$ was detected at an increased level in vaginal lavages of mice treated with vp-genkwa after HSV-1 infection. These results indicate that enhanced resistance to HSV-1 infection by treatment with vp-genkwa is associated with NK cell activation. Therefore, our data provide a valuable insight into the use of vp-genkwa to control clinical severity in HSV infection through NK cell activation.

• 한국토양비료학회지
• /
• 제46권5호
• /
• pp.379-385
• /
• 2013

Although sorghum residue production was estimated to be $8{\sim}10Mg\;ha^{-1}$, most sorghum straw was used to be burnt or removed after harvest. This experiment was conducted to evaluate the effect of the incorporation of manured sorghum residues on soil physical properties from 2010 to 2012 in the converted paddy field. Five treatment with 3 replication consisted of control, inorganic fertilizer (NPK), manured residues, sorghum stover and sawdust manure. The incorporation level of organic source was $10Mg\;ha^{-1}$ without inorganic fertilizer NPK. The investigated physical parameters were bulk density (BD), porosity, water stability aggregate (WSA), water infiltration rater (WIR), penetration resistance (PR) and soil water retention characteristics (WRC) with soil depth. The incorporation of manured sorghum residues improved WIR significantly over inorganic fertilizer (NPK), sorghum residues and sawdust manure. The BD by incorporating with manured residues on sorghum was reduced significantly with crop residue over plot of NPK and sawdust. Significant increase in WSA was observed with stubble incorporation alone or manured sorghum residues. Soil WRC were significantly enhanced with manured sorghum residue incorporated without NPK. The average PR at 0~15 cm was 0.54 MPa for manured sorghum residues. For sawdust manure plot it was 0.42 MPa, for Sawdust manure plot 0.39 MPa and for NPK plot 0.54 MPa.

• 대한의생명과학회지
• /
• 제24권2호
• /
• pp.94-101
• /
• 2018

Tuberculosis (TB) is infectious disease caused by Mycobacterium tuberculosis (MTB) infection. It is known that not only the property of microorganism but also the genetic susceptibility of infected patients is controlled. Interleukin 2 (IL-2) is a cytokine belonging to type 1 T helper (Th1) activity. In addition, IL-2, when infected with MTB, binds IL-2 receptor and promotes T cell replication and is involved in granuloma formation. The aim of this study was to investigate the genetic polymorphisms of the IL-2 receptor gene in tuberculosis patients and normal individuals. We analyzed 22 SNPs in three genes using the genotype data of 443 tuberculosis cases and 3,228 healthy controls from the Korea Association Resource for their correlation with tuberculosis case. IL2RA, IL2RB, and IL2RG genes were genotyped of 16, 4, and 2 SNPs, respectively. Among three genes, only IL2RA gene polymorphisms showed statistically significant association with tuberculosis case. 6 SNPs with high significance were identified in the IL2RA gene. In addition, the linkage disequilibrium (LD) structure of IL2RA gene was confirmed. SNP imputation of IL2RA gene was performed, it was confirmed that more SNPs were significant between case and control. If we look at the results of IL2RA gene analysis above, we can see that genetic polymorphism in the gene expressing $IL-2R{\alpha}$ will regulate the expression level of $IL-2R{\alpha}$, and the change in the immune system involved in $IL-2R{\alpha}$. In this study, genetic polymorphism that may affect host immunity suggests that susceptibility to tuberculosis may be controlled.

• 대한의생명과학회지
• /
• 제23권2호
• /
• pp.133-137
• /
• 2017

Periodontitis is the major causation of tooth loss in the elderly population. Multiple risk factors include oral microorganisms, smoking, metabolic syndrome, and genetic factors influence periodontitis development. In this study, we conducted a replication study of using previous Korean GWAS results by examining an independent population. The study population was recruited from Mir Dental Clinic, Daegu, Korea. In total, 93 samples were evaluated from July 2016 to January 2017. The sample groups include relatively older patients (>60 years) with no periodontitis (n = 31) and relatively younger patients (range 40~60 years) with severe periodontitis (n = 62). A total of seven markers which were previously reported to be associated with periodontitis were genotyped. Among the seven SNPs, rs16846206 and rs2392510 showed a significant association by logistic regression analysis and Chi square test, respectively. The former SNP showed significant association with severe periodontitis, whereas this study also showed same tendency in which individuals with the minor allele are significantly more frequent in cases than those in controls. The SNP is located on a coding gene (SLC9C2), where the alanine residue 505 is replaced by glycine (Ala505Gly). The later SNP was significant when differed between case and control groups, but there was no significance by logistic regression analysis when controlled for age and sex as covariant. Although the study population size examined in the current study was relatively smaller compared to previous studies, our results implicated that at least the two SNPs (rs16846206 and rs2392510) might be important candidates for the further genetic study.