• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.23 no.5
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• pp.438-444
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• 2013

This paper presents the replication of a desired vibration response by iterative learning control (ILC) system for a vibration motion replication actuator. The vibration motion replication actuator has parameter uncertainties including system nonlinearity and joint nonlinearity. Vehicle manufacturers worldwide are increasingly relying on road simulation facilities that put simulated loads and stresses on vehicles and subassemblies in order to reduce development time. Road simulation algorithm is the key point of developing road simulation system. With the rapid progress of digital signal processing technology, more complex control algorithms including iterative learning control can be utilized. In this paper, ILC algorithm was utilized to produce simultaneously the six channels of desired responses using the Stewart platform composed of six linear electro-magnetic actuators with Halbach magnet array. The convergence rate and accuracy showed reasonable results to meet the requirement. It shows that the algorithm is acceptable to replicate multi-channel vibration responses.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.27 no.3
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• pp.450-454
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• 2003

It is important to control the processing conditions to maximize the replication quality of UV-molded microstructure. In the present study, the tip radius anil surface roughness of V-groove structure were measured to quantify the replication quality. UV-curing dose and the applied pressure were experimentally selected as the governing Processing conditions that affect the replication quality of the UV-molded part. Finally. an experimental optimization technique combining central composite design and desirability function approach was used to maximize the replication quality of UV-molded structure.

• Journal of Biosystems Engineering
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• v.26 no.4
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• pp.391-398
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• 2001

A robot system for clone replication and gridding, which is a preliminary state of the genome research, was developed and evaluated its performance. This gridding robot system consisted of 1) a gridding heat that replicated the clone, 2) a manipulator, as a part of body of robot, which transferred the gridding head along x-, y-, z-axis, 3) a well plate arranging board, 4) a sterilization unit, and 5) a control unit. Performance of the system was evaluated with 1) repeatability of the robot system, 2) clone replication efficiency, 3) time requirement of the replication, and 4) sterilization efficiency. The repeatability error of the robot system showed 0.219 mm and 0.094 mm in the direction of x- and y-axis, respectively. The success rate of the clone replication with the gridding head was 100% on the membrane filter. The time required for the replication was four minutes and fifty-five seconds from the four 96 well plates to a 384 well plate meanwhile the required time with well experienced hand labor was three minutes thirty-five seconds. The gridding operation of clone could not be done by hand labor and the required time with robot system for the gridding on the membrance filter with the control program 5$\times$5: 1 copy and 384 gridding pins was twenty minutes and twenty-five seconds. The efficiency of the sterilization was considered to be satisfactory since no growth of fungi was found around the area of replication in the membrane filter.

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.1078-1082
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• 2003

A feasibility study for the fabrication of master replication with nanostructures by Nanoimprint Lithography (NIL) was investigated for application of polymer Photonic Bandgap (PBG) devices used in photonic IC. Large area gratings of $9{\times}15(mm^2)$ with p = 400 nm was successfully embossed on PMMA on silicon wafer and the embossing parameters (temperature, pressure, time) were established. A precise control of $O_2$ plasma Reactive Ion Etching (RIE) process time allowed window opening over the whole area despite the presence of wafer bending. Master replication with aspect ratio 1 was successfully fabricated, but master replication with aspect ratio 3 needs to optimize parameters. All replications were done in a NIL process.

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.1332-1336
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• 2003

An experimental method is presented to maximize the replication quality of UV-molded micro-optical components. It is important to maximize the replication quality, because one can obtain the replicated micro-optical components with desired properties by accurate control of the shape. In the present study, a simple technique to avoid micro-air bubbles was first suggested. The effects of the UV-curing dose and the compression pressure on the replication quality of UV-molded structure were examined experimentally. Finally, as a practical application of the process design method, microlens arrays with diameters between 8 ${\mu}m$ and 96 ${\mu}m$ were fabricated by the present method, and the replication quality and the optical properties of the replicated microlens were measured and analyzed.

• Journal of KIISE:Information Networking
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• v.30 no.4
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• pp.545-558
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• 2003

It is expected that per-user customized services are widely used in next generation Personal Communication Network. The ultimate goal for personalized service is the Virtual home Environment (VHE) providing ´same-look-and-feel´ services for the subscriber wherever he roams to. To provide personalized services for each call, per-user service profiles are frequently referenced, so efficient service profile management is essentially required. To realized the VHE, typically two schemes, can be employed; One is Intelligent Network based service control and the other is a full replication scheme that always replicates profile in user´s current zone. The first scheme is referred as Central scheme and th second scheme is the modified replication scheme of IMT-2000, we refer to as Follow-Me Replication Unconditional (FMRU). Since the Central scheme only depends on the service cal rate and the FMRU is merely dependent on the movement rate, it is apparent that FMRU scheme outperforms the Central scheme if the call to mobility ratio (CMR) is large, and vice versa. In this paper, we propose a new service profile replication schemes, Adaptive Follow-Me Replication (AFMR) that determine replication automatically according to the user´s CMR. We compared the performance of the AFMR with the non-adaptive Follow-Me Replication unconditional on Demand (FMRUD) scheme. Performance results indicate that as the CMR of a user changes AFMR adapts well compared to the existing schemes.

• Journal of Microbiology
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• v.33 no.3
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• pp.191-195
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• 1995

IciA protein has been shown as an inhibitor for the initiation of E. coli chromosomal DNA replication at oriC. IciA protein binds the AT-rich region in oriC and then blocks the initiation of chromosomal DNA replication. Two binding sites for IciA protein were identified in dnaA gene, encoding the initiator for the E. coli chromosomal replication, promoter region by gel-shift assay and DNase I footprinting, One, named as IciA site I, is located upstream of the dnaA promoter 1P. The other, named as IciA site II, is located downstream of the dnaA promoter 2P. The sequence comparison of the regions protected from the DNase I cleavage did not result in a clear consensus sequence for the binding of IciA protein, suggesting that IciA protein may be a member of multimeric complex dsDNA binding proteins. This study provided information about the binding mode of IciA protein. Even though the IciA site II and IciA binding site in oriC seem to be composed of two IciA binding units, one binding unit is likely enough to cause the binding of IciA protein to the IciA site I. The binding of IciA protein to the dna4 promoter implies that IciA protein may involve not only the control of the initiation of chromosomal DNA replication but also the control of the dna4 gene expression.

• Korean Journal of Microbiology
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• v.32 no.2
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• pp.115-119
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• 1994

Replication control region of pSBK203, a chloramphenicol acetyltransferase conferring plasmid from Staphylococus aureus was cloned and its nucleotide sequence has been determined. Base sequence homology of this copy control region with those of plasmids belonging to pT181 family was obtained and analyzed. Copy number of four copy mutants derived by addtion or deletion of nucleotides in unique XbaI recognition site in copy control region of pSBK203 was also determined.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2000.04a
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• pp.167-170
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• 2000

Recently the sub-micron structured substrates of 0.74 ${mu}ell$ track pitch and 800 $\AA$groove depth are required for DVD-RAM and the track pitch is expected to be narrower as the need for the information storage density is getting higher. For the accurate replication of the land-groove structure in the stamper to the plastic substrates it is important to control the injection -compression molding process such that the integrity of the replication for the land-groove structure is maximized. in the present study polycarbonate substrates were fabricated by injection comression molding and the land-groove structure regarded as one of mold temperature and the compression pressure on the integrity of the replication were examined experimentally. An efficient design methodology using the response surface method (RSM) the central composite design(CCD) technique and the analysis-of-variance (ANOVA) was developed to obtain the optimum processing conditions which maximize the integrity of the replication with a limited number of experiments.

• Journal of Microbiology and Biotechnology
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• v.23 no.6
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• pp.837-842
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• 2013

A cryptic plasmid, pMBLR00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC 3733 was isolated, characterized, and used for the construction of a cloning vector to engineer Leuconostoc species. pMBLR00 is a rolling circle replication plasmid, containing 3,370 base pairs. Sequence analysis revealed that pMBLR00 has 3 open reading frames: Cop (copy number control protein), Rep (replication protein), and Mob (mobilization protein). pMBLR00 replicates by rolling circle replication, which was confirmed by the presence of a conserved double-stranded origin and single-stranded DNA intermediates. An Escherichia coli-Leuconostoc shuttle vector, pMBLR02, was constructed and was able to replicate in Leuconostoc citreum 95. pMBLR02 could be a useful genetic tool for metabolic engineering and the genetic study of Leuconostoc species.