• Title/Summary/Keyword: replica method

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Microcontact Printing of Bacteria Using Hybrid Agarose Gel Stamp (혼성 아가로즈젤 스탬프를 이용한 박테리아 마이크로 컨택트 프린팅)

  • Shim, Hyun-Woo;Lee, Ji-Hye;Lee, Chang-Soo
    • KSBB Journal
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    • v.21 no.4
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    • pp.273-278
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    • 2006
  • The noble method of hybrid agarose gel microstamp fabricated by replica molding against PDMS master to make bacteria patterns on agar surface was presented. After the fabricated hybrid agarose gel microstamp was inked with E. coli, we could obtain 2 dimensional bacterial arrays with $50{\mu}m$ circular spots. And the various shaped patterns based on experimental design were easily generated. The analysis of mean fluorescent signal was showed that bacterial pattern have high contrast between spots and background and homogeneity of pattern. Our proposed method solved the problem of wetting and handling with small soft agarose gel microstamp when bacteria were used for ink. The agarose gel stamp provides appropriate environment to inked bacteria, which is essential technology for cell patterning with high retaining viability during the patterning process. This method is reproducible, convenient, rapid, and could be applied to screening system, study of cell-surface interaction, and microbial ecology.

A Study on Making a Replica for Restoration of the Stone Lion Statue Excavated from Woljeong Bridge (월정교 출토 사자상 복원을 위한 모본 제작 연구)

  • Lee, Chan Young;Han, Kyeong Soon
    • Journal of Conservation Science
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    • v.31 no.2
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    • pp.147-157
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    • 2015
  • TExhibiting replicas of major artifacts is becoming common recently to raise their value and help the public to understand them. In Korea, more attention is paid only to appearance in creating replicas of artifacts. It is required to study reproduction technology minimizing damages to originals and applicable to massive artifacts like sculptures in the situation that replicas and artifact reproduction is increasing. To make a replica of the Stone Lion Statue excavated from Woljeong Bridge, a measured drawing and a cross section are produced based on art historical surveys using 3D scans. A non-contact reproduction method is selected. The strength of the non-contact reproduction method is that the output is detailed. Athough the non-contact method has known to be applicable only to small scale artifacts and require reprocess, it is discovered to be applicable to detailed statues like this lion statue. Therefore, this study may contribute to complement the historic authenticity of the statue as well as to raise its academic value. It also can be utilized as research material of similar artifacts'replicas and restoration.

Floating Primary Copy Method for Replica Control of Spatial Data (공간 데이터베이스의 중복 제어를 위한 Floating Primary Copy 기법)

  • 최진오
    • Proceedings of the Korea Multimedia Society Conference
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    • 2002.05c
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    • pp.68-72
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    • 2002
  • CIS 응용에서 공간 객체들이 여러 사이트에 걸쳐 중복 저장되어 있다면, 이 객체들의 수정 시 일관성 제어를 위한 중복 제어 기법이 필요하다. 그러나, 일반적인 비즈니스 데이터를 위한 정통적인 중복 제어기법은 공간 데이터에는 그대로 사용될 수 없다. 공간 데이터의 수정은 긴 트랜 션을 필요로 하며, 또한 일반 데이터와 달리 데이터간의 공간 종속성을 지니기 때문이다. 본 논문은 기존의 Primary Copy 중복 제어 기법을 확장하며, time-stamp에 기반하여 Primary Copy 사이트가 변경되는 Floating Primary Copy 기법을 제안한다. 본 논문에서 제안하는 중복 제어 기법은 Primary Copy 사이트를 동적으로 변경함으로써, 긴 트랜 션의 중복 일관성 제어를 지원할 수 있다.

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Degradation Estimation Of Material by Barkhausen Noise Analysis (바크하우젠 노이즈 해석에 의한 재료의 열화도 평가)

  • Lee Myung Ho
    • Transactions of the Korean Society of Machine Tool Engineers
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    • v.14 no.3
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    • pp.38-46
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    • 2005
  • The destructive method is reliable and widely used for the estimation of material degradation but it have time-consuming and a great difficulty in preparing specimens from in-service industrial facilities. Therefore, the estimation of degraded structural materials used at high temperature by nondestructive evaluation such as electric resistance method, replica method, Barkhausen noise method, electro-chemical method and ultrasonic method are strongly desired. In this study, various nondestructive evaluation(NDE) parameters of the Barkhausen noise method, such as MPA(Maximum Peak Amplitude), RMS, IABNS(Internal Area of Barkhausen Noise on Signal) and average amplitude of frequency spectrum are investigated and correlated with thermal damage level of 2.25cr-1.0Mo steel using wavelet analysis. Those parameters tend to increase while thermal degradation proceeds. It also turns out that the wavelet technique can help to reduce experimental false call in data analysis.

Effect of heat treatment of core fabricated by Ni-Cr alloy on marginal and internal fit (열처리가 Ni-Cr 합금으로 제작된 하부구조물의 변연 및 내면 간격에 미치는 영향)

  • Kim, Jae-Hong;Kim, Ki-Baek;Jung, Jae-Kwan
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.9
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    • pp.244-250
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    • 2017
  • The most important aspect determining the completeness of aprosthesis is itsmarginal and internal fit. Alloysare processed using a softening/hardening heat treatment methodin order to improve their mechanical, physical properties and polishing properties. To examinehow the heat treatment method affects the marginal and internal fit of the Ni-Cr alloy core,thirty dental stone models of the abutment of the mandibular left molar were manufactured.The Ni-Cr alloy coreswere manufactured by the dipping method for the experiment and dividedinto three groups; A for no heat treatment, B for softening heat treatment and C for hardening heat treatment. The marginal and internal fitsof all of the groups were measured by the silicone replica technique. A statistical analysis was performed using one-way ANOVA(${\alpha}=0.05$) in order to examine whether there is a significant difference in the average values of the marginal and internal fits among the three groups and it was found that themarginal fits (1, 6) were significantly different (p<0.05), but the internal fits (2, 3, 4, 5) were not significantly different (p>0.05). These results show that Ni-Cr alloys should not be processed bythe heat treatmentmethod.However, they need to be confirmed in further clinical application studies.

Development of a new lifetime prediction method for gas turbine core parts by digital image analysis of precipitates morphology (석출물 형상의 디지털 이미지 분석에 의한 가스터빈 핵심부품의 새로운 수명평가기술 개발)

  • Chang, Moon Soo;An, Seong Uk
    • Analytical Science and Technology
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    • v.21 no.2
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    • pp.148-157
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    • 2008
  • To describe the lifetime prediction of gas turbine core parts serviced in some ten thousands rpms at over $1,000^{\circ}C$, the Larson-Miller Creep Curves, which are formed by creep rupture tests as the destructive experiment with parameters of stress and temperature, are used often, but not exact and reliable with errors of over some tens. On the other hand, this study shows a non-destructive method with increased accuracy and reliability. The SEM and TEM specimens were extracted by replica after polishing the local airfoil and root surfaces of the first stage scraped blade (bucket), serviced for 18,000 hours at $1,280^{\circ}C$ in Gas Turbines of Boryong. The observed TEM and SEM precipitates were digitalized for calculation of the average size. Here we could find the precipitate size grown from $0.45{\mu}m$ to $0.6{\mu}m$ during service and the grown precipitates to be still sound. From these results we could conclude that the scraped balde can be used for ten thous and hours additionally and for twenty thousand hours by additional heat treatments on the scraped blade.

Methods of Merging a 3D Replica and Ease Distribution for Woman's Pant Patterns (여성용 바지 패턴 설계를 위한 3차원 밀착 패턴 여유량 부가와 레플리카 조합 방법)

  • Wu, Yanjun;Hong, Kyung-Hi
    • Journal of the Korean Society of Clothing and Textiles
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    • v.36 no.4
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    • pp.443-455
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    • 2012
  • In this research, a woman matched to the average Korean standard size specification of 21-24 years of age was scanned using Cyberware. Rapidform 2004 was used to separate the body zone into fit zone, action zone and design zone, depending on the function of the lower body. Each divided body zone expanded radially using 'offset' by 1.60mm at the cross section of the waist, and 6.36mm at the hip level. Resultant ease values were 1cm and 4cm along the waistline and hipline (respectively), as recommended in previous research. 2C-AN program and Yuka CAD was used to develop a pant pattern from the enlarged 3D body surface blocks. A total of five pants were constructed using conventional fabrics for light weight formal pants. The appearance of the experimental pants were evaluated by five clothing-major evaluators using a questionnaire that consisted of 21 questions. In addition, functional aspects of the experimental pants were also evaluated by wearer while performing five postures for the measurement of ease of movement. It was found that, the method of regional offset and suggested 3D to 2D pattern development generate reasonably good pant patterns. Among the pattern block arrangements, the method B was evaluated as the best way to generate formal pants from the tight-fit pattern, which fit smoothly over the lower body for maintaining ease of movement.

Effect of HAZ Softening Zone on Creep Rupture Properties of 1.0Cr-1.0Mo-0.25V Turbine Steels -Part II : Carbide Morphology- (1.0Cr-1.0Mo-0.25V 터어빈 로터강의 열영향부 연화층이 크립 파단 특성에 미치는 영향 - Part II : 탄화물 형태 -)

  • ;Indacochea, J. E.
    • Journal of Welding and Joining
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    • v.15 no.1
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    • pp.101-108
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    • 1997
  • In repaired weldment of ASTM A-470 class 8 high pressure stream turbine rotor steel, creep rupture life was studied in relation with carbide morphology. Carbides were identified using carbide extraction replica method. A retired rotor has molybdenum rich carbide $M_2C$, lndacochea vanadium rich carbide $M_4C_3$, and chromium rich carbides $M_{23}C_6$and $M_7C_3$. Weldments ruptured at ICHAZ showed that some of carbides have been transformed into spherical types of coarsened carbides at ruptured area. Those carbides were revealed as molybdenum rich $M_6C$ carbide and they provided cavitation sites due to molybdenum depletion around $(M_6C)$ carbide. However coarsened $M_6C$ and $M_{23}C_6$ carbides were observed at ruptured area in case of ruptured at CGHAZ.

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Adaptive Fuzzy Drop Manager for Service of Reliable Distribution Application Domain Objects (신뢰성 있는 분산 도메인 객체 서비스를 위한 적응형 퍼지 드럽 관리기)

  • Jeong, Taeg-Won;Lee, Chong-Deuk
    • Journal of the Korean Institute of Intelligent Systems
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    • v.19 no.4
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    • pp.511-518
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    • 2009
  • A lot of methods are proposed to provide services for object informations in distributed domain to satisfy the recent increase of user-centered services. This paper proposed a method called fuzzy drop manager for the service of reliable distribution application domain objects. The proposed system accesses the domain using replica parameter ci,j and access matrix Z, and evaluates the reference relatedness inside the domain using the relatedness, given by the mapping of intra-domain fuzzy relevance, between fuzzy sets. Objects in the domain generated an $\alpha$-level set according to the reference relatedness obtained by applying $\alpha$-level to extend queries. Simulation results showed that the proposed method has better performance than the other methods.

Modeling of 3D Monte Carlo Ion Implantation in the Ultra-Low Energy for the Fabrication of Giga-Bit Devices (기가 비트급 소자 제작을 위한 3차원 몬테카를로 극 저 에너지 이온 주입 모델링)

  • Ban, Yong-Chan;Kwon, Oh-Seob;Won, Tae-Young
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.37 no.10
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    • pp.1-10
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    • 2000
  • A rigorous modeling of ultra-low energy implantation is becoming increasingly more important as devices shrink to deep submicron dimensions. In this paper, we have developed an efficient three-dimensional Monte Carlo ion implantation model based on a modified Binary Collision Approximation(BCA). To this purpose, the modified electronic stopping model and the multi-body collision model have been taken into account in this simulator. The dopant and damage profiles show very good agreement with SIMS(Secondary Ion Mass Spectroscopy) data and RBS(Rutherford Backscattering Spectroscopy) data, respectively. Moreover, the ion distribution replica method has been implemented into the model to get a computational efficiency in a 3D simulation, and we have calculated the 3D Monte Carlo simulation into the topographically complex structure.

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