• Title/Summary/Keyword: repetitive sequence

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Five Computer Simulation Studies of Whole-Genome Fragment Assembly: The Case of Assembling Zymomonas mobilis ZM4 Sequences

  • Jung, Cholhee;Choi, Jin-Young;Park, Hyun Seck;Seo, Jeong-Sun
    • Genomics & Informatics
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    • v.2 no.4
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    • pp.184-190
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    • 2004
  • An approach for genome analysis based on assembly of fragments of DNA from the whole genome can be applied to obtain the complete nucleotide sequence of the genome of Zymomonas mobilis. However, the problem of fragment assembly raise thorny computational issues. Computer simulation studies of sequence assembly usually show some abnormal assemblage of artificial sequences containing repetitive or duplicated regions, and suggest methods to correct those abnormalities. In this paper, we describe five simulation studies which had been performed previous to the actual genome assembly process of Zymomonas mobilis ZM4.

The complete chloroplast genome of Campsis grandiflora (Bignoniaceae)

  • PARK, Jongsun;XI, Hong
    • Korean Journal of Plant Taxonomy
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    • v.52 no.3
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    • pp.156-172
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    • 2022
  • Campsis grandiflora (Thunb.) K. Schum is an ornamental species with various useful biological effects. The chloroplast genome of C. grandiflora isolated in Korea is 154,293 bp long (GC ratio: 38.1%) and has four subregions: 84,121 bp of large single-copy (36.2%) and 18,521 bp of small single-copy (30.0%) regions are separated by 24,332 bp of inverted repeat (42.9%) regions including 132 genes (87 protein-coding genes, eight rRNAs, and 37 tRNAs). One single-nucleotide polymorphism and five insertion and deletion (INDEL) regions (40-bp in total) were identified, indicating a low level of intraspecific variation in the chloroplast genome. All five INDEL regions were linked to the repetitive sequences. Seventy-two normal simple sequence repeats (SSRs) and 47 extended SSRs were identified to develop molecular markers. The phylogenetic trees of 29 representative Bignoniaceae chloroplast genomes indicate that the tribe-level phylogenic relationship is congruent with the findings of previous studies.

Efficient Stream Sequence Matching Algorithms for Handheld Devices over Time-Series Stream Data (시계열 스트림 데이터 상에서 핸드헬드 디바이스를 위한 효율적인 스트림 시퀀스 매칭 알고리즘)

  • Moon Yang-Sae;Loh Woong-Kee
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.31 no.8B
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    • pp.736-744
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    • 2006
  • For the handhold devices, minimizing repetitive CPU operations such as multiplications is a major factor for their performances. In this paper, we propose efficient algorithms for finding similar sequences from streaming time-series data such as stock prices, network traffic data, and sensor network data. First, we formally define the problem of similar subsequence matching from streaming time-series data, which is called the stream sequence matching in this paper. Second, based on the window construction mechanism adopted by the previous subsequence matching algorithms, we present an efficient window-based approach that minimizes CPU operations required for stream sequence matching. Third, we propose a notion of window MBR and present two stream sequence matching algorithms based on the notion. Fourth, we formally prove correctness of the proposed algorithms. Finally, through a series of analyses and experiments, we show that our algorithms significantly outperform the naive algorithm. We believe that our window-based algorithms are excellent choices for embedded stream sequence matching in handhold devices.

Dissemination of CTX-M Type Extended-Spectrum β-Lactamases Among Klebsiella pneumoniae Clinical isolates in Chungcheong Province (충청지역의 임상검체에서 분리된 폐렴막대균에 CTX-M형 Extended-Spectrum β-lactamases 확산)

  • Sung, Ji-Youn
    • Journal of Digital Convergence
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    • v.14 no.10
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    • pp.349-354
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    • 2016
  • The emergence and dissemination of extended-spectrum ${\beta}$-lactamse (ESBL) producing Klebsiella pneumoniae isolates make it more difficult to treatment of bacterial infections. In our study, we detected ESBL genes and investigated antimicrobial susceptibility of K. pneumoniae isolates in Chungcheong province. In addition, clonality among the isolates was analyzed by repetitive element sequence (REP)-PCR. Twenty-one of 102 K. pneumoniae isolates produced CTX-M-14 and/or CTX-M-15 and showed high level (over 70%) resistance to third cephalosporins. CTX-M type ESBL producing K. pneumoniae strains isolated in our study showed diverse clonality and some of the isolates have been disseminated in the community. Enhancing infection control will be needed to prevent dissemination of the K. pneumoniae isolates. In addition, for more effective control of resistant bacteria it is considered necessary to monitor the database constructed through convergence of biological investigation and statistical analysis of antimicrobial resistance genes.

Genetic Diversity of Ralstonia solanacearum Strains Isolated from Pepper and Tomato Plants in Korea (우리나라에 분포하는 고추와 토마토 풋마름병균(Ralstonia solanacearum) 계통들의 유전적 다양성)

  • Seo, Sang-Tae;Park, Jong-Han;Han, Kyoung-Suk;Cheong, Seung-Ryong;Lee, Seung-Don
    • Research in Plant Disease
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    • v.13 no.1
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    • pp.24-29
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    • 2007
  • A total of 35 strains of Ralstonia solanacearum isolated from wilted pepper and tomato plants in Korea were analyzed for their genetic diversity by bacteriological, pathological and molecular biological approaches. All the strains were identified as R. solanacearum biovar 4 on the basis of physiological and biochemical tests, and species-specific PCR primers. Pathogenicity of the strains was confirmed by inoculating on 4-week-old pepper and tomato seedlings. Using cluster analysis based on repetitive sequence-based polymerase chain reaction (rep-PCR) genomic fingerprints, R. solanacearum strains isolated from pepper and tomato in Korea divided into 6 groups showing a high degree of genetic diversity at 55% similarity level. The genetic diversify of strains was not significantly correlated with their geographic origins and host plants.

Late Pleistocene Fluvial Sequence in South Korea

  • Kim, Ju-Yong;Yang, Dong-Yoon;Nahm, Wook-Hyun;Lee, Yung-Jo;Park, Ji-Hoon
    • The Korean Journal of Quaternary Research
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    • v.18 no.2 s.23
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    • pp.33-42
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    • 2004
  • In South Korea a Pedo-sedimentary Sequence(PS) indicating the Last Glacial Maximun(LGM) is typified y a brown to dark brown, relatively stiff paleosol layers formed by repetitive freezing and thawing processes which in turn left characteristi glossic textures in soil-solum, polygolnal structures with a flagipans, vertical soil wedges or freezing cracks, and horizontal foliations, As a pre-LGM sedimentary sequences (older than 25Ka), the Old Fluvial Sequence(OFS) overlain by the Slope Sedimentary Sequence(SS) are distributed commonly at the base level higher than 14-15m above present river-bed along the major river basin. After the LGM (ca. 18Ka), the Young Fluvial Sequence(YFS) appears at an altitude ascending order of sedimentary profiles. In this fluvial organic muds of Jangheungri site(Jinju), Sorori site(Cheonwon), and Youngsan estruarine rivermouth(Mokpo) were exemplified in order to interpret their formation ages and environments. As result of $^{14}C$ datings, the formation ages of te organic muds are Boelling to Alleroed (MIS-1). These organic muds were fomed in fluvial backswamp or local pond/bog in response to shifting fluvial system. On the basis of palynological production dominant with Abies/Picea-Betula and Ranunculaceae, Compositae, Cyperaceae, and Graminae, it was interpreted that more boreal to subboreal condition was prevailed rather than temperate like today during the formation of organic muds and soil moisture condition was a repetition of wet and dry condition.

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Analyzing and Designing a Current Controller for Circulating Current Reduction in Parallel Three-Phase Voltage-Source Inverters

  • Kim, Kiryong;Shin, Dongsul;Kim, Hee-Je;Lee, Jong-Pil
    • Journal of Power Electronics
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    • v.18 no.2
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    • pp.502-510
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    • 2018
  • A circulating current is a major problem caused by directly connecting voltage-source inverters (VSIs) in parallel. This circulating current occurs as a zero-sequence current between the inverters by specific switch states. Several studies have presented alternatives using hardware and software methods. When coupled inductors (CIs) are employed for the high-frequency circulating current, a controller is required to prevent the low-frequency circulating current from saturating the CIs. In this study, the zero-sequence circulating current and its alternatives are investigated using hardware and mathematical description. A high-performance circulating current controller is proposed by applying a repetitive controller to the zero-sequence current control loop. The proposed controller can effectively minimize the low-frequency circulating current without any data sharing between the inverters in unfavorable conditions. It can also be applicable to the modular configuration of parallel three-phase VSIs. Experimental results verify the performance of the proposed controller.

Effect of rTMS on Motor Sequence Learning and Brain Activation : A Preliminary Study (반복적 경두부 자기자극이 운동학습과 뇌 운동영역 활성화에 미치는 영향 : 예비연구)

  • Park, Ji-Won;Kim, Jong-Man;Kim, Yun-Hee
    • Physical Therapy Korea
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    • v.10 no.3
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    • pp.17-27
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    • 2003
  • Repetitive transcranial magnetic stimulation (rTMS) modulates cortical excitability beyond the duration of the rTMS trains themselves. Depending on rTMS parameters, a lasting inhibition or facilitation of cortical excitability can be induced. Therefore, rTMS of high or low frequency over motor cortex may change certain aspects of motor learning performance and cortical activation. This study investigated the effect of high and low frequency subthreshold rTMS applied to the motor cortex on motor learning of sequential finger movements and brain activation using functional MRI (fMRI). Three healthy right-handed subjects (mean age 23.3) were enrolled. All subjects were trained with sequences of seven-digit rapid sequential finger movements, 30 minutes per day for 5 consecutive days using their left hand. 10 Hz (high frequency) and 1 Hz (low frequency) trains of rTMS with 80% of resting motor threshold and sham stimulation were applied for each subject during the period of motor learning. rTMS was delivered on the scalp over the right primary motor cortex using a figure-eight shaped coil and a Rapid(R) stimulator with two Booster Modules (Magstim Co. Ltd, UK). Functional MRI (fMRI) was performed on a 3T ISOL Forte scanner before and after training in all subjects (35 slices per one brain volume TR/TE = 3000/30 ms, Flip angle $60^{\circ}$, FOV 220 mm, $64{\times}64$ matrix, slice thickness 4 mm). Response time (RT) and target scores (TS) of sequential finger movements were monitored during the training period and fMRl scanning. All subjects showed decreased RT and increased TS which reflecting learning effects over the training session. The subject who received high frequency rTMS showed better performance in TS and RT than those of the subjects with low frequency or sham stimulation of rTMS. In fMRI, the subject who received high frequency rTMS showed increased activation of primary motor cortex, premotor, and medial cerebellar areas after the motor sequence learning after the training, but the subject with low frequency rTMS showed decreased activation in above areas. High frequency subthreshold rTMS on the motor cortex may facilitate the excitability of motor cortex and improve the performance of motor sequence learning in normal subject.

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Molecular characterization of a repetitive element of Xanthomonas oryzae pv. oryzae

  • Yun, Choong-Hyo
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 1995.06b
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    • pp.1-19
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    • 1995
  • The plasmid pJEL 101 contains a highly repetitive element from the genome of Xanthomonas oryae pv. oryzae that has properties of an insertional element. The insertional nature of the element, hereto referred to as IS203, was confirmed by molecular analyses of the element and three related elements that were isolated from X. oryzae. The related sequences were isolated on the basis of transposition to the transposon-trapping vector pL3SAC and hybridization with pJEL101. The trapped elements (IS203a, IS203b, and IS203c) were each composed of 1,055 base pairs with 25 base terminal inverted repeats. The elements caused a three base pair target site duplication at the site of insertion in the sacRB gene. The sequence of pJEL 101 has 96% base pair identity with IS203a and 99% identity with IS203a and IS203c but lacks three nucleotides of the consensus left terminal repeat. IS203b has the same DNA sequences as IS203c but is inserted ito the sacRB gene in the opposite orientation. The longest open reading frame of IS203a could code for a protein of 318 amino acids and molecular weight of 37, 151. A search of the Genbank database revealed that IS203 has 51% identity with 909 nucleotides of IS4551 from Escherichia coli. The predicted protein of ORF1 has 40% and 30% amino acid identity to the ORF1 of Tn4551 and the transposase of IS30, respectively.

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