• ALGAE
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• v.36 no.4
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• pp.315-326
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• 2021

Ion channels are membrane protein complexes mediating passive ion flux across the cell membranes. Every organism has a certain set of ion channels that define its physiology. Dinoflagellates are ecologically important microorganisms characterized by effective physiological adaptability, which backs up their massive proliferations that often result in harmful blooms (red tides). In this study, we used a bioinformatics approach to identify homologs of known ion channels that belong to 36 ion channel families. We demonstrated that the versatility of the dinoflagellate physiology is underpinned by a high diversity of ion channels including homologs of animal and plant proteins, as well as channels unique to protists. The analysis of 27 transcriptomes allowed reconstructing a consensus ion channel repertoire (channelome) of dinoflagellates including the members of 31 ion channel families: inwardly-rectifying potassium channels, two-pore domain potassium channels, voltage-gated potassium channels (K_v), tandem K_v, cyclic nucleotide-binding domain-containing channels (CNBD), tandem CNBD, eukaryotic ionotropic glutamate receptors, large-conductance calcium-activated potassium channels, intermediate/small-conductance calcium-activated potassium channels, eukaryotic single-domain voltage-gated cation channels, transient receptor potential channels, two-pore domain calcium channels, four-domain voltage-gated cation channels, cation and anion Cys-loop receptors, small-conductivity mechanosensitive channels, large-conductivity mechanosensitive channels, voltage-gated proton channels, inositole-1,4,5-trisphosphate receptors, slow anion channels, aluminum-activated malate transporters and quick anion channels, mitochondrial calcium uniporters, voltage-dependent anion channels, vesicular chloride channels, ionotropic purinergic receptors, animal volage-insensitive cation channels, channelrhodopsins, bestrophins, voltage-gated chloride channels H⁺/Cl^- exchangers, plant calcium-permeable mechanosensitive channels, and trimeric intracellular cation channels. Overall, dinoflagellates represent cells able to respond to physical and chemical stimuli utilizing a wide range of G-protein coupled receptors- and Ca²⁺-dependent signaling pathways. The applied approach not only shed light on the ion channel set in dinoflagellates, but also provided the information on possible molecular mechanisms underlying vital cellular processes dependent on the ion transport.

• The Journal of the Korea Contents Association
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• v.18 no.12
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• pp.372-382
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• 2018

Understanding is the foundation of music appreciation, the ultimate goal of music learning. The purpose of this research was to create a model for the integration and implementation of Gordon's music learning theory principles into Suzuki violin instruction. This research shows how audiation can be taught within the framework of the Suzuki philosophy and curriculum. It is expected that Gordon's music learning theory can provide Suzuki teachers with unique capabilities for teaching musical understanding, specifically through the development of audiation. Through an audiation-based approach to Suzuki instruction, thus, young violinists will be able to learn to comprehend the tonal and rhythmic aspects of the music they perform. Future researchers may wish to assemble a more complete guide to the tonal and rhythm patterns used in the Suzuki repertoire. Furthermore, additional lesson plans and activities can and should be developed that represent the application of music learning theory to Suzuki violin instruction.

• Cross-Cultural Studies
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• v.25
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• pp.421-452
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• 2011

This writing is about an effort to try to preserve and transmit the materials related to Jing-Ju(京劇) who is the Chinese traditional theatrical art. The plan to preserve the materials of performing arts is very important because its collection and preservation about them are much more particular than other genres. China got the great results in this field because of China Jing-Ju Yinpeixiang (中國京劇音配像) project. Jing-Ju Yinpeixiang project is a kind of project to restore and preserve the materials which reproduced Jing-Ju repertoire by inserting the performance method of Yinpeixiang (音配像: put together images to sounds) by later actors and actresses of Jing-Ju in recordings of 1950s to 1960s by remained famous ones. Also, this is the large scale culture project that more than 170 institutions and more than 3000 persons participated in during the total 21 years that it first started in 1985 and was completed in the end of 2006. Thanks to this project, China got to have the almost perfect materials which reproduced live performances to add the images of junior actors and actresses who accurately imitated performances of 115 famous actors and actresses including each type of role, schools and great actors from 1907 to the end of 20th century in their voices. This is the only method to restore the materials in this world and very creative. It's been 15 years since TV broadcasting first showed its fruition to Chinese people. The Chinese government gave the positive judgement on this project and prepared to hold the big event which celebrated itself this year. But very little is known of this process and there is no any study in Korea. This writing tried to clarify what Jing-Ju Yinpeixiang project is, what it has meant to Chinese people and what results it has gotten after it's been 15 years since the first show was on TV.

• Korean Journal of Agricultural Science
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• v.48 no.3
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• pp.643-654
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• 2021

Phytophthora capsici is one of the most destructive hemibiotrophic pathogens; it can cause blight in chili peppers, and secrete various effector proteins to infect the plants. These effectors contain an N-terminal conserved RXLR motif. Here, we generated full-length RXLR effector coding genes using primer pairs, and cloned them into the pGR106 vector for in planta expression. Two of these genes, PcREK6 and PcREK41 (P. capsici RXLR effector from the Korea isolate), were further characterized. PcREK6 and PcREK41 genes showed that they encode effector proteins with a general modular structure, including the N-terminal conserved RXLR-DEER motif and signal peptide sequences. PcREK6 and PcREK41 expressions were strongly induced when the chili pepper plants (Capsicum annuum) were challenged with P. capsici. These results provide molecular evidence to elucidate the virulence or avirulence factors in chili pepper. Our results also showed that two effectors induce hypersensitive response (HR) cell death when expressed in chili leaves. Cell death suppression assays in Nicotiana benthamiana revealed that most effectors could not suppress programmed cell death (PCD) triggered by Bcl-associated X (BAX) or Phytophthora infestans elicitin (INF1). However, PcREK6 fully suppressed PCD triggered by BAX, while PcREK41 partially suppressed PCD triggered by INF1 elicitin. These results suggest that PcREK effectors from P. capsici interact with putative resistance (R) proteins in planta, and different effectors may target different pathways in a plant cell to suppress pattern-triggered immunity (PTI) or effector-triggered immunity (ETI).

• Journal of Mushroom
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• v.19 no.1
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• pp.33-40
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• 2021

The genome sequence of various Flammulina species has recently been reported, thereby revealing a diverse genetic repertoire. In this study, we identified laccase genes and analyzed their structural characteristics in Flammulina elastica (F. elastica) genome. Through genome analysis and bioinformatics approaches, three laccase genes (Fe-lac1, -lac2, and -lac3) were identified, ranging from 1,548 to 1,602 bp in length. These genes contained a copper ion-binding region with ten histidine residues and one cysteine residue and a disulfide bond-forming region with four cysteine residues. Full-length cDNA sequencing analysis revealed that laccase genes contain 12 to 16 introns and signal peptides between 17 and 22 bp in the N-terminus. Structural characterization of the laccase genes identified in this study should help in better understanding the biomass decomposition of F. elastica.

• The Journal of the Korea Contents Association
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• v.21 no.12
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• pp.132-139
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• 2021

This corpus-driven research expounded the compilation of the most frequently occurring academic words in the domain of syntax and compared the extracted wordlist with Academic Word List(AWL) of Coxhead(2000) and General Service List(GSL) of West(1953) to examine their distribution and coverage within the syntax corpus. A specialized 546,074 token corpus, composed of widely used must-read syntax textbooks for English education majors, was loaded into and analyzed with AntWordProfiler 1.4.1. Under the parameter of lexical frequency, the analysis identified 288(50.5%) AWL word forms, appeared 16 times or more, as well as 218(38.2%) AWL items, occurred not exceeding 15 times. The analysis also indicated that the coverage of AWL and GSL accounted for 9.19% and 78.92% respectively and the combination of GSL and AWL amounted to 88.11% of all tokens. Given that AWL can be instrumental in serving broad disciplinary needs, this study highlighted the necessity to compile the domain-specific AWL as a lexical repertoire to promote academic literacy and competence.

• Journal of Animal Science and Technology
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• v.63 no.6
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• pp.1423-1432
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• 2021

To elucidate the role and mechanism of microbes, we combined culture-dependent and culture-independent approaches to investigate differences in gut bacterial composition between sows and weaned pigs. Under anaerobic conditions, several nonselective and selective media were used for isolation from fecal samples. All isolated bacteria were identified and classified through 16S rRNA sequencing, and the microbiota composition of the fecal samples was analyzed by metagenomics using next generation sequencing (NGS) technology. A total of 278 and 149 colonies were acquired from the sow and weaned pig fecal samples, respectively. Culturomics analysis revealed that diverse bacterial genus and species belonged to Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes were isolated from sow and weaned pigs. When comparing culture-dependent and culture-independent analyses, 191 bacterial species and 2 archaeal bacterial species were detected through culture-independent analysis, and a total of 23 bacteria were isolated through a culture-dependent approach, of which 65% were not detected by metagenomics. In conclusion, culturomics and metagenomics should be properly combined to fully understand the intestinal microbiota, and livestock-derived microbial resources should be informed by culturomic approaches to understand and utilize the mechanism of host-microbe interactions.

• (The) Research of the performance art and culture
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• no.41
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• pp.39-67
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• 2020

So far, research on the 10th performance of the Geukye Suryeonguhoe has been rarely conducted. In particular, Lee Seo-hyang's Eomeoni was rarely studied, and it was only recorded that at most it was one of the 10th performances. Although the script of Eomeoni remains, and the 10th performance was an important regular performance, it should be said that the status of this research is very poor. It seems to be the result of failing to accurately examine the historical value and meaning of the single act drama Eomeoni. In order to solve these problems, it is necessary to look at the performance situation of Eomeoni and to investigate the situation at the time of Geugyesuryeonguhoe. In addition, the reason for selecting Eomeoni, the stage performance situation, theatrical elements, stage design, and related evaluations should be analyzed throughout. This study focused on examining the status of Eomeoni's performance and examining its significance, and intends to examine the flow of feminist play as a context of the Geeugyesuryeonguhoe through the conclusion.

• Parasites, Hosts and Diseases
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• v.61 no.1
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• pp.24-32
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• 2023

Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), encoded by the polymorphic var multigene family, is a highly polymorphic antigen that plays a crucial role in the pathology of malaria. The contribution of the genetic diversity of var toward the immune escape of P. falciparum has not yet been fully elucidated. This study aimed to characterize the diversity of var repertoires by screening P. falciparum Duffy-binding-like α domain (PfDBLα) among field isolates from central Myanmar. Genetic analysis revealed that the D-H segments of var in Myanmar populations have an extensive polymorphic repertoire, with high numbers of unique sequence types in each individual. However, var genes from the global population, including Myanmar, shared close genetic lineages regardless of their geographic origins, indicating that they have not undergone rapid evolutionary changes.

• Korean Journal of Culture and Social Issue
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• v.10 no.spc
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• pp.69-86
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• 2004

A review of literature revealed that damaged self-confidence of men as social agents may be the primary, if not proximal, cause of domestic violence. Accumulated damages in social confidence and self-assurance may be moderated by action repertoire acquired during childhood, and mediated by adulthood circumstances such as marital discords and the lack of social support to result in the typical cycle of violence and subsequent feeling of remorse. The present treatments for the domestically violent men in Korea seem to be ineffective to reduce the number of incidents in the society because the treatments are viewed as punishments by the men, damage their social confidence further by stigmatizing them in the community, and destroy their social resources and support systems. It was suggested in this paper to reduce the role of law enforcement and correctional administration to rehabilitate the currently violent men. At the same time, it was also suggested for the Korean court to implement the paradigm of Therapeutic Jurisprudence in handling domestic violence cases. It was argued that the court should take active roles as a healing and rehabilitating agent by cooperating with non-government community establishments such as hospitals, universities and self-help organizations. The reasons and implications of those suggestions were discussed in detail.