• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.34 no.5C
• /
• pp.555-560
• /
• 2009

A vehicle radar has been studied to avoid the collision of vehicles. A vehicle radar using direct sequence-ultra wideband (DS-UWB) measures the distance between a vehicle and an objective with a sufficient time margin. Therefore, the receiver should estimate the distance by processing the short UWB pulse rapidly. There have been several investigations for distance measuring based on DS-UWB signals. In this paper, we propose a novel method for rapid measuring processing time. Simulation results show that the proposed method has a shorter processing time than the conventional method.

• Investigative Magnetic Resonance Imaging
• /
• v.23 no.4
• /
• pp.390-394
• /
• 2019

Hemosiderosis is characterized by the deposition of excess iron in body tissues. The choroid plexus is an important part of the central nervous system that can be the primary site of iron overload. T2*-weighted gradient echo (GRE) sequence provides high sensitivity for demonstrating cerebral microhemorrhagic foci and iron deposition. In the present study, we describe the case of a 15-year-old boy with acute lymphoblastic leukemia, in whom repeated transfusion led to iron accumulation in the brain. GRE sequence effectively demonstrated hemosiderin deposition in the choroid plexus.

• Journal of the Institute of Electronics Engineers of Korea TC
• /
• v.42 no.12
• /
• pp.117-126
• /
• 2005

In this paper, we present a new method for the reduction of the PAPR (peak to average power ratio) of OFDM signals. The idea behind the proposed method is that IFFT is implemented often with software for a digital signal processor such that we may avoid the repeated calculations to reduce the computational operations: we define sub-sequences in the IFFT process and then multiply the optimum phase rotation factors to them to minimize the PAPR. The PAPR reduction performance of the proposed method is equal to that of the interleaved partition scheme of the PTS (partial transmit sequence) method with only 1/3 computational operations of it.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.32 no.2A
• /
• pp.169-178
• /
• 2007

In this paper, we propose a signal structure and its optimum receiver to improve performance of SC-FDE(Single Carrier with Frequency Domain Equalization) system. Conventional SC-FDE systems have a drawback of power unbalance in frequency domain due to generation of pilot signals in time domain. The unbalanced power in frequency domain induces a channel estimation error and the performance of the receiver is degraded significantly. To overcome the drawback we apply CAZAC sequence which has constant power distribution in time and frequency domain. We design the signal structure to improve the performance with the repeated CAZAC sequence, and we design a receiver to optimize the proposed structure. Computer simulation results show that the proposed structure is superior to the conventional structure considering frame synchronization, frequency synchronization and channel equalization on typical wireless mobile channel environment.

• Mycobiology
• /
• v.48 no.2
• /
• pp.122-132
• /
• 2020

Citric acid is a commercially valuable organic acid widely used in food, pharmaceutical, and beverage industries. In this study, 260 yeast strains were isolated from soil, bread, juices, and fruits wastes and preliminarily screened using bromocresol green agar plates for their ability to produce organic acids. Overall, 251 yeast isolates showed positive results, with yellow halos surrounding the colonies. Citric acid production by 20 promising isolates was evaluated using both free and immobilized cell techniques. Results showed that citric acid production by immobilized cells (30-40 g/L) was greater than that of freely suspended cells (8-19 g/L). Of the 20 isolates, two (KKU-L42 and KKU-L53) were selected for further analysis based on their citric acid production levels. Immobilized KKU-L42 cells had a higher citric acid production rate (62.5%), while immobilized KKU-L53 cells showed an ~52.2% increase in citric acid production compared with free cells. The two isolates were accurately identified by amplification and sequence analysis of the 26S rRNA gene D1/D2 domain, with GenBank-based sequence comparison confirming that isolates KKU-L42 and KKU-L53 were Candida tropicalis and Pichia kluyveri, respectively. Several factors, including fermentation period, pH, temperature, and carbon and nitrogen source, were optimized for enhanced production of citric acid by both isolates. Maximum production was achieved at fermentation period of 5 days at pH 5.0 with glucose as a carbon source by both isolates. The optimum incubation temperature for citric acid production by C. tropicalis was 32 ℃, with NH₄Cl the best nitrogen source, while maximum citric acid by P. kluyveri was observed at 27 ℃ with (NH₄)₂ SO₄ as the nitrogen source. Citric acid production was maintained for about four repeated batches over a period of 20 days. Our results suggest that apple and banana wastes are potential sources of novel yeast strains; C. tropicalis and P. kluyveri which could be used for commercial citric acid production.

• Journal of Mushroom
• /
• v.14 no.4
• /
• pp.174-178
• /
• 2016

Simple sequence repeats (SSR), also referred to "microsatellites" consist of tandemly repeated short DNA sequence motifs and have been applied in various marker-based studies. SSRs were isolated and characterized from 'Heuktari' and 'Miso', which are major oyster mushroom cultivars in Korea, by genome sequencing and bioinformatic analysis. The genome sizes of 'Heuktari' and 'Miso' were estimated to be 40.8 and 40.3 Mb, respectively, which are larger than those of other P. ostreatus species (PC9 and PC10) and smaller than those of P. eryngii (KNR2312P5). In total, 949 and 968 SSRs were found in the 'Heuktari' and 'Miso' genomes, respectively. Comparative analysis of five mushrooms including P. ostreatus var. florida (PC9 and PC15) and P. eryngii revealed that the number of SSRs in 'Heuktari' and 'Miso' were the highest among them. All mushrooms studied showed similar SSR distribution patterns. Tri-, hexa-, and octanucleotide motifs accounted for the top three fractions of all SSRs.

• Proceedings of the Korean Society of Sericultural Science Conference
• /
• 1997.06a
• /
• pp.73-101
• /
• 1997

Baculoviruses are characterized by large double-stranded circular DNA genomes and rod-shaped enveloped virions. Bombyx mori nucleopolyhedrovirus(BmNPV) is a major pathogen, which causes severe damage in sericulture. Currently, BmNPV is recogtnized as an improtant tool in molecular biology, especially for expression of useful genes in B.mori cells and silkworm larvae. Our laboratories have focused on the studies of the molecular mechanisms of BmNPV replication and the application of BmNPV to agriculture and medicine. The entire nucleotide sequence of the BmNPV genome has recently determined. The BmNPV genome possessed 135 putative genes and 7 homologous repeated sequence (hrs) regions. Relatively little space, a few to a few hundred base-pairs, was observed between the open reading frames and hrs. Termination codons often overlapped. These results showed a compactly packde BmNPV genome. Based on comparative sequence analyses, we speculated that the ancestor of BmNPV was a baculovirus similar to Autographa californica NPV(AcNPV). The function of the BmNPV genes were characterized by gene deletion analysis; p35 was found to be involved in blocking apoptosis and cysteine proteinase was found to be involved in horizontal virus transmission by degrading viral-infected larval host. By AcNPV and BmNPV coinfection experiments, we identified a BmNPV gene involved in expanding host specificity of AcNPV. The identified gene was likely encoded a DNA helicase based on the amino acid sequence analysis; a few amino acid substitutions in the putative DNA helicase gene resulted in the expansion of host range of AcNPV. These findings indicate that BmNPV evolved within a short period from an AcNPV-like ancestral virus due to rapid evolution including specific amino acid substitutions and gene deletions/insertions.

• Journal of Microbiology and Biotechnology
• /
• v.11 no.3
• /
• pp.515-523
• /
• 2001

The dinoflagellates have some primitive nuclear features and are evolutionarily intermediate between prokaryotes and eukaryotes. The small subunit ribosomal RAN gene, the 5.8S ribosomal RNA gene, and the internal transcribed spacer (ITS) of Gonyaulax polyedra were cloned, and their sequences were analyzed to better understand their evolutionary position. The small subunit ribosomal RNA gene was 1,794 nt long, the large subunit ribosomal RNA gene was approximately 3,500 nt long, and the 5.8S ribosomal RNA gene was 159 nt long. The first internal transcribed spacer (ITS1) was 191 nt long, and the second internal transcribed spacer (ITS2) was 185 nt long. The intergenic spacer of the ribosomal RNA gene (IGS) was about 2,200 nt long, indicating that 5,800 nt of transcribed sequences were separated by roughly 2,200 nt of intergenic spacer. The ribosomal RNA genes were repeated many times and arranged in a head-to-tail, tandemly repeated manner. The repeating unit of ribosomal RNA gene of G. polyedra was proposed to be 8,000 nt long. Based on the lengths of ribosomal RNA, sequence alignments with representative organisms, and phylogenetic analysis on ribosomal RNA, G. polyedra appears to be one of the alveolates branched from the eukaryotic crown and, among dinoflagellates, it seems to not have emerged early.

• Journal of Microbiology and Biotechnology
• /
• v.10 no.3
• /
• pp.312-320
• /
• 2000

Abstract The full sequence of the plasmid pKJ36, which was derived from Bifidobacterium longum KJ, was determined and analyzed to construct shuttle vectors between E. coli and Bifidobacterium. The plasmid pKJ36 was composed of 3,625 base pairs with a 65.1% G+C content. The structural organization of pKJ36 was highly similar to that of pKJ50, and the three major ORFs on pKJ36 showed high amino acid sequence homologies with those of pKJ50. The putative proteins coded by these three ORFs were designated as RepB (32.0 kDa, pI=9.25), MembB (29.0 kDa, pI=12.25), and MobB (39.0 kDa, pI=IO.66), respectively. The amino acid sequence of RepB showed a 57% identity and 70% similarity with that of the RepA protein of pKJ50. Upstream of the repB gene, the so-called iteron sequence was directly repeated four-and-ahalf times and a conserved dnaA box was identified. An amino acid sequence comparison between the MobB and MobA of pKJ50 revealed a 48% identity and 61 % similarity. A conserved oriT sequence with an inverted repeat identical to that of pKJ50 was also found upstream of the mobB gene. A hydropathy analysis of MembB revealed four possible transmembrane regions. The expressions of the repB and membB genes were confirmed by RT-PCR. The in vitro translation reaction of pKJ36 showed protein bands with anticipated sizes with respect to each putative gene product. S 1 endonuclease treatment and Southern hybridization suggested that pKJ36 replicates by a rolling circle mechanism via a single-stranded DNA (ssDNA) intermediate. A shuttle vector between E. coli and Bifidobacterium sp. was constructed using the pKJ36, pBR322, and staphylococcal chloramphenicol acetyl transferase (CAT) gene. The successful transformation of the Bifidobacterium strains was shown by Southern hybridization and PCR. The transformation efficiency differed from strain to strain and, depending on the electroporation conditions, with a range between $1.2{\times}10^1-2.6{\times}10^2{\;}cfu/\mu\textrm{g}$ DNA.X> DNA.

• Journal of the Korean earth science society
• /
• v.33 no.3
• /
• pp.242-258
• /
• 2012

The Yellow Sea has sensitively responded to high-amplitude sea-level fluctuations during the late Quaternary. The repeated inundation and exposure have produced distinct transgression-regression successions with extensive exposure surfaces in Kyeonggi Bay. The late Quaternary strata consist of four seismic stratigraphic units, considered as depositional sequences (DS-1, DS-2, DS-3, and DS-4). DS-1 was interpreted as ridge-forming sediments of tidal-flat and estuarine channel-fill facies, formed during the Holocene highstand. DS-2 consists of shallow-marine facies in offshore area, which was formed during the regression of Marine Isotope Stage (MIS)-3 period. DS-3 comprises the lower transgressive facies and the upper highstand tidal-flat facies in proximal ridges and forced regression facies in distal ridges and offshore area. The lowermost DS-4 rests on acoustic basement rocks, considered as the shallow-marine and shelf deposits formed before the MIS-6 lowstand. This study suggests six depositional stages. During the first stage-A, MIS-6 lowstand, the Yellow Sea shelf was subaerially exposed with intensive fluvial incision and weathering. The subsequent rapid and high amplitude rise of sea level in stage-B until the MIS-5e highstand produced transgressive deposits in the lowermost part of the MIS-5 sequence, and the successive regression during the MIS-5d to -5a and the MIS-4 lowstand formed the upperpart of the MIS-5 sequence in stage-C. During the stage-D, from the MIS-4 lowstand to MIS-3c highstand period, the transgressive MIS-3 sequence formed in a subtidal environment characterized by repetitive fluvial incision and channel-fill deposition in exposed area. The subsequent sea-level fall culminating the last glacial maximum (Stage-E) made shallow-marine regressive deposits of MIS-3 sequence in offshore distal area, whereas it formed fluvial channel-fills and floodplain deposits in the proximal area. After the last glacial maximum, the overall Yellow Sea shelf was inundated by the Holocene transgression and highstand (Stage-F), forming the Holocene transgressive shelf sands and tidal ridges.