• 한국환경성돌연변이발암원학회지
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• 제16권2호
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• pp.77-82
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• 1996

The RAD3 gene of Saccharomyces cerevisiae is required for excision repair and is essential for cell viability. RAD3 encoded protein possesses a single stranded DNA-dependent ATPase and DNA-RNA helicase activies. To examine the extent of conservation of structure and function of RAD3 during eukaryotic evolution, we have cloned the RAD3 homolog, HRD3, from the distantly related yeast Schizosaccharomyces pombe. Here, we report the partial cloning and characterization of HRD3 gene (Homologous of RAD3 gene) which was isolated by PCR amplification using conserved domain of Saccharomyces cerevisiae RAD3 gene. Chromosomal DNA isolated from S. pombe had similar restriction patterns to those from S. cerevisiae, as determined by Southern blot analysis. The 2. 8 kb transcript of mRNA was identified by Northern hybridization. The level of transcript did not increase upon UV-irradiation, suggesting that the HRD3 gene in S. pombe is not UV-inducible.

• 한국자기공명학회논문지
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• 제20권3호
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• pp.66-70
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• 2016

The replication protein A (RPA), is a heterotrimer with 70, 32 and 14 kDa subunits and plays a crucial role in DNA replication, recombination, and repair. The largest subunit, RPA70, binds to single-stranded DNA (ssDNA) and mediates interactions with many cellular and viral proteins. In this study, we performed nuclear magnetic resonance experiments on the complex of the DNA binding domain A of human RPA70 (RPA70A) with ssDNA, d(CCCCC), at various temperatures, to understand the temperature dependency of ssDNA binding affinity of RPA70A. Essential residues for ssDNA binding were conserved while less essential parts were changed with the temperature. Our results provide valuable insights into the molecular mechanism of the ssDNA binding of human RPA.

• Interdisciplinary Bio Central
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• 제2권3호
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• pp.8.1-8.5
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• 2010

Introduction: Recently identified human homologues of ALKB protein have shown the activity of DNA damaging drugs, used for cancer therapy. Bioinformatics study of hABH2 and hABH3 had led to the discovery of a novel DNA repair mechanism. Very little is known about structure and function of hABH4, one of the members of this superfamily. Therefore, in present study we are intended to predict its structure and function through various bioinformatics tools. Materials and Methods: Modeling was done with modeler 9v7 to predict the 3D structure of the hABH4 protein. This model was validated with the program Procheck using Ramachandran plot statistics and was submitted to PMDB with ID PM0076284. The 3d2GO server was used to predict the functions. Residues at protein ligand and protein RNA binding sites were predicted with 3dLigandSite and KYG programs respectively. Results and Discussion: 3-D model of hABH4, ALKBH4.B99990003.pdb was predicted and evaluated. Validation result showed that 96.4 % residues lies in favored and additional allowed region of Ramachandran plot. Ligand binding residues prediction showed four Ligand clusters, having 24 ligands in cluster 1. Importantly, conserved pattern of Glu196-X-Pro198- Xn-His254 in the functional domain was detected. DNA and RNA binding sites were also predicted in the model. Conclusion and Prospects: The predicted and validated model of human homologue hABH4 resulted from this study may unveil the mechanism of DNA damage repair in human and accelerate the research on designing of appropriate inhibitors aiding in chemotherapy and cancer related diseases.

• Asian Pacific Journal of Cancer Prevention
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• 제15권24호
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• pp.10917-10922
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• 2015

Background: This study was conducted to determine DEPDC1 expression in hepatocelluar carcinomas (HCCs) and to reveal its potential role in diagnosis and prognosis of affected patients. Materials and Methods: DEPDC1 expression at the mRNA level was detected by quantitative real-time PCR (qRT-PCR) in 205 cases of HCC and paired adjacent normal liver tissues, and by semi-quantitative RT-PCR in 20 cases. Survival curves were obtained by using Kaplan-Meier method and Log-rank test. Independent predictors associated with regard to disease free survival (DFS) and overall survival (OS) were identified using the Cox proportional hazard model. Results: High DEPDC1 mRNA levels were detected in 144 out of 205 cases (70.24%) of HCC, significantly associated with clinicopathological parameters, including tumor size (${\geq}4cm$), alpha-fetoprotein (${\geq}100ng/ml$), B-C of BCLC stage and recurrence. Kaplan-Meier survival analysis revealed that HCC patients with high DEPDC1 expression had poor OS and DFS. Multivariate analysis demonstrated that high DEPDC1 expression was an independent predictor for OS (HR=1.651; 95% 95%CI, 1.041-2.617; p=0.033) and DFS (HR=1.583; 95%CI, 1.01-2.483; p=0.045). Conclusions: Our results indicate DEPDC1 might be a novel diagnostic marker and an independent prognostic predictor for HCC patients.

• 한국건축시공학회지
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• 제24권3호
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• pp.355-362
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• 2024

본 연구의 주요 목표는 아파트 단지 수선유지 비용을 예측하기 위해 딥러닝 기법을 적용한 예측 모델 구축 프레임워크를 제안하는 것이다. 아파트 건물을 이상적인 상태로 관리하기 위해서는 지속적인 유지 및 시의적절한 수리가 필수적이다. 아파트 단지는 광범위한 면적, 공동 시설, 다수의 주거 동, 서비스 지역 등으로 인해 유지관리가 복잡하다. 또한, 아파트의 안전성 보장, 가치 유지 및 경제적 효율성 때문에 경제적이고 합리적인 유지보수의 중요성이 점점 커지고 있다. 그러나 아파트 단지 수선유지는 다양한 외부 요인의 영향을 받고 데이터 수집이 어려워 연구가 부족한 상황이다. 따라서 본 연구는 실제 아파트 단지 유지보수 비용 데이터를 기반으로 딥러닝 기법을 활용해 유지보수 비용을 예측하는 모델 개발 프레임워크를 제시하고자 한다. 본 연구의 프레임워크 및 결과는 실질적으로 아파트 단지의 유지보수 비용 예측에 활용될 수 있으며, 궁극적으로 아파트 단지의 시설 관리 향상에 기여할 것이다.

• 대한토목학회논문집
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• 제32권6A호
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• pp.411-418
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• 2012

이 연구에서는 수치해석 실험을 통하여, 원주방향 관통균열을 갖는 원통형 쉘의 패치보강 전후의 거동에 대한 평가와 다양한 변수에 따른 패치보강 효과를 분석하였다. 해석 모델의 신뢰성을 높이기 위해, h-법 및 p-법에 기초한 모델링, 두 가지 방법이 동시에 고려되었다. 또한 선형탄성파괴역학 개념에 기초하여 에너지 방출률을 산정하기 위해, 등가영역적분법 및 가상균열확장법이 고려되었다. 해석 예제로서, 먼저 연구에서 수행된 h-법 및 p-법 유한요소 모델을 검증하기 위해, 패치 보강전의 인장력을 받는 관통 균열이 있는 쉘 구조물이 해석되었으며, 해석 결과값들과 여러 참고문헌 값들이 비교되었다. 그리고 패치 보강된 원통형 쉘 시스템에서의 접착제 두께, 접착제 전단탄성계수, 패치 두께, 패치 재료, 균열 길이 등의 여러 설계 변수에 대한 민감도 해석이 수행되었다.

• 제어로봇시스템학회논문지
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• 제19권5호
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• pp.435-441
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• 2013

A swarm robot system consists of with multiple mobile robots, each of which is called an agent. Each agent interacts with others and cooperates for a given task and a given environment. For the swarm robotic system, the loss of the entire work capability by malfunction or damage to a single robot is relatively small and replacement and repair of the robot is less costly. So, it is suitable to perform more complex tasks. The essential component for a swarm robotic system is an inter-robot collaboration strategy for teamwork. Recently, the swarm intelligence theory is applied to robotic system domain as a new framework of collective robotic system design. In this paper, FA (Firefly Algorithm) which is based on firefly's reaction to the lights of other fireflies and their social behavior is employed to optimize the group behavior of multiple robots. The main application of the firefly algorithm is performed on path planning of swarm mobile robots and its effectiveness is verified by simulations under various conditions.

• BMB Reports
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• 제51권6호
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• pp.290-295
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• 2018

Y-box binding protein 1 (YB-1) is a member of the cold-shock domain (CSD) protein superfamily. It participates in a wide variety of cellular events, including transcription, RNA splicing, translation, DNA repair, drug resistance, and stress responses. We investigated putative functions of YB-1 in HIV-1 replication. Functional studies using overexpression or knockdown of YB-1 in conjunction with transfection of proviral DNA showed that YB-1 enhances virus production. We found YB-1 regulates HIV-1 production by stimulating viral transcription using HIV-1 LTR sequence U3RU5 with Luciferase assay. We also identified a specific region from amino acids 1 to 324 of YB-1 as necessary for the participation of the protein in the production of virions.

• 한국산학기술학회논문지
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• 제9권2호
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• pp.286-290
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• 2008

본 논문은 고체 추진 로켓의 연소 중에 발생하는 고체추진체의 동적 파괴 현상 및 유체-구조 상호작용을 시뮬레이션 하기 위한 프로그램 개발에 대한 것이다. 개발된 프로그램은 구조해석을 위한 CVFE (cohesive Volumetric Finite Element) 방법과 외재적 ALE (Arbitrary Lagrangian Eulerian) 방법을 응용한 유한요소법 코드와 유동해석을 위한 외재적 비정렬 유한 체적 오일러 코드(Explicit Unstructured Finite Volume Euler code)로 구성된다. 개발된 프로그램의 또 다른 중요한 특징은 균열의 전파와 고체추진체의 변형에 따라 생기는 추진제 형상의 대변형이 발생할 때, 새로 생긴 유체 영역에서의 격자의 확장과 복구되는 능력이다.

• Biomolecules & Therapeutics
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• 제20권2호
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• pp.133-143
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• 2012

Matrix metalloproteinases (MMPs) are a subfamily of zinc-dependent proteases that are re-sponsible for degradation and remodeling of extracellular matrix proteins. The activity of MMPs is tightly regulated at several levels including cleavage of prodomain, allosteric activation, com-partmentalization and complex formation with tissue inhibitor of metalloproteinases (TIMPs). In the central nervous system (CNS), MMPs play a wide variety of roles ranging from brain devel-opment, synaptic plasticity and repair after injury to the pathogenesis of various brain disorders. Following general discussion on the domain structure and the regulation of activity of MMPs, we emphasize their implication in various brain disorder conditions such as Alzheimer's disease, multiple sclerosis, ischemia/reperfusion and Parkinson's disease. We further highlight accumu-lating evidence that MMPs might be the culprit in Parkinson's disease (PD). Among them, MMP-3 appears to be involved in a range of pathogenesis processes in PD including neuroinflamma-tion, apoptosis and degradation of ${\alpha}$-synuclein and DJ-1. MMP inhibitors could represent poten-tial novel therapeutic strategies for treatments of neurodegenerative diseases.