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Short -term changes of microbial communities after control of Cochlodinium polykrikoides by yellow clay and chemical compound dosing in microcosm experiments (황토와 화학물질 살포에 의한 적조생물Cochlodinium polykrikoides 제어에 따른 미소생물그룹의 단주기변화)

  • Baek, Seung Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.4
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    • pp.2971-2977
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    • 2015
  • This study aimed to understand the changes in microbial community after algicide dosing to control the fish-killing dinoflagellate Cochlodinium polykrikoides in 10L microcosm. Based on our microcosm experiments, the algicidal activity for C. polykrikoides of yellow clay at the concentrations of 4g and 10g per 10 L was < 20%. At $0.8{\mu}M$ concentration of thiazolidinedione(TD49), the population of C. polykrikoides was controlled to be > 85%. In microbial community, a significant increase in heterotrophic bacterial (HB) abundance was observed at day 1 in the TD49 and yellow clay treatments including control. The HB remained high for 2 days and then gradually decreased. In contrast, the abundance of heterotrophic nanoflagellates (HNFs) increased significantly on days 3 and 5 in the TD49 treatments, indicating that the decline in HB was probably a result of predation by the high density of HNFs. In addition, fluctuations in the aloricate ciliate Uronema sp., which feed on bacteria, was clearly correlated with fluctuations in HB abundance, with a lag period of 1-3 days. Therefore, the short-term responses of the HNF and Uronema sp. may have been a result of the rapidly increasing of HB abundance, which is related to degradation of the dense C. polykrikoides bloom, particularly in the TD49 treatment. In addition, large aloricate ciliate Euplotes sp. was significantly increased after reproduction of HNFs and Uronema sp. Consequently, the algicide TD49 had positive effect on the microbial communities, which indicates that the microbial loop was temporarily enhanced in the microcosm by energy flow from HB through HNFs to ciliate.

Amelioration of metabolic disturbances and adipokine dysregulation by mugwort (Artemisia princeps P.) extract in high-fat diet-induced obese rats (쑥 (Artemisia princeps P.) 추출물이 고지방식이를 급여한 흰쥐의 대사장애 및 아디포카인 조절에 미치는 영향)

  • Kim, Yun-Hye;Park, Chung-Mu;Yoon, Gun-Ae
    • Journal of Nutrition and Health
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    • v.49 no.6
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    • pp.411-419
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    • 2016
  • Purpose: Dysregulation of adipokines caused by excess adipose tissue has been implicated in the development of obesity-related metabolic diseases. This study evaluated the effects of mugwort (Artemisia princeps Pampanini) ethanol extract on lipid metabolic changes, insulin resistance, adipokine balance, and body fat reduction in obese rats. Methods: Male Sprague-Dawley rats were fed either a control diet (NC), high-fat diet (HF, 40% kcal from fat), or high-fat diet with 1% mugwort extract (HFM) for 6 weeks. Results: Epididymal and retroperitoneal fat mass increased in the HF group compared with the NC group, and epididymal fat mass was reduced in the HFM group (p < 0.05). No difference was observed in serum levels of total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) among the groups. However, triglyceride (TG), TG/HDL-C ratio, and TC/HDL-C ratio increased in the HF group and significantly decreased in the HFM group. TG and TC levels in the liver were significantly higher in the HF group, whereas these levels were significantly reduced in the HFM group. HF rats had lower insulin sensitivity as indicated by increased homeostasis model assessment of the insulin resistance (HOMA-IR) value. HOMA-IR values significantly decreased in the HFM group. Adiponectin levels were higher in NC rats, and their leptin and PAI-1 levels were lower. Relative balance of adipokines was reversed in the HF group, with lower adiponectin levels but higher leptin and PAI-1 levels. In contrast, the HFM group maintained balance of adiponectin/leptin and adiponectin/PAI-1 levels similar to NC by reducing leptin and PAI-1 levels. Conclusion: Overall data indicated that mugwort extract can be effective in alleviating metabolic dislipidemia, insulin resistance, and adipokine dysregulation induced by a high-fat diet.

Structure and Dynamics of the Cold Water in the Western Channel of the Korea Strait (대한해협 서수도 냉수의 구조와 역학)

  • Cho, Yang-Ki;Kim, Kuh;Kim, Young-Gyu
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.9 no.3
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    • pp.132-139
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    • 1997
  • CTD and current observation were taken to investigate the structure of the cold water in the Western Channel of the Korea Strait in October 1993. Thickness of the cold water in the deep trough of the strait changes from 20 m to 70 m according to the water depth. Thermocline between the Tsushima Warm Water and the cold water deepens from north to south with 0.00057 in slope. Temporal variation of the thickness appears to be related with the tidal current. The maximum variation is 20 m for 48 hours. Mean velocity of the cold water for 72 hours is 17 cm/sec southward. A simple model was used to understand dynamically the southward flow of the cold water and the return flow at the upper part in the lower layer. Calculated maximum southward flow and eddy viscosity coefficient are 7 cm/sec and 0.038 $m^2$/sec respectively in the model. Southward transport is $0.032$\times$10^6㎥/sec$ at the northern part in the trough and decreases from north to south due to the presence of the return flow. Southward transport increases with the increase in the upper layer transport but is not affected by the density of the upper layer or the interface slope.

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Analysis of Water Retention Capacity at Sasa borealis Stands in Jirisan National Park (지리산국립공원 내 조릿대 임분의 수원함양기능 분석)

  • Ji, Hyung Woo;Park, Jae Hyeon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.11 no.3
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    • pp.1-11
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    • 2008
  • Although landslides were frequently occurred under Tripterygium regelii and Rubus sp. vegetations, the damage of landslide was not observed in sasa (Sasa borealis) stands. These phenomena may be affected by forest vegetation types. This result suggested that the landslide occurred in Jirisan (Mt.) National Park may be closely related to water retention capacity at Sasa borealis stands. This study compared and analyzed the water retention capacity of each soil horizon of sasa, larch (Larix leptolepis) and mongolian oak (Quercus mongorica) stands. Soil bulk density in A horizon was lower in sasa (0.776g/$cm^3$) than in mongolian oak (0.828g/$cm^3$) and in larch stands (1.282g/$cm^3$). Water permeability in A horizon was 0.02055cm/sec for sasa, 0.00575cm/sec for mongolian oak, and 0.0007cm/sec for larch stands, respectively. The water permeability of sasa stand was about 3.6 times and about 29 times higher than in mongolian oak and in larch stands, respectively. This result indicates that water infiltration of soil surface during a rain event is more rapid in sasa than in other two stands. Soil organic matter content in B horizon was lower in larch (0.7%) than in mongolian oak (6.5%) and in Sasa (3.3%) stands. The solid ratio in A horizon was highest in larch among three stands, but that of mongolian oak and larch stands showed a similar rate. Pore space rates was 70.7% for A horizon and 70.6% for B horizon of sasa, 68.9% for A horizon and 70.6% for B horizon of sasa, 68.9% for A horizon and 70.6% for B horizon of mongolian oak forests and 51.7% for A horizon and 49.2% for B horizon of larch forests, respectively. According to pore space rates, the water retention capacity may be poor in larch stand compared with other two stands. Soil strength in sasa and mongolian stands was over 25kgf/$cm^2$ from 40cm depth, while the strength was over 25kgf/$cm^2$ from 25cm depth in larch stand. The result indicates that tree growth and water permeability in larch stand could be limited due to high soil strength. Larch stand was poor for soil pore space development to be offered to the water retention capacity, but water retention capacity of A horizon soil in sasa stand was high than that of other two stands. Therefore, establishment of sasa stand under larch stand could help to prevent landslides.

Thermal and Electrical Behaviors of Polyethylene Oxide/Polyaniline Fibers Prepared by Electrospinning Method (전기방사법에 의해 제조된 폴리에틸렌옥사이드/폴리아닐린 섬유의 열적 및 전기적 거동)

  • Kim, Seok;Cho, Mi-Hwa;Park, Soo-Jin
    • Applied Chemistry for Engineering
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    • v.17 no.1
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    • pp.16-21
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    • 2006
  • In this study, PEO blend fibers mixed with polyaniline (PANI)/10-camphor sulfonic acid (CSA) and PANI/dodecylbenzene sulfonic acid (DBSA) were electro spun to investigate the influence of PANI content. CSA and DBSA were used as a functionalized doping acid having a bulky volume. PANI/PEO blend solution was prepared by dissolving PEO and PANI doped with CSA or DBSA. The thermal properties were measured by thermogravimetric analyzer (TGA). As a result, with increasing of the PANI content in PANI/CSA and PANI/DBSA, although initial decomposition temperature (IDT) was decreased, thermal stability was increased due to the increase of $A^*{\cdot}K^*$ and integral procedural decomposition temperature (IPDT). The electrical conductivities measured by the 4-probe method. The electric conductivity was increased with increasing of PANI content in PANI/CSA and PANI/DBSA. However, electrical conductivity did not change significantly beyond 30% content of PANI. From CV results, PANI/CSA showed the better defined peak shpae and higher peak current density compared to PANI/DBSA. This was probably related to the slightly higher electrical conductivity or better morphology for easy charge transfer in the case of PANI/CSA.

Shading Effects on Growth and Chlorophyll Contents of Abies holophylla (인공 피음처리에 따른 전나무의 생장과 엽록소 함량변화에 관한 연구)

  • Woo Su-Young;Lee Dong-Sup;Kwon Oh-Kyu
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.1 no.2
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    • pp.97-102
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    • 1999
  • This study was conducted to compare seasonal changes of height, diameter at root collar, biomass and chlorophyll contents of Abies holophylla seedlings grown in Sangju National University Nursery under different light intensities. Four light intensities were adopted using nylon net such as 100% sunlight(1800 $\mu$㏖ m$^{-2}$ s$^{-1}$ ), 75% of sunlight (1350 $\mu$㏖ m$^{-2}$ s$^{-1}$ ), 50% of sunlight (900 $\mu$㏖ m$^{-2}$ s$^{-1}$ ) and 25% of sunlight (450 $\mu$㏖ m$^{-2}$ s$^{-1}$ ). Planting density in four light intensities was 30 cm$\times$30 cm. In each treatment, 100 trees were planted and height, diameter at root collar, biomass and chlorophyll contents were measured. The highest height, diameter at root collar and biomass in Abies holophylla seedlings were observed at 100% full sunlight. Lowest chlorophyll contents (Chlorophyll a, b and total) were shown at 25% of sunlight, lowest light intensity treatment in this study. Growths and chlorophyll contents of Abies holophylla seedlings were strongly associated with light intensity. Photosynthesis has to be investigated in near future because photosynthesis and chlorophyll contents were strongly related to tree growth with long periods.

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The Effects of Treadmill Training on Neurotrophins and Immediately Early Protein in Obese Rats (트레드밀 트레이닝이 비만 쥐의 neurotrophins와 초기발현 단백질에 미치는 영향)

  • Woo, Jin-Hee;Shin, Ki-Ok;Yeo, Nam-Heoh;Park, So-Young;Kang, Sung-Hwun
    • Journal of Life Science
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    • v.21 no.7
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    • pp.985-991
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    • 2011
  • The purpose of this study was to investigate the biological effect of obesity-induced oxidative damage on neurogenesis and early protein expression. Obesity was induced I thirty 4-week old male Sprague-Dawley rats through a high fat diet for 15 weeks. After one week of environmental adaptation, the rats were divided into 2 groups: high fat diet sedentary group (HDS, n=15) and high fat diet training group (HDT, n=15). Exercise training was performed 5 times a week for 8 weeks, with mild-intensity treadmill running for weeks 1-4 and moderate-intensity treadmill running for weeks 5-8. After the 8 week training period, we analyzed lipid profiles, serum 8-hydroxyguanosine (8-OHdG), liver tissue malondialdehyde (MDA) related to oxidative damage factors, nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), c-fos, c-jun, and extracellular signal regulated kinase (Erk) in the hippocampus. The results of this study are as follows. There were differences between HDS and HDT in triglyceride (TG) and total cholesterol (TC) (p<0.05). In high density lipoprotein (HDL-c), the HDT was higher than HDS after treadmill training (p<0.05). In 8-OHdG, the HDT was lower than HDS after treadmill training (p<0.05). Genetic expressions of c-jun, BDNF and MDA in the HDT were higher than in the HDS after treadmill training in hippocampus (p<0.05). Therefore, we conclude that 8 weeks of treadmill training can improve imbalanced lipid profiles, reduce oxidative damage, and activate neurogenesis in obese rats.

Analysis of Temporal and Spatial Distribution of Traffic Accidents in Jinju (진주시 교통사고의 시계열적 공간분포특성 분석)

  • Sung, Byeong Jun;Bae, Gyu Han;Yoo, Hwan Hee
    • Journal of Korean Society for Geospatial Information Science
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    • v.23 no.2
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    • pp.3-9
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    • 2015
  • Since changes in land use in urban space cause traffic volume and it is closely related to traffic accidents. Therefore, an analysis on the causes of traffic accidents is judged to be an essential factor to establish the measure to reduce traffic accidents. In this regard, the analysis was conducted on the clustering by using the nearest neighbor indexes with regard to the occurrence frequencies of commercial and residential zone based on traffic accident data of the past five years (2009-2013) with the target of local small-medium sized city, Jinju-si. The analysis results, obtained in this study, are as follows: the occurrence frequency of traffic accidents was the highest in spring and the lowest in winter respectively. The clustering of traffic accident occurrence at nighttime was stronger than at daytime. In addition, terms of the analysis on the clustering of traffic accident according to land use, changes according to the seasons was not significant in commercial areas, while clustering density in winter tended to become significantly lower in residential areas. The analysis results of traffic accident types showed that the side-right angle collision of cars was the highest in frequency occurrence, and widespread in both commercial areas and residential areas. These results can provide us with important information to identify the occurrence pattern of traffic accidents in the structure of urban space, and it is expected that they will be appropriately utilized to establish measures to reduce traffic accidents.

Anti-obesity effects of cultivated ginseng, -wild simulated ginseng and -red ginseng extracts (인삼, 산양산삼 및 홍삼 추출물의 항비만 효과)

  • Kim, Ha-Rim;Lee, Chang-Hyun;Jung, Mun Yhung;Kim, Jong-Seok;Kim, Hong-Jun;Jeon, Hye-Sook;Lee, Seung-Hyun;Kim, Ji-Hyun;Shin, Min-Ji;Ma, Sang-Yong;Kwon, Jin;Oh, Chan-Ho
    • Herbal Formula Science
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    • v.27 no.4
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    • pp.269-284
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    • 2019
  • This study was to investigate whether cultivated ginseng (CG), cultivated wild simulated ginseng (CWG) and cultivated red ginseng (CRG) extracts influences on the obesity. The saponin contents of 3 kinds of ginsengs were analysed by HPLC-ESI-TOF-MS. Total saponin contents were determined in CG on the most contents and since red ginseng has the highest PD (protopanaxadiol type) / PT (protopanaxatriol type) ratio, there may be differences between ginseng, wild ginseng, and red ginseng with respect to their pharmacological effects. Male C57BL/6J mice were fed a normal diet(N), HFD (60% Kcal fat, C), HFD with CG, CWG and CRG extracts (800 mg/kg) for 5 weeks. We observed change of total body weight, degree of hepatic lipid accumulation and immunohistochemical change of GLP-1 and insulin-secreting cells. Also this study attempts to use the physiological analysis method to analyze the changes of blood lipids, insulin and leptin concentration. The change of body weight and size of accumulated lipid droplets in liver lobules decreased in all of the experimental groups than the control(C) group. In the pancreas, the immunohistochemical density of insulin-secreting cells were significantly stronger in the CWG and CRG than C group. The levels of serum insulin and leptin significantly decreased 55.6%, 54.3% respectively in CWG and CRG. The changes of triglyceride, total cholesterol in serum decreased in CRG than the C group. Obesity related CG, CWG and CRG extracts might have contribute to improvement of obesity by regulating the levels of blood lipids and biochemical indicator of fat accumulation.

Analysis on Adhesion Properties of Composite Electrodes for Lithium Secondary Batteries using SAICAS (SAICAS를 이용한 리튬이차전지용 복합전극 결착특성 분석)

  • Byun, Seoungwoo;Roh, Youngjoon;Jin, Dahee;Ryou, Myung-Hyun;Lee, Yong Min
    • Journal of the Korean Electrochemical Society
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    • v.21 no.2
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    • pp.28-38
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    • 2018
  • Although the adhesion properties of composite electrodes are important for securing long-term reliability and realizing high energy density of lithium secondary batteries, related research has not been carried out extensively due to the limitation of measurement technology. However, surface and interfacial cutting analysis system(SAICAS), which can measure the adhesion properties while cutting and peeling a coating layer of $1{\sim}1000{\mu}m$ thickness, has been developed and applied for analyzing the adhesion properties of composite electrodes for lithium secondary batteries. Thus, this review presents not only the principle and measurement method of SAICAS but also comparison results between SAICAS and conventional peel test. In addition, application examples of SAICAS are introduced in the study of electrode design optimization, new binder derivation study, and binder distribution in composite electrode. This suggests that SAICAS is an analytical method that can be easily applied to investigate the adhesion properties of composite electrodes for lithium secondary batteries.