• Proceedings of the Ginseng society Conference
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• 2002.10a
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• pp.96-112
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• 2002

In the present study, we have investigated the effects of centrally administered ginsenoside Rc or Rgl on the modulation of NMDA receptor and $GABA_A$ receptor binding in rat brain. The NMDA receptor binding was analyzed by quantitative autoradiography using $[^3H]MK-801$ binding, and $GABA_A$ receptor bindings were analyzed by using $[^3H]muscimol\;and\;[^3H]flunitrazepam$ in rat brain slices. Rats were infused with ginsenoside Rc or Rg1 ($10\;{\mu}g/10{\mu}l/hr$, i.c.v.) for 7 days, through pre-implanted cannula by osmotic minipumps (Alzet, model 2ML), The levels of $[^3H]MK-801$ binding were highly decreased in part of cortex and cingulated by ginsenoside Rc and Rgl. The levels of $[^3H]muscimol$ binding were strongly elevated in almost all regions of frontal cortex by the treatment of ginseoside Rc but decreased by ginsenoside Rg 1. However, the $[^3H]flunitrazepam$ binding was not modulated by ginsenoside Rc or ginsenoside Rgl infusion. These results suggest that prolonged infusion of ginsenoside could differentially modulate $[^3H]MK-801\;and\;[^3H]muscimol$ binding in a region-specific manner. Also, we investigated the influence of centrally administered ginsenoside on the regulation of mRNA levels of the family of NMDA receptor subtypes (NR1, NR2A, NR2B, NR2C) by in situ hybridization histochemistry in the rat brain. The level of NR1 mRNA is significantly increased in temporal cortex, caudate putamen, hippocampus, and granule layer of cerebellum in Rgl-infused rats as compared to control group. The level of NR2A mRNA is elevated in the frontal cortex. In contrast, it was decreased in CAI area of hippocampus in Rgl-infused rats. However, there was no significant change of NR1 and NR2A mRNA levels in Rc-infused rats. The level of NR2B mRNA is elevated in cortex, caudate putamen, and thalamus in both Rc- and Rg-infused rats. In contrast, NR2B level is decreased in CA3 in Rgl-infused rats. The level of NR2C mRNA is increased in the granule layer of cerebellum in only Rg1 but not Rc infused rats. These results show that structure difference of ginsenoside may diversely affect the modulation of expression of NMDA receptor subunit mRNA after infusion into cerebroventricle in rats.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.3
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• pp.476-482
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• 2019

Regulatory protocols such as the Convention on Climate Change and the regulation of greenhouse gas emissions act as catalysts for the development of high-efficiency energy equipment and the efficient use of energy. Among the fields where energy consumption is high, the electric heating equipment is not efficient. The electric boiler mainly uses a method of circulating water by contacting the heater. When the existing electric boiler is used, the water minerals are contacted with the high-temperature heater to be carbonized and adsorbed, thereby promoting the corrosion of the heater and lowering the efficiency of the heater. For this reason, an electric induction boiler, which has high energy efficiency and is applied to an induction heating system that can uniformly heat the object to be heated rather than conduction or convection heating, is in the limelight. This method induces a boiler pipe And it is recognized as an alternative that can solve the problem that occurs when heating is performed by direct heating. Despite the fact that research on induction heating has been conducted for a relatively long period of time, there have been few studies on the electrothermal technology using induction heating. Therefore, in this paper, to improve the heat efficiency of electric induction boiler, the influence of the cross sectional area, number of windings and winding layers is analyzed by finite element method through parametric study method. The method of finding the design point which maximizes the total loss is proposed by the alternating winding design method which can maximize the heat generation by analyzing copper and iron losses.

• Journal of Life Science
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• v.29 no.10
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• pp.1055-1061
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• 2019

Sarracenia purpurea as a carnivorous plant in the family Sarraceniaceae is known to require strong light for its growth and to absorb nutrients from the decomposed molecules of insects that are attracted by color, sweet juice, and the like. S. purpurea grew greenish in whole body under weak light conditions, while the whole of the insectivorous sac including leaves, is changed to dark red under strong light conditions. The phenomenon of reddish S. purpurea is thought to be related to the flavonoid pigment anthocyanin. Interestingly, the color change was not observed when S. purpurea was grown in a growth condition with abundant nitrogen fertilizer. The expression levels of anthocyanin contents and biosynthesis-related genes were strongly correlated with light intensity and nitrogen fertilizer. The anthocyanin content in the strong light condition ($240{\mu}mol\;m^{-2}s^{-1}$) was 6.15 times higher than that in the weak light ($40{\mu}mol\;m^{-2}s^{-1}$). In contrast, the anthocyanin contents were not significantly changed when 0.8% urea solution was supplied as nitrogen fertilizer. Consistently, CHALCONE SYNTHASE (CHS) gene was up-regulated by strong light and down-regulated by nitrogen fertilizer. These results suggest that the environmental changes of light and nitrogen in soil regulate the anthocyanin content in S. purpurea.

• The Journal of the Convergence on Culture Technology
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• v.7 no.3
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• pp.553-564
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• 2021

This study analyzed the the risk of halogen lamp for pigsty heating and its fire prevention. Halogen lamps are used for keeping warm in pen because of their excellent economy and convenience. However, there is a high risk of fire due to poor safety management and exposure to animal movements. In fact, after exploring old pigsty and cattle shed, it was easy to confirm that they were exposed to fire risks. We noted this point and studied the possibility of fire by experiment due to combustible contact with halogen lamp and carelessness in use. The experiment was conducted under conditions similar to the actual use environment. After installing halogen lamps to the height used in actual pen, the temperature change was observed with infrared camera with straw, sawdust and rice husks on the floor. And assuming abnormal conditions, the experiment was also conducted under conditions the lamp dropped on the floor or combustible material came into contact with the glass tube inside the lamp. If halogen lamps were used in normal condition, there was no risk of fire. However, in abnormal use environments, smoke comes out or even ignited. Even if it is convenient for use or highly utilized, high risk of fire will require setting up fire prevention measures or regulation of use. Through in-depth fire investigation and research, we should promote the risk of fire and make efforts to prevent fire to minimize human life and property damage.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.29 no.2
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• pp.108-117
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• 2011

In drafting the standards on changes in current conditions, the height or the number of stories is applied in a restrictive manner by limiting to securing the distance to vertical surface for cultural properties within the influence investigation area, but this is expected to have a negative impact on the surrounding sceneries as well as results in the dwarfing phenomenon for precious cultural properties. That is, the preparation for supplementing the insufficient objectivity that is likely to take place during the process of drafting the standards on changes in current conditions. Thus the author attempts to suggest the analytic method for the decision making related to objective and reasonable determination and regulation of the changes in current conditions through computer based simulation work that considers the cultural properties and surrounding environments under investigation. In order to achieve such research objectives, the author reviewed the subject sites where the cultural property dwarfing phenomenon was expected to occur in case of the permission for the changes in current conditions or where the impact of natural landscape and natural feature on the earth is less than architectural building or artificial structure or where the new policy program is likely to be adopted due to incomplete establishment of current condition change standard within influence investigation area, among other cultural properties with architectural building or artificial structure nearby located in Cheonan city and then selected Cheonansaji Dangganjiju(flag poles) and Jiksanhyun Gwana(government office). The author then undertook the quantitative visibility analysis in order to determine the comprehensive prospect rights for the cultural properties and surrounding environments concerned.

• Korean Journal of Labor Studies
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• v.17 no.1
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• pp.297-323
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• 2011

Part-time jobs in Sweden are highly feminized yet are in fair conditions in terms of job security, earnings, and collective representation. Three points are considered to be important to understand why part-time work in Sweden carries such positive characteristics. First, the part-time work in Sweden is widely spread not as a result of employers' need for labor flexibilization but as means to enhance the work-life balance, a value pursued within a broader social policy package to change the breadwinner model. Second, discrimination against part-time workers is restrained in Sweden because the boundary between part-time and full-time is not conspicuous. Most of part-time jobs are occupied by regular workers who exert the right to part-time work, hence may go back to the full-time status any time. Third, the regulation on overtime work of part-time workers as well as full-time workers is strong. It is largely agreed among researchers that part-time work contributed greatly to an increase of female employment rate in Sweden. Since the 1970s, the increased availability of part-time jobs induced married women who used to be economically inactive to the labor market and maintained them to be economically active throughout the child rearing period. From the gender perspective, one may still raise issues regarding part-time work in Sweden such as persistent feminization and strong occupational sex segregation. However, the observed trend shows that the part-time work in Sweden has functioned more as a stepping stone to the full-time work for women than as a women's trap.

• Korean Journal of Ecology and Environment
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• v.53 no.4
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• pp.324-330
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• 2020

Ozone (O₃) is a general disinfectant to remove micro-pollutants in water treatment system. Previous studies have reported effect of ozone to bacteria and pathogens removal, but its effect to the relatively large organisms has little known. In this study, we investigated potential effects of ozone toxicity to the non-bite midge larvae (Glyptotendipes tokunagai) with accumulate mortality, coloration change and expression of heat shock protein 70 (HSP70). The accumulate mortality rate of G. tokunagai increased in a dose-time dependent manner and the highest mortality rate was observed to 75% at 30 minute of exposure duration with 2.0 ppm of ozone concentration. Exposure to ozone was a factor increasing body color of the larvae. The tendency of HSP70 mRNA expression showed up-regulation in ozone exposure at 20 minute. After that time, the expression of HSP70 in exposed group decreased to a similar level of control group. Our results clearly showed that ozone toxicity affects physical and molecular activity of G. tokunagai, implying the potential hazardous of ozone in the aquatic ecosystem including macroinvertebrates.

• The Journal of the Korea Contents Association
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• v.21 no.3
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• pp.720-729
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• 2021

The purpose of this study was to measure repeated the process of non-suicidal self-injury individuals change in everyday life through the Ecological Momentary Assessment(EMA) daily diary. The study subjects of were 17 adults aged 19 to 29, accessed a link sent by text message on a cell phone and recorded a diary of the day's affects, interpersonal conflicts, self-injury thoughts, and behaviors once a day for two weeks. Using a total of 238 reported entries, the contextual factors of NSSI were examined, and the effects of affects and interpersonal conflicts on NSSI analyzed through a multi-level model. As a result, the negative affects of that day have a significant relation with within subject NSSI behavior and positive affects have a significant relation between subject NSSI behavior. These findings means that overall individuals with low positive affects have a higher risk of self-injury behavior compared to those with higher levles of positive emotions, and an increase in negative emotions on that day within an individual increases the risk of self-injury behavior on that day. In other words, it implies that it is important to manage negative emotions and strengthen overall positive affects for that day in the intervention of emotion-regulation of experienced self-injury individuals. It is meaningful that this study explored NSSI risk factors experienced in daily life through the short-term longitudinal study.

• Journal of Practical Agriculture & Fisheries Research
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• v.23 no.1
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• pp.117-128
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• 2021

The skeletal muscle development of Hanwoo steer has been processed in the prenatal and postnatal periods. Bovine satellite cell located in perimysium of muscle tissues has differentially distributed in peripheral tissues. The study of postnatal development of satellite cells can help understand the genetic and functional regulation of meat characteristics. Factors affecting muscle size increase are related to the accumulation of DNA or synthesis of RNA proteins. In this study, we observed muscle development and differentiation after culturing bovine satellite cells derived from longissimus dorsi and semimembranosus regions of Hanwoo muscle tissue. In addition, RNA sequencing data were analyzed for differentially expressed genes (DEG) involved in intracellular muscle development and growth. The DEG of the two muscle tissues were compared according to 1day, 2day, 4day, and 7day. The overall gene expression level was confirmed by the heat map. Gene Ontology (GO) classification method was used to compare the expression level of gene groups affecting LD and SM development. The histology of GO was consistent with the time-cause change of LD and SM cell morphology. SM showed more active skeletal muscle development than LD. Even within the same time, SM expressed more genes than LD, thus synthesizing more muscle fibers

• BMB Reports
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• v.55 no.2
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• pp.81-86
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• 2022

Macrophages are a major cellular component of innate immunity and are mainly known to have phagocytic activity. In the tumor microenvironment (TME), they can be differentiated into tumor-associated macrophages (TAMs). As the most abundant immune cells in the TME, TAMs promote tumor progression by enhancing angiogenesis, suppressing T cells and increasing immunosuppressive cytokine production. N-myc downstream-regulated gene 2 (NDRG2) is a tumor suppressor gene, whose expression is down-regulated in various cancers. However, the effect of NDRG2 on the differentiation of macrophages into TAMs in breast cancer remains elusive. In this study, we investigated the effect of NDRG2 expression in breast cancer cells on the differentiation of macrophages into TAMs. Compared to tumor cell-conditioned medium (TCCM) from 4T1-mock cells, TCCM from NDRG2-over-expressing 4T1 mouse breast cancer cells did not significantly change the morphology of RAW 264.7 cells. However, TCCM from 4T1-NDRG2 cells reduced the mRNA levels of TAM-related genes, including MR1, IL-10, ARG1 and iNOS, in RAW 264.7 cells. In addition, TCCM from 4T1-NDRG2 cells reduced the expression of TAM-related surface markers, such as CD206, in peritoneal macrophages (PEM). The mRNA expression of TAM-related genes, including IL-10, YM1, FIZZ1, MR1, ARG1 and iNOS, was also downregulated by TCCM from 4T1-NDRG2 cells. Remarkably, TCCM from 4T1-NDRG2 cells reduced the expression of PD-L1 and Fra-1 as well as the production of GM-CSF, IL-10 and ROS, leading to the attenuation of T cell-inhibitory activity of PEM. These data showed that compared with TCCM from 4T1-mock cells, TCCM from 4T1-NDRG2 cells suppressed the TAM differentiation and activation. Collectively, these results suggest that NDRG2 expression in breast cancer may reduce the differentiation of macrophages into TAMs in the TME.