• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2003.10a
• /
• pp.103-103
• /
• 2003

The purpose of this study is to evaluate an efficacy of in vitro differentiated human embryonic stem (hES, MB03) cells expressing Nurr1 in relief of symptomatic motor behavior of Parkinson's disease (PD) animal models MB03 was genetically modified to express Nurr1 protein and was induced to differentiate according to 2-/4+ protocol using retinoic acid and ascorbic acid. The differentiation-induced cells were selected for 10 to 20 days thereafter in N2 medium. Upon selection, cells expressing GFAP, TH, or NF200 were 38.8%, 11%, and 20.5%, respectively. in order to examine therapeutic effects of the differentiated cells in PD animal model, rats were unilaterally lesioned by administration of 6-kydroxydopamine HCI (6-OHDA) into medial forebrain region (MFB, AP -4.4 mm, ML 1.2 mm, DV 78 mm with incision bar set at -2.4 mm), as a reference to bregma and the surface of the skull. Confirmation of successful lesion by apomorphine-induced rotational behavior, differentiated cells were transplanted into the striatum (AP 1.0, ML 3.5, DV -5.0; AP 0.6, ML 2.5, DV -4.5). Improvements of asymmetric motor behavior by the transplantation were examined every two weeks after the surgery. In two weeks, numbers of rotation by the experimental rats were $-14.8 \pm 33.9%$ (P<0.05) of the number before transplantation, however, the ratio increased slightly to $13.6 \pm 56.3%$ in six weeks. In contrast, the ratio of sham-grafted animals ranged from 112.3+8.5% to 139.2+28.9% during the examination. Immunohistochemical studies further confirmed the presence, survival, migration, and expression of TH of the transplanted human cells.

• Journal of radiological science and technology
• /
• v.16 no.1
• /
• pp.57-65
• /
• 1993

Asymetric system have been introduced in these years by KODAK company nam of Insight system for the purpose of improve the chest image. We have had a problem of chest radiology that it is very difficult to visualize the lung field and modiastinal region at one shot. That's why we are the RT using the technique of high voltage hard quality radiography in chest radiography. Also it is known the c-type wide latitude film can lift up the density of mediastinal structures. Authors investigated the photographic characteristics and physical structure of Insight system. Method 1. Investigated the structure of Emulsion layer. Calculated the particle size of Insight system using SEM(Scanning Electron Microscope). 2. Photographic characteristics has been compared the Insight system with the ortho KM/MG combination in $60{\sim}120kV$ range. Results 1. The particle size of backside film were investigated about 2 times larger that of front side film. 2. The front and backscreen's thickness ratio was detected 1 : 3.87, that the backscreen's thickness was thicker than frontscreen. 3. At the view point of photographic characteristics the frontside of insight system make up the contrast, backside make up the density at low exposure lesion.

• 한국생물공학회:학술대회논문집
• /
• 2003.04a
• /
• pp.74-78
• /
• 2003

Multi-channel images of 11-MUA and 11-MUOH self-assembled monolayers were obtained by using two-dimensional surface plasmon resonance (SPR) absorption. Patterning process was simplified by exploiting direct photo-oxidation of thiol bonding (photolysis) instead of conventional photolithography. Sharper images were resolved by using a white light source in combination with a narrow bandpass filter in the visible region, minimizing the diffraction patterns on the images. The line profile calibration of the image contrast caused by different resonance conditions at each points on the sensor surface (at a fixed incident angle) enables us to discriminate the monolayer thickness in sub-nanometer scale. Furthermore, there is no signal degradation such as photo bleaching or quenching which are common in the detection methods based on the fluorescence.

• Molecules and Cells
• /
• v.41 no.4
• /
• pp.362-372
• /
• 2018

High mobility group box 2 (HMGB2) is an abundant, chromatin-associated, non-histone protein involved in transcription, chromatin remodeling, and recombination. Recently, the HMGB2 gene was found to be significantly downregulated during senescence and shown to regulate the expression of senescent-associated secretory proteins. Here, we demonstrate that HMGB2 transcription is repressed by p21 during radiation-induced senescence through the ATM-p53-p21 DNA damage signaling cascade. The loss of p21 abolished the downregulation of HMGB2 caused by ionizing radiation, and the conditional induction of p21 was sufficient to repress the transcription of HMGB2. We also showed that the p21 protein binds to the HMGB2 promoter region, leading to sequestration of RNA polymerase and transcription factors E2F1, Sp1, and p300. In contrast, NF-Y, a CCAAT box-binding protein complex, is required for the expression of HMGB2, but NF-Y binding to the HMGB2 promoter was unaffected by either radiation or p21 induction. A proximity ligation assay results confirmed that the chromosome binding of E2F1 and Sp1 was inhibited by p21 induction. As HMGB2 have been shown to regulate premature senescence by IR, targeting the p21-mediated repression of HMGB2 could be a strategy to overcome the detrimental effects of radiation-induced senescence.

• Archives of Plastic Surgery
• /
• v.43 no.1
• /
• pp.59-65
• /
• 2016

Background In East Asia, the foot is the most common site of cutaneous melanoma. The purpose of this study was to investigate the differences between cutaneous melanoma of the foot and melanomas of other sites. Methods We studied 52 patients who underwent surgical treatment for cutaneous melanoma of the foot from September 2000 to January 2015. Through a retrospective review of their medical records, we collected data relating to their sex, age, histopathological subtype, Clark level, tumor thickness, lymph node involvement, stage, and survival rate, and we compared these parameters to those of 61 patients treated for melanoma of other sites. Moreover, we analyzed the surgical procedures, complications, and mortality rates associated with cutaneous melanoma on various parts of the foot. Results Melanoma of the foot differed from other melanomas only in the histopathological subtype. Other clinical features, including survival rate, did not differ significantly between the two groups heel was the most common site of melanoma of the foot (22 cases, 42.3%). The method of reconstruction varied depending on the region involved. Conclusions A comparison of melanoma of the foot to other melanomas showed that melanoma of the foot was associated with a significantly different distribution of histological subtypes. Consistent with previous findings, we found that the acral lentiginous subtype was the most common among melanomas of the foot. Furthermore, in contrast with previous studies, we did not find a worse prognosis or lower survival rate for melanoma of the foot in comparison with other melanomas.

• Journal of Veterinary Clinics
• /
• v.23 no.3
• /
• pp.355-360
• /
• 2006

Four dogs with similar respiratory signs were referred to Veterinary Medical Teaching Hospital, Seoul National University. The clinical signs observed in these cases were anorexia, nasal discharge, sneezing, epistaxis, ocular discharge, and exophthalmoses. The routine laboratory tests revealed leukocytosis in two cases. On the skull radiographs, soft tissue density filled nasal cavity with loss of turbinate detail and increased density in frontal sinuses were found in all cases. Lysis of nasal bone was seen in two cases. Lysis of zygomatic arch was seen in one case. On computed tomography scan images, asymmetrical destruction of turbinate and nasal septum, and the superimposition of a soft tissue mass over the turbinate with peripheral contrast enhanced effect were identified in all cases. Destruction of ipsilateral orbital bone and invasion to retrobulbar region were visualized in all cases. In addition, all cases had lysis of cribriform plate. Lysis of nasal bone and destruction of hard palate were seen in two cases. Swelling of submandibular lymph node and salivary gland was seen in a case. Invasion to brain was identified in a case. All cases were diagnosed as nasal adenocarcinoma by cytology with fine needle aspiration and curettage.

• Journal of Veterinary Clinics
• /
• v.26 no.4
• /
• pp.331-335
• /
• 2009

This paper was performed to investigate the propensity of the diameter of maximum distended urethra from urethra to os penis in mature male dogs of 25 male dogs of different breeds. The measured sites of urethras were divided into 7 regions, i.e. prostate, membrane, isthmus, perineum, scrotum, prescrotum and os penis. By using the inflated balloon catheter filled with contrast medium, the maximum diameter of the distended urethras of each region was recorded and compared among regions. The mean diameter of the lumen from the prostatic urethra to the os penis urethra was gradually narrowed except for the isthmus portion, with a sense of resistance for retraction being noted at the level of ischiatic arch in 22 dogs. Proposed results from this should be utilized as a predictor of a treatment plan for the removal of urethroliths using an urohydropropulsion.

• Atmosphere
• /
• v.17 no.1
• /
• pp.55-68
• /
• 2007

Future climate changes over East Asia are projected by anthropogenic forcing of greenhouse gases and aerosols using ECHO-G/S (ECHAM4/HOPE-G). Climate simulation in the 21st century is conducted with three standard SRES scenarios (A1B, B1, and A2) and the model performance is assessed by the 20th Century (20C3M) experiment. From the present climate simulation (20C3M), the model reproduced reliable climate state in the most fields, however, cold bias in temperature and dry bias of summer in precipitation occurred. The intercomparison among models using Taylor diagram indicates that ECHO-G/S exhibits smaller mean bias and higher pattern correlation than other nine AOGCMs. Based on SRES scenarios, East Asia will experience warmer and wetter climate in the coming 21st century. Changes of geographical patterns from the present to the future are considerably similar through all the scenarios except for the magnitude difference. The temperature in winter and precipitation in summer show remarkable increase. In spite of the large uncertainty in simulating precipitation by regional scale, we found that the summer (winter) precipitation at eastern coast (north of $40^{\circ}N$) of East Asia has significantly increased. In the 21st century, the warming over the continents of East Asia showed much more increase than that over the ocean. Hence, more enhanced (weakened) land-sea thermal contrast over East Asia in summer (winter) will cause strong (weak) monsoon. In summer, the low pressure located in East Asia becomes deeper and the moisture from the south or southeast is transported more into the land. These result in increasing precipitation amount over East Asia, especially at the coastal region. In winter, the increase (decrease) of precipitation is accompanied by strengthening (weakening) of baroclinicity over the land (sea) of East Asia.

• Development and Reproduction
• /
• v.14 no.1
• /
• pp.13-19
• /
• 2010

We developed a novel dicistronic system for the expression of target cDNA sequences in the milk of transgenic animals using goat beta-casein/hGH fusion construct, pGbc5.5hGH (Lee, 2006) and internal ribosome entry site (IRES) sequences of encephalomyocarditis virus (EMCV). Granulocyte colony-stimulating factor (hG-CSF) cDNA was linked to 3' untranslated region of hGH gene in the pGbc5.5hGH via EMCV IRES sequences. Transgenic mice were generated by microinjection and transgene expression was examined in the milk and mammary gland of transgenic mice at 10 days of lactation. Northern blot analysis showed that hGH gene and hG-CSF cDNA were transcribed as a single dicistronic mRNA. The hG-CSF and hGH proteins were independently translated from the dicistronic mRNA and secreted into the milk of transgenic mice. The highest concentration of hG-CSF and hGH in the milk of transgenic mice were $237{\mu}g/m{\ell}$ and $8,990{\mu}g/m{\ell}$, respectively. In contrast, another hG-CSF expression cassette, in which hG-CSF genomic sequences were inserted into a commercial milk-specific expression vector (pBC1), generated a lower level ($91{\mu}g/m{\ell}$) of hG-CSF expression in the milk of transgenic mice. These results demonstrated that the novel pGbc5.5hGH-based dicistronic construct could be useful for an efficient cDNA expression in the milk of transgenic animals.

• The Journal of the Korea Contents Association
• /
• v.12 no.3
• /
• pp.391-400
• /
• 2012

The primary purpose of this paper was carried out to investigate the casual relationship between the servicescape factors and customer`s voluntary behavior, and revisit intention on the wine specific restaurant. For this purpose, a research was developed based on the relevant literature reviews. Data have been collected from 103 a real economic actors who was visited in Daejeon region and were tested by various statistical methods. The results of this empirical study was summarized as follows. The relations between the quality of servicescape of the wine specific restaurant and customer satisfaction have an effects on revisit intention significantly. As a result, a satisfied customers have more intention to revisit the wine specific restaurant. In contrast, there is no significant relationship between servicesacpe and revisit intention in the wine specific restaurant. Therefore, restaurants were required to provide to suitable servicescape by suggesting customer`s revisit inducement strategy. This study of servicescape and customer satisfaction in the wine specific restaurant can provide useful information to growing the wine market and wine specific restaurant specifically.