• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.5
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• pp.768-775
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• 2015

Seasonal variability in the marine seaweed community structure was examined in the intertidal zones at nine study sites in Hallyeohaesang National Park, on the southern coast of Korea from March to October 2014. A total of 145 seaweeds were indentified, comprising 15 green, 41 brown and 89 red algae. Coarsely branched seaweeds were the dominant functional group, comprising 58.95% in species number, whereas filamentous, sheet, thick leathery, crustose and jointed calcareous forms comprised 2.63-17.72% each. The seaweed biomass averaged 358.00 g dry wt/m² and it was maximal at Somaemuldo (847.64 g dry wt/m²) and minimal at Gamam (56.51 g dry wt/m²). Based on biomass, the dominant and subdominant seaweeds were Ulva australis at Gamam, Sargassum thunbergii at Sangju, Ulva australis and S.fulvellum at Neukdo, S.horneri at Dala-Bijindo-Somaemuldo, S. thunbergii at Dapo, and Corallina pilulifera at Songdo. Community indices were as follows: dominance index (DI), 0.43-0.71; richness index (R), 8.26-16.50; evenness index (J'), 0.36-0.54; and diversity index (H'), 1.57-2.19. In conclusion, we found that both biomass and the community structure of seaweeds in Hallyeohaesang National Park were similar to those in other studies of the Southern Sea along the Korean peninsula, and that Hallyeohaesang National Park is a relatively favorable habitat for seaweeds. Future studies should examine the changes in seaweed composition and biomass as they relate to climate change and environmental pollution.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.6
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• pp.732-739
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• 2011

Marine algal flora and community structure were examined at four sites at Tongyeong, Korea, from February to December 2009. Eighty-two algal species were identified, including 10 green, 25 brown, and 47 red algae. During the study period, the number of species was highest at the Dukpo (61 species) site followed by the Gajodo (60 species), Yepo (58 species), and Chilchundo (53 species) sites. Seaweed biomass ranged from 578.11 to 678.26 g, with the maximum and minimum at Chilchundo and Yepo, respectively. Dominant species in term of biomass were Sargassum thunbergii at Yepo, Dukpo, and Gajodo and Gelidium amansii at Chilchundo. The vertical distribution from the high to low intertidal zone was Sargassum thunbergii and Chondria crassicaulis; Sargassum thunbergii and Ulva linza; Ulva pertusa, Sargassum fulvellum, and Gelidium amansii. Two groups clearly segregated in a cluster analysis, a Yepo/Dukpo group (group A) and a Gajodo/Chilchundo (B), indicating relatively strong differences in similarity. The seaweed community structure of group A was characterized by higher biomass and higher species richness of brown algae than were observed in group B. Additionally, the biomass ratio and the species richness of green algae was lower in group A. These differences in seaweed community structure may have been resulted from the effects of cold effluents.

• Microbiology and Biotechnology Letters
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• v.49 no.1
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• pp.88-94
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• 2021

The seaweed, Gracilaria verrucosa (red seaweed) was fermented to produce bioethanol. Optimal thermal acid hydrolysis conditions were determined as 200 mM H₂SO₄ and 10% (w/v) seaweed slurry at 130℃ for 60 min yielding 47.5% of pretreatment efficiency (E_p). After the thermal acid hydrolysis, enzymatic saccharification was carried out with 16 U/ml Viscozyme L, Cellic CTec2 or mixture of Viscozyme L and Cellic CTec2 to G. verrucosa hydrolysates. Enzymatic saccharifications with Viscozyme, Cellic CTec2 or mixture of those yielded 7.3 g/l glucose with efficiency of saccharification, E_s = 34.9%, 11.6 g/l glucose with E_s = 64.4% and the mixture of those 9.6 g/l glucose with E_s = 56.6%, respectively. Therefore, based on the E_s value, Cellic CTec2 was selected for the optimal enzyme for enzymatic saccharification of G. verrucosa hydrolysate. The ethanol productions with non-adapted S. cerevisiae CEN-PK2 (wild type) and S. cerevisiae CEN-PK2 with adaptive evolution to galactose produced 8.5 g/l ethanol with Y_EtOH = 0.19 and 21.5 g/l ethanol with Y_EtOH = 0.50 at 144 h, respectively. From these results, the ethanol production by S. cerevisiae with adaptive evolution showed high concentration of ethanol production using G. verrucosa as a substrate.

• Korean journal of food and cookery science
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• v.31 no.4
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• pp.465-476
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• 2015

The purpose of this study was to develop sodium reduced foods for 10 representative high sodium foods often served in the food service industry, and to conduct sensory evaluation on the foods. The foods are kimchi, cucumber salad, banquet noodle, seaweed soup, ahuk soup, pork kimchi stew, red pepper paste pork chops, beef bulgogi, grilled mackerel and saury stew selected based on data from the 2010 national health and nutrition examination survey. The sodium contents of chinese cabbage kimchi were 688.1 mg in the control, 587.3 mg in the 15% reduced sodium sample group (level 1), and 486.5 mg in the 30% reduced sodium sample group (level 2). The corresponding sodium contents of cucumber salad were 406.4 mg, 345.5 mg, and 284.6 mg. The sodium contents of banquet noodle were 1080.2 mg, 912.2 mg, and 765.8 mg, respectively. The sodium contents of seaweed soup were 459.4 mg, 392.1 mg, and 333.0 mg, respectively. The sodium contents of ahuk soup were 615.3 mg, 534.9 mg, and 434.4 mg respectively. The sodium contents of pork kimchi stew were 1156.2 mg, 988.3 mg, and 820.2 mg respectively. The sodium contents of grilled mackerel were 624.6 mg, 557.4 mg, and 456.9 mg respectively. The sodium contents of red pepper paste pork chops were 723.7 mg, 615.0 mg, and 505.3 mg, respectively. The sodium contents of beef bulgogi were 678.3 mg, 561.9 mg, and 473.3 mg, respectively. The sodium contents of saury stew were 676.0 mg, 574.6 mg, and 470.9 mg respectively. Sensory evaluation was conducted with a total of 30 samples consisting of 10 control food groups, 15%, and 30% reduced sodium food groups. Results showed sodium reduction up to level 1 or 2 in chinese cabbage kimchi, cucumber salad, pork kimchi stew, red pepper paste pork chops, beef bulgogi, grilled mackerel and saury stew. However, the soups and noodles showed significant differences between the control and the 15% reduced sodium (level 1) food groups, specifically in banquet noodle, seaweed soup, ahuk soup.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.5
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• pp.621-628
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• 1993

Total lipid contents from 10 species of marine algae(2 green, 5 brown, and 3 red algae) collected from different places in Korea were examined and their fatty acid compositions were compared among species and habitats. Total lipid(TL) was prominent in green laver(about 7.3~10.1%) of the green algae, in sea mustard and seaweed fusiforme(about 3.1~4.8%) of the brown algae and purple laver(about 4.9~6.4%) of the red algae. Sea mustard and seaweed fusiforme collected at Chungmu contained a relatively high level of TL than that at Yosu and Kijang. The TL content of purple laver showed the highest portion in that collected at Nakdong. Green algae comprised the majority of n-3 fatty acids(29.0~66.3%), which mainly consisted of 16 : 4(n-3) (or 16 : 3 (n-3)), 18 : 3(n-3) and 18 : 4(n-3). Brown algae accounted for a low level of n-3 fatty acids(17.9~36.5%) mainly 18 : 4(n-3), 18 : 3(n-3) and 20 : 5(n-3), whereas the brown algae contained a significant level of n-6 fatty acids(7.23~26.5%) such as 20 : 4(n-6) and 18 : 2(n-6). In the case of red algae, the n-3 fatty acids consisted mostly of 20 : 5(n-3) which scored 53% of polyenoic acids in purple laver collected at Nakdong. The proportion of n-3 fatty acids in algae belonging to the same species was higher in algae of high TL contents. Consequently, TL and n-3 fatty acid levels from the seaweeds studied in this paper were different from their habitats.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.10
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• pp.4311-4317
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• 2014

Background: Nano-biotechnology is recognized as offering revolutionary changes in the field of cancer therapy and biologically synthesized gold nanoparticles are known to have a wide range of medical applications. Materials and Methods: Gold nanoparticles (GNPs) were biosynthesized with an aqueous extract of the red alga Corallina officinalis, used as a reducing and stabilizing agent. GNPs were characterized using UV-Vis spectroscopy, transmission electron microscopy (TEM), energy dispersive analysis (EDX) and Fourier transform infra-red (FT-IR) spectroscopy and tested for cytotoxic activity against human breast cancer (MCF-7) cells cultured in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum, considering their cytotoxicty and effects on cellular DNA. Results: The biosynthesized GNPs were $14.6{\pm}1nm$ in diameter. FT-IR analysis showed that the hydroxyl functional group from polyphenols and carbonyl group from proteins could assist in formation and stabilization. The GNPs showed potent cytotoxic activity against MCF-7 cells, causing necrosis at high concentrations while lower concentrations were without effect as indicated by DNA fragmentation assay. Conclusions: The antitumor activity of the biosynthesized GNPs from the red alga Corallina officinalis against human breast cancer cells may be due to the cytotoxic effects of the gold nanoparticles and the polyphenolcontent of the algal extract.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.3
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• pp.270-278
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• 1995

This report described on the soluble, insoluble, and total dietary fiber level depending on the harvesting seasons and habitats in 9 species of marine algae. Total dietary fiber contents were comprised $25.4-38.1\%$ (dry basis) in green laver and $35.4-43.8\%$in sea staghorn of green algae, $34.2-48.8\%$ in sea mustard, $37.5-47.8\%$ in seaweed fusiforme, $42.9-71.3\%$ in gulf weed, and $37.1-45.1\%$ in sea tangle of brown algae, and $31.3-40.5\%$ in laver, $51.5-60.4\%$ in seaweed dilatata, and $57.1-65.8\%$ in seaweed furcata of red algae. Relatively high levels of both soluble and insoluble dietary fibers were found in seaweed furcata and gulf weed. The ratio of soluble dietary fiber to total dietary fiber was the highest in green laver $(43.7-64.8\%)$, sea mustard $(17.5-31.3\%)$, and seaweed furcata $(44.7-63.2\%)$ in their respective groups. The highest level of algal polysaccharides was confirmed to be an alkali-soluble alginic acid $(9.0-15.1\%)$ in whole brown algae, porphyran$(5.8\%)$ in laver, agar $(20.0\%)$ in seaweed furcata, and carrageenan $(23.8\%)$ in seaweed dilatata of red algae.

• ALGAE
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• v.17 no.2
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• pp.127-133
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• 2002

The red seaweed Eucheuma serra is a high yielding source of lectins. The plants were collected from a depth of 5-6 meters and cultured in the laboratory, field and deep seawater. A Daily Growht Rate (DGR) of 3.5% was observed at 18℃ with a low light of 30μmol photon $ m^{-2} · s^{_1}$ in the laboratory. When the plants were cultured in the field at different depths during winter onths of December and January, best growth was observed at 1 m depth and a DGR of 2.14±0.04% was recorded. The plants grown in the tank with a continuous supply of deep seawater showed a DGR of 8.2% The results indicate that E. serra can be cultivated in large scale both in deep seawater in the tank and in the field for the extraction of lectins at a commercial scale.

• ALGAE
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• v.30 no.3
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• pp.217-222
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• 2015

Pythium species (Pythiales, Oomycetes) are well known as the algal pathogen that causes red rot disease in Pyropia / Porphyra species (Bangiales, Rhodophyta). Accurate species identification of the pathogen is important to finding a scientific solution for the disease and to clarify the host-parasite relationship. In Korea, only Pythium porphyrae has been reported from Pyropia species, with identifications based on culture and genetic analysis of the nuclear internal transcribed spacer (ITS) region. Recent fungal DNA barcoding studies have shown the low taxonomic resolution of the ITS region and suggested the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene as an alternative molecular marker to identify Pythium species. In this study, we applied an analysis of both the ITS and cox1 regions to clarify the taxonomic relationships of Korean Pythium species. From the results, the two closely related Pythium species (P. chondricola and P. porphyrae) showed the same ITS sequence, while the cox1 marker successfully discriminated P. chondricola from P. porphyrae. This is the first report of the presence of P. chondricola from the infected blade of Pyropia yezoensis in Asia. This finding of the algal pathogen provides important information for identifying and determining the distribution of Pythium species. Further studies are also needed to confirm whether P. chondricola and P. porphyrae are coexisting as algal pathogens of Pyropia species in Korea.

• Advances in Traditional Medicine
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• v.7 no.4
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• pp.341-347
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• 2007

Red seaweed (Callophyllis japonica) has long formed part of the diet of Asians, but the pharmacological properties of this plant have not been evaluated. In this study, we examined the effect of an ethanol extract of C. japonica on the generation of nitric oxide (NO) in RAW 264.7 cells. The C. japonica extract increased the generation of NO and tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), which were detected by the Griess method and an enzyme-linked immunosorbent assay, respectively. The increased production of NO by C. japonica extract was inhibited by $N^G$-monomethyl-L-arginine ($100{\mu}M$), a specific inhibitor of NO production in the L-arginine-dependent pathway, and by the nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) inhibitor, pyrrolidine dithiocarbamate ($10-100{\mu}M$) in a dose-dependent manner. These findings demonstrate that C. japonica extract stimulates the production of NO and $TNF-{\alpha}$ in RAW 264.7 cells through the activation of $NF-{\kappa}B$ and that this extract might also inhibit the growth of the human leukemic cells.