• Journal of Ginseng Research
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• v.28 no.3
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• pp.127-131
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• 2004

This study was carried out to investigate the effect of red ginseng water extract (RGWE) on trypsin activity. After extraction of fat soluble and saponin component from red ginseng powder by methyl alcohol, the residue was extracted with distilled water, and manufactured to water extract. The extract was dialyzed with different molecular cut off membrane. Trypsin activity demonstrated the highest level at the RGWE concentration of 9${\times}$10$\^$-2/% in reaction mixture, and also increased to 15% at 2.9${\times}$10$\^$-3/%. Km value was decreased and Vmax was increased in the present of red ginseng water extract. Red ginseng water extract was partially purified by dialysis, Bio-Gel P-I0 and DEAE-cellulose column chromatography. The active fraction demonstrated positive reaction to ninhydrin, DNS and folin reaction.

• Journal of Life Science
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• v.29 no.9
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• pp.949-954
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• 2019

Membrane topology is a key characteristic of membrane proteins. We previously reported the cloning of the chromosome 4 open-reading frame 32 (C4orf32) gene as a potential membrane protein; however, the cellular localization and membrane topology of C4orf32 was as yet unknown. In this study, we found that green fluorescent protein (GFP) fused to the C-terminus of C4orf32 (C4orf32-GFP) was localized to the endoplasmic reticulum (ER). We applied three tools to identify determinants of C4orf32 topology: protease protection, fluorescence protease protection (FPP), and an inducible system using the ternary complex between FK506 binding protein 12 (FKBP), rapamycin, and the rapamycin-binding domain of mTOR (FRB) (the FRB-rapamycin-FKBP system). Using protease protection and FPP assays, we found that the GFP tag in C4orf32-GFP was localized to the cytoplasmic surface of the ER membrane of HeLa cells. Protease protection and FPP assays are useful and complimentary tools for identifying the topology of GFP fusion membrane proteins. The FRB-rapamycin-FKBP system was also used to study the topology of C4orf32. In the absence of rapamycin, a monomeric red fluorescent protein-FKBP fusion (mRFP-FKBP) and C4orf32-GFP-FRB were localized to the cytoplasm and the ER membrane, respectively. However, in the presence of rapamycin, the mRFP-FKBP was shifted from the cytoplasm to the ER and colocalized with the C4orf32-GFP-FRB. These results indicate that the FRB moiety is facing the cytoplasmic surface of ER membrane. Overall, our results clearly suggest that C4orf32 belongs to the family of type I ER resident membrane proteins.

• Membrane and Water Treatment
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• v.9 no.5
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• pp.309-315
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• 2018

The applicability of the ultrafiltration process for color removal from dye-containing water has been examined in this study. The optimization of major process variables, such as dye concentration, chitosan concentration and transmembrane pressure on permeate flux and color removal efficiency was investigated. To find the most appropriate results for the experiment, the Box-Wilson experimental design method was employed. The results were correlated by a response function and the coefficients were determined by regression analysis. Permeate flux variation and color removal efficiency determined from the response functions were in good agreement with the experimental results. The optimum conditions of chitosan concentration, dye concentration and pressure were 50 mg/l, 50 mg/l and 3 bars, respectively for the highest permeate flux. On the other hand, optimum conditions for color removal efficiency were determined as 50 mg/l of dye concentration, 50 mg/l of chitosan concentration and 1 bar of pressure.

• Ocean Science Journal
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• v.41 no.4
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• pp.221-226
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• 2006

More attention has been paid to the research on decolorization of dyeing wastewater nowadays. In this study, an investigation into the decolorization of dyeing wastewater was conducted using a combination of coagulant, carboxymethyl chitosan (NOCC) and coagulant aid, polyscrylamide (PAM). The factors influencing the decolorization efficiency, such as pH value, coagulant and the dosages of coagulant, were discussed. The results showed that using PAM as coagulant aid could reach a high decolorization efficiency compared with using NOCC alone. The optimal conditions were pH 2.3, 480 mg/L for NOCC, and 4-8 mg/L for PAM. Under the optimum conditions, the rate of decolorization could achieve 99%, and the removal of chemical oxygen demand (COD) could achieve 90%. In addition, the membrane processes with chitosan/rare-earth-metals could enhance the decolorization rate of Direct Black FF to 94.7%, and Indanthren Red F3B to 98.2%, respectively.

• Membrane and Water Treatment
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• v.5 no.4
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• pp.251-263
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• 2014

The study contributes in the treatment of waste generated by the textile complex cotton of Draa Ben Khedda, Algeria. The azo dye "Direct Red Solophenyle 4GE" represents the base particle of the discharges and electrocoagulation with nanofiltration are used as a means of treatment. The solar photovoltaic is suitable for electrochemical process to reduce the energy cost. Several study parameters are considered in this work. The electrocoagulation batch gives the best reduction 37% for a dye concentration of 7.21 mg/L ($[NaCl]_{added}$=1g/L; $j=25.2mA/cm^2$). Coupling methods (electrocoagulation-nonofiltration) gives a complete discoloration rejecting concentration 52.4 mg/L (pHi = 7.6, $[NaCl]_{added}$=3g/L, $j=2.13mA/cm^2$). The result shows the coupling efficiency with a reduced amount of resulting slurry at the end of treatment.

• Journal of Environmental Science International
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• v.18 no.1
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• pp.15-19
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• 2009

Early studies claimed that heterotrophic dinoflagellates Pfiesteria piscicida and related genera may produce a putative water-soluble toxin that causes death of fish and other marine animals. Several methods were tested to visualize plate morphology of Cryptoperidiniopsis brodyi and Pfiesteria piscicida. Cellulose plates of cells were exposed and visualized- by a membrane stripping method using Triton X-100. While calcofluor M2R white stain could readily bind to the thecal plates, details of the plate tabulation were difficult to observe. Fixation with osmium tetroxide $(OsO_4)$ produced well preserved cells with little morphological distortion, but thecal plates could not be visualized. Scanning electron microscopy (SEM) observation using the membrane stripping method showed distinctive plate tabulations between C. brodyi and P. piscicida suggesting that this method is a useful tool for morphological identification of lightly armored dinoflagellates.

• Membrane and Water Treatment
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• v.11 no.4
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• pp.275-282
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• 2020

Hranfa's marl, a local natural mineral, is selected for the decontamination by adsorption of aqueous effluents in textile industry. Its physicochemical characterization is first performed. It is composed mainly of Calcite, Quartz, Ankerite and Muscovite. Its specific surface area is 40 ㎡ g^-1. Its adsorption performance is then tested in batch conditions using an industrial organic dye, Bemacid Red E-TL, as a model pollutant. The measured adsorption capacity of Hranfa's marl is 16 mg g^-1 which is comparable to that of other types of natural adsorbents. A hybrid process is tested coupling adsorption of the dye on marl in suspension and microfiltration. An adsorption reactor is inserted into the circulation loop of a microfiltration pilot using ceramic membranes. This makes possible a continuous extraction of the treated water provided that a periodic replacement of the saturated adsorbent is done. The breakthrough curve obtained by analyzing the dye concentration in the permeate is close to the ideal one considering that no dye will cross the membrane as long as the adsorbent load is not saturated. These first experimental data provide proof of concept for such a hybrid process.

• Development and Reproduction
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• v.21 no.1
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• pp.63-69
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• 2017

On the 15 days after hatching, the larvae was 4.24-5.10 mm (mean $4.66{\pm}2.18mm$) in total length, and the fins of the membrane started to develop into a fan shape and the melanophore was deposited upper the alimentary canal of the abdomen and on the bladder. At 35 days after hatching, the post-larvae formed a branch-shaped melanophore on the head part with a total length of 6.98-12.5 (mean $9.35{\pm}1.71$) mm, formed on the upper and lower parts of the caudal part, formed on the upper and lower parts of the caudal part, and deposited under the head part and abdomen. At 40 days after hatching, the juvenile was 11.3-18.1 (mean $14.9{\pm}1.53$) mm in total length.

• Journal of Ginseng Research
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• v.18 no.3
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• pp.187-190
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• 1994

The pith and xylem parts of fresh root that turned into inside-white during processing for red ginseng was investigated under scanning electron microscope in comparison with the sa31e position of fresh root processed into normal reddening. In the inside-white part starch storage cells remain mostly in vacancy or with small number of starch granules and with large hollow by missing cell membranes between cells. Many starch seed granules appeared on the surface of storage cell wall in the inside-white part. Fresh root sample showed better picture than dried powder.

• Journal of the Korean Chemical Society
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• v.53 no.2
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• pp.144-151
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• 2009

An optode for cadmium ion determination has been designed by immobilization of dithizone on triacetylcellose membrane. When the optode membrane is introduced into a real samples containing cadmium, there is a color change from green to red, making it possible to use the change in absorbance at 611 nm as the analytical signal. The sensor could be used in the range of 0.3-3 ${\mu}g\;ml^{-1}$ (2.67-26.67 ${\mu}M$) of $Cd^{2+}$ ions with a limit of detection of 0.025 ${\mu}g\;ml^{-1}$ (25 ng $ml^{-1}$). The response time of optode is within 15 min depending on the concentration of $Cd^{2+}$ ions. It can be easily and completely regenerated by dilute EDTA solution. The effect of different possible interfering species has been examined and was shown the optode has a good selectivity. The results obtained for the determination of cadmium ion in polluted soil and water samples using the proposed optode was found to be comparable with the well-established atomic absorption method.