• 한국식품영양학회지
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• 제9권3호
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• pp.319-324
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• 1996

The functional properties of protein recovered from red crab (Chitinonecetes opiiie) processing in water (RCP) were examined and compared with those of soybean protein isolate at pH 2~10 in water and NaCl solu5ion. The solubilities of RCP and SPI were miniumu at pH 4, the isoelectric point and increased significantly at lower or higher than pH 4. Solubilities in NaCl solution for both proteins decreased with incr NaCl concentration increase at all pH ranges. Emulsion capacity for both proteins was also minimum at pH 4 and increased as protein concentration increased from 2 to 6%. Emulsion capacity of RCP was higher than these of SPI at pH 6∼10 and all protein concentrations. Emulsion stability showed a similar trend to that of emulsion capacity. RCP had higher oft absorption capacity and lower water absorption capacity than SPI.

• 한국수산과학회지
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• 제36권1호
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• pp.62-64
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• 2003

Recently, relatively large amount of a new species of crab belonging to genus Chionoecetes that has intermediate characteristics between red-tanner crab (Chionoecetes japonicus) and queen crab (C. opilio) was caught the East Sea of Korea. The new species of crab was tentatively named Neodo-Daege. Amino acid and proximate compositions of the edible meat of red-tanner crab and Neodo-Daege were determine. The proximate composition of the meats of Neodo-Daege were $79.2{\%}$ in moisture, $17.4{\%}$ in protein, $0.6{\%}$ in lipid, and $1.5{\%}$ in ash. Neodo-Daege was higher in protein and lipid contents, and lower in moisture and ash contents than those of red-tanner crab. The total amino acid contents in the meat of Neodo-Daege were higher than those of red-tanner crab, but the profile of amino acids was similar to one another. Among amino acids, glutamic acid, arginine, aspartic acid, lysine and glycine were major components of the crabs.

• 한국식품영양학회지
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• 제9권3호
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• pp.325-330
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• 1996

Foaming capacity (FC) and stability (FS) of protein recovered from red crab (Chitinonecetes opilio) processing in water and soybean protein isolate were determined at pH 2.0~10.0 in water and NaCl solution. The FC values for both proteins showed the lowest values at the isoelectric point (pH 4.0) and increased nth an increase in pH above the isoelectric point. FC of RCP was higher than that of SPI at pH 10.0 in water and both NaCl solutions. FC of SPI increased with an increase in NaCl concentration at pH 4.0 and 6.0, but FC of RCP was not affected. The highest FS values for both proteins were obtained at pH 4.0 in water. At pH 2.0, FC of RCP decreased with NaCl concentration increase, but FS increased. NaCl concentration had little effect on FS of RCP at pH 4.0 and 6.0, but the FS decreased at pH 10.0. FS of SPI was similar to that of RCP at pH 2.0 and increased with NaCl concentration Increase from 0.1 to 0.5M NaCl at pH 10.0.

• Journal of Nutrition and Health
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• 제45권5호
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• pp.429-436
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• 2012

본 연구는 폐기되는 홍게껍질의 단백질을 활용하기 위하여 홍게껍질 분말에 상업적으로 사용하는 6가지 protease를 단일 또는 혼합처리하여 획득한 가용성 가수분해물의 분자량 및 아미노산 조성 등을 비교 분석하여 기능성 소재 및 보충제로서의 활용 가능성을 확인하고자 하였다. 단일효소 6종 중 단백질 분해능은 Biuret assay과 280 nm assay 및 $^{\circ}Brix$로 분석한 결과 Protease A (PA)가 현저하게 높았다. 단일효소 처리로 생성된 가수분해물의 단백질 분자량은 150 kDa 이하의 밴드를 형성하였다. 단일효소 중 단백질 분해능이 가장 강력한 PA의 최적 가수분해 조건을 선정하여 PA에 Protamex (P), Flavourzyme (F), Alcalase (A), Protease M (PM)과 Protease A (PA)를 1대 1의 비로 혼합 (PA + P, PA + PM, PA + F, PA + A)하여 GC로 아미노산 조성을 확인하였다. 총 아미노산 함량은 PA + P 247.411 mg/g > PA + F (206.442 mg/g) > PA + A (133.385 mg/g) > PA + PM (59.131 mg/g) > PA (54.875 mg/g)의 순으로 혼합효소처리가 단일효소처리에 비해 높은 것을 확인할 수 있었다. 혼합효소처리 한 홍게껍질의 아미노산 조성은 사용 효소에 따라 다소 차이가 있었다. 주로 phenylalanine, glycine, alanine, leucine의 함량이 높았으며 tyrosine, cystine은 검출되지 않았다. 쌀을 주식으로 하는 우리나라 사람들에게 부족되기 쉬운 필수 아미노산인 lysine, phenylalnine, leucine, isoleucine의 함량이 높아 아미노산 보충효과를 기대할 수 있을 것으로 보인다.

• 한국식생활문화학회지
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• 제23권6호
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• pp.667-680
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• 2008

The moisture contained in conventionally extracted red crab shell samples was lower than than in samples extracted using high pressure. In contrast, ash content increased as the extraction time increased and displayed no significant variation in high pressure extraction. Extraction time was influential, with lower lightness (L-value) being obtained in samples extracted at high pressure as compared to traditional extraction. However, large variation in a- and b-values resulted from traditional extraction. For both traditional and high pressure extraction increases in pH, salinity and saccharide content were noted with increasing extraction time, but these parameters did not vary substantially in red crab shell extracted at high pressure. The mineral content in samples extracted by high pressure was higher than in traditionally extracted samples. The mineral content increased with time, with marginally higher contents of essential amino acids and flavor enhancing amino acids noted following high pressure extraction. The contents of the flavor enhancing amino acids increased from 30?60 min, thereafter decreasing. Assessment of consumer acceptance revealed that, while traditionally manufactured red crab sauce was popular, sauce manufactured using a 60 min high pressure extraction was preferred. Extending the high pressure extraction time to 75 min produced a less sensory acceptable product, due to higher mineral and protein levels.

• 한국수산과학회지
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• 제49권3호
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• pp.285-292
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• 2016

In this study, we investigated the quality characteristics of noodles supplemented with lyophilized leg-meat powder from the red snow crab, Chionoecetes japonicus (CP). The noodles were prepared by adding 0, 2, 4, 6, 8, 10% of CP on a wheat-flour basis. The ash, crude protein and crude lipid contents of noodles increased with increasing CP content. The cooked weight, volume and water absorption values of noodles decreased, whereas the turbidity increased, with increasing CP content. The L values of the noodles decreased with increasing CP content, whereas the a and b color values increased. The hardness, gumminess and chewiness values increased with increasing CP content increased, while springiness and cohesiveness showed no significant differences among the groups. In the sensory evaluation, cooked noodles with 4-6% CP had greater overall acceptability than the noodles with other CP levels.

• Fisheries and Aquatic Sciences
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• 제20권7호
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• pp.13.1-13.7
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• 2017

In order to evaluate the maximal storable period of the raw crab for a non-thermal muscle separation, the quality changes of the leg meat of red snow crab (Chionoecetes japonicus) during freeze storage were investigated. Fresh red snow crabs were stored at $-20^{\circ}C$ for 7 weeks, and the leg muscle was separated by a no heating separation (NHS) method every week. During the storage, considerable loss of the leg muscle did not occur and microbiological risk was very low. In contrast, discoloration appeared at 2-week storage on around carapace and the leg muscle turned yellow at storage 3-week. In physiochemical parameters, protein and free amino acids gradually decreased with storage time, expected that proteolytic enzymes still activated at $-20^{\circ}C$. At 4-week storage, the sensory acceptance dropped down below point 4 as low as inedible and notable inflection points in pH and acidity were observed. The volatile base nitrogen was low, though a little increase was recorded. These results suggested that the maximal storable period at $-20^{\circ}C$ of the raw material was within 2 weeks and it was depended on external factor such as the discoloration. The present study might be referred as basic data for approaches to solve quality loss occurred in non-thermal muscle separation.

• Fisheries and Aquatic Sciences
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• 제8권4호
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• pp.195-200
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• 2005

This study optimized the mixing ratios of seafood sauce with blue crab for maximal sensory attributes using a response surface methodology with central composite design. It evaluated sensory appeal, nutritional value, and some rheological properties. The optimal formulation appeared to be 252 g of tomato paste, 78 g of chopped garlic, and 519 g of blue crab block; this ratio resulted in a predicted sensory score of 5.7 (on a 7-point scale) for overall acceptability. Addition of blue crab block increased moisture, ash, and protein contents in various sauces, but fat contents were similar in every sauce. Major free amino acids such as glutamic acid, phospho serine, asparagine, and arginine composed about $60\%$ of the total amino acid content. Standard and optimized sauces were red and light yellow, and those color values were significantly higher than in American-style sauces. Optimal viscosity was half the value of American-style sauces; more blue crab block added to the sauce resulted in lower viscosity. Adding this sauce to seafood dishes will likely aid development of flavoring substances.

• 한국식품영양과학회지
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• 제34권7호
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• pp.1077-1081
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• 2005

붉은 대게는 소비자의 기호도가 높으나 생산량이 적고, 감소 추세에 있으며 수율이 낮아 이의 완전 이용이 절실하다. 이러한 일면에서 붉은 대게를 보다 효율적으로 이용하고자 붉은 대게 페이스트(paste)의 식품성분 특성 및 게 연제품의 소재로서의 이용가능성을 검토하였다. 붉은 대게 페이스트는 육에 비하여 단백질 함량은 낮았고, 칼슘과 같은 기능성 성분이 다량 함유된 회분 및 조지방은 높았으며, 휘발성 염기질소 함량은 8.4mg/100g으로 가공 원료로 이용 가능한 범위이었다. 또한, 붉은 대게 페이스트의 경우 육에 비하여 구성아미노산 및 지방산 조성은 크게 차이가 없었고, 칼슘함량은 휠씬 많았다. 붉은 대게 페이스트를 surimi에 대하여 $15\%$ 첨가하여 연제품을 제조한 다음, 이를 무첨가 제품에 대하여 색조, 조직감 및 향을 비교한 결과 색조 및 향의 경우 첨가 제품이 우수하였고, 조직감의 경우 두 제품간에 차이가 없었다. 이상의 결과 게 페이스트는 게 연제품의 기호성 증대 이외에 영양 및 기능성 강화 소재로 사용 가능하리라 판단되었다.

• 한국조리학회지
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• 제23권2호
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• pp.56-63
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• 2017

본 연구는 홍게의 부산물인 껍질을 이용하여 제조한 데리야끼 소스의 이화학적 특성, 관능적 특성을 평가하였다. 수분은 튀김 처리한 FRC 시료가 가장 높았으며, 수침 처리한 SOC 시료가 가장 낮았고, 점도는 이와 반대되는 경향이었다. pH는 건조 처리한 DRC 시료가 가장 높았고, 전처리하지 않은 RAC 시료가 가장 낮았다. 색도에서는 L, a, b값 모두에서 튀김처리한 FRC 시료가 가장 높았으며, 기름에 튀기는 전처리 과정을 거치면서 지용성 색소 단백질인 아스타잔틴이 더 용출되어 L, a, b 값 모두에 영향을 준 것으로 사료된다. 염도는 튀김 처리한 FRC 시료가 가장 높았고, 수침 처리한 SOC 시료가 가장 낮았다. 가용성 고형분 함량은 이와 반대되는 경향이었다. 총 유리 아미노산의 함량은 수침 처리한 SOC 시료가 장 높았으며, 전처리하지 않은 RAC 시료가 가장 낮았다. 유리 아미노산 중 필수 아미노산과 맛난 맛 아미노산도 SOC 시료가 가장 높게 측정되어 전처리 방법을 달리한 시료 중 가장 감칠맛이 높은 것을 알 수 있었다. 기호도 검사 결과, 유리 아미노산 함량이 가장 높았던 SOC 시료가 외관, 향, 맛, 텍스쳐, 전체적인 기호도 항목에서 가장 높은 기호도를 보였다. 이것으로 보아 전처리 방법 중 홍게 껍질의 침지 처리 과정은 가용성 고형분 및 맛난 맛 성분의 용출이 높아지면서 데리야끼 소스의 전체적인 맛의 기호도에 긍정적인 영향을 주는 것을 알 수 있었다. 이상의 실험결과를 종합해 볼 때, 홍게 껍질을 이용하여 데리야끼 소스를 제조하는데 있어 침지 처리하는 전처리 공정을 거치는 것이 영양적, 관능적으로 우수한 데리야끼 소스의 제조가 가능한 것으로 사료된다.