• Clinical and Experimental Reproductive Medicine
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• v.43 no.1
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• pp.9-14
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• 2016

Objective: Autophagy contributes to the clearance and recycling of macromolecules and organelles in response to stress. We previously reported that vitrified mouse oocytes show acute increases in autophagy during warming. Herein, we investigate the potential role of Atg7 in oocyte vitrification by using an oocyte-specific deletion model of the Atg7 gene, a crucial upstream gene in the autophagic pathway. Methods: Oocyte-specific Atg7 deficient mice were generated by crossing Atg7 floxed mice and Zp3-Cre transgenic mice. The oocytes were vitrified-warmed and then subjected to in vitro fertilization and development. The rates of survival, fertilization, and development were assessed in the Atg7 deficient oocytes in comparison with the wildtype oocytes. Light chain 3 (LC3) immunofluorescence staining was performed to determine whether this method effectively evaluates the autophagy status of oocytes. Results: The survival rate of vitrified-warmed $Atg7^{f/f}$;Zp3-Cre ($Atg7^{d/d}$) metaphase II (MII) oocytes was not significantly different from that of the wildtype ($Atg7^{f/f}$) oocytes. Fertilization and development in the $Atg7^{d/d}$ oocytes were significantly lower than the $Atg7^{f/f}$ oocytes, comparable to the $Atg5^{d/d}$ oocytes previously described. Notably, the developmental rate improved slightly in vitrified-warmed $Atg7^{d/d}$ MII oocytes when compared to fresh $Atg7^{d/d}$ oocytes. LC3 immunofluorescence staining showed that this method can be reliably used to assess autophagic activation in oocytes. Conclusion: We confirmed that the LC3-positive signal is nearly absent in $Atg7^{d/d}$ oocytes. While autophagy is induced during the warming process after vitrification of MII oocytes, the Atg7 gene is not essential for survival of vitrified-warmed oocytes. Thus, induction of autophagy during warming of vitrified MII oocytes seems to be a natural response to manage cold or other cellular stresses.

• Journal of the Korean Dietetic Association
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• v.14 no.1
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• pp.51-63
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• 2008

The purpose of this study was to understand the management status of food wastes in school meal to find the effective ways that would reduce and recycle them. Data was collected using questionnaires from 223 school dietitians located in Jeonbuk area. The results were as followed : 1) Waste was managed by the dietitians(54.7%) and cook(42.2%). The waste disposal contract was made by dietitians(65.0%), executives(31.4%). Therefore, school dietitians had primary responsibilities in the waste management. 2) As disposal ways, 48.9% processed municipal solid waste and food waste together and 46.6% processed separately. 3) A half of schools(52.9%) produced food wastes weighed under 10$\sim$15kg daily and using food wastes as animal food for farms was most popular method to dispose(87.4%). 4) Reasons to have food-wastes were disliking the taste of menu(50.2%) and the inappropriate ways of dealing with remaining food(64.1%) and donating to food bank(33.6%) in order. 5) The suggested ways to reduce the food-waste was to improve on cooking method(72.2%), changing of portion size(61.0%), measuring the amount of leftover food(53.4%) and requesting cooperation to educators(52.9%). 6) The reported consequences of reducing the food waste were: lowered grocery expenses(46.2%), and inspiring students on helping environmental preservation(23.8%). Therefore, the administration should work on finding a method to reduce food-waste and a way to use these resources efficiently. It is important to induct student's attitude on recycling remaining products by using food bank via nutrition education. Dietitian also should consider to reduce the leftover of food by using a standardized recipe.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.1
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• pp.1-6
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• 2004

Agricultural recycling of livestock wastes by composting has lots of beneficial effects on crop production and soil fertility. Most of composts are made from pig manure and water content controller such as saw dust, bark, rice hulls etc. by aerobic processing. But the insufficient supply of saw dust, bark etc. cause the indiscreet use of industrial waste having heavy metals and toxic synthetic chemicals. This research investigated the present quality status of organic by-product fertilizers, and suggested the way of quality interpretation under the statistical approaches based on median, mean and weighted average value. Since the data of one hundred of samples for heavy metal contents (Cd, Pb, Cu, and Cr) and OM/N showed extremely left-skewed distribution, the median was more useful than the mean in representing the characteristics of distribiition for each items. The weighted average value will be a useful index for the quality based on the total amount of producing.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.25 no.2
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• pp.42-53
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• 2020

In this study, heavy metal in road-deposited sediments (RDS) and marine sediment around Gwangyang Bay area have been investigated to assess the pollution status of metals and to understand the environmental impact of RDS as a potential source of metal pollution. Zn concentration for <63 ㎛ size fraction was the highest (2,982 mg/kg), followed by Cr, Ni, Pb, Cu, As, Cd, and Hg. Metal concentrations in RDS increased with decreasing particle size and relatively higher concentrations were observed around the metal waste and recycling facilities. For particle size in RDS smaller than 125 ㎛, EF values indicated that Zn was very high enrichment and Cr, Cd, Pb were significant enrichment. The concentrations of metals in marine sediments were mostly below the TEL value of sediment quality guidelines of Korea. However, the Zn concentrations has increased by 30~40% compared to 2010 year. The amounts of Zn, Cd and Pb in less than 125 ㎛ fraction where heavy metals can be easily transported by stormwater runoff accounted for 54% of the total RDS. The study area was greatly affected by Zn pollution due to corrosion of Zn plating materials by traffic activity as well as artificial activities related to the container logistics at Gwangyang container terminal. The fine particles of RDS are not only easily resuspended by wind and vehicle movement, but are also transported to the surrounding environments by runoff. Therefore, further research is needed on the adverse effects on the environment and ecosystem.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• 2006.11a
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• pp.597-601
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• 2006

There are considerable amount of man power and time spent for analysis and selection of various materials due to the inadequate application system and rapid searching process of material related information in each stage of building production process. Also, due to the insufficiency in recycling system of important information created for each process, they are hoarded at each task stage and project completion. As a result, because of the repetition of the similar/identical tasks on each process, it is the major reason for impeding the efficient employment of manpower of the company and decreasing competition of the company. Moreover, in selecting finish material in the project field, there are many difficulties due to the lack of technical information such as product characteristics and field applications. The construction quality also affects the profit and loss of the product which calls for a need to develop continued information management system that the finish material related examples could be shared in real time. The objective of this study is to propose a building finish material information which the real time research and application is possible to raise the productivity. TO do this, the current material information task status analysis and questionnaire research should be conducted to understand the demand of system development and reflect the result onto the system for easy access and application. Therefore, the building finish material information system for enhancement of productivity of construction task proposed in this study is expected to be utilized in enhancement of construction quality, maximization of company profit, and strengthening of company competition.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.12
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• pp.140-149
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• 2017

Recently, it has become necessary to consider climate change when managing multi-purpose river functions. However, in terms of domestic rivers, the management of national and local rivers is separated and the river information cannot be integratedly handled. Especially, it is not sufficient to collect and update information by recycling reports for design and construction. In addition, the basic information of the rivers is dependent on the GIS-based RIMGIS system, but the reliability of the information is deteriorating due to the construction of spatial information using the river basement planning results. The purpose of this study is to investigate the current status of the information system with regard to the maintenance and operation of the river facilities. Through the verification of actual cases, the optimal solution was suggested from the point of view of practical information. As a result, we constructed an information system for the reliable maintenance of river facilities and examined the integrated information management plan. The results of this study can be used to improve the existing information and technical and institutional procedures for the integrated maintenance and operation of river facilities. It will be helpful to introduce the BIM as well as solve to the information gap with other fields through the establishment of an information framework to improve the information construction of river areas.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.25 no.4
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• pp.79-108
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• 2014

The purpose of this study was to research instances of green library construction and recognition in order to increase awareness of green libraries. To this end, we investigated the green library's outline and green building, evaluated cases of G-SEED certified libraries, and surveyed the employees at the 16 different G-SEED certified libraries. Results showed that the employees' knowledge of green libraries and the green certification system was low, whereas their interest and needs regarding the green library concept were very high. Second, the respondents were able to recognize as a whole the factors of green library construction based on the evaluation items for G-SEED and place particular emphasis on 'recyclable resources & recycling bins.' However, only a few recognized 'water reclamation/reuse system establishment' as a green library factor. Third, the respondents largely agreed upon an evaluation of items on the suitability of G-SEED, and their ranked reasons for build a green library were preventing environmental pollution, energy-saving, environment, ecology, and indoor environmental factors. Fourth, a lack of 'awareness about the concept of the green library' was thought to most affect the awareness of difficulties in green library construction, and the respondents agreed with the need for 'library image enhancement' to better manage expectations of the green library.

• Molecules and Cells
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• v.38 no.1
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• pp.40-50
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• 2015

In the interaction between plants and pathogens, carbon (C) resources provide energy and C skeletons to maintain, among many functions, the plant immune system. However, variations in C availability on pathogen associated molecular pattern (PAMP) triggered immunity (PTI) have not been systematically examined. Here, three types of starch mutants with enhanced susceptibility to Pseudomonas syringae pv. tomato DC3000 hrcC were examined for PTI. In a dark period-dependent manner, the mutants showed compromised induction of a PTI marker, and callose accumulation in response to the bacterial PAMP flagellin, flg22. In combination with weakened PTI responses in wild type by inhibition of the TCA cycle, the experiments determined the necessity of C-derived energy in establishing PTI. Global gene expression analyses identified flg22 responsive genes displaying C supply-dependent patterns. Nutrient recycling-related genes were regulated similarly by C-limitation and flg22, indicating re-arrangements of expression programs to redirect resources that establish or strengthen PTI. Ethylene and NAC transcription factors appear to play roles in these processes. Under C-limitation, PTI appears compromised based on suppression of genes required for continued biosynthetic capacity and defenses through flg22. Our results provide a foundation for the intuitive perception of the interplay between plant nutrition status and pathogen defense.

• Journal of the Korean Society for Library and Information Science
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• v.53 no.2
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• pp.91-116
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• 2019

This study conducted a demand analysis for the operation and management of closed schools, the direction of future use of closed schools, and the utilization of closed schools, among local residents expected to play a key role in the successful use of closed libraries. According to the study, residents in areas where schools actually occur may actively consider the use of closed schools when they are lacking in the number of libraries in the region or when they hope to remodel their schools as libraries, given that they are positive about future use of closed schools and that remodeling them is more economical in terms of cost than building new libraries. The current awareness of the use and operation of closed schools remains at a normal level, so it is deemed necessary to improve the direction of the promotion, operation and recycling of closed schools in the order of promotion, operation and physical aspects by grouping obstacles to the operation and management of closed schools. In addition, the current status of cultural facilities and the demands of local residents are met in terms of the types of use of closed school facilities, and it means that closed schools can be used in a relatively easy-to-access location based on their location, while libraries can be used as a space for cultural facilities that are different from the existing public libraries, exhibition halls, and eco-friendly libraries.

• The Journal of Engineering Geology
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• v.33 no.4
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• pp.737-753
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• 2023

Abandoned mines represent unused space resulting from resource use and changes in industrial environments. Efforts are underway to repurpose such underground spaces, leveraging their unique attributes of temperature stability, shading, and security. This study aimed to assess the feasibility of operating high-demand data centers and public sports facilities as potential recycling options for abandoned mine spaces. The status of data centers located in abandoned mines abroad was examined, including their operational technology capitalizing on the advantages of underground spaces. Considering the varying sizes of underground spaces in different types of abandoned mine in South Korea, the suitability of installing facilities for 12 different sports was evaluated for potential contributions to the health and welfare of local residents. The utilization of abandoned mine spaces as data centers and public sports facilities is expected to not only recycle industrial heritage but also to allow new development opportunities for local communities.