• Title/Summary/Keyword: recycle fed-batch culture

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Enhanced production in recycle fed-batch cultivation by Lactic acid bacteria Isolated from Kimchi

  • Joe, Lim;Kwun, Kyu-Hyuk;Chang, Hae-Choon;Lee, Jung-Heon
    • 한국생물공학회:학술대회논문집
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    • 2005.10a
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    • pp.312-315
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    • 2005
  • A process for efficient recycle fed-batch culture was carried out to increase cell mass and spore production by Lactic acid bacteria isolated from Kimchi. A large quantity of cell mass obtained by feeding concentration of sugar in recycle fed-batch culture. When the high density of salt was created that the cell mass was come-down. In this study, cultured in different feeding concentration of sugar conditions. Lactic acid bacteria by recycle fed-batch culture was investigated in 2L working volume of fermenter, obtained the maximum cell mass was 15.17g/L.

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Production of Emulsan by Acinetobacter calcoaceticus RAG-1 under Various Culture Modes (여러 배양방법하에서 Acinetobacter calcoaceticus RAG-1에 의한 Emulsan의 생산)

  • 강병철;이필경장호남
    • KSBB Journal
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    • v.6 no.4
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    • pp.389-394
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    • 1991
  • Emulsan is an extracellular emulsifying agent produced by the hydrocarbon-degrading Acinetobacter species RAG-1. In this study emulsan production of Acinetobacter calcpaceticus RAG-1 was investigated under various culture modes such as batch, fed-batch, membrane cell recycle, and continuous culture. The productions of emulsan under both ethanol-sufficient fed-batch and membrane cell recycle cultures were all 15.0U/ml, which was 53% increase in emulsan activity compared to that of pH controlled batch culture. Emulsan production was found to be strongly dependent on the residual ethanol concentration. In continuous culture the emulsan productivity increased with dilution rate.

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