• Korean Journal of Environmental Biology
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• v.37 no.2
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• pp.129-135
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• 2019

Juvenile Paralichthys olivaceus (mean weight 685.3±36.7 g) were raised in different and closed recirculating bio-floc system (control, bio-ball, and shelter) for 7 months. The water environment such as water temperature, dissolved oxygen, salinity, pH, and alkalinity according to the difference of closed recirculating system remained stable during the rearing period. No significant changes were observed in dissolved inorganic nitrogen such as ammonia, nitrite, and nitrate were observed in different closed recirculation system. The final weights according to the difference of closed recirculating were 1,524 g (control), 1,674 g (bio-ball), and 1,630 g (shelter). The survival rate was higher than 98%, and the final FCRs (Feed coefficient ratio) were 1.2, 1.1, and 1.2. The results of this study indicated high growth and survival rate in all systems.

• ALGAE
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• v.29 no.1
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• pp.35-45
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• 2014

Palmaria palmata was integrated with Atlantic halibut Hippoglossus hippoglossus on a commercial farm for one year starting in November, with a temperature range of 0.4 to $19.1^{\circ}C$. The seaweed was grown in nine plastic mesh cages (each $1.25m^3$ volume) suspended in a concrete sump tank ($46m^3$) in each of three recirculating systems. Two tanks received effluent water from tanks stocked with halibut, and the third received ambient seawater serving as a control. Thalli were tumbled by continuous aeration, and held under a constant photoperiod of 16 : 8 (L : D). Palmaria stocking density was $2.95kg\;m^{-3}$ initially, increasing to $9.85kg\;m^{-3}$ after a year. Specific growth rate was highest from April to June (8.0 to $9.0^{\circ}C$), 1.1% $d^{-1}$ in the halibut effluent and 0.8% $d^{-1}$ in the control, but declined to zero or less than zero above $14^{\circ}C$. Total tissue nitrogen of Palmaria in effluent water was 4.2 to 4.4% DW from January to October, whereas tissue N in the control system declined to 3.0-3.6% DW from April to October. Tissue carbon was independent of seawater source at 39.9% DW. Estimated tank space required by Palmaria for 50% removal of the nitrogen excreted by 100 t of halibut during winter is about 29,000 to $38,000m^2$, ten times the area required for halibut culture. Fifty percent removal of carbon from the same system requires 7,200 to $9,800m^2$ cultivation area. Integration of P. palmata with Atlantic halibut is feasible below $10^{\circ}C$, but is impractical during summer months due to disintegration of thalli associated with reproductive maturation.

• Journal of Aquaculture
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• v.12 no.1
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• pp.47-56
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• 1999

Denitrification by anaerobic bacteria is one of the most common processes of removing nitrate from recirculating aquaculture systems. This process is affected by many factors such as external carbon sources, hydraulic retention time (HRT), and $COD/NO_3-N$ ratio. Although external organic carbon sources are essential for the denitrification process, these also contribute to increase dissolved organic carbon concentration in recirculating aquaculture systems. So these external organic carbons must be removed from the systems. This study was conducted to find out the optimum operating conditions for the removal of external organic carbons in a submerged denitrification biofilter. Combinations of two external carbon sources (glucose and methanol), two HRT (4- and 8-hour), and four different C:N ratios (3, 4, 5, and 6) were used in this experiment. The removal efficiencies of organic carbon sources at 8-hour HRT were always better than those at 4-hour's (P<0.05). Maximum removal efficiencies were achieved when C:N ratio was 5 in both glucose and methanol. The removal efficiencies of methanol were always better than those of glucose. The maximum removal efficiencies of glucose and methanol were 76.5% and 84.0%, respectively and the removal rates were 223.5 $g/m^2/day$ and 247.1$g/m^2/day$. The maximum removal rates of glucose (290.9 $g/m^2/day$) and methanol (355.6 $g/m^2/day$) were achieved at 4-hour HRT and 5 C:N ratio. But the concentrations of SCOD in the effluent of both glucose ($52.5 mg/\ell$) and methanol ($40.9 mg/\ell$) were too high for rearing fish. Therefore, the optimum operating conditions for the removal of external carbon in a submerged denitrification biofilter were 8-hour HRT and 5 C:N ratio. And methanol showed better efficiency as an external carbon sources.

• Journal of Aquaculture
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• v.10 no.3
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• pp.335-346
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• 1997

Denitrification is one of the important processes of removing nitrate from in recirculating aquaculture systems. And this process is affected by many factors such as external organic carbon sources, hydraulic retention time (HRT), COD/NO3--N (C:N) ratio, etc. However, not many studies were done for the optimum conditions of denitrification in the recirculation system for aquaculture. Therefore, this study was conducted to find out the optimum removal condition of NO3--N using submerged denitrification biofilter. The combinations of two external organic carbon sources (glucose and methanol), two HRT (4 and 8-hour) and four differnent C : N ratios (3, 4, 5, 6) were tested. The removal efficiencies of NO3--N and total inorganic nitrogen (TIM) at 8-hour HRT were better than those at 4-hour's (P<0.05). The maximum removal efficiency of NO3--N by methanol (97.8%) was achieved at HRT and C : N ratio were 8-hour and 4.0 respectively. The efficiencies of methanol for the removal of NO3--N and TIN were always better than those of glucose (P<0.05). The maximum removal efficiencies of total inorgainc nitrogen (TIN) were gained at C : N ration of 5.0. The maximum removel efficiencies of TIN using methanol and glucose were 96.9% and 71.5% respectively. Anaerobic condition which is necessary for denitrification process was not made until the 8-hour HRT and higher C : N ratio (5.0). Removal of NO3--N at 4-hour HRT and C : N ration lower than 5.0 were inhibited by oxygen and/or low quantity of external organic carbon. Removal efficiencies of NO3--N were also inhibited by high C : N (6.0) ratio when HRT was 8-hour.

• Journal of Aquaculture
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• v.18 no.2
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• pp.122-129
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• 2005

Ammonia is the major limiting factor in intensive aquaculture production systems. Therefore, quantification of ammonia excretion is important for the water quality management in aquaculture systems. Ammonia excretion is known to be affected by many factors such as body weight and dietary protein level (DPL). In this study, experiments were carried out to investigate the effects of body weight and DPLs on the rates of ammonia excretion of Nile tilapia Oreochromis niloticus. Three sizes of fishes (mean initial weight; 4.8 g,42.7 g and 176.8 g) were fed each of two dietary protein levels (30.5% and 35.5%). Daily feeding levels for the three fish sizes of 4.8 g, 42.7 g and 176.8 g were 6%, 3%, and 1.5% body weight per day, respectively. Each group of fish was stocked in a 17.1-L aquarium and all treatments were triplicated. Following feeding, the weight-specific ammonia excretion rate of O. niloticus increased, peaked at 4 to 8 h, and returned to pre-feeding levels within 24 h. Total ammonia nitrogen (TAN) excretion.ate per unit weight decreased with the increase of fish weight for each diet (P<0.05). The TAN excretion rate increased with increasing dietary protein content for each fish size (P<0.05). TAN excretion rates (Y) for each diet with different fish weights were described by the following equations: low DPL diet (30.5%): $Y\;(mg\;kg^{-1}\;d^{-1})=955.69-147.12\;lnX\;(r^2=0.95)$, high DPL diet (35.5%): $Y\;(mg\;kg^{-1}\;d^{-1})=1362.41-209.79\;lnX\;(r^2=0.99)$. Where: X=body weight (g wet wt.). The TAN excretion rates ranged 28.5%-37.1% of the total nitrogen ingested for the low DPL diet (30.5%) and 37.4-38.5% for the high DPL diet (35.5%). Total nitrogen losses of fish fed the high DPL diet $(35.5%;\;0.26\sim0.91g\;kg^{-1}\;d^{-1})$ were higher than those fed the low DPL diet $(30.5%;\;0.22\sim0.68g\;kg^{-1}\;d^{-1})$. The losses decreased per kg of fish as fish size increased. Results will provide valuable information fer water quality management and culture of Nile tilapia in recirculating aquaculture systems.

• Journal of Aquaculture
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• v.8 no.2
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• pp.117-131
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• 1995

In order to improve the present running seawater system for seedling production of marine fishes, rearing experiments with the juveniles of black seabream (Acanthopagrus schlegeli) and oblong rockfish (Sebastes oblongus) were conducted. The recirculating seawater system (F) equipped with the rotating biological contactors and the running seawater system (R) were used. Environmental factors, growth, survival rate and rearing density of the fish were compared between two systems during the experimental period. In the rearing experiment of black seabream, water temperature in F was fluctuated with surrounding air temperature and was higher than that in R. Specific gravity of the rearing seawater in F was relatively stable in the range of 1.0252 to 1.0266, while that in R was greatly fluctuated. pH in F turned out to be 7.51, but pH in R was similar to that in the natural seawater. Dissolved nitrite and nitrate in F were higher than those in R. While no significant differences in the growth of black seabream juveniles were recognized between two systems, survival rates of fishes in F were higher than those of fishes in R. In the rearing experiment of oblong rockfish, water temperature in F was higher than that in R. Specific gravity in F was slightly higher than that in R which showed relatively less fluctuation in the range of 1.0253 to 1.0270. pH in F turned out to be 7.96, but pH in R was similar to that in the natural seawater. Dissolved nitrite and nitrate in F were higher than those in R, but ammonia was lower in F. The juveniles of oblong rockfish reared in F grew significantly faster in their total length and body weight than those in R (P<0.05). However, survival rates of fishes between two systems showed no significant differences.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.6
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• pp.870-877
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• 2023

The purpose of this study was to assess the efficiency of nitrification based on ammonia loading rates and hydraulic air-loading rates in a moving bed bioreactor (MBBR) under seawater conditions. The goal was to provide foundational data for the design of these bio reactors. At an ammonia loading rate of 0.2 g TAN·m^-2 surface area·day^-1, the influent TAN concentration was determined to be 1.76±0.33 mg·L^-1, which is below the safe concentration for fish survival (2 mg·L^-1). Considering operational aspects, the optimal ammonia-loading rate was derived. Subsequently, experimental results for nitrification efficiency at the optimal ammonia-loading rate revealed that the optimum hydraulic air-loading rate was 1.8 L·air·m^-2 surface area·min^-1. This condition resulted in the lowest concentrations of TAN and NO₂-N in the influent water, thus establishing the optimal hydraulic air-loading rate. A regression equation was derived for the ammonia-removal rate (Y) based on the ammonia-loading rate (x) and expressed as a 0.5-order equation (Y=ax^0.5+b). Specifically, for TAN concentrations of 0-6 mg·L-1, the regression equation Y=0.1683x^0.5-0.13628, was established.

• Journal of fish pathology
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• v.37 no.1
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• pp.97-110
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• 2024

The Serranidae is high-quality fish species with good meat quality and is traded at high price, and is attracting attention in South Korea as a cultured species that creates high added value. However, the high-density fish farming for mass production increases the risk of mass mortality due to infectious diseases, leading to enormous economic losses. Therefore, in order to safely prevent and protect farmed fish from serious infectious diseases, it is necessary to conduct disease monitoring on a regular basis. In this study, Hyporthodus septemfasciatus, Epinephelus moara, and the hybrid longtooth grouper (E. moara ♀×E. lanceolatus ♂) were collected once a month from fish farm of Garorim and Aquabiotech Co., Ltd for a total of six months, from July to December 2023. We investigated infections of five species of bacterial diseases, including Flavobacterium columnare, six species of viral diseases, including LCDV (lymphocystis disease virus), and parasitic pathogens in grouper farms. As the result, Vibrio vulnificus and V. harveyi were detected in H. septemfasciatus in August, in the case of viral diseases, NNV was detected in H. septemfasciatus from July to August using RT-PCR or PCR. Finally, In the case of parasitic diseases, Tricodina sp. was detected in E. moara and the hybrid longtooth grouper from August to December.