• Natural Product Sciences
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• v.8 no.4
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• pp.152-154
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• 2002

The methanol extract of Viscum album var. coloratum, Korean mistletoe, showed potent prolongation effects on the bleeding time in rats in vivo, and whole blood clotting time and plasma recalcification time in rats ex vivo. The prolongation effect on the bleeding time of Korean mistletoe is comparable to that of Viscum album L., European mistletoe, 185.6% and 176.5%, respectively. However, the water extracts of the both plants did not show any prolongation effects. Platelet activating factor (PAF) receptor binding assay was carried out to elucidate the action mechanisms of the extracts, and both of the methanol extracts did not show any inhibitory activity. The $LD_{50}$ of the methanol extracts of both mistletoes are more than 2 g/kg. These results suggest that the mehtanol extract of Korean mistletoe might be a potential candidate to develop new drug to improve microcirculation.

• Archives of Plastic Surgery
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• v.38 no.2
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• pp.194-198
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• 2011

Purpose: Heterotopic calcification is the abnormal deposition of calcium salts in tissues other than bone and enamel, and it occurs in the form of dystrophic calcification or metastatic calcification. This deposition can occur under many conditions, but in some rare cases, it may develop in burns and nonhealed scars. It is difficult to treat the combination of heterotopic calcification and ulceration in scar tissues by using conservative therapy and to determine the margin of excision in such cases. Our study proposes the use of intraoperative C-arm-guided mapping of lesions with heterotopic calcification, and adequate excision of ulcers in chronic scars where heterotopic calcification is also observed. Methods: This study included 2 patients and was conducted from January 2010 to July 2010. The first patient was a 63-year-old woman who presented with atypical calcium deposits and chronic ulceration in the lower one-third region of the right leg. The second patient was a 38-year-old man who presented with a nonhealing ulcer that had developed on the right leg 3 months earlier he had a history of 40% scalding burns on the entire body. Surgery is the most reliable method for treating heterotopic calcification therefore, both patients were treated using intraoperative C-arm-guided marginal mapping of heterotopic calcification, followed by release of contracture, and eventually split-thickness skin grafting. Results: Plain radiographs of the leg showed spotty radiopaque areas in the hard part of the scar well superficial to the underlying bones. Histopathological analysis revealed multiple foci of calcified deposits, increased fibrosis, and inflammation in the scar tissue. Surgery-related complications were not observed. Conclusion: C-arm guided excision of calcified scars and the release of contracture can cure nonhealing ulcers and may therefore prevent recalcification.

• Applied Biological Chemistry
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• v.39 no.2
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• pp.159-164
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• 1996

Screening of anticoagulant activity was conducted for the hot water extracts of 73 kinds of medicinal herbs, 41 kinds of Korean edible plants, and 5 kinds of sea weeds using plasma recalcification test(Tr). In the first screening several extracts of the plants, Alisma calndiculatum, Corydalis ternata Panax notoginseng, Allium sativum, Ganderma luidum, Codium fragile, showed high activities. When the plants were reextracted with various solvent conditions, acidic water extracts of Codium fragile showed the highest activity in APTT. A crude polysaccharide fraction(CF-1) was prepared by methanol reflux, ethanol precipitation, dialysis and Iyophilization of the acid extracts. CF-1 comprised 80.8% total sugar consisting of arabinose, galactose and glucose as the main monomers, 8.7% protein, and 13.3% sulfate. The anticoagulant activity of CF-1 was not changed by pronase digestion, but decreased by periodate oxidation, and this indicated that the anticoagulant activity was attributed to the polysaccharide portion.

• Journal of the Korean Chemical Society
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• v.36 no.6
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• pp.947-954
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• 1992

Polyethylene glycol (PEG) grafted poly (γ-benzyl L-glutamate) (PBLG) was synthesized by the substitution reaction of PBLG and PEG having primary amino groups at both ends. PEG-g-PBLG films containing hydroxyl group were also prepared by the substitution reaction of PEG-g-PBLG film and ethanolamine (EA). Adhesion of platelets and activation of plasma proteins on the copolypeptide films were studied. The results showed that platelets are less adhered and activated on the PEG-g-PBLG than on other polypeptides and plasma recalcification time (PRT) on the PEG-g-PBLG was longer than that on other polypeptides. These results were consistent with those of blood clotting time and thrombus formation on the polypeptides. As a results, PEG-g-PBLG surfaces showed better blood compatibility than PBLG or PEG-g-PBLG-EA surfaces.

• Journal of Korean society of Dental Hygiene
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• v.11 no.1
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• pp.59-68
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• 2011

Objectives : To investigate the knowledge and recognition level of fluorine by teaching career among class teachers, who are primarily responsible for oral health education, despite lack of expertise in oral health, by playing a model role for students through continuous contacts with them, and provide basic elementary data about accurate knowledge and how to correctly use fluorine remarkably effective in preventing dental caries. Methods : A self-administered survey was conducted with 539 teachers at 21 elementary schools in Jinhae City from September 1 to October 15, 2010, drawing the following conclusions. Results : 1. As for the channel for acquiring knowledge of oral health, the highest frequency was found in health programs for respondents with less than 9 years of career, in dental clinics for those with 20 to 29 years of career, and in PR booklets for those with 10 to 19 years and 30 years and longer of career. 2. Use of fluorine-containing toothpaste and fluorine spread is very effective and effective in preventing dental caries got 64.9%, 72.2%, 72.3%, 77.7% and 54.0%, 63.1%, 62.2%, 69.7%, respectively, among elementary school teachers with less than 9 years, 10 to 19 years, 20 to 29 years, and 30 years and longer of career. 3. Intake of fluoride and toothbrushing with fluoric solution is very effective and effective in preventing dental caries got 33.3%, 40.1%, 39.5%, 55.3% and 50.0%, 54.9%, 48.8%, 69.7%, respectively, among elementary school teachers with less than 9 years, 10 to 19 years, 20 to 29 years, and 30 years and longer of career. 4. Use of fluoric tablets is very effective and effective in preventing dental caries got 32.9%, 36.0%, 36.1%, 47.3%, respectively, among elementary school teachers with less than 9 years, 10 to 19 years, 20 to 29 years, and 30 years and longer of career. 5. Most of the elementary school teachers knew the fact that fluorine increases dental resistance to cavity and prevents bacterial growth but gave incorrect responses to properties of becoming basic nutrients or inducing recalcification. Conclusions : Various types of application of fluorine during the elementary-level childhood experiencing the most frequent dental caries will be very useful in improving oral health in the future. It is necessary to enable class teachers to get correct recognition of fluorine through supplementary and job training based on technical knowledge and various educational materials so that they, who are directly responsible for health management through continuous contacts with students, can understand how to correctly use fluorine and get accurate knowledge.

• Journal of dental hygiene science
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• v.13 no.4
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• pp.461-470
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• 2013

In order to examine the anticariogenic effect after fluoride varnish was applied to sound enamel and artificial caries enamel, anterior teeth of healthy cattle were used and divided into four groups such as group 1 (sound enamel), group 2 (application of fluoride varnish to sound enamel), group 3 (artificial caries enamel) and group 4 (application of fluoride varnish to artificial caries enamel). Remineralization on the surface of enamel and changes of crystalline structure after demineralization were observed by using a field emission scanning electron microscope (FE-SEM). Quantitative analysis of Ca and P was measured by using the energy dispersive X-ray spectrophotometer (EDS). The following conclusions were obtained: 1)Surface pattern of enamel was the roughest in group 3 due to the defects caused by porosity and microcracks. Group 4, group 1 and group 2 were followed in order; 2) It was found that pattern of crystalline structures in a group of application of fluoride varnish and a group of no application showed bigger change in artificial caries enamel than that in sound enamel. In other words, groups 4 and 1 showed a similar pattern; 3) The contents of Ca and P were higher in groups of application of fluoride varnish (group 2 and group 4) than in groups of no application of fluoride varnish (group 1 and group 3). Taken results of this study together, in the case of application of fluoride varnish, crystalline structure was changed by remineralization even in the sound enamel. In particular, porous structures showed a smooth and uniform pattern due to the recalcification in the artificial caries enamel. In addition, according to results of EDS analysis, the contents of Ca and P were increased and it had great anticariogenic effects which inhibit decalcification of sound enamel and artificial caries enamel.