• Korean Journal of Ecology and Environment
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• v.52 no.4
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• pp.394-402
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• 2019

The Agrobacterium tumefaciens mediated gene transfer is widely used to generate genetic transformation of plants and transient assay of temporal exogenous gene expression. Syringe infiltration system into tobacco (Nicotiana benthamiana) leaves is a powerful tool for transient expression of target protein to study protein localization, protein-protein binding and protein production. However, the protocol and technical information of transient gene expression, especially double strand RNA (dsRNA), in tobacco using Agrobacterium is not well known. Recently, dsRNA is crucial for insecticidal effect on destructive agronomic pest such as Corn rootworm. In this study, we investigated the factor influencing the dsRNA expression efficiency of syringe agro-infiltration in tobacco. To search the best combination for dsRNA transient expression in tobacco, applied two Agrobacterium cell lines and three plant vector systems. The efficiency of dsRNA expression has estimated by real-time PCR and digital PCR. As a result, pHellsgate12 vector constructs showed the most effective accumulation of dsRNA in the cell. These results indicated that the efficiency of dsRNA expression was depending on the kind of vector rather than Agrobacterium cells. In summary, the optimized combination of transient dsRNA expression system in tobacco might be useful to in vivo dsRNA expression for functional study and risk assessment of dsRNA.

• Journal of Animal Science and Technology
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• v.50 no.1
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• pp.45-56
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• 2008

In the present study, real-time PCR was performed to evaluated expression of several isoforms of monocarboxylate transporters(MCTs) and two known MCT regulatory proteins, basigin (Bsg) and embigin, in the epididymis of the male reproductive tract during postnatal development. In addition, ERα�-mediated regulation of MCT1 expression in the epididymis was determined with estrogen receptor(ER) α� knockout(α�ERKO) mice by immunohistochemistry. Results from the current study demonstrated differential expression of MCT isoform(MCT 1, 2, 3, 4, and 8), Bsg, and embigin mRNAs in rat epididymis according to postnatal age and epididymal region. In addition, immunohistochemical study of MCT1 revealed the limited localization of MCT1 at apical area of corpus and caudal epididymis. The present study also showed that expression of MCT1 was not directly regulated by ERα�. The findings from the current study suggest that MCTs would involve in establishing adequate microenvironment for sperm maturation and storage in the epididymis, eventually leading to maintenance of male fertility.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.12A
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• pp.1238-1245
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• 2006

Interest to the RTLS that is one of RFID applications is increasing in recent. The RTLS(Real Time Locating Systems) is one of applications for locating and tracking using RFID tags which are attached to something like container, pallet, or all the things. This paper presents the design and the implementation of an RTLS system using 433MHz active RFID tags and use radio frequency to provide the scalability. Our system we developed using RFID platform takes into account an RTLS standard. Also, in this paper a routing protocol is included to data delivery to server via each reader. In order to perform the evaluation, in addition, some experiments in out door are performed and results such as error metric and distance are also included. Furthermore, simulation for the routing protocol we supposed is also included.

• Proceedings of the KIEE Conference
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• 2006.05a
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• pp.74-77
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• 2006

This research is focused on the development of GIS Diagnostic SYSTEM to prevent accidents beforehand by inspecting the internal defects of GIS (Gas Insulated Switchgear) which is a main power-facility for substations. GIS Diagnostic SYSTEM is categorized as 'Real time on-line test type' and 'Portable test type' depending on the types of testing, it uses PD(partial discharge) which mostly incurs in GIS internal defects, to inspect. As of now, mostly foreign equipments are imported for use due to the lack of the technology localization, and these are installed and operated on only some parts of highly-graded GIS power-facilities such as in 76skv or 345kv for its being expensive. Furthermore, other than foreign equipments being costly, it also has a weak point of very long unavailability in case of Diagnostic system break-down while using, because it takes a comparatively long period of corrective maintenance precesses. We have localized to develop personal real-time multi -functional GIS Diagnostic system which can test on all GIS power-facility comprehensively and economically therefore overcome all these problems mentioned above, a market expansion is expected from the decrease of price and replacing the import equipments in the future. As the equipment was developed to be Personal for the simple ways of installing and utilizing, it can be operated without any complex cable installation like other existing GIS Diagnostic system requires, therefore also decrease the cost of cable installation.

• The korean journal of orthodontics
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• v.44 no.6
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• pp.320-329
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• 2014

Objective: To investigate the involvement of ephrinB2 in periodontal tissue remodeling in compression areas during orthodontic tooth movement and the effects of compressive force on EphB4 and ephrinB2 expression in osteoblasts and osteoclasts. Methods: A rat model of experimental tooth movement was established to examine the histological changes and the localization of ephrinB2 in compressed periodontal tissues during experimental tooth movement. RAW264.7 cells and ST2 cells, used as precursor cells of osteoclasts and osteoblasts, respectively, were subjected to compressive force in vitro. The gene expression of EphB4 and ephrinB2, as well as bone-associated factors including Runx2, Sp7, NFATc1, and calcitonin receptor, were examined by quantitative real-time polymerase chain reaction (PCR). Results: Histological examination of the compression areas of alveolar bone from experimental rats showed that osteoclastogenic activities were promoted while osteogenic activities were inhibited. Immunohistochemistry revealed that ephrinB2 was strongly expressed in osteoclasts in these areas. Quantitative real-time PCR showed that mRNA levels of NFATc1, calcitonin receptor, and ephrinB2 were increased significantly in compressed RAW264.7 cells, and the expression of ephrinB2, EphB4, Sp7, and Runx2 was decreased significantly in compressed ST2 cells. Conclusions: Our results indicate that compressive force can regulate EphB4 and ephrinB2 expression in osteoblasts and osteoclasts, which might contribute to alveolar bone resorption in compression areas during orthodontic tooth movement.

• Journal of the Institute of Electronics and Information Engineers
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• v.52 no.9
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• pp.89-95
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• 2015

In this paper, we propose a real-time two-dimensional bar code detection system even at long range using a vision technique. We first perform short-range detection, and then long-range detection if the short-range detection is not successful. First, edge map generation, image binarization, and connect component labeling (CCL) are performed in order to select a region of interest (ROI). After interpolating the selected ROI using bilinear interpolation, a location symbol pattern is detected as the same as for short-range detection. Finally, the symbol pattern is arranged by applying inverse perspective transformation to localize bar codes. Experimental results demonstrate that the proposed system successfully detects bar codes at two or three times longer distance than existing ones even at indoor environment.

• Journal of Korea Multimedia Society
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• v.8 no.8
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• pp.1080-1087
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• 2005

Significant computations for full search (FS) motion estimation have been a big obstacle in real-time video coding and recent MPEG-4 AVC (advanced video coding) standard requires much more computations than conventional MPEG-2 for motion estimation. To reduce an amount of computation of full search (FS) algorithm for fast motion estimation, we propose a new and fast matching algorithm without any degradation of predicted images like the conventional FS. The computational reduction without any degradation in predicted image comes from fast elimination of impossible candidate motion vectors. We obtain faster elimination of inappropriate motion vectors using efficient matching units from localization of complex area in image data and dithering order based matching scan. Our algorithm reduces about $30\%$ of computations for block matching error compared with the conventional partial distortion elimination (PDE) algorithm, and our algorithm will be useful in real-time video coding applications using MPEG-4 AVC or MPEG-2.

• Korean Journal of Agricultural Science
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• v.47 no.2
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• pp.315-326
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• 2020

In this study, a real-time crop recognition system was developed for an unmanned farm machine for upland farming. The crop recognition system was developed based on a stereo camera, and an image processing framework was proposed that consists of disparity matching, localization of crop area, and estimation of crop height with coordinate transformations. The performance was evaluated by attaching the crop recognition system to a tractor for five representative crops (cabbage, potato, sesame, radish, and soybean). The test condition was set at 3 levels of distances to the crop (100, 150, and 200 cm) and 5 levels of camera height (42, 44, 46, 48, and 50 cm). The mean relative error (MRE) was used to compare the height between the measured and estimated results. As a result, the MRE of Chinese cabbage was the lowest at 1.70%, and the MRE of soybean was the highest at 4.97%. It is considered that the MRE of the crop which has more similar distribution lower. the results showed that all crop height was estimated with less than 5% MRE. The developed crop recognition system can be applied to various agricultural machinery which enhances the accuracy of crop detection and its performance in various illumination conditions.

• Nuclear Engineering and Technology
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• v.52 no.10
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• pp.2250-2261
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• 2020

Accurate localization of radioactive materials is crucial in homeland security and radiological emergencies. Coded-aperture gamma camera is an interesting solution for such applications and can be developed into portable real-time imaging devices. However, traditional reconstruction methods cannot effectively deal with signal-independent noise, thereby hindering low-noise real-time imaging. In this study, a novel reconstruction method with excellent noise-suppression capability based on a multi-layer perceptron (MLP) is proposed. A coded-aperture gamma camera based on pixel detector and coded-aperture mask was constructed, and the process of radioactive source imaging was simulated. Results showed that the MLP method performs better in noise suppression than the traditional correlation analysis method. When the Co-57 source with an activity of 1 MBq was at 289 different positions within the field of view which correspond to 289 different pixels in the reconstructed image, the average contrast-to-noise ratio (CNR) obtained by the MLP method was 21.82, whereas that obtained by the correlation analysis method was 5.85. The variance in CNR of the MLP method is larger than that of correlation analysis, which means the MLP method has some instability in certain conditions.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.33 no.1
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• pp.1-6
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• 2022

Vocal fold injection (VFI) is widely accepted as a first line treatment in treating unilateral vocal fold paralysis and other vocal fold diseases. Although VFI is advantageous for its minimal invasiveness and efficiency, the invisibility of the needle tip remains an essential handicap in precise localization. Real-time light-guided vocal fold injection (RL-VFI) is a novel technique that was developed under the concept of performing simultaneous injection with precise placement of the needle tip under light guidance. RL-VFI has confirmed its possibility of technical implementation and the feasibility in injecting the needle from various directions through ex vivo animal studies. Further in vivo animal study has approved the safety and feasibility of the procedure when various transcutaneous approaches were applied. Currently, RL-VFI device is authorized for clinical use by the Ministry of Food and Drug Safety in South Korea and is clinically applied to patients with safe and favorable outcome. Several clinical studies are currently under process to approve the safety and the efficiency of RL-VFI. RL-VFI is expected to improve the complication rate and the functional outcome of voice. Furthermore, it will support laryngologists in overcoming the steep learning curve by its intuitive guidance.