• Title/Summary/Keyword: reaction degree

Search Result 1,095, Processing Time 0.048 seconds

Antibacterial Activity of Lysozyme-Galactomannan Conjugate against Escherichia coli

  • Hwang, Jae-Kwan;Kim, Hyun-Jin;Park, Moon-Jung;Shin, Hae-Hun;Pyun, Yu-Ryang
    • Preventive Nutrition and Food Science
    • /
    • v.3 no.4
    • /
    • pp.320-323
    • /
    • 1998
  • Lysozyme was covalentyl conjugated with galactomannan through a amino-carbonyl reaction between the lysine $\varepsilon$-amino groups of lysozyme and the reducing ends of galactomannan at a relative humidity of 79% and 6$0^{\circ}C$. The resulting lysozyme-galactomannan conjugate (LGC) was investigated for its antibacterial activity against Escherichia coli. Lysozyme alone did not exhibit antibacterial activity against E. coli. in contrast , significant bactericidal effect was observed for LGC, depending on the reaction temperature. The degree of conjugation between lysozyme and galactomannan was dependent on the incubation time, which affected the antibacterial efficiency against E. coli. This study demonstrated that the amino-carbonyl reaction between lysozyme and galactomannan could be a potential tool to modify lysozyme toward broadening its antibacterial spectrum to Gram-negative bacteria.

  • PDF

Prediction of temperature distribution in hardening silica fume-blended concrete

  • Wang, Xiao-Yong
    • Computers and Concrete
    • /
    • v.13 no.1
    • /
    • pp.97-115
    • /
    • 2014
  • Silica fume is a by-product of induction arc furnaces and has long been used as a mineral admixture to produce high-strength, high-performance concrete. Due to the pozzolanic reaction between calcium hydroxide and silica fume, compared with that of Portland cement, the hydration of concrete containing silica fume is much more complex. In this paper, by considering the production of calcium hydroxide in cement hydration and its consumption in the pozzolanic reaction, a numerical model is proposed to simulate the hydration of concrete containing silica fume. The heat evolution rate of silica fume concrete is determined from the contribution of cement hydration and the pozzolanic reaction. Furthermore, the temperature distribution and temperature history in hardening blended concrete are evaluated based on the degree of hydration of the cement and the mineral admixtures. The proposed model is verified through experimental data on concrete with different water-to-cement ratios and mineral admixture substitution ratios.

Preparation of Blood Glue from Porcine Plasma Protein and Cross-linking Reaction of Plasma Protein with Formaldehyde

  • Cho, Yongsik;Lee, Hwahyoung;Song, Kyung Bin
    • Journal of Applied Biological Chemistry
    • /
    • v.42 no.2
    • /
    • pp.81-84
    • /
    • 1999
  • Blood glue was prepared to reutilize porcine blood. Plasma proteins after lyophilization were treated by addition of wood flour, sodium hydroxide, sodium silicate, and hydrated lime to make blood glue with a suitable adhesivity. Characteristics of the prepared blood glue was monitored by measuring the viscosity with time, and the relationship between degree of hydrolysis of plasma proteins by addition of various amounts of sodium hydroxide and adhesivity was studied. To prevent the emission of formaldehyde during manufacturing of plywood by blood glue, the cross-linking reaction of plasma protein with formaldehyde was also examined. Fourier transform infrared, circular dichroism, and fluorescence spectroscopy study showed that blood plasma proteins react with formaldehyde, resulting in removal of formaldehyde by cross-linking reaction.

  • PDF

Stydies on the Substitution of the Activated Aromatic Chloride with Alkoxy Group. (방향족 활성 Chloro화합물의 Alkoxy기 치환에 관한 연구)

  • 조윤상;공영식
    • YAKHAK HOEJI
    • /
    • v.19 no.2
    • /
    • pp.101-110
    • /
    • 1975
  • p-Chloronitrobenzene(substrate) and p-nitrophenetole (product) were quantitatively analyzed to know the degree of extent of reaction in the process of time. The calibration curve was prepared by the internal satndard method in gaschromatography. 2,6-Dimethyl-naphthalene was used as internal standard. The rate constant(k), the reaction velocity in various concentrations of NaOH altered, and the formation of byproducts(azo-compound and p-nitrophenol) with the amounts of MnO$_{2}$ and NaOH altered, were studied. From the results of these of MnO$_{2}$ and NaOH altered, were studied. From the results of these experiments, this reaction was second order and the rate constant was k=10.3 $\times$ 10$^{-3}$ mole$^{-21$. When p-chloronitrobenaene 1 pt. NaOH 0.56pts. MnO$_{2}$ 0.5pts. and ethanol 25 pts-were reacted about 10 hours, p-nitrophenetole was nearly quantitatively obtained without byproducts.

  • PDF

Properties of Sol-Gel Thin Films Containing Colloidal Silica and Alkoxysilanes (콜로이드 실리카 알콕시실란을 함유한 졸겔반응 경화박막 특성연구)

  • Myung, In-Hye;Ahn, Myeong-Sang;Kang, Young-Taec;Kang, Dong-Pil
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
    • /
    • 2006.06a
    • /
    • pp.230-231
    • /
    • 2006
  • We synthesized sol according to kinds(particle size/stabilized ion) of colloidal silica(CS), content ratio of alkoxysilane versus CS and reaction degree in sol solution and studied the surface property of coated gel materials. The contact angle of the thin films prepared from LHSA/N1030 CS/tetramethoxysilane(TMOS)/methyltrimethoxysilane(MTMS) sol-gel reaction system showed a little good relationship with content ratio of TMOS/MTMS silanes. The surface roughness of LHSA CS/TMOS/MTMS reaction system showed flatter than that of LHSA/N1030 CS. The thermal degradation of LHSA CS/TMOS/MTMS coating flim occurred at $550^{\circ}C$.

  • PDF

Genotyping of HLA-DRB1 by Polymerase Chain Reaction-Sequence Specific Primer (Polymerase Chain Reaction-Sequence Specific Primer를 이용한 HLA-DRB1 유전자의 DNA 다형성)

  • Jang, Soon-Mo
    • Korean Journal of Clinical Laboratory Science
    • /
    • v.37 no.3
    • /
    • pp.139-142
    • /
    • 2005
  • Most expressed HLA(human leukocyte antigen) loci exhibit a remarkable degree of allelic polymorphism, which is derived from sequenceing differences predominantly localized to discrete hypervariable regions of the amino-terminal domain of the molecule. In this study, the HLA-DRB1 genotypes were determined in twenty students using the PCR-SSP (polymerase chain reaction-sequence specific primer) technique. Two specific primer pairs in assigning the DRB1 gene were used. The results of PCR-SSP, the $HLA-DRB1^{\ast}0101$ primer detected nine and $HLA-DRB1^{\ast}1501$ primer detected three people. This study shows that the PCR-SSP technique is relatively simple, fast and a practical tool for the determination of the HLA-DRB1 genotypes. Moreover, these genotype frequency results of the HLA DRB1 gene could be useful for database study before being applied to individual identification and transplantation immunity.

  • PDF

Transglycosylation Reaction on Cellobiose by Dextansucrase of Leuconostoc mesenteroides B512FMC/6HG8 (Leuconostoc mesenteroides B512FMC/6HG8가 생산하는 Dextransucrase에 의한 Cellobiose의 당전이반응)

  • 강현록;양지영;이현규
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.29 no.5
    • /
    • pp.802-806
    • /
    • 2000
  • The transglycosylation reaction by dextansucrase from Leuconostoc mesenteroides B512FMC/6HG8 was investigate with cellobiose as an acceptor molecule and sucrose as a donor. he optimal conditions of transglycosylation on cellobiose were found that the ration of sucrose to cellobiose was 3:1, the amount of enzyme was 2U/mL, the ionic strength of buffer was 25 mM, pH was 5.0 and reaction temperature was $25^{\circ}C$. also, acceptor products of cellobiose by transglycosylation were a series of oligosaccharides showing the degree of plymenzation of 6.

  • PDF

Crack-Free Fabrications of Yttria-Stabilized Zirconia Films Using Successive-Ionic-Layer-Adsorption-and-Reaction and Air-Spray Plus Method

  • Taeyoon Kim;Sangmoon Park
    • Korean Journal of Materials Research
    • /
    • v.34 no.2
    • /
    • pp.79-84
    • /
    • 2024
  • Thin films of yttria-stabilized zirconia (YSZ) nanoparticles were prepared using a low-temperature deposition and crystallization process involving successive ionic layer adsorption and reaction (SILAR) or SILAR-Air spray Plus (SILAR-A+) methods, coupled with hydrothermal (175 ℃) and furnace (500 ℃) post-annealing. The annealed YSZ films resulted in crystalline products, and their phases of monoclinic, tetragonal, and cubic were categorized through X-ray diffraction analysis. The morphologies of the as-prepared films, fabricated by SILAR and SILAR-A+ processes, including hydrothermal dehydration and annealing, were characterized by the degree of surface cracking using scanning electron microscopy images. Additionally, the thicknesses of the YSZ thin films were compared by removing diffusion layers such as spectator anions and water accumulated during the air spray plus process. Crack-free YSZ thin films were successfully fabricated on glass substrates using the SILAR-A+ method, followed by hydrothermal and furnace annealing, making them suitable for application in solid oxide fuel cells.

A Study on the Estimating the Degree of Reaction for a Turbine Using a Synchronizable Turbocharging System (동기화 터보 챠저계를 이용한 터빈 반동도 예측에 관한 연구)

  • 김창훈
    • Journal of the Korean Society of Fisheries and Ocean Technology
    • /
    • v.33 no.3
    • /
    • pp.234-240
    • /
    • 1997
  • 터보 챠저의 반동도와 터빈 노즐유량의 변화를 행하여 엔진의 성능을 향상시킬 수 있었다. 터빈의 에너지 손실과 그 영향을 미치는 반동도 및 터빈 입구의 유로의 변화, 그리고 블레이드에서 역 유동에 대한 개선 조건도 제시하였다.

  • PDF

Measurement of Degree of Hydrolysis of a PA66/GF Composite using a py-GC/MS analysis

  • Lee, Jong-Young;Kim, Kwang-Jea
    • Elastomers and Composites
    • /
    • v.52 no.1
    • /
    • pp.59-68
    • /
    • 2017
  • The effect on the hydrolysis resistance properties by the addition of maleic anhydride grafted EMDM (MA-g-EPDM) and PP (MA-g-PP) to a PA66/GF composite was investigated with respect to the mechanical properties, thermal properties, and morphology. The degree of hydrolysis of the PA66/GF composite was measured using py-GC/MS analysis. When compared to the PA66/GFcomposite in MEG/water solution, the composites where MA-g-EPDM and MA-g-PP were added to PA66/GF showed a higher degree of hydrolysis resistance, impact strength, and thermal properties, whereas their tensile strength, tensile modulus, flexural strength and flexural modulus decreased. As immersion time in the solution increases, the rate of tensile strength drop of the MA-g-PP added composite appeared lower than that of the PA66/MA-g-EPDM/GF and PA66/GF composites. The py-GC/MS analysis confirmed the formation of PA66 hydrolysis reaction by products such as carboxylic acid and alkylamine with increasing immersion time.