• 제목/요약/키워드: re-characterization

검색결과 101건 처리시간 0.027초

Genetic Diversity in the Coat Protein Genes of Prune dwarf virus Isolates from Sweet Cherry Growing in Turkey

  • Ozturk, Yusuf;Cevik, Bayram
    • The Plant Pathology Journal
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    • 제31권1호
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    • pp.41-49
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    • 2015
  • Sweet cherry is an important fruit crop with increasing economical value in Turkey and the world. A number of viruses cause diseases and economical losses in sweet cherry. Prune dwarf virus (PDV), is one of the most common viruses of stone fruits including sweet cherry in the world. In this study, PDV was detected from 316 of 521 sweet cherry samples collected from 142 orchards in 10 districts of Isparta province of Turkey by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). The presence of PDV in ELISA positive samples was confirmed in 37 isolates by reverse transcription- polymerase chain reaction (RT-PCR) method. A genomic region of 862 bp containing the coat protein (CP) gene of PDV was re-amplified from 21 selected isolates by RT-PCR. Amplified DNA fragments of these isolates were purified and sequenced for molecular characterization and determining genetic diversity of PDV. Sequence comparisons showed 84-99% to 81-100% sequence identity at nucleotide and amino acid level, respectively, of the CP genes of PDV isolates from Isparta and other parts of the world. Phylogenetic analyses of the CP genes of PDV isolates from different geographical origins and diverse hosts revealed that PDV isolates formed different phylogenetic groups. While isolates were not grouped solely based on their geographical origins or hosts, some association between phylogenetic groups and geographical origins or hosts were observed.

이중층 워시코트 Rh-Pd-Pt 삼원촉매의 열적 열하에 따른 반응 특성 (Characteristics of Rh- Pd- Pt Three-Way Catalysts with Double-Layer Washcoat on the Hydrothermal Aging)

  • 최병철;정종우;손건석;정명근
    • 한국자동차공학회논문집
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    • 제14권1호
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    • pp.8-16
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    • 2006
  • The research was conducted to characterize of Rh-Pd-Pt TWC with a double-layer washcoat for gasoline vehicle. The physical characteristics on surface of catalyst were inspected by BET, SEM and TEM. The characteristics of catalytic reaction were examined by the TPD/TPR and CO-pulse chemisorption. The catalyst $6Hx(0.35\times11\times3)$ showed superior conversion performance after hydrothermal aging process, which was due to small difference of the surface area between. the fresh and the aged catalyst. The CO-chemisorption and surface area were superior in the 600 cpsi catalyst than other catalysts, this catalyst also shown the higher conversion efficiency of the exhaust emissions. From the TPR test, the conversion performance of the aged catalyst was decreased by the agglomeration and sintering of the PM and metal oxides. From the TPD result, it was found that the NO chemisorption was happed on the bottom-layer washcoat with Pd, and the NO chemisorption was re-happened on the upper-layer washcoat with Pt and Rh in the desorption process.

Systematic investigation of heavy metals from MSWI fly ash and bottom ash samples

  • Ramakrishna., CH;Thriveni., T;Ahn, Ji Whan
    • 에너지공학
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    • 제26권4호
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    • pp.35-44
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    • 2017
  • Disposal of municipal solid waste has become a major problem in many countries around the world. As landfill space for the disposal of ash from Municipal Solid Waste Incineration (MSWI) becomes scarce, numerous reports and researches address the various environmental issues about the municipal solid waste incineration waste management and other particulate matters with the range of 10 ~ 2.5. Although in many developing and industrialization countries landfill with the disposal of municipal solid waste, open incineration has become a common practice. Large municipal waste incinerators are major industrial facilities and have the potential to be significant sources of environmental pollution. Despite the significant volume reduction from incineration, waste recycling is important to ensuring the future welfare of mankind. The main goal of the present work is the physical and chemical characterization of the local incineration bottom ash towards its eventual re-utilization. In this paper, we reported the studies on physical and chemical characteristics of municipal solid waste incineration (MSWI) fly ash and bottom ash containing particulate matter whose particulate sizes are lower than $PM_{10}$, $PM_{2.5}$ and heavy metal were investigated.

Characterization of Silver Saturated-Ge45Te55 Solid Electrolyte Films Incorporated by Nitrogen for Programmable Metallization Cell Memory Device

  • Lee, Soo-Jin;Yoon, Soon-Gil;Yoon, Sung-Min;Yu, Byoung-Gon
    • Transactions on Electrical and Electronic Materials
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    • 제8권2호
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    • pp.73-78
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    • 2007
  • The crystallization temperature in GeTe solid electrolyte films was improved by in situ-nitrogen doping by rf magnetron co-sputtering technique at room temperature. The crystallization temperature of $250\;^{\circ}C$ in electrolyte films without nitrogen doping increased by approximately $300\;^{\circ}C$, $350\;^{\circ}C$, and above $400\;^{\circ}C$ in films deposited with nitrogen/argon flow ratios of 10, 20, and 30 %, respectively. A PMC memory device with $Ge_{45}Te_{55}$ solid electrolytes deposited with nitrogen/argon flow ratios of 20 % shows reproducible memory switching characteristics based on resistive switching at threshold voltage of 1.2 V with high $R_{off}/R_{on}$ ratios. Nitrogen doping into the silver saturated GeTe electrolyte films improves the crystallization temperature of electrolyte films and does not appear to have a negative impact on the switching characteristics of PMC memory devices.

친환경 기능성 무기질계와 고무아스팔트를 혼합한 내진방수 보강공법에 대한 실험적 평가 (Experimental assessment for friendly-environment functional Inorganic mixed rubber asphalt Seismic waterproof strengthening method)

  • 백종명;황영호;손정철
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2008년도 춘계학술대회 논문집
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    • pp.1802-1808
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    • 2008
  • Recent interest in the construction sector, rising about the environment and eco-friendly recycled material resources, and increase the development of method But despite these efforts, and the diverse functional and structural changes in the structure can not be an appropriate response to the functional waterproof structural changes in the structures and appropriate response Diversification does not waterproof and functional issues such as durability, and which are occurring due to the structure to secure stability and durability, never sees the conservative economic losses due to import constructability reinforcement situation. Therefore, this study applies to structure the existing waterproof method (hereinafter referred to as structures water-resistant methode), and to review recent issues of environmental pollution and resource waste, and taking on environmental issues, such as Revelation and functional Inorganic mixed in a way to leverage the manufacturing water-resistant material "Re Inorganic, functional and environmentally friendly high-viscosity mix asphalt waterproof rubber reinforcements, and taking conservative" for the characterization and performance assessment to the issues raised by the structure and whether the judge would respond.

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Growth and Characterization of Graphene Controlled by Cooling Profile Using Near IR CVD

  • 박윤재;임영진;김진환;최현광;전민현
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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    • pp.207-207
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    • 2013
  • 기존의 그래핀 성장에 관한 연구는 열화학기상증착법(Chemical vapor deposition; CVD)을 이용한다. 그래핀 성장 제어 요소로는 촉매 기판인 전이 금속[Ru, Ir, Co, Re, Pt, Pd, Ni, Cu], 기판 전처리 과정, 수소/메탄 가스 혼합비, 작업 진공 상태, 기판온도[$800{\sim}1,000^{\circ}C$, 냉각 속도 등으로 보고 되고 있다. 그래핀 성장 원리는 Cu 촉매 기판에 메탄 가스를 $1,000^{\circ}C$ 온도에서 분해해서 탄소를 고용 시킨 후 급랭하는 도중에 석출되는 탄소에 의해 그래핀 시트가 형성되는 것으로 알려져 있다. 기존의 CVD를 열원을 이용할 경우 내부 챔버에 생기는 잠열에 의해 cooling profile의 제어가 용이하지 않다. 본 연구에서는 근적외선(Near Infrared; NIR) 열원을 이용한 CVD로 챔버 내부 잠열을 최소화하고, 냉각 공정을 Natural, Linear, Convex cooling type으로 디자인해서 cooling profile 제어가 그래핀 성장에 미치는 영향을 연구 하였다. 이렇게 성장된 그래핀을 임의의 기판(SiO2, Glass, PET film) 위에 습식방법으로 전이 시킨 후, 전기적 구조적 및 광학적 특성을 면저항(four-point probe), 전계방사 주사전자현미경(Field Emission Scanning Electron Microscope; FE-SEM), 마이크로 라만 분광법(Micro Raman spectroscopy) 및 광학현미경(optical microscope), 투과도(UV/Vis spectrometer)의 측정으로 잠열이 최소화된 NIR-CVD에서 cooling profile에 따른 그래핀 성장을 평가하였다.

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헬륨가스 냉각 고온초전도 케이블의 절연특성 평가 시스템 개발 및 성능평가 (Development and Test results of the Dielectric Evaluation System for a Helium Gas Cooled HTS Cable)

  • 곽동순
    • 한국초전도ㆍ저온공학회논문지
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    • 제14권1호
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    • pp.25-29
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    • 2012
  • The novel type of cable under consideration is cooled by gaseous Helium at elevated pressure. Helium is known for having poor electric breakdown strength; therefore the dielectric capabilities of this type of cable must be tested under conditions similar to the envisaged operation. In order to study the dielectric performance we have designed and built a novel high pressure cryostat rated at 2.17 MPa which has been used for testing model cables of lengths of up to 1 m. The cryostat is an open system where the gas is not re-circulated. This allows maintaining a high purity of the gas. The target temperature range is between 40 K and 70 K. This substantially increases the critical current density of the HTS compared to 77 K, which is the typical temperature of cables cooled by liquid nitrogen. The cryostat presented allows for adjusting the temperature and keeping it constant for the time necessary to run a complete dielectric characterization test. We give a detailed description of the cryostat. Measurements of partial discharge inception voltages as well as the temperature distribution along the model cables as a function of time are presented.

In-situ Characterization of Electrochemical and Frictional Behaviors During Copper CMP

  • 엄대홍;강영재;박진구
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2004년도 하계학술대회 논문집 Vol.5 No.1
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    • pp.227-230
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    • 2004
  • As the organic acids were added in the slurry, zeta potential of alumina was changed to negative value and IEP value was shifted from alkaline to acidic pH. In citric acid based slurry, Cu surface continuously dissolved and etching depth linearly increased. On the contrary, passivation layer was grown on Cu surface in oxalic acid based slurry. As the platen rotation speed increased, Preston coefficient decreased in both slurries. With oxalic acid based slurry, at low velocity, removal rate is high value because of high friction force compared to citric acid based slurry. As platen velocity increased, removal of Cu in citric acid based slurry became higher value than oxalic acid based slurry. Typical lubrication behaviors were observed in both slurries. As Sommerfeld number increased, COF values gradually decreased and then re-increased. It indicated that lubrication was changed to direct contact or semi-direct contact mode to hydro-lubrication mode.

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Characterization of Copper Saturated-$Ge_xTe_{1-x}$ Solid Electrolyte Films Incoperated by Nitrogen for Programmable Metalization Cell Memory Device

  • Lee, Soo-Jin;Yoon, Soon-Gil;Yoon, Sung-Min;Yu, Byoung-Gon
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2007년도 하계학술대회 논문집 Vol.8
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    • pp.174-175
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    • 2007
  • A programmable metallization cell (PMC) memory structure with copper-saturated GeTe solid electrolyte films doped by nitrogen was prepared on a TiW bottom electrode by a co-sputtering technique at room temperature. The $Ge_{45}Te_{55}$ solid electrolyte films deposited with various $N_2$/Ar flow ratios showed an increase of crystallization temperature and especially, the electrolyte films deposited at $N_2$/Ar ratios above 30% showed a crystallization temperature above $400^{\circ}C$, resulting in surviving in a back-end process in semiconductor memory devices. The device with a 200 nm thick $Cu_{1-x}(Ge_{45}Te_{55})_x$ electrolyte switches at 1 V from an "off " state resistance, $R_{off}$, close to $10^5$ to an "on" resistance state, Ron, more than 20rders of magnitude lower for this programming current.

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Characterization of Bacteriocin Production by Lactococcus lactis LAB3113 Isolated from Kimchi

  • Shin, Jong-Yeun;Cheol Ahn
    • Preventive Nutrition and Food Science
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    • 제2권2호
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    • pp.101-108
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    • 1997
  • A lactic acid bacterium LAB3113, isolated from traditionally fermented Kimchi was found to produce bacteriocin whose activity was very specific toward lactobacilli and not effective against any other bacteria. Lactobacilli affected by the inhibitory substance included Lactobacillus delbrueckii-lactis, L. johnsonii, L. gsseri, and L. curvatus. Based upon biochemical and physiological characteristics, LAB3113 was classified as Lactococcus lactis, and its bacteriocin was named as lactococcin K3113. Lactococcus lactis. LAB3113 produced bacteriocin at th early stage of growth and the concentration of the bacteriocin did not decrease even after alt stationalry phase. Optimal temperature of bacteriocin production was $25^{\circ}C$ at the initial pH 7.0. Partially purified lactococcin K3113 was completely inactivated by protease, but not affected by lipase, lysozyme and RNase. The bacteriocin was very heat-stable even after autoclaving for 20 min. It was also stable in pH changes, an was not affected by th presence of solvents. lacotococcin K3113 appeared to act in bactericidal mode against L. delbrueckii-lactis ATCC4797. Molecular weight of lactococcin K3113 was calibrated as 10,500 dal by SDS-PAGE an activity staining. Lactococcus lactis LAB3113 had four residential plasmids of 3.7kb, 11.2kb, 15.5kb, and 48kh in molecular sizes. Plasmid profile analysis of mutant strain revealed that 15.5 kb plasmid was re-sponsible for the production of lactococcin K3113 and its immunity to the bacteriocin.

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