• Title/Summary/Keyword: rbcS

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Phylogeny of the family Ophioglossaceae with special emphasis on genus Mankyua (제주고사리삼을 중심으로한 고사리삼과 식물의 계통)

  • Sun, Byung-Yun;Baek, Tae Gyu;Kim, Young-Dong;Kim, Chan Soo
    • Korean Journal of Plant Taxonomy
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    • v.39 no.3
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    • pp.135-142
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    • 2009
  • Phylogeny of the family Ophioglossaceae and a phylogenetic position of Mankyua were estimated through analyses of chloroplast rbcL gene sequences and spore morphology. Sequence analysis of the rbcL gene clearly indicated that there are two major lineages in the family Ophioglossaceae: Botrychioid lineage and Ophioglossoid lineage. The Botrichioid lineage is composed of three distinct clades: Botrychium, Helminthostachys and Mankyua, where Helminthostachys and Mankyua were placed as sister groups to the Botrychium. Within the genus Botrychium, subgenera Septridium and Botrychium were monophyletic, while taxa of subgen. Botrypus branched as sister of the two, successively, thus making a non-monophyletic group. Ophioglossum formed the Ophioglossoied lineage, where the subgen. Ophioglossum is monophyletic, while subgen. Cheiroglossa and Ophoderma formed a sister relationship with subgen. Ophioglossum. In terms of external morphology and spores, Mankyua is most similar to Helminthostachys, however, patristic distance in the cladogram and trophophore characteristics of the two genera are distinct. Therefore, Mankyua is a well defined genus within the family in terms of morphology as well as molecular phylogeny which places it in basal position of the Botrychioid lineage on the gene tree.

Distribution of Magnetic Field Depending on the Current in the μ-turn Coil to Capture Red Blood Cells (적혈구 포획용 미크론 크기 코일에 흐르는 전류의 크기에 따른 자기장 분포 특성)

  • Lee, Won-Hyung;Chung, Hyun-Jun;Kim, Nu-Ri;Park, Ji-Soo;Lee, Sang-Suk;Rhee, Jang-Roh
    • Journal of the Korean Magnetics Society
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    • v.25 no.5
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    • pp.162-168
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    • 2015
  • The ${\mu}$-turn coil having a width of ${\mu}m$ on the GMR-SV (giant magnetoresistance-spin valve) device based on the antiferromagnetic IrMn layer was fabricated by using the optical lithography process. In the case of GMR-SV film and GMR-SV device, the magnetoresistance ratios and the magnetic sensitivities are 4.4%, 2.0%/Oe and 1.6 %, 0.1%/Oe, respectively. In the y-z plane the distribution of magnetic field of GMR-SV device and $10{\mu}$-turns coil which put under the several magnetic bead(MB)s with a diameter of $1{\mu}m$ attached to RBC (red blood cell) was analyzed by the computer simulation using the finite element method. When the AC currents of 20 kHz from 0.1 mA to 10.0 mA flow to the 10 turns ${\mu}$-coil, the magnetic field at the position of $z=0{\mu}m$ at the center of coil was calculated from $30.1{\mu}T$ to $3060{\mu}T$ in proportion to the current. The magnetic field at the position of $z=10{\mu}m$ was decreased to one-sixth of that of $z=0{\mu}m$. It was confirmed that the $10{\mu}$-turn coil having enough magnitude of magnetic field for the capture of RBC is possible to use as a biosensor for the detection of magnetic beads attached to RBC.

Analysis of hematological changes in normal and diarrhea calves

  • Song, Ru-hui;Kang, Jin-hee;Park, Kwang-man;Youm, Jung-ho;Park, Jin-ho
    • Korean Journal of Veterinary Service
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    • v.43 no.3
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    • pp.161-165
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    • 2020
  • In order to effective treats a sick calf, it is necessary to obtain accurate information about the patient's condition. However, the standard references for Korean cattle claves are not well known. Therefore, this study aims to present useful clinical values by analyzing normal blood and diarrhea blood of Hanwoo calf. Recently, with the advent of a portable blood analyzer, it is possible to immediately analyze the patient's condition and severity in the field, not in the laboratory, and to calculate a suitable dosage for supporting fluid therapy. Therefore, in this study, the distribution of red blood cells (RBC) and white blood cells (WBC) were analyzed in normal and diarrhea Hanwoo calves. As a result, Hematocrit (HCT) levels increase significantly between 1 and 20 days in diarrhea positive calves. Changes in leukocyte composition had similar growth patterns in normal and diarrhea calves. As it grew, the proportion of neutrophils decreased and lymphocytes increased. However, the number of WBCs increased from 1 to 10 and 21 to 30 days in diarrhea positive calves, which is closely related to the increase in neutrophils. Therefore, those data can be used for diagnosis and treatment of diarrhea calf.

Cryptonemia rotunda (Halymeniales) and Schizymenia apoda (Nemastomatales), two new records of red algae from Korea

  • Kim, Su-Yeon;Seo, Tae-Ho;Park, Jeong-Kwang;Boo, Ga-Hun;Kim, Kyeong-Mi;Boo, Sung-Min
    • ALGAE
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    • v.27 no.1
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    • pp.1-8
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    • 2012
  • Here we report the first finding of Cryptonemia rotunda and Schizymenia apoda in Korea based on plastid rbcL sequence analysis and morphological observations. C. rotunda occurred in the subtidal in Hongdo and Namyeodo on the south coast. Thalli have short stipe with foliose, membranous blades with dentate margin, a length of 2-8 cm. S. apoda occurred in many locations along the coast of Korea. Thalli are foliose, simple or broadly lanceolate blades with irregular lobes and proliferations from the margins, sometimes undulate, soft fleshy, and slippery, up to 32 cm tall, and have cystocarps with ostioles on the blade. Plastid rbcL sequences reveal that each of these species is clearly separated from other species of their respective genera. S. apoda was well resolved within the genus. However, C. rotunda was distantly related to other members of the genus, and this result needs an urgent revision of the genus.

Influences on the Anticancer and Inhibitive Effects of the Secondary Effects by Anticarcinogen of Shibyukmiyouki-Eum (十六味流氣飮의 抗癌 및 抗癌劑 副作用에 미치는 영향)

  • Shim, Sang-Hee;Kim, Jong-Han;Choi, Jung-Hwa;Jeong, Hyun-Woo
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.15 no.2
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    • pp.302-314
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    • 2002
  • Shibyukmiyouki-Eum was a drug that treated carbuncle and cellulitis. So, the purpose of this study was to investigate effect of Shibyukmiyouki-Eum on the anticancer and inhibitive effects of the secondary effects by anticarcinogen We used Shibyukmiyouki-Eum extract(SYE) with freeze-dried, 8wks-old male mice(balb/c, ICR) and cancer cell lines(L1210. S-180) for this Study, The proliferation of cells was tested using a colorimetric tetrazoliun assay(MTT assay). and measurement of WBC, RBC, hemoglobin and platelet was tested by a automated hematology analyzer. The results of this study were obtained as follow; SYE was showed significantly cytotoxicity on the L1210 and S-180 cell lines. increased proliferation of thymocytes decreased by anticarcinogen. In combined effects of SYE and vincristine(0.005㎎/kg), SYE was significantly inhibited proliferation of L1210 cells and significantly increased proliferation of thymocytes. Also SYE was significantly increased count of WBC. platelet and increased count of RBC, hemoglobin. These results suggest that SYE has not only anticancar action but inhibitive effects of on the secondary effects by anticarcinogen.

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Identification of three independent fern gametophytes and Hymenophyllum wrightii f. serratum from Korea based on molecular data

  • LEE, Chang Shook;LEE, Kanghyup;HWANG, Youngsim
    • Korean Journal of Plant Taxonomy
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    • v.50 no.4
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    • pp.403-412
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    • 2020
  • Colonies of three independent gametophytes (one that is filamentous and two that are ribbon-like) without sporophytes occur in Gyeonggi-do, Gangwon-do, Gyeongsang-do, and Jeju-do, Korea. They have a moss-like appearance at first sight, with tiny plantlets and gemmae, and grow in cool, shaded, relatively deep dint places of large rocks, such as the small caves in high mountains, close to valleys. The gametophytes were identified based on morphological and molecular data by chloroplast DNA (cpDNA) sequence data (rbcL, rps4 gene and rps4-trnS intergenic spacer). Here, rbcL, rps4 gene and rps4-trnS intergenic spacer data of one independent gametophyte distributed in Korea have the same morphology, DNA sequence and monophyletic group as Crepidomanes intricatum from the eastern United States. They also share the same cpDNA data with Crepidomanes schmidtianum recently reported from Korea. The other independent gametophyte should be Hymenophyllum wrightii based on cpDNA data. The last one was presumed to be Pleurosoriopsis makinoi based on molecular data. The taxonomic status was confirmed to be the forma of Hymenophyllum wrightii through a revision of Hymenophyllum wrightii f. serratum based on molecular data.

Indirect calculation for volume of packed red cell(VPRC) by means of erythrocyte sedimentation rate(ESR) of diluted blood in dogs (개 회석혈의 적혈구침강율(ESR)에 의한 적혈구 침층용적(VPRC)치의 간접계산법)

  • Lee, Bang-whan;Park, Young-jun
    • Korean Journal of Veterinary Research
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    • v.29 no.2
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    • pp.41-49
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    • 1989
  • The study was undertaken to obtain indirect values of volume of packed red cell (VPRC) without centrifugation, using the erythrocyte sedimentation rate (ESR) of diluted blood in dogs. ESRs of diluted blood using the diluent of autologous plasma in which formed numerous RBC-rouleau clumps, autologous serum in which formed a few RBC-rouleau clumps, and 5% dextrose or 6% sucrose solutions in which formed numerous RBC-aggregation clumps, were accelerated. But, ESR of diluted blood using the 0.9% saline, D-S, ACD-B, CDP or D-PBS solutions were sluggish, because erythrocytes were dispersed in these diluents. Reliable values of VPRC on the basis of the correlating regressive equation to the ESR could be derived from values of$60^{\circ}$-angled-micro-ESR/40 min in the mixture, four parts of 5% dextrose solution and one part of whole blood. In the ESR values of diluted blood with low ratio, 1:1~3:1, $60^{\circ}$-micro-ESR was higher than $60^{\circ}$-Wintrobe-ESR. But, in the diluted blood with high ratio, 4:1~5:1, there was no different ESR values. For an aid of practical use, authers suggested a list of the $60^{\circ}$-micro-ESR/40 min in the diluted blood with equivalent VPRC of whole blood.

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Effect of Mahwang (Ephedrae herba) extract on serum Biological Values and Blood cell Composition (마황추출액이 혈액의 생물학적 수치 및 혈구 구성에 미치는 영향)

  • Park Young-Hoon
    • Korean Journal of Plant Resources
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    • v.19 no.2
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    • pp.296-299
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    • 2006
  • Effects of mahwang(Ephedrae herba) extract on plasma lipid composition, GOT, GPT activity and blood cell composition were investigated in rats. Concentrations of FFA was not significantly different in all treatment groups. Concentrations of triglyceride and total cholesterol in plasma were decreased in mahwang(Ephedrae herba) extract groups. However concentrations of HDL-cholesterol and LDL-cholesterol showed no significant difference in all treatment groups. GOT activities were increased in mahwang(Ephedrae herba) extract 300mg/kg group. However the values of GPT showed no significant difference in all treatment groups. Counts of RBC were increased in mahwang(Ephedrae herba) extract 300mg/kg group. Counts of WBC showed no significant difference in all treatment groups. In WBC composition, neutrophils were increased, but other cells were not affected by mahwang extract treatment.