• ALGAE
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• v.28 no.2
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• pp.149-160
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• 2013

Six species of the genus Symphyocladia are currently recognized worldwide, all of which are reported to grow on the Korean coast. We described a new species Symphyocladia glabra sp. nov. based on morphological and molecular evidence. The new species is characterized by mostly an erect and broad thallus, the parallel arrangement of numerous apical cells, completely fused congenital cells, a corticated basal portion of faint midrib with six to eight pericentral cells, rhizoids cutting off from pericentral cells, and absent vegetative trichoblasts. The new species has morphological similarity to S. marchantioides and S. jejuinsula. However, S. glabra sp. nov. is distinguished from S. marchantioides by the corticated lower portion of the thallus and the absence of vegetative trichoblasts, and S. jejuinsula by number of pericentral cells and the fact that the thallus does not taper upward. The phylogeny of rbcL sequences indicated that S. glabra sp. nov. is definitely a separate entity within the genus Symphyocladia.

• Korean Journal of Veterinary Research
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• v.46 no.4
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• pp.387-393
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• 2006

Progressing to the iron deficiency anemia was experimentally induced in 4 clinically healthy dogs by repeated phlebotomy to characterize hematologic features, serum iron values, and RBC indices. Abnormal RBC morphologies were also evaluated semiquantitatively on Wright's-stained blood films. Hematologic abnormalities in early stage of anemia included decreased both hematocrit and hemoglobin, and reticulocytosis, with no changes in mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) were represented. In intermediate stage, decreased serum iron concentration with microcytosis and hypochromia were prominent. In late stage, red cell distribution width and Mentzer's index were out of reference ranges in the majority of dogs. In this study microcytic anemia was appeared at the hemoglobin range of 5.1-7.2 g/dl. On most sampling days, platelet counts and white blood cells were within the reference ranges, with some minor variations. Iron deficiency was not necessarily associated with microcytic anemia. Judging from the sequential changes of both MCV and MCHC, 3 patterns of anemia were sequentially observed: initially normocytic normochromic, intermediate normocytic hypochromic or normocytic normochormic, and finally microcytic hypochromic. The most frequent morphologic abnormalities were target cells. Occasional elliptocyte, acanthocyte, stomatocyte, kinzocyte, dacrocyte and schistocyte were also noted on the blood films.

• Journal of Photoscience
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• v.9 no.2
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• pp.332-334
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• 2002

We examined the effects of supplementary ultraviolet-B (UV-B) radiation on the changes in synthesis and degradation of ribulose-I, 5-biphosphate carboxylase /oxygenase (Rubisco) and light-harvesting chlorophyll a/b binding protein of PSII (LHCII), as well as mRNA levels for small and large subunits of Rubisco (rbcS and rbcL, respectively) and LHCII (cab) with leaf age in UV-sensitive rice (Norin I) and UV-resistant rice (Sasanishiki). Both Rubisco and LHCII were actively synthesized until the leaf had fully expanded, and then decreased with leaf age. Synthesis of Rubisco, but not LHCII, was significantly suppressed by UV-B in Norin 1. The degradation of Rubisco was enhanced by UV-B around the time of the leaf maturation in the two cultivars. The levels of rbcS and rbcL were reduced by UV-B at the early leaf stages after emergence in both cultivars. The level of cab was first present at the highest level in the two cultivars, but drastically decreased due to UV-B treatment immediately after leaf emergence in Norin 1. It was proved that synthesis and degradation of Rubisco and LHCII greatly changed with leaf age: Rubisco synthesis was significantly suppressed by supplementary UV-B radiation at the transcription step during the early leaf stages. It was also suggested that the difference between the two rice cultivars in sensitivity to UV-B in the synthesis of Rubisco might be due to the specific suppression not only after transcription but also at transcription.

• Korean Journal of Plant Taxonomy
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• v.37 no.1
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• pp.1-15
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• 2007

Phylogenetic studies were conducted for 42 populations of Korean viola based on matK gene and atpB-rbcL intergenic spacer region of chloroplast DNA. In the matK tree, section Chamaemelanium and Dischidium were formed as a distinct group. Five subsections of section Nomimium were paraphyletic. In atpB-rbcL intergenic spacer region analysis, two species of sect. Chamaemelanium were monophyletic, and section Dischidium was placed sister to subsection patellares clade except for V. keiskei. Five subsections of section Nomimium were also paraphyletic as matK tree. the separate data analyses were incongruent in the relationships among 42 populations, especially for the position of section Dischidium and V. keiskei. The combined analyses of two chloroplast regions showed three major clades; section Chamaemelanium and Dischidium (x=6) formed a sister to subsections Hypocarpae and Trigonocarpae (x=10) clade; subsections Bilobatae and vaginatae (x=10 or 12) formed a clade with V. keiskei; and 19 populations of subsection patellares (x=12) except for V. keiskei were recognized as an independent clade within section Nomimium. Although combined data suggest three major clades of Korean viola, the origins of each clade from outgroup were discordance with previous ITS and trnL-F data.

• 순환기질환의공학회:학술대회논문집
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• 2002.11a
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• pp.60-61
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• 2002

• Proceedings of the Zoological Society Korea Conference
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• 1998.10b
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• pp.131.1-131
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• 1998

• Korea-Australia Rheology Journal
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• v.16 no.3
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• pp.129-134
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• 2004

The aggregation characteristics of red blood cells (RBCs) were measured using a newly developed light-transmission slit rheometer. Conventional methods of RBC disaggregation such as the rotational Couette system were replaced with a pressure-driven slit flow system with a vibrational mechanism. Using a vibration generator, one can disaggregate the RBC aggregates stored in the slit. While shear stress decreases exponentially, instantaneous pressure and the transmitted light intensity were measured over time. Applying an abrupt shearing flow after disaggregation caused a rapid elongation of the RBCs followed by loss of elongation with the decreasing shear stress. While the shear stress is further decreasing, the RBCs start to re-aggregate and the corresponding transmitted intensity increases with time, from which the aggregation indices can be obtained using a curve-fitting program.

• Clinical and Experimental Pediatrics
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• v.59 no.2
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• pp.100-103
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• 2016

Patients with hemolytic-uremic syndrome (HUS) can rapidly develop profound anemia as the disease progresses, as a consequence of red blood cell (RBC) hemolysis and inadequate erythropoietin synthesis. Therefore, RBC transfusion should be considered in HUS patients with severe anemia to avoid cardiac or pulmonary complications. Most patients who are Jehovah's Witnesses refuse blood transfusion, even in the face of life-threatening medical conditions due to their religious convictions. These patients require management alternatives to blood transfusions. Erythropoietin is a glycopeptide that enhances endogenous erythropoiesis in the bone marrow. With the availability of recombinant human erythropoietin (rHuEPO), several authors have reported its successful use in patients refusing blood transfusion. However, the optimal dose and duration of treatment with rHuEPO are not established. We report a case of a 2-year-old boy with diarrhea-associated HUS whose family members are Jehovah's Witnesses. He had severe anemia with acute kidney injury. His lowest hemoglobin level was 3.6 g/dL, but his parents refused treatment with packed RBC transfusion due to their religious beliefs. Therefore, we treated him with high-dose rHuEPO (300 IU/kg/day) as well as folic acid, vitamin B12, and intravenous iron. The hemoglobin level increased steadily to 7.4 g/dL after 10 days of treatment and his renal function improved without any complications. To our knowledge, this is the first case of successful rHuEPO treatment in a Jehovah's Witness child with severe anemia due to HUS.

• Journal of Species Research
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• v.9 no.3
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• pp.221-232
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• 2020

Selaginella vaginata is a common montane species with broad distribution in China and the Himalaya region, and several species that are morphologically similar to S. vaginata are distributed in Asia. The taxonomic revision of S. vaginata and related species was performed by morphological comparison of leaves, strobili, and spores, and phylogenetic analysis. Based on these results, a new species, S. subvaginata, sp. nov., has been identified. Morphologically, S. subvaginata has intermediate form between S. vaginata and S. repanda, which differs mainly in its main stem being erect, dorsal leaves long-ciliolate on inner margin and outer margin denticulate or with 2-4 cilia at base (long-ciliolate on both inner and outer margins in S. vaginata, denticulate on both inner and outer margins in S. repanda), and acroscopic base of ventral leaves long ciliolate (sparsely long ciliolate in S. vaginata, short ciliolate to denticulate in S. repanda). Moreover, phylogenetic analysis using three chloroplast markers(rbcL, atpI, and psbA) revealed that S. subvaginata is a distinct species among the anisosporophyllous species clade in Selaginellaceae.

• The Korean Journal of Nuclear Medicine Technology
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• v.12 no.1
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• pp.39-43
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• 2008

Purpose: There are various methods to diagnose hemangioma, such as ultrasonography (US), computed tomography (CT), magnetic resonance imaging (MRI) and nuclear medicine. However, by development of SPECT imaging, the blood-pool scan using $^{99m}Tc$-labeled red blood cell has been used, because it was non-invasive and the most economical method. Therefore, in this study, we proposed that the usefulness of $^{99m}Tc$-RBC scan and SPECT of the head and neck to diagnose unlocated hemangiomas. Materials and Methods: $^{99m}Tc$-RBC scan and SPECT was performed on 6 patients with doubtful hemangioma (4 person, head; 1 person, neck; 1 person, another). We labeled radiopharmaceutical using modified in vivo method and then, centrifuged it to remove plasma. After a bolus injection of tracer, dynamic perfusion flow images were acquired. Then, anterior, posterior, both lateral static blood-pool images were obtained as early and 4 hours delayed. SPECT was progressed 64 projections per 30 seconds. Each image was interpreted by physicians, Nuclear medicine specialist, and technologist blinded to patient's data. Results: In 5 patients of all the radioactivity of doubtful site didn't change in flow images, but, in blood-pool, delayed and SPECT images, it was increased. So, it was a typical hemangioma finding. The size of lesion was over 2 cm, and it could discriminate as comparing to the delayed and SPECT imaging. On the other hand, in 1 patient, the radioactivity was increased in blood-pool images, but, not in delayed and SPECT images, so, it was proved no hemangioma. Conclusion: Using $^{99m}Tc$-RBC Scan and SPECT, we could diagnose the hemangiomas in head and neck, as well as, liver, more non-invasive, economical, and easy. Therefore, it considered that $^{99m}Tc$-RBC scan and SPECT would offer more useful information for diagnosis of hemangioma, rather than otherimaging such as US, CT, MRI.