• 한국미생물·생명공학회지
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• 제21권5호
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• pp.461-467
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• 1993

Transglycosylation reaction of cyclodextrin glucanotransferase (CGTase) was analyzed in the attrition coupled heterogeneous reaction system using raw starch as a donor` and mono-, di-saccharide, and glycoside as acceptors. For transglycosylation reaction of stevioside, the transglycosylation rate was similar and the transglycosylation yield was increased compare with conventional process using liquefied starch as the donor. Also the accumulation of maltooligosaccharides in reaction mixture was minimized.

• 한국미생물·생명공학회지
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• 제22권3호
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• pp.252-258
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• 1994

Transglycosylation of stevioside in the attrition coupled heterogeneous reaction system using raw starch as a glycosyl donor has significant advantages over conventional reaction systems using liquefied starch as a donor. The transglycosylation of stevioside under the presence of organic solvent showed that transglycosylation reaction occurs via two steps ; initially from raw starch to cyclodextrin(CD), and then followed by transglycosylation of produced CD. Comparison of the transglycosylation efficiency of c$\alpha $-, $\beta $, $\gamma $-CDs indicated that $\alpha $-, $\beta $-CD are mainly utilized as a glycosyl donor for following reaction. The reaction mechanism of transglycosylation between stevioside and CD proceeded according to random sequential bireactant mechanism. The equilibrium constant of transglycosylation reaction of cyclodextrin glucanotransferase wase also evaluated. The structure of transglycosylated stevioside was confirmed by TLC, and it was found that glycosyl group(G$_{1}, $ ~ G$_{4}$-glycosidic bond.

• KSBB Journal
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• 제9권2호
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• pp.108-114
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• 1994

분쇄마찰매치 함유 불균일상 효소반응계에서 생전분을 당공여체로 한 stevioside의 당전이 반응의 kinetics에 관한 연구를 수행하였다. 생전분으로부터 CD를 생서하는 과정과 생성된 CD와 stevioside가 random sequenrial bireactant ,echanism으로 반응하여 당전이 equation을 유도하였다. 또한 유도된 반응식의 각종 kinetic constants을 평가하였다. 유도된 반응식을 Runge-Kutta integration법으로 계산하였으며, 계산 결과를 실험치와 비교하여 유도식의 효용성을 검토하였다. 유도된 kinetic equations는 당공여체인 생전분의 농도, stevioside의 농도, 그리고 중간산물인 CD의 농도 변화를 비교적 정획히 표시할 수 있었으며, 고효율 당전이 효소반응기 개발에 활용될 것이다.