• 한국약용작물학회지
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• 제28권6호
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• pp.445-454
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• 2020

Background: Culturing wild ginseng adventitious root using plant factory technology provides genetic safety and high productivity. This production technology is drawing attention in the fields of functional raw materials and product development. The cultivation method using elicitors is key technology for controlling biomass and increasing secondary metabolites. Methods and Results: Elicitor treatments using methyl jasmonate, pyruvic acid, squalene, β-sistosterol were performed to amplify total ginsenosides (Rb1, Rc, Rb2, Rb3, and Rd) of cultured wild ginseng adventitious root. Thereafter, fermentation and steaming processes were performed to convert total ginsenosides into minor molecular ginsenosides (Rg3, Rk1, and Rg5). The result indicated that methyl jasmonate minimizes the reduction in fresh weight of cultured wild ginseng adventitious root and maximizes total ginsenosides (sum of Rb1, Rc, Rb2, Rb3, and Rd). Ginsenoside conversion results showed a maximum degree of conversion of 131 mg/g. Conclusions: In this study, we demonstrated that the optimal elicitor treatment method increased the content of total ginsenosides, while the steaming and fermentation processing method increased the content of minor ginsenosides.

• 한국식품영양학회지
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• 제27권2호
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• pp.240-248
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• 2014

본 연구는 돈육 중의 후지육을 이용하여 천연 향신료인 생강 및 인삼 분말을 첨가비율별로 처리(무처리 대조구, T1구(A-생강 분말 0.1% 첨가, B-인삼 분말 0.1% 첨가), T2구(A-생강 분말 0.2% 첨가, B-인삼 분말 0.2% 첨가), T3구(A-생강 분말 0.3% 첨가, B-인삼 분말 0.3% 첨가)하여 돈육포를 제조한 후, 저장기간에 따른 VBN, 미생물 수 및 관능평가에 미치는 영향을 조사하였다. 돈육포의 VBN 함량은 대조구와 처리구간에서는 통계적인 유의차가 나타내었고(p<0.01), 저장기간이 길어짐에 따라 VBN 함량과 총 세균수 및 대장균 수도 점차 증가하는 경향을 나타내었다. 또한 처리구에 상관없이 생강 분말 첨가군(A)이 인삼 분말 첨가군(B)군보다 유의적으로 낮게 나타났다(p<0.01). 돈육포의 관능평가의 경우, 육색과 맛은 처리구에 상관없이 저장기간이 길어짐에 따라 저장 2주째에 가장 높아 통계적인 유의차가 나타났고(p<0.05), 그 이후는 서서히 낮아졌다. 특히 육색의 경우, T1구의 A군이 다른 처리구보다 크게 높게 나타났다(p<0.05). 이상의 결과로 볼 때 돈육포 제조 시 생강 분말 첨가는 미생물의 증식 억제에 다소 영향을 미치며, 육포 제조 후 2주째에 가장 맛이 좋은 것으로 판단된다.

• 한국식품영양과학회지
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• 제37권3호
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• pp.261-268
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• 2008

청국장을 다양한 식품소재로 활용하기 위하여 각 조건별 청국장(CGJ), 홍삼청국장(RCJ) 및 홍삼청국장 가수분해물(RCH)의 품질 및 기능적 특성을 조사하였다. 일반성분은 RCJ가 탄수화물 함량이 다른 시료에 비하여 높았으며 조단백 함량은 낮았다. 유리아미노산 함량은 CGJ에 비하여 RCH가 2,157.16 mg%로 약 2배정도 높았으며 필수아미노산 함량도 812.18 mg%로 가장 높았다. SDS-전기영동 패턴분석 결과 CGJ와 RCJ는 33 kDa 이하의 분자량을 나타내었고, RCH는 17 kDa 이하의 저분자 분자량으로 나타나 저분자화 가수분해되었음을 확인할 수 있었다. DPPH free radical 소거활성, superoxide radical 소거활성, ACE 저해활성은 시료 간에 유의적 차이는 크게 나타나지 않았으나, RCH가 가장 높은 활성을 보였고, 혈전용해 활성은 CGJ가 111.38 unit로 가장 높게 나타났다. 또한 관능평가에서 홍삼 특유의 쓴맛은 있었으나 전반적 기호도는 높게 나타났다. 이상의 결과 청국장(CGJ)에 비하여 홍삼청국장(RCJ) 및 홍삼청국장 가수분해물(RCH)이 기능성 강화 및 불쾌취가 감소되어 다양한 식품 소재로 활용이 가능할 것으로 기대된다.

• 한국식품영양과학회지
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• 제37권1호
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• pp.72-75
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• 2008

경북 풍기지역의 연근별 수삼을 이용한 홍삼제조에 따른 전반적인 품질특성을 분석하였다. 그 결과 5년근 홍삼은 색택, 외관, 직경, 내부품질 등에서 6년 근과 유사하거나 다소 저조하였으나, 길이와 수율 면에서는 우수한 것으로 나타났다. 또한 홍삼의 유효성분인 ginsenoside 함량은 5년 근이 홍삼의 특이성분이라고 할 수 있는 ginsenoside $Rg_3$ 및 $Rh_2$ 함량이 더 높은 것으로 나타났다. 따라서 경북 풍기지역의 홍삼제조에 있어서는 5년 근 수삼을 원료삼으로 사용하는 것이 전반적인 품질 및 유효성분 측면에서 매우 타당한 것으로 평가되었다.

• 한국식품영양과학회지
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• 제38권5호
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• pp.587-593
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• 2009

당액에 수삼을 넣고 졸이는 횟수에 따라 얻어진 인삼정과 및 정과액의 품질특성을 조사하여 인삼정과의 품질향상을 위한 기초자료를 얻고자 하였다. 인삼정과는 수삼 8 kg을 2배량의 물 16 kg을 넣어 5분간 삶은 후 삶은 물 8 kg을 제거하고 설탕 10 kg을 넣어서 60분간 졸여서 24시간 당침하는 작업을 5회에 걸쳐 행하였다. 졸임 횟수가 증가함에 따라 정과와 정과액 모두 수분함량과 pH는 낮아졌고 정과액의 $^{\circ}Brix$는 상대적으로 높아졌다. 정과 내 조사포닌 함량은 원료삼의 4배가 증가하였으며 구성사포닌 중 Rf와 Rd의 함량이 많이 증가하여 각각 원료삼의 77배 및 16배가 높아졌으며 기타 검량된 구성사포닌은 감소하였다. 정과액에서도 정과에서와 같은 경향으로 인삼의 사포닌이 용출되어 마지막 정과액(GJS 5)에서 Rf와 Rd 및 조사포닌 함량이 각각 15.05, 3.54 및 61.88 mg/g이었다. 또한 졸임 과정 동안 수분증발에 의하여 정과 및 정과액 모두 총 당 함량은 증가하였으며 졸이는 동안 glucose와 fructose의 함량도 증가하였다. 정과의 유기산 함량은 당을 첨가하여 졸이기 시작하면서 급격하게 감소하였으며, 정과액은 졸임 횟수가 증가할수록 malic acid와 citric acid가 증가하는 경향이었다. 정과 및 정과액의 색도는 졸임 횟수가 증가할수록 L값은 낮아지고 a값과 b값은 증가하는 경향이었다.

• Natural Product Sciences
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• 제10권3호
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• pp.134-139
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• 2004

In this study, an immunoactivity of panaxtriol ginsenosides Re and Rg1 against infection due to Candida albicans was investigated. The ginsenosides were extracted from Red Ginseng with 85% ethanol and heat-treatment and were analyzed by HPLC on water-acetonitrile as a mobile phase. The HPLC analysis revealed that the extract contained ginsenosides Re and Rg1, which were eluted as a combined peak. By agar diffusion susceptibility, the mixture of Re and Rg1 had no growth-inhibitory activity on C. albicans yeast cells. However, in animal tests BALB/c mice given the mixture of Re and Rg1 intraperitoneally (Lp.) before intravenous (Lv.) infection with live C. albicans yeast cells had longer mean survival times (MST) than MST of control mice groups that received only buffer solution instead of Re and Rg1. In experiments 60% of the ginsenosides-treated mice survived the entire duration of the 50-day observation. The Re and Rg1 mixture induced production of nitric oxide when interacted with RAW 264.7 macrophage cell line. In addition, the mixture caused morphological change of the macrophages. These data indicate that immunostimulation by the Re and Rg1 may be responsible for the protection of mice against disseminated candidiasis.

• Preventive Nutrition and Food Science
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• 제19권1호
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• pp.10-18
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• 2014

In this study, Woongjin fermented red ginseng extract (WFRG) was evaluated for its potential ability to act as an adjuvant for the immune response of mice. For the in vitro study, macrophages were treated with serial concentrations ($1{\mu}g/mL$, $10{\mu}g/mL$, and $100{\mu}g/mL$) of WFRG. For in vivo studies, mice were administered different concentrations (10 mg/kg/day, 100 mg/kg/day, and 200 mg/kg/day) of WFRG orally for 21 days. In vitro, the production of nitric oxide and TNF-${\alpha}$ by RAW 264.7 cells increased in a dose-dependent manner. In vivo, WFRG enhanced the proliferation of splenocytes induced by two mitogens (i.e., concanavalin A and lipopolysaccharide [LPS]) and increased LPS-induced production of TNF-${\alpha}$ and IL-6, but not IL-$1{\beta}$. In conclusion, WFRG has the potential to modulate immune function and should be further investigated as an immunostimulatory agent.

• Journal of Ginseng Research
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• 제38권3호
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• pp.208-214
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• 2014

Background: In previous work, we reported that Korean Red Ginseng saponin fraction (RGSF) showed anti-inflammatory activities in vitro and in vivo. Methods: The present study investigated the radioprotective properties of RGSF by examining its effects on ionizing radiation (IR)-enhanced and lipopolysaccharide (LPS)-mediated inflammatory responses in murine macrophage cells. Results: RGSF induced strong downregulation of IR-enhanced and LPS-induced proinflammatory responses such as nitric oxide (NO) production (Inhibitory Concentration $50(IC_{50})=5.1{\pm}0.8{\mu}M$) and interleukin-$1{\beta}$ levels. RGSF was found to exert its radioprotective effects by inhibition of a signaling cascade that activated checkpoint kinase 2enuclear factor-${\kappa}B$. In addition, RGSF strongly inhibited IR-enhanced LPS-induced expression of hemoxyganase-1, implying that the latter may be a potential target of RGSF. Conclusion: Taken together, our data suggest that RGSF can be considered and developed for use as an effective radioprotective agent with minimal adverse effects.

• Journal of Ginseng Research
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• 제35권2호
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• pp.200-208
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• 2011

Ginsenoside (G) $Rp_1$ is a ginseng saponin derivative with anti-cancer and anti-inflammatory activities. In this study, we examined the mechanism by which G-$Rp_1$ inhibits inflammatory responses of cells. We did this using a strategy in which DNA constructs containing cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) promoters were transfected into HEK293 cells. G-$Rp_1$ strongly inhibited the promoter activities of COX-2 and iNOS; it also inhibited lipopolysaccharide induced upregulation of COX-2 and iNOS mRNA levels in RAW264.7 cells. In HEK293 cells G-$Rp_1$ did not suppress TANK binding kinase 1-, Toll-interleukin-1 receptor-domain-containing adapter-inducing interferon-${\beta}$ (TRIF)-, TRIF-related adaptor molecule (TRAM)-, or activation of interferon regulatory factor (IRF)-3 and nuclear factor (NF)-${\kappa}$B by the myeloid differentiation primary response gene (MyD88)-induced. However, G-$Rp_1$ strongly suppressed NF-${\kappa}$B activation induced by I${\kappa}$B kinase (IKK)${\beta}$ in HEK293 cells. Consistent with these results, G-$Rp_1$ substantially inhibited IKK${\beta}$-induced phosphorylation of $I{\kappa}B{\alpha}$ and p65. These results suggest that G-$Rp_1$ is a novel anti-inflammatory ginsenoside analog that can be used to treat IKK${\beta}$/NF-${\kappa}$B-mediated inflammatory diseases.

• 한국해양바이오학회지
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• 제14권2호
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• pp.61-68
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• 2022

This study aimed to investigate the hepatoprotective activities of the sea cucumber products, including extracts and hydrolysates, in vitro and in vivo. Dried sea cucumber, produced on the western coast of Korea, was boiled in water or 70% ethanol at 85℃ or 100℃ for 18 or 24 h, respectively, to extract bioactive compounds. The enzymatic hydrolysates were prepared by reacting the dried sea cucumber with pepsin or neutral protease (PNL) under optimal enzyme conditions. The anti-inflammatory effect of the samples was investigated using RAW 264.7 cells treated with lipopolysaccharide (LPS). The amount of nitric oxide (NO) was produced from the cells treated with LPS and each sample was compared. Therefore, the pepsin hydrolysate treatment decreased NO production compared to LPS sole treatment. Furthermore, the effects of the samples on cell injury in the hepatic cell line and bisphenolA-induced hepatic injury mouse model were investigated. The water extracts and the pepsin hydrolysates of sea cucumber significantly inhibited cell injury generated in the hepatocytes without cytotoxicity (p < 0.05), whereas the ethanol extracts were cytotoxic. However, these results indicate that the extracts and the enzymatic hydrolysates derived from sea cucumber can be used as beneficial materials for inhibiting liver damage.