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Effects of Gamisoyosan(GS) on LDL Oxidation in RAW 264.7 Cell. (가미소요산(加味逍遙散)이 지단백산화(脂蛋白酸化)에 따른 RAW 264.7 활성(活性)에 미치는 영향(影響))

  • Hwang Gwi-Seo
    • Journal of Society of Preventive Korean Medicine
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    • v.5 no.1
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    • pp.134-143
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    • 2001
  • The oxidative modification of low density lipoprotein(LDL) has been implicated in the development of atherosclerosis. Oxidized LDL are found in macrophage foam cell, and it can induce an macrophage proliferation in atherosclerotic plaque. In this study, we investigated the hypothesis that gamisoyosan(GS) may reduce atherosclerosis by lowering the oxidiazability of LDL, To achive this goal, we examined the effect of GS on LDL oxidation, nitric oxide production in mouse macrophage cell line, RAW264.7, and the effect of GS on cupuric sulfate-induced cytotoxicity, LDH release, and macrophage activity. GS inhibited the generation of oxidized LDL from native LDL in RAW264.7 cell culture, and decreased the release of LDH from cupric sulfate-stimulated RAW264.7 cell. In other experiments, GS activated RAW264.7 cell, and prolonged the survival time, and increased nitric oxide production in Raw 264.7 cells.

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A Study on the Introduction of Raw Image File Formats for the Management of Digital Photographic Records (디지털 사진기록물 관리를 위한 Raw 이미지 파일 포맷의 도입에 관한 연구)

  • Park, Junyoung;Lee, Myounggyu
    • Journal of Korean Society of Archives and Records Management
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    • v.19 no.3
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    • pp.155-178
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    • 2019
  • The study aims to analyze the characteristics and problems of raw image file formats, evaluate its suitability for the management of digital photographic records, and suggest the introduction method to utilize such format in public institutions. Raw image file formats feature reversibility and flexibility. However, most of them are proprietary file formats that depend on the digital camera manufacturer. The DNG format, which was developed to standardize raw image file formats, has received positive reviews from overseas public agencies. However, the DNG format is not fully recognized as a standard; hence, it is appropriate to use it tentatively until the standard raw format appears. On the other hand, the converted digital photo file format is not recognized as an original with legal authenticity. Therefore, in the short term, DNG files created by format conversion and original raw files should be encapsulated and preserved. Furthermore, it is necessary to redefine the baseline of the hash value and file size to recognize the legal authenticity only with DNG files in the long term.

A Study on Estimation of Metabolizable Energy Content in Cereal (주요곡물의 에너지 함량 평가에 관한 연구)

  • 김은미
    • Journal of Nutrition and Health
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    • v.29 no.1
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    • pp.50-58
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    • 1996
  • This study was undertaken to determined the metabolizable energy of cereal by the different method. Raw or cooked cereal foods were freeze-dried and fed to Sprague Dawley rat with 200-300g body weight to measure apparent metabolizable energy(AME) values and nitrogen-corrected AME(AMEn) values for four days after three days of preliminary period for adaptation to the diets. The AME values of Karaeddok, raw rice, cooked rice, raw brown rice, raw glutinous rice, cooked glutinous rice, raw barley and cooked barley applerared 4516.1, 3380.6, 4072.2, 3457.0, 4448.0, 2929.4 and 3780.2kcal/kg dry matter, respectively. The AMEn values of karaeddok, raw rice, cooked rice, raw brown rice, cooked brown rice, raw glutinous rice, cooked glutinous rice, raw barley and cooked barley appeared 4421.5, 3349.6, 4160.0, 3918.7, 4039.3, 3572.0, 4552.5, 3009.9 and 3873.4kcal/kg dry matter, respectively. A slight difference was observed when the AME values of the cereals measured in present study were compared with the energy values calculated by various conversion parameters such as Atwater's, Rubner's, Sochun's adn FAO's, indicating that the latter energy values by all conversion factors are acceptabel for several cereals.

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Production of Cyclodextrin from Raw Starch in the Agitated Bead Reaction System and its Reaction Mechanism (분쇄마찰매체 함유 효소반응계에서의 Cyclodextrin 생성과 Cyclodextrin Glucanotransferase의 작용 Mechanism)

  • Han, Il-Keun;Lee, Yong-Hyun
    • Microbiology and Biotechnology Letters
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    • v.19 no.2
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    • pp.163-170
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    • 1991
  • Production of cyclodextrin (CD) directly from raw corn starch without liquefaction using cyclodextrin glucanotransferase (CGTase) was carried out in an agitated bead reaction system. Similar CD yield and production rate comparable with those of conventional method using liquefied starch were obtained. Especially high purity-CD in the reaction mixture without accumulation of malto-oligosaccharides was obtained. The maximum 54g/l of CD was obtained at raw starch concentration of 200g/l. CD yield was inversely proportional to raw starch concentration, and conversion yield was 0.48 at substrate concentration of 100g/l. The optimal amount of enzyme (CGTase unit/g raw starch) was found to be around 6.0. Granular structure of raw starch degraded by CGTase was observed by SEM in order to investigate the enhancing mechanism, along with those of acid or alkali pretreated raw starch, amylose, and amylopectin. Kinetic constants of CGTase on raw starch in an agitated bead reaction system were evaluated, and CGTase was competitively inhibited by CD.

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Quality Characteristics of Raw and Dried Astragalus membranaceus Extracts (건조유무에 따른 황기 추출물의 특성)

  • Min, Sung Hee
    • Korean journal of food and cookery science
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    • v.33 no.1
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    • pp.65-71
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    • 2017
  • Purpose: This study was carried out to investigate the availability of raw Astragalus membranaceus as a food ingredient. Methods: Raw and dried A. membranaceus water extracts were used to examine the free radical scavenging activity, polyphenol and flavonoid contents, and antimicrobial activity. Also, the sensory characteristics of each water extracts were evaluated. Results: The free radical scavenging activity and total polyphenol and flavonoid contents of raw A. membranaceus extracts were higher than those of dried A. membranaceus extracts. The antimicrobial activities of raw and dried A. membranaceus extracts were examined against several food borne illness-causing microorganisms using the paper disc diffusion method. Inhibition zones were observed in Staphylococcus aureus subsp. aureus, Bacillus subtilis, Pseudomonas aeruglnosa, Salmonella enterica subsp. enterica, Escherichia coli and Bacillus cereus treated with raw extracts. Inhibition zones were observed in B. subtilis, P. aeruglnosa and S. enterica subsp. enterica treated with dried extracts. Raw A. membranaceus extracts had higher scores than dried extracts in sensory evaluation. Conclusion: Raw A. membranaceus was better in antioxidative, antimicrobial activities, and sensory characteristics than dried one.

Combustion and Mechanical Properties of Fire Retardant Treated Waste Paper-Waste Acrylic Raw Fiber Composite Board

  • Eom, Young Geun;Yang, Han-Seung;Kim, Hyun-Joong
    • Journal of the Korean Wood Science and Technology
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    • v.31 no.3
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    • pp.1-10
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    • 2003
  • Shredded waste newspapers, waste acrylic raw fibers, and urea-formaldehyde (UF) adhesives, at 10% by weight on raw material, were used to produce recycled waste paper-waste acrylic raw fiber composite boards in laboratory scale experiments. The physical and mechanical properties of fire retardant treated recycled waste paper-waste acrylic raw fiber composite boards were examined to investigate the possibility of using the composites as internal finishing materials with specific gravities of 0.8 and 1.0, containing 5, 10, 20, and 30(wt.%) of waste acrylic raw fiber and 10, 15, 20, and 25(wt.%) of fire retardant (inorganic chemical, FR-7®) using the fabricating method used by commercial fiberboard manufacturers. The bending modulus of rupture increased as board density increased, decreased as waste acrylic raw fiber content increased, and also decreased as the fire retardant content increased. Mechanical properties were a little inferior to medium density fiberboard (MDF) or hardboard (HB), but significantly superior to gypsum board (GB) and insulation board (IB). The incombustibility of the fire retardant treated composite board increased on increasing the fire retardant content. The study shows that there is a possibility that composites made of recycled waste paper and waste acrylic raw fiber can be use as fire retardant internal finishing materials.

Changes of the Chemical and Microbiological Quality in Milk from Jeju-Do after Raw Milk Grading System (등급제 실시 이후 제주산 원유의 품질 변화)

  • Lee, Hyun-Jong
    • Journal of Dairy Science and Biotechnology
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    • v.24 no.1
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    • pp.1-7
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    • 2006
  • This experiment was performed to identify the improvement of raw milk quality after introducing raw milk grading system(1993, June). The purpose of this experiment was to investigate chemical component and microbiological quality of raw milk in jeju. This experiment made it possible to spread high standard of quality of raw milk or milk product including yoghurt, ice cream etc., and to provide dairy industry information for the construction of Jeju international free city master plan. As a result, automatic milking system is improved a lot after introducing raw milk grading system and sustained good condition compared with other provinces. High ratio was shown dairy farm in jeju for pre-milking, pre-cooling system equipment and self laboring. Otherwise, the ratio of dairy farm doing test of mastitis is low. The ratio of first grade distribution in Jeju is 80.64%, which means that was improved before introducing raw milk grading system. The number of somatic cells found in summer more than that of other seasons in raw milk. However, these data is a little higher than the nation wide data medium. Also, general components, annual lipid ratio is 3.90% that improved compared with before introducing raw milk grading system. These data showed low in summer and similar to nation wide.

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The Study on the Weight loss Finishing for the Mixture of Silk/Polyester I. The Weight loss Finishing for the Raw-silk/Polyester (Silk/Polyester 혼섬유 재료의 감량가공에 관한 연구 I. Raw-silk/Polyester 혼섬유의 감량가공)

  • 배도규
    • Journal of Sericultural and Entomological Science
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    • v.35 no.2
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    • pp.114-119
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    • 1993
  • The weight loss for the mixture(raw-silk/PET) was tested to investigate the effects of weight loss accelerator on the degumming and weight loss. The degumming loss of raw-silk was decreased while the weight loss polyester(PET) was increased depending on the increase of accelerator concentration. The difference of the weight loss was not significant in raw-silk but the weight loss of PET was increased with the increase of the alkali concentration. The proper degumming of raw-silk was obtained and simultaneously the weight loss of PET was high for the raw-silk/PET "A" and "B" type. The proper degumming of raw-silk was obtained but the weight loss of PET was low for the raw-silk/PET "D" and "E" type. It showed that the typical degumming curve in raw-silk part and the weight loss of PET was increased depending on the treatment time in the weight loss for the fabric composed of raw-silk and PET.

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Analysis of Seasonal and Regional Changes in Major Food Components of Raw Laver Pyropia sp. (물김(Pyropia sp.)의 생산 시기별 및 지역별 주요 식품 성분 변화 분석)

  • Kang, Min-Gyun;Jeong, Min-Chul;Park, Seul-Ki;Lee, Jang-Won;Cho, Jeung-Hyung;Eom, Sung-Hwan;Huh, Man-Kyu;Kim, Young-Mog
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.51 no.5
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    • pp.510-517
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    • 2018
  • The study investigated the seasonal and regional changes in the food components of raw laver Pyropia sp.. Samples were obtained monthly from December 2017 to March 2018 at auction markets located in Gangseo-gu, Busan (Nakdong-raw laver), Seocheon-gun, Chungnam, Wando-gun, Jeonnam and Goheung-gun, Jeonnam. The general components, heavy metals and minerals, free amino acids, total phenolic compounds (TPC), and antioxidant activities of the samples were analyzed. No significant physicochemical changes in the moisture, crude lipid, and ash contents were observed in the raw lavers tested. However, the crude protein content of Nakdong-raw laver was the highest in December 2017 at $7.20{\pm}0.28%$ and the content was higher seasonally and regionally than those of the other raw lavers. In addition, the taurine, zinc, manganese and TPC contents of Nakdong-raw laver was higher seasonally and regionally than those of the other raw lavers. The ethanolic extract of Nakdong-raw laver also had higher antioxidant activity, including as DPPH (2,2'-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis [3-ethylbenzothiazoline-6-sulphonic acid]) radical scavenging activity, although there was no significant correlation between TPC and the antioxidant activity. This study is the first analysis of seasonal and regional changes in the major food components of raw laver cultivated in Korea.

Risk assessment of vibriosis by Vibrio cholerae and Vibrio vulnificus in whip-arm octopus consumption in South Korea

  • Oh, Hyemin;Yoon, Yohan;Ha, Jimyeong;Lee, Jeeyeon;Shin, Il-Shik;Kim, Young-Mog;Park, Kwon-Sam;Kim, Sejeong
    • Fisheries and Aquatic Sciences
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    • v.24 no.6
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    • pp.207-218
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    • 2021
  • This study evaluated the risk of foodborne illness from highly pathogenic Vibrio spp. (Vibrio vulnificus and V. cholerae) by raw whip-arm octopus (Octopus minor) consumption. In total 180 samples of raw whip-arm octopus were collected from markets and examined for the prevalence of V. vulnificus and V. cholerae. Predictive models describing the kinetic behavior of Vibrio spp. in raw whip-arm octopus were developed, and the data on amounts and frequency of raw whip-arm octopus consumption were collected. Using the collected data, a risk assessment simulation was conducted to estimate the probability of foodborne illness raw whip-arm octopus consumption using @RISK. Initial contamination levels of Vibrio spp. in raw whip-arm octopus were -3.9 Log colony-forming unit/g, as estimated by beta distribution fitting. The developed predictive models were appropriate to describe Vibrio spp. in raw whip-arm octopus during distribution and storage with R2 values of 0.946-0.964. The consumption frequency and daily consumption amounts of raw whip-arm octopus per person were 0.47% and 57.65 g, respectively. The probability of foodborne illness from raw whip-arm octopus consumption was estimated to be 8.71 × 10-15 for V. vulnificus and 7.08 × 10-13 for V. cholerae. These results suggest that the risk of Vibrio spp. infection from raw whip-arm octopus consumption is low in South Korea.