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Effect of embryo and recipient condition on pregnancy rate following bovine embryo transfer (소 수정란이식(受精卵移植)에 있어서 수란우(受卵牛)와 수정란(受精卵)의 조건이 수태율(受胎率)에 미치는 영향)

  • Lee, Jung-ho;Park, Hang-kyun;Shin, Sang-tae
    • Korean Journal of Veterinary Research
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    • v.29 no.1
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    • pp.61-68
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    • 1989
  • This study was carried out to determine suitable selection factors for recipients and embryos which could improve pregnancy rates following bovine embryo transfer. The experiment included 52 surgical transfers from February, 1985 through June, 1986 performed on Kyungpook Breeding Center in southern Korea. The pregnancy rate was highest when recipients were in estrus within 6 hours before the donor to 12 hours after the donor (78.3% versus 50% for recipients in estrus earlier or later). Pregnancy rates were acceptable following culture under field conditions for up to 17 hours. More recipients over 15 months of age (76.1%) remained pregnant than those under 15 months (66.7%). Embryos transferred during the months from February to July resulted in higher pregnancy rates than those transferred during the remaining 6 months (77.3% versus 57.1%). Transferrable embryos were classified A (best) to C (worst); those graded A or B resulted in significantly higher pregnancy rates than those graded C (81.8% and 73.3% versus 25.0%, p<.05). Pregnancy rates among recipients of the Korean native breed tended to be higher than among Holstein recipients (100% versus 71.1%). Similarly, when the embryo was transferred to the right uterine horn, pregnancy rates tended to be higher than when it was transferred to the left (81.3% versus 65%). Pregnancy rates did not differ according to the stage of development of the embryo; they were for morulae, tight morulae, blastocysts, and advanced blastocysts, respectively: 75.0%, 66.7%, 75.0%, and 77.4%.

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Production of Clone Animals by Nuclear Transplantation I. Effects of Electrostimulation on Membrane Fusion of Embryos and Activation of Oocytes in Mouse (핵치환에 의한 Clone Animal의 생산에 관한 연구 I. 생쥐 수정란의 세포막 융합과 난모세포의 활성화에 미치는 전기자극의 효과)

  • 이상진;구덕본;이상민;박흠대;정순영;정길생
    • Korean Journal of Animal Reproduction
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    • v.18 no.3
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    • pp.217-228
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    • 1994
  • These experiments were carried out to establish the optimal condition of electrostimulatin inducing cell fusion and oocyte activation for nuclear transplantation in mouse embryos. Eggs selected for cell fusion or activation by electrostimulation were equilibrated for 5~10 min. in 0.3M sucrose solution and electrostimulated for 60$\mu$sec using 1 pulse of 60, 70, 80, 90 or 100 volts DC with electrodes 0.2 mm apart. Then they were cultured in 20${mu}ell$ dropsof Tyrode's solution. The results of these experiments are as follows : 1. When one pulse of 60, 70, 80, 90 or 100 volts DC for 60$\mu$sec were applied to 2-cell embryos for fusion of blastomeres, fusion rates were 50.0, 81.7, 91.7, 100 and 100%, respectively ; and developmental rates of fused embryos to blastocyst were 76.7 to 81.5%. Higher fusion rates were observed in 90V and 100V. 2. The average cell number in fused embryos developed to blastocyst was about half of the cell number in diploid controls; and the cell number decreased with increasing of voltages. 3. When pulse numbers were increased, fusion rates improved, but developmental rates were not signficiantly different from the group for which the number of pulse was not increased. And the cell number of blastocyst decreased even more. 4. Oocytes aged for 6hrs after ovulation were electrostimulated for oocyte activation by the same method used for cell fusion. Rates of oocyte activated by electrostimulation were 45.3 to 60.4%, and fragmentation rates were 7.5~15.1%. The lysis rates were 17.0~34.0%. The results of these experiments indicate that the optimal condition for achieving cell fusion and activation is 1 pulse, duration 60$\mu$sec in 90 Volt. The results also show that this condition is suitable for nuclear transplantation using mouse eggs.

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Effects of Elevated $CO_2$ on Maize Growth

  • Kim, Young-Guk;Cho, Young-Son;Seo, Jong-Ho;Kim, Sok-Dong;Shin, Jin-Chul;Park, Ho-Ki
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.1
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    • pp.93-101
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    • 2008
  • The effects of $CO_2$ enrichment on growth of maize (Zea mays L.) were examined. Parameters analyzed include growth characteristics, yields, photosynthetic rates, evaporation rates and photosynthesis-related characteristics under elevated $CO_2$. The plants were grown in growth chambers with a 12-h photoperiod and a day/night temperature of $28/21^{\circ}C$ at the seedling stage and $30/23^{\circ}C$ from the silking stage. The plants were exposed to two elevated $CO_2$ of 500, 700ppm and ambient levels (350 ppm). Chalok 1 and GCB 70 germinated three days after seeding, and germination rates were faster in the elevated $CO_2$ than the control. Germination rates displayed significant differences among the $CO_2$ treatments. At the seedling stage, leaf area, top dry weight, and photosynthetic rates, and plant height indicated positive relationship with elevated $CO_2$ concentrations. At the $5{\sim}6$ leaf stage, $CO_2$ concentration also indicated positive relationship with plant height, leaf area, top dry weight, and photosynthetic rates. At the silking stage, increased plant height of Chalok 1 was noted in the $CO_2$ treatments compared to the control. No significant differences were noted for GCB 70, in which leaf area decreased but photosynthetic rates increased progressively with $CO_2$ concentration. Stomatal aperture was a little bigger in the elevated $CO_2$ than the control. $CO_2$ concentration was negatively related to stomatal conductance and transpiration rates, resulting in high water use efficiency.

A Study of Active Labor Market Policy and Unemployment : An Analysis Using Fuller-Battese Model (적극적 노동시장정책의 실업 감소 효과에 관한 연구)

  • Kang, Chul-Hee;Kim, Kyo-Seong;Kim, Young-Bum
    • Korean Journal of Social Welfare
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    • v.45
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    • pp.7-39
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    • 2001
  • This paper examines the effect of active labor market policy on the unemployment rates in 8 welfare states. This paper focuses on the following questions: what are the major predictors of the changes in unemployment rates?; and what is the effect of active labor market policy in reducing unemployment rates? Using the data from Comparative Welfare States Data Set by Stephens (1997), Key Indicators of the Labour Market by ILO (1999) and Social Expenditure Database by OECD (1999), this paper attempts to answer the above research questions. Fuller-Battese model, a data analysis method in pooled cross-sectional time-series analysis, is adopted to identify variables predicting changes in unemployment rates. This paper analyzes the predictors by using 3 analysis models about 2 types of unemployment (overall unemployment and long term unemployment). Results are as follows: (1) economic variable such as changes in GDP has a positive effect in reducing unemployment rates; (2) active labor market policy has a positive effect in reducing unemployment rates as well; (3) job brokering service among 3 major active labor market programs has a positive effect in reducing unemployment rates; and (4) there is an interaction effect between unemployment benefit level and active labor market policy in reducing unemployment rates. Through the empirical analysis, this paper provides valuable knowledge about effects of active labor market policy on unemployment in 8 welfare states and discusses implications for the active labor market policy in Korea.

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Dose rate measurement of Leksell Gamma Knife Perfexion using a 3D printed plastic scintillation dosimeter

  • Lee, Sangmin;Kim, Tae Hoon;Jeong, Jae Young;Son, Jaebum;Kim, Dong Geon;Cho, Gyu-Seok;Choi, Sang Hyoun;Chung, Hyun-Tai;Kim, Yong Kyun
    • Nuclear Engineering and Technology
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    • v.52 no.10
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    • pp.2334-2338
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    • 2020
  • In recent years, 3D printing technology has received significant research attention. Additionally, 3D printing technology is being applied to study radiation dosimeters of various materials. In this study, a plastic scintillator for 3D printing was developed in a laboratory and used to manufacture a plastic scintillation dosimeter (PSD) with a shape identical to that of the ionization chamber PTW31010. The 16-mm beam of Gamma Knife® Perfexion™ was irradiated to derive the absorbed dose rates of the PSD and PTW31010; they were subsequently compared with the dose rates of the treatment plan. The differences in the dose rates of the Gamma Knife treatment plan and the absorbed dose rates of PTW31010 were within 0.87%. The difference between the dose rates of the Gamma Knife treatment plan and the absorbed dose rates of the PSD were within 4.1%. A linear fit of the absorbed dose rates of four shots involving different dose rates and irradiation angles yielded an adjusted R-square value exceeding 0.9999. A total of 10 repeated measurements were conducted for the same shot to confirm its reproducibility, with a relative error of 0.56%.

Body Surface Area Is Not a Reliable Predictor of Tracheal Tube Size in Children

  • Uzumcugil, Filiz;Celebioglu, Emre Can;Ozkaragoz, Demet Basak;Yilbas, Aysun Ankay;Akca, Basak;Lotfinagsh, Nazgol;Celebioglu, Bilge
    • Clinical and Experimental Otorhinolaryngology
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    • v.11 no.4
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    • pp.301-308
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    • 2018
  • Objectives. The age-based Cole formula has been employed for the estimation of endotracheal tube (ETT) size due to its ease of use, but may not appropriately consider growth rates among children. Child growth is assessed by calculating the body surface area (BSA). The association between the outer diameter of an appropriate uncuffed-endotracheal-tube (ETT-OD) and the BSA values of patients at 24-96 months of age was our primary outcome. Methods. Cole formula, BSA, age, height, weight and ultrasound measurement of subglottic-transverse-diameter were evaluated for correlations with correct uncuffed ETT-OD. The Cole formula, BSA, and ultrasound measurements were analyzed for estimation rates in all patients and age subgroups. The maximum allowed error for the estimation of ETT-OD was ${\leq}0.3mm$. Patients' tracheas were intubated with tubes chosen by Cole formula and correct ETT-OD values were determined using leak test. ETT exchange rates were recorded. Results. One-hundred twenty-seven patients were analyzed for the determination of estimation rates. Thirteen patients aged ${\geq}72months$ were intubated with cuffed ETT-OD of 8.4 mm and were accepted to need uncuffed ETT-OD >8.4 mm in order to be included in estimation rates, but excluded from correlations for size analysis. One-hundred fourteen patients were analyzed for correlations between correct ETT-OD (determined by the leak test) and outcome parameters. Cole formula, ultrasonography, and BSA had similar correct estimation rates. All three parameters had higher underestimation rates as age increased. Conclusion. The Cole formula, BSA, and ultrasonography had similar estimation rates in patients aged ${\geq}24$ to ${\leq}96months$. BSA had a correct estimation rate of 40.2% and may not be reliable in clinical practice to predict uncuffed-ETT-size.

A comparative study of stochastic mortality models considering cohort effects (코호트 효과를 고려한 확률적 사망률 예측 모형의 비교 연구)

  • Kim, Soon-Young
    • The Korean Journal of Applied Statistics
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    • v.34 no.3
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    • pp.347-373
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    • 2021
  • Over the past 50 years, explorative research on the nation's mortality decline patterns has showed a decrease in age-specific mortality rates in all age groups, but there were different improvement patterns in specific mortality rates depending on ages and periods. Greater distinct improvement was observed in mortality rates among women than men, and there was a noticeable improvement in mortality rates in certain groups especially in the more recent decades, revealing a structural change in the overall trends regarding death periods. In this paper, we compare various stochastic mortality models considering cohort effects for mortality projection using Korean female mortality data and further explore the uncertainty related to projection. It also created age-specific mortality rates and life expectancy for women until 2067 based on the results of the analysis, and compared them with future age-specific mortality rates and life expectancy provided by the national statistical office (KOISIS). The best optimal model could vary depending on data usage periods. however, considering the overall fit and predictability, the PLAT model would be regarded to have appropriate predictability in terms of the mortality rates of women in South Korea.

Development of Inspection Checklist through the Analysis of the Relationship between the Risk Improvement Ratio and the Accident Ratio in Food Manufacturing Industry (식료품 제조업 유해·위험요인 개선율과 재해율의 관계 분석을 통한 점검 체크리스트 개발)

  • Ho houng Yu;Doo-Hyun Kim;Sung-Chul Kim
    • Journal of the Korean Society of Safety
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    • v.38 no.4
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    • pp.47-53
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    • 2023
  • In the inspection of workplace hazards/risk factors by specialized institutions dedicated to safety management, inspection reports vary based on the inspectors, who lack the authority to enforce improvement of workplace hazards/risk factors. Thus, improvement and accident rates remain steady without decreasing. This study performed a regression analysis on the relationship between improvement and accident rates of categorized inspection items by classifying hazards/risk factors from inspection reports submitted by a specialized safety management institution in Chungbuk after inspecting 10 food and beverage manufacturers over the past three years. The hazards/risk factors were classified into five categories: mechanical, electrical, chemical, human, and environmental. The regression analysis revealed that the improvement rate of hazards/risk factors inspected by the specialized safety management institution influenced accident rates. To enhance improvement rates based on these findings, this study prioritized the correction of the five most frequently cited inspection items with the lowest improvement rates in each area. Based on these inspection items, this study suggested a checklist for use in workplace safety inspections of food manufacturers. This proposed checklist is expected to reduce accident rates in food manufacturing facilities. Currently, guidance and inspection of workplaces are mainly focused on accident rates rather than correcting hazards/risks. Thus, accident rates remain unchanged as workplace risks are inadequately improved according to the unique characteristics of each workplace. When conducting workplace guidance and inspection, policy measures and inspection methods are warranted to increase the improvement rate of hazards/risks.

Effect of Exposure to Vitrification Solutions on Maturation and Cleavage Rates of Immature Porcine Oocytes in Vitro

  • Park, I. K.;H. B. Song
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.113-113
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    • 2003
  • This study was conducted to investigate the effect of vitrification solution(VS) on in vitro developmental competence of immature porcine oocytes. The immature porcine oocytes were exposed to the following vitrification solution, at RT. 1) EFS sol. : 20% ethylene glycol (EG) 3 min, 40% EG + 18%(w/v) Ficoll(MV70, 000) + 0.3 M sucrose 30 sec, 2) GE sol. : 10% glycerol 5 min, 10% G + 20% EG 5 min, 25% G +25% EG 30 sec, 3) EG sol : 1.5M EG 2.5 min, 5.5 M EG + 1.0 M sucrose 30 sec. Oocytes were immediately transferred into 1.0 M, 0.5 M, 0.25 M, 0125 M, 0 M sucrose solution for 2.5 min each at RT. After removal of VS, immature oocytes were matured in vitro and subsequently all oocytes were subjected to IVF followed in vitro culture for 7 days. Maturation rates of oocytes were 38.8%, 44.5%, 22.4% and 57.6%, in EFS, EG, GE and Control, respectively, maturation rates of oocytes in EG and Control was significantly higher than EFS and GE(P<0.01). Fertilization rates of oocytes in Control was significantly higher than other treated groups(P<0.05), but no difference were observed among treated groups. Polyspermic rates were no significant difference among four groups. Cleavage rates of oocytes were 21.9%, 47.1%, 19.0% and 65.9%, in EFS, EG, GE and Control, respectively, cleavage rates of oocytes in EG and Control was significantly higher than EFS and GE(P<0.05), but blastocyst formation rates were no significant difference among four groups. These results suggested that the use of EG solution could be a great challenge for reaching a successful vitrification of immature porcine oocytes.

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Trend of Smoking and Second-Hand Smoke Rate: Year 2015-2020 (연도별 흡연율 및 간접흡연율 추이 분석: 2015-2020)

  • Ji-Woong Nam;Kook-Young Jang;Gi-Hyun Kim;Chung-Nyun Kim;Ji-Soo Song;Jin-Won Noh;Ki-Bong Yoo
    • Health Policy and Management
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    • v.33 no.1
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    • pp.85-106
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    • 2023
  • Background: The Korean government has established Health Plan and reinforced tobacco control policies step by step according to Framework Convention on Tobacco Control (FCTC). This study aims to investigate yearly smoking and secondhand smoking (SHS) exposure rates adjusted by demographic and socioeconomic factors. Methods: Multiple logistic regression analysis was conducted about the smoking experience, current smoking, and exposure to SHS at home, at work, and in public places using data from the 6th to 8th Korea National Health and Nutrition Examination Survey (2015-2020). Results: Comparing 2015 with other years, smoking experience rates significantly decreased from 2015 to 2018 in men and significantly increased from 2018 to 2020 in women. Compared to 2015, current smoking rates significantly decreased only in 2020 for men and significantly increased in 2018 and 2019 for women. The rate of exposure to SHS at home significantly decreased until 2018. Rates of exposure to SHS in the workplace and public places were significant all year. Conclusion: There were a slowly decreasing trend in men and an increasing trend in women at current smoking rates. It was found that there were significant decreasing trends at exposure to SHS. There is a need to set policies that reduce current smoking rates and especially to set a customized program to lower women's smoking rates.