• Title/Summary/Keyword: rate of strain

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Photoproduction of Hydrogen from Acetate by Rhodopseudomonas: Effect of Culture Conditions and Sequential Dark/Light Fermentation

  • Oh, You-Kwan;Seol, Eun-Hee;Park, Sung-Hoon
    • 한국생물공학회:학술대회논문집
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    • 2003.10a
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    • pp.422-427
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    • 2003
  • Rhodopseudomonas palustris P4 can produce $H_2$ either from CO by water-gas shift reaction or from various sugars by anaerobic fermentation. Fermentative $H_2$ production by P4 is fast, but its yield is relatively low due to the formation of various organic acids. In order to increase $H_2$ production yield from glucose, P4 was investigated for the photo-fermentation of acetate which is a major by-product of fermentative $H_2$ production. Experiments were performed in batch modes using both light-grown and dark-grown cells. When the dark-grown P4 was challenged with light and acetate, $H_2$ was produced with the consumption of acetate after a lag period of 25 h. $H_2$ production was inhibited when a nitrogen source, especially ammonium, is present. When the dark-fermentation broth containing acetate was adopted for photo-fermentation with light-grown cells, $H_2$ production and concomitant acetate consumption occurred without a lag period. The $H_2$ yield was estimated as 2.4 - 2.8 mol $H_2/mol$ acetate and the specific $H_2$ production rate was as 9.8 ml $H_2/g$ cell${\cdot}$h, The fact that a single strain can perform both dark- and light-fermentation gives a great advantage in process development Compared to a one-step dark-fermentation, the combined dark- and light-fermentation can increase the $H_2$ production yield on glucose by two-fold.

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Electrochemical Properties of LiNiyMn2-yO4 Prepared by the Solid-state Reaction

  • Song, Myoung-Youp;Kwon, Ik-Hyun;Shon, Mi-Suk
    • Journal of the Korean Ceramic Society
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    • v.40 no.5
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    • pp.401-404
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    • 2003
  • LiN $i_{y}$M $n_{2-y}$ $O_4$were synthesized by calcining a mixture of LiOH, Mn $O_2$(CMD), and NiO at 40$0^{\circ}C$ for 10 h and then calcining at 85$0^{\circ}C$ for 48 h in air with intermediate grinding. The voltage vs. discharge capacity curves at a current density 300 $\mu$A/c $m^2$ between 3.5 V and 4.3 V showed two plateaus, but the plateaus became ambiguous as the y value increases. The sample with y=0.02 had the largest first discharge capacity, 118.1 mAh/g. As the value y increases from 0.02 up to 0.2, on the whole, the cycling performance became better. The LiN $i_{0.10}$M $n_{1.90}$ $O_4$sample had a relatively large first discharge capacity 95.0 mAh/g and showed an excellent cycling performance. The samples with larger lattice parameter have, in general, larger discharge capacities. The reduction curves in the cyclic voltammograms for the y=0.05-0.20 samples exhibit three peak showing that the reduction may proceed in three stages in these samples. For the samples with relatively large discharge capacity, the lattice destruction induced by strain causes the capacity fading of LiN $i_{y}$M $n_{2-y}$ $O_4$ with cycling.cling.ing.

Isolation and Characterization of a Mutant Defective in Light-activated Heterotrophic Groth from Synechocystis sp. PCC 6803 (Synechocystis sp. PCC 6803으로부터 광활성 종속영양 생장결핍 돌연변이체의 분리 및 특성)

  • Park, Mi-Seon;Lee, Young-Sook;Kim, Young-Chang
    • Korean Journal of Microbiology
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    • v.32 no.3
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    • pp.202-207
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    • 1994
  • A mutant strain PRM1 defective in light-activated heterotrophic growth was isolated from Synechocystis sp. PCC 6803. PRM1 could be grown at growth rate equivalent to Synechocystis 6803 under mixotrophic growth conditions. However, PRM1 could not be grown under light-activated heterotrophic conditions, in which a daily pulse of light for 5 min was given. These results suggest that PRM1 is not defective in heterotrophic metabolism, but in the transduction pathway of light signal essential to the growth. Plasmid patterns, absorption spectra of whole cells, and the exterior and interior structures of PRM1 were similar to those of Synechocystis 6803, except that PRM1 could not produce amorphous slime holding cells together.

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From food wastes into useful probiotics: Development of Lactic acid bacteria as useful probiotics for animal feed without antibiotics using food waste (음식물류폐기물의 자원화를 위한 항세균 활성 유산균의 개발)

  • Seo, Jeong-Yong;Song, In-Geun;Lee, Dae-Gyu;Lee, Ki-Young;Kim, Young-Jun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.14 no.2
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    • pp.112-120
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    • 2006
  • For the conversion of food waste into a good recycling material such as animal feed without antibiotics, thirteen lactic acid bacteria, which can be used as good probiotics for animal feed, were isolated from the intestine and feces in pigs. All isolates showed strong tolerance to high salt (4% of NaCl), acid(pH4.0), and bile juice (0.8% of oxgall). The growth rate was best at $37^{\circ}C$ in all strains. Among the isolates, Lactobacillus plantarum CJY-22, L. brevis CJY-42, L. arizonensis CJY-3, and Pediococcus sp. CJY-41 showed higher and broader spectrum of antimicrobial activities against six different pathogens such as Salmonella, typhimurium. L. plantarum CJY-22 has also grown well at $25^{\circ}C$, making this strain as an appropriate candidate for the fermentation of food waste at room temperature, thus conducting the fermentation process cost-effectively.

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A Study on the Transition of Hydrogen-Air and LPG-Air Explosion to Fire (수소와 액화석유 가스의 공기혼합기의 폭발 후 화재로 전이 연구)

  • Oh Kyu-Hyung;Lee Sung-Eun;Rhie Kwang-Won
    • Journal of the Korean Society of Safety
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    • v.19 no.4 s.68
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    • pp.150-154
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    • 2004
  • Gas explosion characteristics of hydrogen and liquefied petroleum gas(LPG) were measured in 6L cylindrical vessel, and experiment for explosion to fire transition phenomena of the gases were carried out using the 270L vessel. Explosion characteristics were measured using the stain type pressure transducer and explosion to fire transition phenomena was analyzed with the hish-speed camera. Base on the experiment, it was found that explosion pressure was most high slightly above the stoichiometric concentration, and explosion pressure rise rate and flame propagation velocity were proportional to the combustion velocity. And we find that those kind of explosion characteristics affect the explosion-to-fire transition, in addition, explosion flame temperature, flame residence time, are important parameters in explosion-to-fire transition.

Growth and Excretion of Nitrogen and Phosphorus of Israeli Strain of Carp(Cyprinus carpio) Fed a Low Pollution Diets (저오염 사료의 급여에 의한 잉어의 성장과 질소 및 인 배설량)

  • 김정대;이종윤;김광석;이승복;최낙중;김응오
    • Journal of Aquaculture
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    • v.11 no.1
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    • pp.39-48
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    • 1998
  • Growth performance and excretions of nitrogen (N) and phosphorus (P) were investigated with carp (Cyprinus carpio) with carp (Cyprinus carpio) grower fed a low pollution diets (A and B) and commercial ones (Com-1 and Com-2). A recirculated rearing system (Exp. I) and a floating net cage system (Exp. II) were employed for two feeding trials in which fish having an initial body weight of 152g and 193g were fed for 41 an 39 days, respectively. The highest weight gain, daily growth rate and protein efficiency ratio were found (P<0.05) in fish fed diet A containing 10% fish meal and 2% monocalcium phosphate (MCP) for both experiments. They also showed the lowest feed conversion ratio and daily feeding ration among treatments. Fish fed diet B containing 10% fish meal, 5% fish protein concentrate and 1% MCP showed weight gain and FCR more improved (P<0.05) than those fed commercial diets. In all groups, whole body cmpositions were not greatly different among treatment, and protein and P contents in final fish ranged from 14.3 to 15.6% and from 0.39 to 0.48%, respectively. Fish fed diet A excreted the least N which were 38.3 in Exp. I and 39.6g/kg gain in Exp. II. However, the values found in fish fed two commercial diets amounted to 59.1 and 58.9g, respectively. A significant decrease in P excretion was also found in fish groups fed diets A and B. In Exp. I, a reduction of 53.4% was shown in fish fed diet A, compared to the averaged value (18.5g P/kg gain) of two commercial groups. In Exp. II conducted using the floating net cages, fish fed diet B excreted the least P (8.6g/kg gain) among the treatment, which was followed by fish fed diet A, showing 48.6% reduction compared to the average value (18.5g/kg gain) for fish fed two commercial diets. The present results clearly showed that N and P excretions from fish culture could be significantly reduced by using the low pollution diet.

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Transition from Cycle-Dependent to Time-Dependent Fatigue Crack Propagation at Creep Temperature of SUS 304 Steel (SUS 304鋼 의 크리이프 溫度領域 에 관한 時間依存型 및 사이클依存型 疲勞크랙 傳播 의 遷移)

  • 유헌일;주원식
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.9 no.5
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    • pp.539-547
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    • 1985
  • The low-cycle fatigue crack growth behavior of SUS 304 Stainless steel was investigated at 650.deg. C by the nonlinear fracture mechanics. Crack Propagation can be separated in to cycle-dependent and time-dependent, the former is correlated with .DELTA. $J_{f}$ , J-intergral range and the latter is correlated with J', modified J integral. Transition from cycle-dependent to time-dependent crack growth was successfully predicted using the .betha. hypothesis, which was proposed by the authors on the basis of an analysis on the interaction of elastic and creep strain. To investigate the reliability of .betha.-hypothesis, experimenting by the change of stress-level, stress rate and frequency, following conclusions were obtained. (1) High temperature fatigue crack propagation was separated into cycle-dependent and time-dependent. (2) Transition of crack propagation was predicted by .DELTA. $J_{c}$/.DELTA.$_{f}$ or .betha. (3) Lower limit in cycle-dependent crack propagation was obtained..

High Temperature Deformation Behaviour of Particulate Reinforced Aluminium Composites (입자분산강화 알루미늄 복합재료의 고온거동에 관한 연구)

  • Gwon, Hyeok-Cheon;Yun, Ui-Park
    • Korean Journal of Materials Research
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    • v.5 no.7
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    • pp.765-774
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    • 1995
  • The hot deformation behaviour of particulate reinforced aluminium 6061 Al composite were investigated by hot compression tests in the temperature range from 623K to 823K with strain rate of 10$^{-3}$ ~5.0 S$^{-1}$ . The effect of reinforced particulate volume fraction, mean diameter on the high temperature flow stress has also been studied. Experimental results showed that the increase in the volume fraction of reinforcement contributed to the rising of yield stress, but the stress above the yield point appeared to be steady state at all volume fractions. The apparent activation energy for deformation was 290KJ/mo1 for unreinforced 6061 Al, 327KJ/mo1 for 6061 Al-20vo1.% SiC composite and 531KJ/mo1 for 6061 Al-20vo1.%A1$_2$O$_3$composite. It appeared that $Al_2$O$_3$reinforced composites was more difficult to hot deform.

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Characteristics of Negative Skin Friction of Foundation Pile and Construction Management by Experimental Field Test (현장시험을 통한 기초 말뚝 부마찰력의 특성과 시공관리)

  • Hong, Seok-Woo
    • International Journal of Highway Engineering
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    • v.14 no.3
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    • pp.41-48
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    • 2012
  • In this study the negative skin friction test of foundation pile was performed in order to monitor the negative skin frictional force acting on the steel pipe pile installed in soft soil. The monitored frictional stresses obtained from the long-term loading test. Through the long-term frictional stress monitoring test, the economical period for the construction of the superstructure was determined. The following conclusion were derived from this study: (1) In soft soil, negative skin friction increases with the increase in the rate of settlement. (2) In the friction relationship graph, the period where there is no frictional strain increase is verified and the time for the construction of the superstructure is determined. (3) The pile loading test was performed and the negative skin friction was compared with the test results. It was determined that the negative skin friction after driving was larger than the negative skin friction obtained from the loading test. 15 days after the construction, the monitored value was similar with the theoretical data. (4) It was determined that even during the occurrence of negative skin friction an economical construction management can be performed using the long-term monitoring method of negative skin friction.

Wedge Splitting Test and Fracture Energy on Particulate Reinforced Composites (입자강화 복합재료의 쐐기분열시험 및 파괴에너지 평가)

  • Na, Seong Hyeon;Kim, Jae Hoon;Choi, Hoon Seok;Park, Jae Beom;Kim, Shin Hoe;Jung, Gyoo Dong
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.40 no.3
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    • pp.253-258
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    • 2016
  • The effect of temperature on the fracture energy, crack propagation, and crack tip opening displacement (CTOD) was determined for particulate reinforced composites using the wedge splitting test. The materials that were used consisted of a polymer binder, an oxidizing agent, and aluminum particles. The test rate of the wedge splitting specimen was 50 mm/min, the temperature conditions were $50^{\circ}C$, room temperature, $-40^{\circ}C$, and $-60^{\circ}C$. The fracture energy, calculated from splitting load-crack mouth opening displacement(CMOD) curves, increased with decreasing temperature from $50^{\circ}C$ to $-40^{\circ}C$. In addition, the strength of the particulate reinforced composites increased sharply at $-60^{\circ}C$, and the composites evidenced brittle fracture due to the glass transition temperature. The strain fields near the crack tip were analyzed using digital image correlation.