• Title/Summary/Keyword: rate of strain

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Alkaline $\alpha$-amylase Production from Bacillus megaterium

  • Jia, Shiru;Lim, Chae-kyu;Seo, Gwang-Yeob;Nam, Hyung-Gun
    • Journal of environmental and Sanitary engineering
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    • v.24 no.1
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    • pp.40-46
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    • 2009
  • The enzyme expressed from strain L-49 was 2.01 times higher than that of original strain. Strain L-49 can grow on culture plate with $50{\mu}g/mL$ ampicillin. The synthesis of $\alpha$-amylase was greatly suppressed when strain L-49 was grown on monosaccharide such as glucose and polysaccharide at the same time cell concentration was low. Amylase production was enhanced when the bacterium was grown on starch and dextrin. Among different nitrogen sources tried, yeast extract was found to be the best followed by panpeptone, peptone, meat extract, bean meal, and corn steep liquor. The average rate of enzyme production was enhanced for 3~4 times in fermentation time from 24h to 44h. The sugar uptake rate has also increased. Low oxygen supply rate enhanced the rate of strain propagation but depressed the enzyme production. Hence it is benefit to obtain high enzyme activity that agitation speed maintained not lower than 400r/min and aeration rate maintained greater than 1:1vvm.

Effect of Hydrocarbon Uptake Modes on Oil Degradation Rate by Mixed Cultures of Petroleum Degraders (Hydrocarbon Uptake Modes에 따른 유류분해 미생물 혼합체의 원유분해능)

  • 고성환;이홍금;김상진
    • KSBB Journal
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    • v.13 no.5
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    • pp.606-614
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    • 1998
  • In this study, biodegradation rate of Arabian light crude oil by mixed cultures of selected petroleum-degraders was determined. Their modes of hydrocarbon uptake were then observed to determine whether there are differences in biodegradation rate by the mixed cultures. By the mixed cultures of petroleum-degraders having same modes of hydrocarbon uptake, such as strain US1 and K1 (using pseudo-solubilized hydrocarbons by a biosurfactants), K2-2 and P1(using hydrocarbons by direct contact), CL 180 and IC-10 (mixed type of uptake modes), the biodegradation rates of aliphatics in the crude oil were increased more than those by their pure cultures, about 40%, 25% and 20%, respectively. Biodegradation rate of strain KH3-2 (using only water- dissolved hydrocarbons) was increased by mixed cultures with strain K1, CL180 or IC-10 possessing high emulsifying activity. However, the biodegradation rate of the crude oil was decreased about 20%-40% by the mixed cultures of petroleum-degraders having different mode of hydrocarbon uptake, such as addition of strain US1 or K1 in the cultures of K2-2 or P1. Biosurfactants produced by US1 or K1 seems to enhance the emulsification of crude oil in aqueous phase but inhibit the attachment of K2-2 or P1 to crude oil. As same phenomena, the addition to Triton X-100 into the culture of strain US1, K1, CL180, IC-10 or KH3-2 increased the biodegradation rate, but the addition in the culture of strain K2-2 or P1 decreased the biodegradation rate. The mixed culture made of CL180, IC-10 and KH3-2 degraded 61.5% of aliphatics and 69% of aromatics in 3% (v/v) of Arabian light crude oil added.

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Analysis of the dynamic confining effect of CRAC short column under monotonic loadings

  • Wang, Changqing;Xiao, Jianzhuang
    • Structural Engineering and Mechanics
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    • v.74 no.3
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    • pp.351-363
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    • 2020
  • Based on the dynamic tests of recycled aggregate concrete (RAC) short columns confined by the hoop reinforcement, the dynamic failure mechanism and the mechanical parameters related to the constitutive relation of confined recycled aggregate concrete (CRAC) were investigated thoroughly. The fracturing sections were relatively flat and smooth at higher strain rates rather than those at a quasi-static strain rate. With the increasing stirrup volume ratio, the crack mode is transited from splitting crack to slipping crack constrained with large transverse confinement. The compressive peak stress, peak strain, and ultimate strain increase with the increase of stirrup volume ratio, as well as the increasing strain rate. The dynamic confining increase factors of the compressive peak stress, peak strain, and ultimate strain increase by about 33%, 39%, and 103% when the volume ratio of hoop reinforcement is increased from 0 to 2%, but decrease by about 3.7%, 4.2%, and 9.1% when the stirrup spacing is increased from 20mm to 60mm, respectively. This sentence is rephrased as follows: When the stirrup volume ratios are up to 0.675%, and 2%, the contributions of the hoop confinement effect to the dynamic confining increase factors of the compressive peak strain and the compressive peak stress are greater than those of the strain rate effect, respectively. The dynamic confining increase factor (DCIF) models of the compressive peak stress, peak strain, and ultimate strain of CRAC are proposed in the paper. Through the confinement of the hoop reinforcement, the ductility of RAC, which is generally slightly lower than that of NAC, is significantly improved.

Numerical Study on Dynamic Behavior of Diffusive-Thermal Instability in $CH_4/O_2$ Conterflow Diffusion Flames (메탄/산소 대향류 확산화염에서 확산-열 불안정으로 인한 화염의 거동에 관한 수치적 연구)

  • Sohn, Chae-Hoon
    • 한국연소학회:학술대회논문집
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    • 2004.06a
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    • pp.95-101
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    • 2004
  • Dynamic behavior of diffusive-thermal instability in diluted $CH_4/O_2$ diffusion flames is numerically investigated by adopting detailed chemistry and transport. Counterflow diffusion flame is adopted as a model flamelet. Particular attention is focused on the pulsating-instability regime, which arises for Lewis numbers greater than unity, and the instability occurs at high strain rate near extinction condition in this flame configuration. Once a steady flame structure is obtained for a prescribed value of initial strain rate. transient solution of the flame is calculated after a finite amount of strain-rate perturbation is imposed Oil the steady flame. Transient evolution of the flame depends on the initial strain rate and the amount of perturbed strain rate. Basically, the dynamic behaviors can be classified into two types, namely non-oscillatory decaying solution and diverging solution leading to extinction. The peculiar oscillatory solution. which has been found in the previous study adopting one-step chemistry and constant Lewis numbers, is not observed in this study, which is attributed to both convective flow and preferential diffusion effects.

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Oscillatory Instability of Low Strain Rate Edge Flame (저신장율 에지 화염의 진동 불안정성)

  • Kim Kang-Tae;Park June-Sung;Kim Jeong-Soo;Oh Chang-Bo;Keel Sang-In;Park Jeong
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.30 no.4 s.247
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    • pp.343-349
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    • 2006
  • Systematic experiments in $CH_4/Air$ counterflow diffusion flames diluted with He have been undertaken to study the oscillatory instability in which lateral flame size was less than burner nozzle diameter and thus lateral heat loss could be remarkable at low global strain rate. The oscillatory instability arises for Lewis numbers greater than unity and occurs near extinction condition. The oscillation is the direct outcome from the advancing and retreating edge flame. The dynamic behaviors of extinction in this configuration can be classified into three modes; growing, harmonic and decaying oscillation mode near extinction. As the global strain rate decreases, the amplitude of the oscillation becomes larger. This is caused by the increase of lateral heat loss which can be confirmed by the reduction of lateral flame size. Oscillatory edge flame instabilities at low global strain rate are shown to be closely associated with not only Lewis number but also heat loss (radiation and lateral heat loss).

Fatigue characteristics of distributed sensing cables under low cycle elongation

  • Zhang, Dan;Wang, Jiacheng;li, Bo;Shi, Bin
    • Smart Structures and Systems
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    • v.18 no.6
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    • pp.1203-1215
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    • 2016
  • When strain sensing cables are under long-term stress and cyclic loading, creep may occur in the jacket material and each layer of the cable structure may slide relative to other layers, causing fatigue in the cables. This study proposes a device for testing the fatigue characteristics of three types of cables operating under different conditions to establish a decay model for observing the patterns of strain decay. The fatigue characteristics of cables encased in polyurethane (PU), GFRP-reinforced, and wire rope-reinforced jackets were compared. The findings are outlined as follows. The cable strain decayed exponentially, and the decay process involved quick decay, slow decay, and stabilization stages. Moreover, the strain decay increased with the initial strain and tensile frequency. The shorter the unstrained period was, the more similar the initial strain levels of the strain decay curves were to the stabilized strain levels of the first cyclic elongation. As the unstrained period increased, the initial strain levels of the strain decay curves approached those of the first cyclic elongation. The tested sensing cables differed in the amount and rate of strain decay. The wire rope-reinforced cable exhibited the smallest amount and rate of decay, whereas the GFRP-reinforced cable demonstrated the largest.

A Study on the Resistance and Crack Propagation of ITO/PET Sheet with 20 nm Thick ITO Film (20 nm 두께의 ITO층이 코팅된 ITO/PET Sheet의 저항 및 균열형성 특성 연구)

  • Kim, Jin-Yeol;Hong, Sun-Ig
    • Journal of the Korean Ceramic Society
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    • v.46 no.1
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    • pp.86-93
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    • 2009
  • The crack formation and the resistance of ITO film on PET substrate with a thickness of 20 nm were investigated as a function of strain. The onset strain for the increase of resistance increased with increasing strain rate, suggesting the crack initiation is dependent on the strain rate. Electrical resistance increased at the strain of 1.6% at the strain rates below $10^{-4}/sec$ while it increased at ${\sim}2%$ at the strain rates above $10^{-3}/sec$. The critical strain at which the cracks were formed is close to the proportional limit. Upon loading, the initial cracks perpendicular to the tensile axis were observed and propagated the whole sample width with increasing strain. The spacing between horizontal cracks is thought to be determined by the fracture strength and the interfacial strength between ITO and PET. The crack density increased with increasing strain. However, the effect of the strain rate on the crack density was less pronounced in ITO/PET with 20 nm ITO thickness than ITO/PET with 125 nm ITO thickness, the strength of ITO film is thought to increase as the thickness on ITO film decreases. The absence of cracks on ITO film at a strain as close as 1.5% can be attributed to the compressive residual stress of ITO film which was developed during cooling after the coating process. The higher critical strain for the onset of the resistance increase and the crack initiation of ITO/PET with a thinner ITO film (20 nm) can be linked with the higher strength of the thinner ITO film.

A Study on the Microstructures and High Temperature Tensile Properties of Ni-base Superalloy Melt-Spun Ribbons (Ni 기 초합금 급냉응고 리본의 미세구조와 고온 인장특성에 관한 연구)

  • Han, Chang-Suk
    • Journal of the Korean Society for Heat Treatment
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    • v.27 no.4
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    • pp.180-184
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    • 2014
  • In order to make clear relationship between high temperature tensile properties and fine microstructure of rapidly solidified cast-type Ni-base superalloys without heat treatment required for consolidation process, tensile test was carried out by changing strain rate from $5{\times}10^{-5}s^{-1}$ to $2{\times}10^{-2}s^{-1}$ and test temperature from $900^{\circ}C$ to $1050^{\circ}C$ using IN738LC and Rene'80 melt-spinning ribbons by twin roll process which were superior to ribbons by single roll process from the viewpoint of structure homogeneity. The dependence of tensile strength on strain rate and test temperature was studied and strain rate sensitivity, m, were estimated from tensile test results. From this study, it was found that tensile strength was influenced by ${\gamma}^{\prime}$ particle diameter, test temperature and strain rate, and m of ribbons exhibited above 0.3 over $950^{\circ}C$.

Dynamic deformation behavior of rubber under high strain rate compressive loading (플라스틱 SHPB를 사용한 고무의 고변형률 하중하에서의 동적변형 거동)

  • 이억섭;김경준
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2002.10a
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    • pp.849-853
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    • 2002
  • A specific experimental method, the split Hopkinson pressure bar (SHPB) technique has been widely used to determine the dynamic material properties under the impact compressive loading conditions with strain-rate of the order of 103/s~104/s. In this paper, dynamic deformation behaviors of rubber materials widely used for the isolation of vibration from varying structures under dynamic loading are determined using a Split Hopkinson Pressure Bar technique.

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A Study of Consolidation Characteristics of Constant Rate of Strain Consolidation tests with Rates of Strain (변형률속도에 따른 일정변형률시험의 압밀특성 비교연구)

  • 장병욱;차경섭;원정윤
    • Proceedings of the Korean Society of Agricultural Engineers Conference
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    • 1998.10a
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    • pp.455-460
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    • 1998
  • This study performed to establish tile characteristics of constant rate of strain consolidation(CRSC) tests. The values obtained by the CRCS tests were compared with oedometer tests. From the comparision of the results of oedometer and CRSC tests for soft soil from Haenam, Korea, it was concluded that. 1. When rate of strain is 0.9mm/hr, compression curve of CRSC test was not coincided with those of tile oedometer test, then preconsolidation stress was larger than those of other rates of strain. 2. Permeabilities from CRSC tests and direct measurements were about same each other, but permeabilities from Oedometer tests were large than those from others.

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