• 제목/요약/키워드: rat plasma

검색결과 740건 처리시간 0.03초

Identification of IY81149 and Its Metabolites in the Rat Plasma Using the On-Line HPLC/ESI Mass Spectrometry

  • Myung, Seung-Woon;Min, Hye-Ki;Jin, Chang-Bae;Kim, Myung-Soo;Lee, Seung-Mok;Chung, Gi-Ju;Park, Seong-Jun;Kim, Dong-Yeon;Cho, Hyun-Woo
    • Archives of Pharmacal Research
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    • 제22권2호
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    • pp.189-193
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    • 1999
  • Reversed-phase high-performance liquid chromatography/mass spectrometry (HPLC/MS) with an eletcrospray ionization (ESI) interface was applied to the identification of metabolites of IY81149 in the rat plasma. Fragments obtained using collision-induced dissociation (CID) in both positive and negative modes were utilized to elucidate the structure of metabolites. The eluent from the conventional HPLC column was split and directly introduced into an ESI-mass spectrometer for the identification of the structures. the CID technique allowed the sensitive identification of sulfonyl-IY81149 and hydroxy-IY81149 from the rat plasma.

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태음조위탕(太陰調胃湯) 약침(藥鍼)이 고지방 급여 흰쥐의 혈장 및 간장의 지질구성과 항산화능에 미치는 영향 (Effects of taeeumjoweetang Herbal-Acupuncture on plasma and liver lipid composition and antioxidative capacity in rat fed high fat diet)

  • 임윤택;이준무
    • Korean Journal of Acupuncture
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    • 제22권2호
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    • pp.151-161
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    • 2005
  • Objectives : In order to measure the Effects of taeeumjoweetang Herbal- Acupuncture at joksamri(ST36) and kwanweun(CV4) on liver and plasma lipid composition and antioxidative capacity in rat fed high fat diet. Results : Concentrations of plasma triglyceride, total cholesterol and LDL-cholesterol showed a tendency to increase in the high fat diet group. However Herbal-Acupuncture groups showed a lower values than control groups. HDL-cholesterol showed a tendensy to decrease in high 131 fat diet groups and in high fat diet groups, these values showed no significantly different. Liver total cholesterol values showed no significantly different in all treatment groups. Triglyceride concentration showed a high value in control group and other treatment groups showed no significantly different. plasma GOT and GPT values showed a tendency to increase in high fat diet group. however these values decreased in Herbal-Acupuncture group. The concentration of TBARS in liver and plasma showed a high values in high fat diet group, however these values showed a tendency to decrease in aqua- acupuncture group. Glutathione peroxidase, superoxide dismutase and catalase activity values showed a low values in high fat diet group, however these values showed a tendency to increase in Herbal-Acupuncture group.

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Recombinant human erythropoietin (DA-3285)의 실험동물에서의 약동력학 및 조직분포 (Pharmacokinetics and Tissue Distribution of Recombinant Human Erythropoietin (DA-3285) in the Laboratory Animals)

  • 심현주;이응두;이종진;김흥재;이상득;이성희;김원배;양중익
    • Biomolecules & Therapeutics
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    • 제4권1호
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    • pp.78-83
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    • 1996
  • The pharmacokinetics and tissue distribution of DA-3285 (recombinant human erythropoietin, recently manufactured by Research Laboratories of Dong-A Pharmaceutical Company) were studied in the laboratory animals. The plasma, urine, and tissue concentration of DA-3285 were measured by a double-antibody sandwich enzyme immunoassay. After intravenous administration of DA-3285, 20, 100, 500 and 2500 units/kg to rats, the plasma concentrations declined polyexponentially with the terminal half-lives of 2.15, 2.10, 2.31, and 2.35 hr, respectively. Total body clearance (20.7∼26.6 mι/hr/kg) and apparent volume of distribution at steady state (57.2∼70.1 mι/kg) were independent of the dose and AUC increased proportionally with the dose. The renal clearance was much lower than total body clearance, suggesting that extrarenal clearance, presumably metabolism , plays a significant role in elimination of DA-3285. In all rat tissues, the tissue to plasma ratios were smaller than unity, indicating less affinity of DA-3285 to rat tissues and was proved by considerably less value of Vdss. After 3 times a week for consecutive 3 weeks i.v. administration of DA-3285, 100 units/kg to rats, the plasma concentrations and pharmacokinetic parameters of DA-3285 were not significantly different from those in a single administration. After s.c. administration to the rat, plasma concentrations of DA-3285 peaked at 6 hr and the extent of bioavailability was 26.7%. In mice, rabbits and dogs, at DA-3285 dose of 100 units/kg, the mean terminal haw-lives were 2.78, 3.05, and 4.01 hr, respectively. Compared with reported data in the literatures, DA-3285 has similar properties to rh-EPO manufactured by other companies in view of pharmacokinetics.

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Determination of S- and R-Amlodipine in Rat Plasma using LC-MS/MS After Oral Administration of S-Amlodipine and Racemic Amlodipine

  • Yoo, Hye-Hyun;Kim, Tae-Kon;Lee, Bong-Yong;Kim, Dong-Hyun
    • Mass Spectrometry Letters
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    • 제2권4호
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    • pp.88-91
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    • 2011
  • The pharmacokinetic properties of S-amlodipine were studied using racemic amlodipine and single S-enantiomer (SK310) administration to rats. Plasma levels of the drug were determined using chiral liquid chromatography coupled with tandem mass spectrometry following solid phase extraction. The stereospecific analysis of amlodipine was performed on an ${\alpha}$-acid glycoprotein (AGP) column using a mobile phase comprising 10 mM ammonium acetate (pH 4.0) and propanol at a flow rate of 0.2 mL/min. This method was used to perform a comparative study of the pharmacokinetics of amlodipine and SK310. The results revealed that the pharmacokinetic profile of S-amlodipine after the administration of SK310 was comparable to that following the administration of the racemic mixture.

뽕잎추출물이 Zucker Rat의 체지방 축적에 미치는 효과 (The Effect of Mulberry-Leaf Extract on the Body Fat Accumulation in Obese fa/fa Male Zucker Rats)

  • 김순경;김선여;김휘준;김애정
    • 한국식품영양과학회지
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    • 제30권3호
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    • pp.516-520
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    • 2001
  • This study was conducted to investigate whether the obesity of obese fa/fa male Zucker rats can be ameliorated by mulberry leaves. There was no significant difference between mulberry treatment group and control group in body weight gain and food efficiency ratio. It also showed decreases on plasma triglyceride and total-cholesterol concentration compared to the control group. But there was no change in plasma leptin concentration between the control and mulberry treatment groups. These results suggest that mulberry leaves reduce serum lipid levels, but do not effect the plasma leptin concentration.

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LC/MS/MS를 이용한 혈장 중 리도카인의 정량 및 리도카인 패치제의 약물동태학적 평가 (LC/MS/MS Method for the Determination of Lidocaine in Hairless Rat Plasma and Pharmacokinetic Applications of Lidocaine Patch)

  • 황지연;김철준;신영희
    • 약학회지
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    • 제56권3호
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    • pp.158-163
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    • 2012
  • Lidocaine has been studied for many chronic pain conditions, including postherpetic neuralgia (PHN) and recently it has also been increasingly used in transdermal drug delivery systems. In this study, pharmacokinetics of a lidocaine patch was studied in four hairless male rats. The plasma concentration was determined by a validated LC/MS/MS method after applying a $3{\times}2cm^2$ (30mg) patch for 12 hours. From the plasma lidocaine concentration vs time curves, $AUC_{0-20h}$, Cmax, and Tmax of lidocaine patch were $2,926.32{\pm}335.28ng{\cdot}h/ml$, $256.86{\pm}29.63ng/ml$, and $6.00{\pm}2.31h$, respectively.

Loxoprofen sodium 플라스타의 소염, 진통 작용 및 혈중 약물 농도에 대한 연구 (Anti-inflammatory and Analgesic Activities, and Plasma Concentration of Loxoprofen Sodium Plasters)

  • 채주병;전홍렬;이승목;정남주;김수균;조길도;김동연
    • Biomolecules & Therapeutics
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    • 제7권2호
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    • pp.198-203
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    • 1999
  • Loxoprofen-Na (sodium 2-〔4-(2-oxocyclopentylmethyl)pheny)propionate dihydrate) is a potent analgesic drug. We developed loxoprofen-Na plasters to extend duration time of analgesic activity and to reduce side effect on gastrointestinal tract. Analgesic effect of Loxoprofen-Na plasters was investigated. Loxoprofen-Na plaster had good analgesic effect in rat paw pressure test, Tail-flick latency test and acetic acid-induced writhing test. Also, it had anti-inflammatory effect on carrageenan-induced rat hind paw edema. In pharmacokinetic study of Loxoprofen-Na, plasters dosage form showed that plasma drug concentration was prolonged up to 14 hours. So, we can conclude that loxoprofen-Na plasters, when applied on skin, will be a new type of drug for controlling the various local pain or inflammation.

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Characterization of the ${\cdot}O_{2}^{-}$-Formation by Pyridine Nucleotide in Rat Hepatocytes

  • Kim, Ki-Sung
    • BMB Reports
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    • 제28권6호
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    • pp.533-537
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    • 1995
  • The detection with lucigenin under physiological conditions is selective for ${\cdot} O_{2}^{-}$, for it can be accepted that lucigenin indicates actual intramembranal $\cdot O_{2}^{-}- formation$. Lucigenin chemiluminescence (CL) was elicited from the plasma membrane (PM) only by addition of reduced pyridine nucleotide. NADPH was preferred to NADH in PM and hepatocytes. This specificity was masked by $NAD(P)^+$ inhibition. The half maximum rate of CL increase was obtained with 1.5 ${\mu}m$ NADH or 55 ${\mu}m$ NADPH in hepatocytes and 6 ${\mu}m$ NADH or 30 ${\mu}m$ NADPH in plasma membranes. Measurement of these NADPH values required the presence of a NADPH-regenerating system. With NADPH the maximal rate obtained was 10 fold higher than with NADH. NADPH and NADH could produce CL when having access from either side of the membrane. They seemed to react with the identical acceptor because NADH-induced CL was also inhibited by $NADP^+$. The characteristics of ${\cdot}O_{2}^{-}-formation$ produced by pyridine nucleotide will be discussed.

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재생중인 흰쥐 간세포의 조직화학적 미세구조 관찰 (Observation of Histochemical Ultrastructure in Regenerating Rat Liver)

  • 최치용;손성향;유창규;최임순
    • Applied Microscopy
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    • 제18권2호
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    • pp.205-217
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    • 1988
  • An ultrastructural study of hepatocyte proliferation in the regenerating rat liver has been made by means of the partial hepatectomy. And electron microscopic histochemistry of hepatocyte in the regenerating rat liver is studied through alkaline phosphatase reaction. The results are as follows: 1. When the regeneration of rat liver is induced by the partial hepatectomy, the prominent ultrastructural characteristics of hepatocyte are changes of the distribution of chromatin in nucleus, increase of the number of mitochondria and decrease of the size of them, development of rough endoplasmic reticulum, and transient decrease of glycogen granules in cytoplasm. 2. Alkaline phosphatase reaction products are appeared in the nucleus or rough endoplasmic reticulum of hepatocyte during the initial regeneration of liver as 24, 48 and 72 hour groups after partial hepatectomy. And these positive reaction are mainly increased in cytoplasm and plasma membrane of hepatocytes during 1, 2 and 3 week groups after partial hepatectomy. As 4 weeks passed after partial hepatectomy, these positive reaction is located in the sinusoidal epithelial cells or erythrocytes. With above results, we concluded that alkaline phosphatase was synthesized in the rough endoplasmic reticulum bounded ribosomes of regenerating hepatocyte, was transported to the plasma membrane of them, and then was transported in blood by the way sinusoidel epithelial cells.

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하고초, 감국, 갈근 약침이 비만쥐의 지질강하, 항산화 효과, TNF-${\alpha}$, IL-6 및 Leptin의 농도와 각종 장기의 해부조직학적 소견에 미치는 영향 (Effects of Hagocho(prunella vulgaris L.), Gamgook(chrysanthemum indicum L.) and Galgeun(pueraria Radix) aqua-acupuncture at gokji(LI-11) and Joksamri(ST-36) on lowering lipid effect, oxidative capacity, concentration of TNF-Effects of Hagocho(prunella vulgaris L.), Gamgook(chrysanthemum indicum L.) and Galgeun(pueraria Radix) aqua-acupuncture at gokji(LI-11) and Joksamri(ST-36) on lowering lipid effect, oxidative capacity, concentration of TNF-${\alpha}$, IL-6, Leptin and histological consideration in hyperlipidemic rat, IL-6, Leptin and histological consideration in hyperlipidemic rat)

  • 장현준;이향숙;이준무
    • Korean Journal of Acupuncture
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    • 제24권4호
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    • pp.201-219
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    • 2007
  • Objectives : We studied Effects of Hagocho(prunella vulgaris L.), Gamgook(chrysanthemum indicum L.) and Galgeun(pueraria Radix) aqua-acupuncture on the hyperlipidemic rat. methods : We investigated lowering lipid effect, oxidative capacity, concentration of TNF-${\alpha}$, IL-6, Leptin and histological consideration in hyperlipidemic rat. Forty male Sprague-Dawley rats weighing about 400g were divided into 5 groups of control, Ⅰ: Hagocho (prunella vulgaris L.)+Gamgook (chrysanthemum indicum L.) and Gokji aqua-acupuncture, Ⅱ: Hagocho(prunella vulgaris L.)+Gamgook (chrysanthemum indicum L.) and Joksamri aqua-acupuncture, Ⅲ: Hagocho(prunella vulgaris L.)+Galgeun(pueraria Radix)and Gokji aqua-acupuncture and Ⅳ: Hagocho (prunella vulgaris L.)+Galgeun(pueraria Radix) and Joksamri aqua-acupuncture. Results : Contents of plasma ${\beta}$-lipoprotein, contents of IL-6, TNF-${\alpha}$ and leptin, Plasma triglyceride and glucose, plasma total cholesterol and LDL-cholesterol, liver total cholesterol, liver triglyceride, plasma and liver TBARS, free fatty acids showed a tendency to decrease in the aqua-acupuncture groups compared to those of control group. The activities of GOT and GPT showed no significantly different in all treatment groups. Values of super oxide dismutase, glutathione peroxidase and catalase activity showed a tendency to increase in the aqua-acupuncture groups. Histological consideration of heart, kidney and liver in the aqua-acupuncture groups showed slight vasodilation and fat accumulation compared to those of normal rat. Conclusions : These results indicated that prunella vulgaris L., chrysanthemum indicum L. and pueraria Radix aqua-acupuncture at gokji(LI11) and Joksamri(ST36) suppressed adipose tissue mass and lipid peroxidation and increased antioxidant system in hyperlipidemic rat.

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