• Title/Summary/Keyword: rat lens aldose reductase activity

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Inhibitory Effects of Ginseng Components on Rat Lens Aldose Reductase Activities (인삼성분의 Rat Lens Aldose Reductase 활성에 대한 억제효과)

  • Kim, Hack-Seang;Park, Ung-Yang;Seong, Yeon-Hee
    • Journal of Ginseng Research
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    • v.20 no.1
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    • pp.106-110
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    • 1996
  • The present study was undertaken to elucidate the effects of ginseng components on rat lens aldose reductase activity. Ginseng total saponin (GTS) exhibited inhibitory activities on rat lens aldose reductase in a dose-dependent manner. Among ginsenosides, Rf and Rgl showed potent inhibitory activities on rat lens aldose reductase. Lipid soluble fraction also inhibited rat lens aldose reductase activities. These data suggest that ginseng components inhibit rat lens aldose reductase activity in vitro.

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Coumarins from Angelica gigas Roots having Rat Lens Aldose Reductase Activity

  • Lee, Sang-Hyun;Jung, Sang-Hoon;Lee, Yeon-Sil;Shin, Kuk-Hyun
    • Biomolecules & Therapeutics
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    • v.10 no.2
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    • pp.85-88
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    • 2002
  • Systematic fractionation of Angelica gigas roots led to the isolation of linear coumarins such as decursinol angelate, decursin and nodakenin. These compounds were tested for their effects on rat lens aldose reductase. Nodakenin was demonstrated to exhibit significant inhibition of rat lens aldose reductase activity with $IC_{50}$ value of $7.33\Mu\textrm{M}$.

Hyperin, An Aldose Reductase Inhibitor from Acanthopanax senticosus Leaves

  • Lee, Sang-Hyun;Jung, Sang-Hoon;Lee, Yeon-Sil;Shin, Kuk-Hyun
    • Natural Product Sciences
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    • v.9 no.1
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    • pp.4-6
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    • 2003
  • Hyperin from the leaves of Acanthopanax senticosus was tested for its effect on rat lens aldose reductase and demonstrated to exhibit a significant inhibition of rat lens aldose reductase activity with $IC_{50}$ value of $2.63\;{\mu}M$.

Effects of Sparganii Rhizoma Processed on Rat Lens Aldose Reductase and Anti-oxidant Activities (흑삼릉의 수치에 따른 Aldose Reductase 및 항산화활성에 미치는 영향)

  • 정상훈;신국현;신현경;임순성
    • YAKHAK HOEJI
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    • v.48 no.5
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    • pp.291-296
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    • 2004
  • Sparganium stoloniferum Buch.-Ham. (Sparganiaceae) is a perennial herb which has been used as treatments for menstrual disorder, galactagogue and endometriosis in folk remedies. The Sparganii Rhizoma processed, heated with vinegar, used to different purpose compared with origin in medicine. In order to estimate the different effects between processed (PSR) and unprocessed (USR) Sparganii Rhizoma, extraction and systemic fractionation of the rhizomes were archived. Inhibitory effects of the extracts as well as the fractions of PSR and USR on rat lens aldose reductase and on rat platelet aggregation were investigated for the prevention or the treatment of chronic diabetic complications. Their antioxidant effects, measured using lipid peroxidation of liver tissue and radical scavenging activities on 1,1-diphenyl-picrylhydrazyl in vitro, were evaluated. The most of biological activities tested except rat platelet aggregation, fractions from PSR were shown to exhibit stronger activities than those from USR. It seems that caused by changing of the chemical components by heating process in conditioned with acetic acid. Compounds isolated were shown to significant inhibitory activity on rat lens aldose reductase. Inhibitory activity of compounds isolated on rat lens aldose reductase have been tested in vitro. $IC_{50}$/ (6.31 $\mu$M) of cerebroside I (V) was nearly equal to that (6.32 $\mu$M) of a reference compound, tetrametbylene glutaric acid (TMG).

Esculetin, a Coumarin Derivative, Inhibits Aldose Reductase Activity in vitro and Cataractogenesis in Galactose-Fed Rats

  • Kim, Chan-Sik;Kim, Junghyun;Lee, Yun Mi;Sohn, Eunjin;Kim, Jin Sook
    • Biomolecules & Therapeutics
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    • v.24 no.2
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    • pp.178-183
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    • 2016
  • Naturally occurring coumarin compounds have received substantial attention due to their pharmaceutical effects. Esculetin is a coumarin derivative and a polyphenol compound that is used in a variety of therapeutic and pharmacological strategies. However, its effect on aldose reductase activity remains poorly understood. In this study, the potential beneficial effects of esculetin on lenticular aldose reductase were investigated in galactose-fed (GAL) rats, an animal model of sugar cataracts. Cataracts were induced in Sprague-Dawley (SD) rats via a 50% galactose diet for 2 weeks, and groups of GAL rats were orally treated with esculetin (10 or 50 mg/kg body weight). In vehicle-treated GAL rats, lens opacification was observed, and swelling and membrane rupture of the lens fiber cells were increased. Additionally, aldose reductase was highly expressed in the lens epithelium and superficial cortical fibers during cataract development in the GAL rats. Esculetin reduced rat lens aldose reductase (RLAR) activity in vitro, and esculetin treatment significantly inhibited lens opacity, as well as morphological alterations, such as swelling, vacuolation and liquefaction of lens fibers, via the inhibition of aldose reductase in the GAL rats. These results indicate that esculetin is a useful treatment for galactose-induced cataracts.

Screening for Aldose Reductase Inhibitory Activity of Extracts of the Marine Plants from Korea (한국산 해산식물의 추출물로부터 Aldose Reductase 억제활성의 검색)

  • Lee, Yeon-Sil;Lee, Sang-Hyun;Kim, Bak-Kwang;Shin, Kuk-Hyun
    • ALGAE
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    • v.19 no.4
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    • pp.349-352
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    • 2004
  • We examined MeOH extracts of the marine plants from Korea for their inhibitory activities on rat lens aldose reductase in vitro. Among the MeOH extracts tested, the extracts of Enteromorpha prolifera, Ecklonia cava, Pelvetia siliquosa and Salicornia herbacea exhibited a rat lens aldose reductase inhibition (IC$_{50}$: 3.04, 8.84, 11.42 and 4.99 $\mu$g-ml$^{-1}$, respectively) in vitro.

Aldose Reductase Inhibitory Activity of Methanol Extracts from the Korean Plants

  • Jung, Sang-Hoon;Lim, Soon-Sung;Lee, Sang-Hyun;Lee, Yeon-Sil;Shin, Kuk-Hyun;Kim, Yeong-Shik
    • Natural Product Sciences
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    • v.9 no.1
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    • pp.34-37
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    • 2003
  • We examined methanol extracts prepared from the Korean plants for their inhibitory effects on rat lens aldose reductase (RLAR) activity in vitro. Among 41 plant extracts, the extracts of Lagerstroemia indica, Punica granatum, Eurya japonica, Liquidambar styraciflua, and Vitis coignetiae exhibited highest inhibitory potency, even more potent than tetramethylene glutaric acid (TMG), known as one of typical aldose reductase inhibitors $(IC_{50}\;value,\;0.51\;{\mu}g/ml)$. Especially, the extract of Lagerstroemia indica (Lythraceae), exhibited the most inhibitory potency $(IC_{50}\;value,\;0.069\;{\mu}/ml)$ on RLAR.

Isoflavonoids from the Rhizomes of Belamcanda chinensis and Their Effects on Aldose Reductase and Sorbitol Accumulation in Streptozotocin Induced Diabetic Rat Tissues

  • Jung, Sang-Hoon;Lee, Yeon-Sil;Lee, Sanghyun;Lim, Soon-Sung;Kim, Yeong-Shik;Shin, Kuk-Hyun
    • Archives of Pharmacal Research
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    • v.25 no.3
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    • pp.306-312
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    • 2002
  • Aldose reductase, the key enzyme of the polyol pathway, is known to play important roles in the diabetic complication. The inhibitors of aldose reductase, therefore, would be potential agents for the prevention of diabetic complications. To evaluate active principles for the inhibition of aldose reductase from the rhizomes of Belamcanda chinensis, twelve phenolic compounds were isolated and tested for their effects on rat lens aldose reductase. As a result, isoflavones such as tectorigenin, irigenin and their glucosides were found to show a strong aldose reductase inhibition. Tectoridin and tectorigenin, exhibited the highest aldose reductase inhibitory potency, their $IC_{50}$ values, being $1.08{\times}10^{-6}{\;}M{\;}and{\;}1.12{\times}10^{-6}{\;}M$, respectively, for DL-glyceraldehyde as a substrate. Both compounds, when administered orally at 100 mg/kg for 10 consecutive days to streptozotocin-induced diabetic rats, caused a significant inhibition of sorbitol accumulation in the tissues such as lens, sciatic nerves and red blood cells. Tectorigenin showed a stronger inhibitory activity than tectoridin. From these results, it is suggested that tectorigenin is attributed to be a promising compound for the prevention and/or treatment of diabetic complications.

Aldose Reductase Inhibition Effect of PhenolicvCompounds Isolated from Paulownia coreana Bark

  • Kim, Jin-Kyu;Lee, Yeon-Sil;Lim, Soon-Sung;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.38 no.2
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    • pp.159-164
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    • 2010
  • Nine compounds, caffeic acid, naringenin, apigenin, luteolin, kaempferol, verbascoside, isoverbascoside, isocampneoside II, and cistanoside F, were isolated from the EtOAc and n-BuOH fractions of P. coreana bark. The structures of these compounds (1-9) were elucidated by spectroscopic methods and literature data. All the isolates were subjected to in vitro bioassay to evaluate their inhibitory activity against rat lens aldose reductase. Among these, compounds 6 and 8 indicated the significant inhibitory activity on rat lens aldose reductase with $IC_{50}$ values of 2.67 and 5.59 ${\mu}M$, respectively. Especially, The inhibition activity of acteoside was 3.9 times better than that of quercetin as a positive control (10.6 ${\mu}M$). These results suggested that phenylethanoid glycosides are likely to be the potential compounds for the prevention and/or treatment of diabetic complications.

Inhibitory Effects of Naturally Occurring Flavonoids on Rat Lens Aldose Reductase

  • Jung, Sang-Hoon;Kang, Sam-Sik;Shin, Kuk-Hyun;Kim, Yeong-Shik
    • Natural Product Sciences
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    • v.10 no.1
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    • pp.35-39
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    • 2004
  • Aldose reductase (AR), the key enzyme of the polyol pathway, is known to play important roles in the diabetic complications. The inhibitors of AR, therefore, would be potential agents for the prevention of diabetic complications. In order to clarify the structural requirements of flavonoids for aldose reductase inhibitory activity, thirty flavonoids were examined. Among the thirty flavonoids, flavonols such as quercetin (5), reyneutrin (7), quercitrin (9), isoquercitrin (11), and avicularin (14) were found to exhibit much stronger AR inhibition. Lonicerin (10), amentoflavone (27) and sophoraflavanone B (30) were also showed strong inhibitory activity. Especially, quercitrin and reyneutrin exhibited the most inhibitory potency on rat lens (RL) AR. The results suggested that flavonol having the 7-hydroxyl and/or catechol moiety at the B ring exhibit strong activity. In addition, flavonols having 3-O-monosaccharide also showed stronger inhibition than free flavonols at the 3-position. These results suggested that quercitrin and reyneutrin are attributed to be the promising compounds for the prevention and/or treatment of diabetic complications.