• 대한치과의사협회지
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• 제15권12호
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• pp.957-962
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• 1977

The author has observed the tissue reaction of the absolute alcohol infection of rat oral mucosa. 0.5ml absolute alcohol was injected subcutaneously on the mucobuccal fold of rat. And the rats were sacrifieced at intervals of one day, 3rd, 1 week, 2 week and 4 week after alcohol injection. The microscopic tissue sections were made and stained with hematoxylin and eosin. The results were are as follows; 1. Degeneration and shrinkage of fibroblasts and coagulative necrosis were observed one day to and three day after alcohol injection. 2. Although coagulative necrosis and tissue degeneration occurred, the inflammatory infiltration was not prominent especially there were scarcely any polymorphonuclear leukocytes in that field. 3. Granulation tissue with moderate small round cell infiltration were replaced the necrotic area at one week after injection and the fibroblast proliferate into the granulation tissue at two week group. 4. At four week after injection, the damaged area recovered by fibroblastic proliferation and collage formation, but there were

• 대한소아치과학회지
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• 제24권1호
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• pp.41-57
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• 1997

The present study investigated the effects of hyperbaric oxygen therapy on periodontal wound healing of replanted rat tooth. 80 rats (Sprague-Dawley strain) weighting $130{\pm}5gm$ were selected and divided into experimental and control group, each group consisting of 40 rats. Rats were administered 0.4% ${\beta}$-aminoproprionitrile for 5 days to achieve gentle tooth extraction. The maxillary first molars were extracted under anesthesia with pentobarbital, washed in sterile distilled water, treated with bacterial collagenase to remove collagen fibers on the root surfaces. After washing in water overnight, the mesial root surface were demineralized by application of citric acid, washed, dried and stored at $4^{\circ}C$. Immediately after tooth extraction and bleeding control, the treated molars extracted previously from other rats were replanted. The experimental group was exposed to hyperbaric oxygen at 2.5 atm. for 2 hrs. a day during experimental period. Eight animals of each group were sacrificed 1, 3, 6, 8, 10 days after reimplantation of teeth by intracardiac perfusion with 4% paraformaldehyde. The replanted molars and surrounding tissues were cut, demineralized, dehydrated and embedded in paraffin. Sections were stained with azan, toluidine blue and hematoxylin. Some other sections were stained by means of immunostaining achieved by the avidinbiotin complex method. The results as follows; 1. Experimental group showed fast healing of gingival epithelium. 2. Macrophage and newly formed blood vessels appeared early in the gingival connective tissue of experimental group. 3. Experimental group showed fast, abundant fibroblast proliferation and regularity of collagen fiber. 4. In both group, collagen was distributed along the collagen fiber. The distribution was strong and regular in the experimental group. 5. In the regenerated periodontal ligament of experimental group, fibers showed regular arrangement and invaded root surface fast.

• The Journal of Korean Physical Therapy
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• 제26권6호
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• pp.418-424
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• 2014

Purpose: This study was performed to investigate the effect of joint mobilization on pain relief and cartilage repair in an induced osteoarthritis rat model by analyzing the expression of heat shock protein 70 in articular cartilage. Methods: MIA was injected into SD rats to induce osteoarthritis. These rats were divided into 4 groups: control group (n=30), no further treatment after the MIA injection ; experimental group I(n=30), performed swimming exercise after the MIA injection experimental group II (n=30), underwent joint mobilization after the MIA injection and experimental group III (n=30), performed swimming exercise and underwent joint mobilization after the MIA injection. For the histologic and pathophysiologic evaluation, safranin-O staining and for the immunohistochemical evaluation, the expression of HSP 70 in articular cartilage was analyzed 1, 7, 14, and 21 days after the MIA injection. Results: The inflammatory response and loss of tissue declined in experimental groups I and II over time, whereas the greatest decreases were noted in experimental group III. In the articular cartilage, low expression of HSP 70 was observed in every group on day 1, whereas HSP 70 expression was elevated on days 7 and 14 in experimental groups II and III. After 21 days, experimental group II displayed the strongest positive reaction, whereas HSP 70 was higher in experimental group III at this time point compared to that after 14 days. Conclusion: Our results showed that swimming exercise and joint mobilization had positive effects on pain relief and histologic and functional recovery in an induced osteoarthritis rat model.

• 한국응용과학기술학회지
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• 제4권1호
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• pp.1-8
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• 1987

This experiment is carried out to study influence of vitamin $B_6$ on serum lipids of rat fed with oxidized oil by heat. The experimental animals use 35 male Sprague-Dawley rats weighing 100 ${\pm}$ 2g. They are divided into 5 groups and fed to experimental diets which are added to 10%, 15%, 20% oxidized oil by heat in addition to 0.01%, 0.04%, 0.06% vitamin $B_6$. Oxidized oil by heat is prepared from the soybean oil by heating at $180^{\circ}C$ for 50 hours. After feeding for 4 weeks, lipid concentrations of sera are as follows; 1. Body weights are higher in experimental diet groups than control diet group. 2. Liver weights are higher in experimental diet groups than control diet group, but spleen weights are lower in experimental diet groups than control diet group. 3. The contents of serum total cholesterol, free cholesterol, HDL-cholesterol are lower in experimental diet groups than control diet group. 4. The contents of serum triglyceride are significantly higher in experimental diet groups than control diet group. 5. The contents of serum phospholipid are lower in experimental diet groups than control diet group.

• 한국식품영양과학회지
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• 제13권4호
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• pp.451-458
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• 1984

18% casein식(食)을 대조식이(對照食餌)로 하여 분지쇄(分枝?) 아미노산(Leu. lie. Val.)을 첨가(添加)한 실험식이(實驗食餌)의 간부분절제후(肝部分切除後)의 Rat에 대한 영양학적(營養學約) 효과(效果)를 검토(檢討)하였다. 자유채취(自由攝取)시키면, 실험식이군(寶驗食餌群)은 대조식이군(對照食餌群)에 비하여 6일(日)째까지 식이채취량(食餌攝取量)이 적지만, 8 일(日)째부터는 증가하고 체중증가(體重增加)도 그와 평행하였다. 14 일(日)째에 간(肝)의 부분절제(部分切除)를 행하면, 7 일후의 체중(體重)은 식이채취량(食餌攝取量)은 동일(同一)하면서도 실험식이군(實驗食餌群)에서 유의(有意)하게 증가(增加)하였다. 또한 간부분절제후(肝部分切除後) 5 일(日)째의 간재생중량(肝再生重量) 및 간재생지수(肝再生指數)도 유의(有意)하게 증가(增加)를 나타내었다. 이상으로 보아, 분지쇄(分枝?)아미노산 첨가식이(添加食餌)는 재생간(再生肝) Rat에 대하여 체중증가(體重增加) 및 간재생(肝再生) 보호작용(促進作用)에 있어서 영양학적(營養學論) 효과(效果)가 있다는 것이 인정(認定)된다.

• 대한방사선기술학회지:방사선기술과학
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• 제39권3호
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• pp.451-459
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• 2016

방사선 사고 및 방사선 치료 등 방사선에 의한 과피폭의 피해를 줄이기 위해 방사선방호제 개발연구는 활발히 진행되고 있다. 이에 화학적 합성물이 아닌 항산화, 항암, 면역 증강에 효과적인 것으로 알려진 백하수오를 이용하여 방사선방호효과를 확인하였다. 백하수오 에탄올추출물을 Sprague Dawley Rat (SD Rat)에 14일간 1일 1회 경구 투여하고, 7 Gy X-ray를 조사한 후 1일, 4일, 7일, 21일의 시간 변화에 따른 혈구성분, 비장 지수의 변화 및 간과 자궁의 조직변화를 관찰하였다. 실험결과 백하수오 에탄올추출물을 섭취한 실험군의 백혈구 수치(p < 0.05)와 비장 지수(p < 0.05)가 대조군보다 회복이 빠른 것을 확인하였다. 간 조직에서는 핵의 응축, 세포질의 팽창, 염증세포의 침윤이 감소하였으며, 자궁샘 조직은 세포고사가 감소한 것을 확인하였다. 위의 결과를 토대로 백하수오 에탄올추출물은 방사선 조사에 따른 혈구 및 장기의 피해를 줄일 수 있는 새로운 방사선방호제로써 유용할 것으로 기대되며, 방사선 사고와 같은 비상관리 분야에 적절한 시사점을 제공할 수 있다.

• 한방재활의학과학회지
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• 제26권3호
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• pp.31-49
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• 2016

Objectives The study was designed to evaluate the healing effects of Joaguihwan (JGH) extract on Anti-oxidation, Anti-inflammatory in RAW 264.7 Cells and factors related with bone metabolism in skull fractured Rat. Methods The fracture healing effect of JGH was measured by scavenging activities of1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and nitric oxide (NO) in RAW 264.7 cells. The inhibitory effect against the production of inflammatory mediators including interleukin-$1{\beta}$ (IL-$1{\beta}$), interleukin-6 (IL-6), tumor necosis factors-${\alpha}$ (TNF-${\alpha}$) expression was inhibited in RAW 264.7 cells was experimented using JGH. The effects of JGH on healing fractured rats was measured by osteocalcin, calcitonin, CTXII, TGF-${\beta}$, BMP-2, Insulin, ALP in the serum. and was checked every 3 weeks from 0 week to 6week using x-ray. Results 1. DPPH free radica and ABTS scavenging activity of JGH were increased according to concentration of JGH in RAW 264.7 Cells. 2. In the experiment, NO, IL-$1{\beta}$, IL-6, TNF-${\alpha}$ all showed decrease, in general. Especially NO and IL-$1{\beta}$ showed significantly decrease at a concentration of 10, 100 (${\mu}g/ml$). 3. In the production of osteocalcin in the serum, JGH 200, 400 mg/kg experimental group showed significant increased effect at 2 weeks. 4. In the production of calcitonin in the serum. JGH 200 mg/kg experimental group showed significant increased effect at 4, 6 weeks. JGH 400 mg/kg experimental group showed significant increased effect at 2, 4, 6 weeks. 5. In the production of CTX, TGF-${\beta}$, BMP-2 in the serum, experimental group showed increased effect. but no significant effect. 6. In the production of insulin in the serum. JGH 200, 400 mg/kg experimental group showed significant decrease effect at 2, 4, 6 weeks. 7. In the production of ALP in the serum. JGH 200 mg/kg experimental group showed significant increased effect at 2, 4, 6 weeks. JGH 400 mg/kg experimental group showed significant increased effect at 4, 6 weeks. 8. In the change of X-ray, the experimental group showed better healing effects on skull fractured rats than control group. Conclusions From above results, JGH showed healing effect on Anti-oxidation, Anti-inflammatory in RAW 264.7 Cells, factors related with bone metabolism in the serum of skull fractured rat and x-ray, which is expected to be applied in clinics.

• 대한한방내과학회지
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• 제31권3호
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• pp.457-466
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• 2010

Objective : In benign prostatic hyperplasia, dihydrotestosterone acts as a potent cellular androgen and promotes prostate growth. Inhibiting enzyme $5{\alpha}$-reductase that is involved in the conversion of testosterone to the active form dihydrotestosterone reduces this excessive prostate growth. The mechanism on benign prostatic hyperplasia is substantiating evidence to support the clinical value in the evaluation of therapeutic efficacy. In this study, we investigated the effects of Lygodium japonicum on cyto-pathological alterations and expression of $5{\alpha}$-reductase in the rat model of benign prostatic hyperplasia induced by castration and testosterone treatment. Methods : Sprague-Dawley rats were treated with testosterone after castration for induction of experimental benign prostatic hyperplasia, which is similar to human benign prostatic hyperplasia in histopathological profiles. Lygodium japonicum as an experimental specimen, and finasteride as a positive control, were administered orally. The prostates were evaluated by histopathological changes and testosterone levels. Also, the prostates were observed by hematological alterations of AST, ALT, ${\gamma}$-GTP, BUN and creatinine. Results : The rats treated with Lygodium japonicum showed a diminished range of luminal cell and duct epithelial cell damage. The stromal elements and connective tissue proliferation of Lygodium japonicum treated group as compared to the control group decreased. Conclusions : These findings suggest that Lygodium japonicum may protect the glandular epithelial cells. We concluded that Lygodium japonicum could be a useful remedy agent for treating the benign prostatic hyperplasia.

• 대한한방내과학회지
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• 제28권4호
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• pp.779-790
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• 2007

Objective : Although chronic non-bacterial prostatitis is a common disease, it is very difficult to treat effectively. GLS (Gleditsiae Spina) has traditionally been used in treatment of urinary tract inflammation and voiding disturbance. In this study, we investigated the therapeutic effects and action mechanism of GLS in the rat model of non-bacterial prostatitis induced by castration and testosterone treatment. Method : We observed four experimental objects of normal group, control group, testosterone group, and GLS group. Rats were treated with 17$\beta$-estradiol after castration for induction of experimental non-bacteral prostatitis, which is similar to human chronic prostatitis in histophatological profiles. GLS and testosterone were administered as an experimental specimen and a positive control, respectively. The prostates were evaluated by histopahological parameters including the epithelial score and epithelio-stromal ratio for glandular damage. Also, the prostates were observed by hematological alterations of WBC, RBC, hemoglobin, hematocrit and platelet. Results : While prostates of control rats revealed severe acinar gland atrophy and stromal proliferation, the rats treated with GLS showed a diminished range of tissue damage. Epithelial score was improved in GLS over that of the control. The epithelial-stromal ratio was lower in GLS when compared to that of the control. Conclusion : These findings suggest that GLS may protect the glandular epithelial cells. We concluded that GLS could be a useful remedy agent for treating chronic non-bacterial prostatitis.

• Clinical and Experimental Reproductive Medicine
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• 제25권3호
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• pp.305-313
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• 1998

Steroid hormone is known to cause the dynamic changes of mammalian uterus during reproductive cycle. However there is little information about the effect of estrogen (E) and progesterone (P) on the expression of various transcription factors involved in gene expression. Thus the present study was designed to demonstrate E and/or P-induced expression of c-Fos, CREB, ATF and HSP70 in rat uterus. Rats, ovariectomized (OVX) for two weeks, were divided into 6 experimental groups, 1) OVX, 2) OVX+V, 3) OVX+E, 4) OVX+P, 5) OVX+E+V, 6) OVX+E+P, and western blotting assay for nuclear extract and immunohistochemical staining were carried out for each experimental group. Treatment of E $(10{\mu}g)$ showed to increase the expression of c-Fos, CREB, ATF, and HSP70, and maximal expression was occured at $3\sim6$ hr after E administration. P (1mg) also increased, but much less than E, the expression of c-Fos, ATF, and HSP70. However, P did not reveal any effect on the expression CREE. P treatment 4 hr after E injection decreased c-Fos, CREB, and ATF expression, but did not show any change in the E-induced HSP70 expression. In immunohistochemical study c-Fos-, CREB-, and ATF-immunoreactivities were confined to the cells of luminal epithelium of uterine endometrium. These results suggest that proliferation and differentiation of rat uterus during reproductive cycle may mediated via expression of transcription factors, such as c-Fos, CREB, ATF, and HSP70.